固始鸡孵化期间蛋黄胆固醇、粗脂肪和锌含量分析   总被引：2，自引：0，他引：2  

李国喜  康相涛  韩瑞丽  孙桂荣  李朝云  梅承君 《湖北农业科学》2006,45(4):497-499

对固始鸡种蛋孵化期间蛋黄中胆固醇、粗脂肪和锌含量的测定表明,胆固醇含量0～6胚龄明显升高,以后出现阶段性降低;粗脂肪含量0～6胚龄无明显变化,6胚龄以后逐渐降低;锌含量0～3胚龄和6～12胚龄明显升高,3～6胚龄和12～21胚龄降低.  相似文献   

Toward the Identification of Novel Antimicrobial Agents: One-Pot Synthesis of Lipophilic Conjugates of N-Alkyl d- and l-Iminosugars     

Anna Esposito  Daniele D&#x;Alonzo  Stefano D&#x;Errico  Eliana De Gregorio  Annalisa Guaragna 《Marine drugs》2020,18(11)

In the effort to improve the antimicrobial activity of iminosugars, we report the synthesis of lipophilic iminosugars 10a–b and 11a–b based on the one-pot conjugation of both enantiomeric forms of N-butyldeoxynojirimycin (NBDNJ) and N-nonyloxypentyldeoxynojirimycin (NPDNJ) with cholesterol and a succinic acid model linker. The conjugation reaction was tuned using the established PS-TPP/I₂/ImH activating system, which provided the desired compounds in high yields (94–96%) by a one-pot procedure. The substantial increase in the lipophilicity of 10a–b and 11a–b is supposed to improve internalization within the bacterial cell, thereby potentially leading to enhanced antimicrobial properties. However, assays are currently hampered by solubility problems; therefore, alternative administration strategies will need to be devised.  相似文献   

Soluble Klotho protein suppresses THP-1-derived foam cell formation     

LIU Wei  LI Lin  LIU Jia-ni  SHI Jing  HONG Tian  CHEN Xiu-juan 《园艺学报》2020,36(5):816-821

AIMTo investigate the role of soluble Klotho protein in THP-1-derived foam cell formation. METHODSTHP-1 monocytes were induced into macrophages by treatment with 160 nmol/L phorbol myristate acetate for 48 h, and then were divided into 6 groups: negative control group (THP-1-derived macrophages), positive control group [THP-1-derived foam cells induced by oxidized low-density lipoprotein (ox-LDL) for 48 h], and 25, 50, 100 and 200 μg/L soluble Klotho protein groups (THP-1-derived macrophages pretreated with soluble Klotho protein at the indicat?ed concentraions for 2 h and then induced by ox-LDL for 48 h). Lipid droplets in cytoplasm were observed by oil red O staining. The cholesterol outflow rate was detected by scintillation counting technique. The content of intracellular total cholesterol, free cholesterol and cholesterol ester was detected by enzyme fluorescence analysis. The expression of acyl-coenzyme A:cholesterol acyltransferase 1 （ACAT1） and ATP-binding cassette transport?er A1 (ABCA1) at mRNA and protein levels was determined by RT-qPCR and Western blot, respectively. RESULTSOil red O staining and lipid mass quantification showed that THP-1-derived foam cell formation was dose-dependently suppressed by soluble Klotho protein. The cholesterol efflux rate of THP-1-derived foam cells was increased by soluble Klotho protein in a dose-dependent manner (P<0.05). In addition, soluble Klotho protein decreased the expression of ACAT1 and increased the expression of ABCA1 in a dose-dependent manner (P<0.05). CONCLUSION The soluble Klotho protein inhibits THP-1-derived foam cell formation in a dose-dependent manner by down-regulating the expression of ACAT1 and up-regulating the expression of ABCA1.  相似文献   

