Comparison on growth performance,body coloration changes and stress response of juvenile river catfish,Pangasius hypophthalmus reared in different tank background colour     

Siti Umey Syahirah Mat Nawang  Fui Fui Ching  Shigeharu Senoo 《Aquaculture Research》2019,50(9):2591-2599

The effects of different tank background colours (green, black and white) on the growth performance and body coloration of juvenile river cafish, Pangasius hypophthalmus, were examined in this study. The experimental fish with an initial body weight (BW) 0.13 ± 0.05 g and total length (TL) 2.54 ± 0.37 cm were used, and groups of 15 fish per tank were randomly distributed. The experiment was conducted for 20 days as a triplicate for each treatment. Survival, growth in BW, body weight gain (BWG), total length (TL), total length gain (TLG), specific growth rate (SGR), condition factor, feed conversion ratio (FCR), swimming speed, body coloration and stress response were evaluated. River catfish reared in white and green tanks attained significantly higher BW, BWG, TL, TLG and SGR compared to the black tank. The white tank attained significantly better FCR compared to the black tank. River catfish showed the most active swimming behaviour in the green tank (5.49 ± 1.32 cm/s) while the least in the white tank (3.37 ± 0.93 cm/s). Body coloration of river catfish in the black tank showed almost pure black body coloration (75.59 ± 9.08), followed by the green tank (126.23 ± 17.46) and the white tank (148.19 ± 10.51). However, there was no significant difference in the stress level of river catfish reared in white and black tanks and insignificant to those reared in the green tank. The findings in this study showed that the white and green background colour tanks can be used for promoting growth of river catfish culture while the black background colour tank can enhance body coloration that is suitable as ornamental fish.  相似文献   

饲料中添加不同水草粉对克氏原螯虾生长、消化酶、抗氧化酶活性及色泽的影响     

张亚文  成永旭  李晨露  王源源  侯受权  李京昊  奚业文  朱传忠  李嘉尧 《水产学报》2022,46(8):1414-1426

【目的】为研究饲料中添加不同水草粉对克氏原螯虾生长、消化酶、抗氧化酶活性及色泽的影响,【方法】本研究 通过在饲料中分别添加15%的伊乐藻、轮叶黑藻、苦草、金鱼藻和喜旱莲子草5种水草粉(分别标记为Diet 2~6组),对照组饲料无水草粉添加（标记为Diet 1组）,对克氏原螯虾幼虾进行为期60 d的养殖实验。【结果】结果显示：水草组(Diet 2~6组)成活率(SR)与Diet 1组无显著差异,Diet 3~5组增重率(WGR)及特定生长率(SGR)均与Diet 1组无显著差异,而Diet 3组肝胰腺指数(HSI)显著高于Diet 1组。在肝胰腺中,Diet 2~6组脂肪酶(LPS)活性显著高于Diet 1组,且Diet 5组LPS和α-淀粉酶(α-AL)活性最高,而胃蛋白酶活性为Diet 2组最高,Diet 2~3及5组纤维素酶(CL)活性显著高于Diet 1组;在肠道中,Diet 2~6组4种消化酶活性均低于Diet 1组。Diet 3~6组头胸甲和肌肉总类胡萝卜素、头胸甲虾青素含量及卵巢中总类胡萝卜素、虾青素、叶黄素和β-胡萝卜素含量均显著高于Diet 1组。Diet 2~6组头胸甲及卵巢的红度(a*值)和黄度(b*值)均显著高于Diet 1组,而亮度(L*值)却低于Diet 1组。在肝胰腺中,Diet 2~6组总超氧化物歧化酶(T-SOD)活性均显著高于Diet 1组且在Diet 5组最高,Diet 4组总抗氧化能力(T-AOC)活性最高,Diet 2组丙二醛(MDA)含量最高;血清中,Diet 6组T-AOC活性最高,但各组间T-SOD活性和MDA含量无显著差异。【结论】综上,添加15%轮叶黑藻、苦草和金鱼藻水草粉到饲料中,对克氏原螯虾生长无负面影响,有利于体内类胡萝卜素、虾青素的积累,改善克氏原螯虾头胸甲和卵巢色泽的同时提升抗氧化能力。  相似文献   

桑突变体Cty-Ym叶片蛋白质的双向电泳及质谱分析   总被引：2，自引：0，他引：2  

谈建中  刘美娟  张国英  李新梅  吴志平 《蚕业科学》2005,31(1):8-13

为探讨桑树叶色突变体CtyYm变异的分子机理,采用蛋白质组学分析技术,从突变体和对照桑中提取并分离叶片蛋白质,获得了IEF/SDSPAGE双向电泳图谱,在CBB染色条件下,检测到比较清晰的蛋白质点184个;经PDQuest软件分析,发现两者蛋白质点的相对含量有36处存在显著差异(±2倍),尤其是突变体中蛋白质点SSP2801的相对含量仅为对照桑的258%,经胶内酶切、LCESIQTOFMS分析、肽质量和碎片离子检索分析,鉴定该蛋白组分为RuBisCO大亚基,推测突变体CtyYm叶色黄化可能与RuBisCO大亚基含量减少有关。  相似文献   

染色法测定、计算机解译农田土壤中大孔隙数量的研究   总被引：5，自引：0，他引：5  

吴华山  陈效民  邱琳  沃飞 《水土保持学报》2006,20(3):145-149

利用染色和计算机解译的方法来确定太湖地区3种主要水稻土（白土、黄泥土和乌栅土）的优势流路径和大孔隙的分布状况。将土壤染色后，所拍摄的剖面染色图片经过Photoshop 8．0和ArcGIS8．0处理，将所染的蓝色由深到浅平均分成10个等级，以此分析土壤剖面中大、小孔隙的比例。分析结果表明：（1）3种土壤剖面的优势流深度相差不大，均在80cm左右。但因为乌栅土的地下水位较高（最高可达70cm）。所以优势流对鸟栅土的地下水影响最大。（2）土壤剖面中不同深处的染色百分比与该处土壤容重有显著的相关性。土壤容重越大，染色百分比就越小。（3）不同土壤剖面大孔隙的数量和孔隙体积相差很大。在白土和黄泥土的所有土壤层次上以及乌栅土的剖面上第三至第四层，蓝色最深的两个等级之和的百分比即是剖面上该层〉1mm的大孔隙含量。另外在乌栅土剖面上的第一至第二层〉1mm的大孔隙含量数值与经染色后产生蓝色最深的3个等级百分数之和相当。  相似文献   

新嘎拉苹果果实着色试验     

于立杰  梁春莉  王庆玲 《北方园艺》2010,(18):41-43

通过套袋、应用反光膜、喷施增红剂、摘叶等措施,研究其对新嘎拉苹果含糖量、花青苷、着色果率的影响。结果表明:4种措施都明显提高了果实色泽,且増红剂可显著提高果实红色,反光膜可增大树冠内照幅度,摘叶可减少叶片遮