黄鳝体长与血清胆固醇含量关系的分析     

张廷华  曾凡坤  李念  陈亮  田晓花 《湖北农业科学》2007,46(1):144-146

测定了不同体长黄鳝血清的胆固醇含量,发现黄鳝体长(x)与血清胆固醇含量(y)之间存在极显著的直线相关,回归方程y=6.059x 2.164,相关系数r=0.628 6.  相似文献   

中药“蛋必增”对产蛋鸡血清及蛋黄胆固醇的影响   总被引：2，自引：1，他引：2  

付明哲  刘璐  李岩  武和平  赵发苗  陈晓强  袁福汉 《甘肃农业大学学报》2003,38(2):224-226,230

应用中草药添加剂“蛋必增”分别按1 %、2 % 和0 % 的添加量饲喂产蛋鸡,表明“蛋必增”能激活肝脏7ɑ-羟化酶活力,降低产蛋鸡血清中胆固醇和鸡蛋胆固醇含量。“蛋必增”对产蛋鸡胆固醇代谢有一定影响作用。  相似文献   

植物甾醇对团头鲂生长、血脂和肝胰脏抗氧化功能的影响     

张严伟  阮剑均  温超  刘文斌  周岩民 《水产科学》2012,31(4):192-196

选取体质量(74.52±1.03)g的团头鲂480尾,随机分成4组,每组4个重复,每个重复30尾,分别投喂添加0(对照组)、20mg/kg(试验Ⅰ组)、40mg/kg(试验Ⅱ组)和80mg/kg(试验Ⅲ组)植物甾醇的配合饲料,进行为期81d的饲养试验。试验结果表明,40、80mg/kg植物甾醇可提高团头鲂质量增加率及特定生长率(P0.05),其中40mg/kg组效果更佳,对肌肉粗蛋白含量影响不显著,对肌肉粗脂肪含量有一定提高(P0.05);添加40mg/kg植物甾醇显著降低了81d血浆低密度脂蛋白胆固醇水平(P0.05)。在本试验条件下,添加40mg/kg植物甾醇效果最佳。  相似文献   

Assessment of dietary lecithin and cholesterol requirements of mud crab, Scylla serrata, megalopa using semi-purified microbound diets   总被引：1，自引：0，他引：1  

M.-H. HOLME  P.C. SOUTHGATE  & C. ZENG 《Aquaculture Nutrition》2007,13(6):413-423

The effects of varying dietary lecithin and cholesterol levels on growth, development and survival of mud crab, Scylla serrata, megalopa were evaluated using six semi‐purified, microbound diets formulated to be iso‐energetic and containing three levels of supplemental lecithin (0, 20 and 40 g kg⁻¹ diet dry weight) and two levels of supplemental cholesterol (0 and 7 g kg⁻¹ diet dry weight). Fifteen megalopa were reared individually in each treatment and the nutritional value of diets was assessed on basis of mean dry weight and mean carapace width of newly settled first crab stage, as well as development time to the first crab stage and overall survival. A significant interaction between supplemental dietary lecithin and supplemental dietary cholesterol was found for final mean dry weight of newly settled crabs, and highest survival (60%) was recorded for megalopa fed diets containing the highest levels of dietary lecithin (39.7–44.1 g kg⁻¹) (diet 5 and 6) regardless of whether diets were supplemented with cholesterol; this rate of survival was identical to that of megalopa fed live Artemia nauplii. The results indicate that supplemental dietary cholesterol may not be essential for mud crab megalopa when fed diets containing sufficient levels of supplemental dietary phospholipids.  相似文献   

The Effect of Lecithin Supplementation on the Biochemical Profile and Morphological Changes in the Liver of Rats Fed Different Animal Fats     

Lechowski R  Bielecki W  Sawosz E  Krawiec M  Kluciński W 《Veterinary research communications》1999,23(1):1-14

Sixty-four Wistar rats were divided into 8 equal groups and kept for 36 days in individual boxes. Three of the groups were given full synthetic diets containing various animal fats: beef tallow, pork fat or fish oil. A control group was fed the diets without the fat. The other four groups were fed the same diets with lecithin supplementation. At the end of experiment, sections taken from the liver were stained with haematoxylin–eosin and Sudan III to indicate fat infiltration. Liver enzyme levels, total bilirubin, albumin and two products of lipd metabolism were measured in serum from all the animals. The addition of lecithin to the diets did not influence the level of enzymes in the serum except in rats fed the diet containing beef tallow. A relationship between the type of diet, lecithin supplementation and the total cholesterol concentration in serum was observed. The concentration of HDL-cholesterol was only influenced by lecithin supplementation and that of triglycerides by the type of fat in the diet. The addition of lecithin to the diet was associated with proliferation of Kupffer cells, and an increased number of binuclear cells. Fatty degeneration of hepatocytes was less pronounced in all groups following lecithin supplementation. Lecithin supplementation of the diet did not elicit any pathological lesions and may be considered as a hepatocyte protector. This favourable effect of lecithin was most marked in the group of rats fed the fish oil.  相似文献   

洛伐他汀(Lovastatin)对鸡蛋和鸡血浆中胆固醇含量的影响     

刘金存  汪劲能  刘燕杰 《中国家禽》2007,29(22):24-26

将44只新扬州鸡随机平均分成4组.对照组用基础日粮,试验组在饲喂基础日粮的同时分别添加0.015%、0.03%和0.06%的洛伐他汀(Lovastatin),研究洛伐他汀对鸡蛋和血浆中胆固醇含量的影响,试验期为5周.试验结果表明:洛伐他汀能显著降低产蛋鸡血浆和鸡蛋中胆固醇水平(P＜0.05),但对产蛋率的影响不显著(P＞0.05).  相似文献   

Effect of dietary Schizochytrium microalga oil and fish oil on plasma cholesterol level in rats          下载免费PDF全文

T. Komprda  O. Škultéty  S. Křížková  G. Zorníková  V. Rozíková  R. Krobot 《Journal of animal physiology and animal nutrition》2015,99(2):308-316

The purpose of the study was to test the hypothesis that the dietary oils with different content of n‐3 polyunsaturated fatty acids (PUFA) eicosapentaenoic acid (EPA) + docosahexaenoic acid (DHA) affect plasma lipid level in rats in a different degree. The diets with 6% of fish oil (FO) and Schizochytrium microalga oil (SchO; EPA+DHA content in the diets 9.5 + 12.3 and 2.6 + 29.5% of the sum of total fatty acids, respectively) were used; the diet with 6% of safflower oil (high content of n‐6 PUFA linoleic acid, 65.5%; EPA+DHA content 0.7 + 0.9%) was used as a control. The difference between FO and SchO was established only in the case of plasma triacylglycerol (TAG) level: plasma TAG of the FO‐fed rats did not differ from the control rats (p > 0.05), while SchO decreased (p < 0.05) plasma TAG to 46% of the control. On the other hand, FO and SchO decreased (p < 0.05) total plasma cholesterol (TC) in rats in the same extent, to 73% of the control. Regarding the underlying mechanisms for the TC decrease, both SchO and FO up‐regulated hepatic Insig‐1 gene (181 and 133% of the control; p < 0.05), which tended (p = 0.15 and p = 0.19 respectively) to decrease the amount of hepatic nSREBP‐2 protein (61 and 66% of the control). However, neither SchO nor FO influenced hepatic 3‐hydroxy‐3‐methyl‐glutaryl‐CoA reductase gene expression (p > 0.05); SchO (but not FO) increased (p < 0.05) low‐density lipoprotein receptor mRNA in the liver. It was concluded that the decrease of total plasma cholesterol might be caused by an increased cholesterol uptake from plasma into the cells (in the case of SchO), but also by other (in the present study not tested) mechanisms.  相似文献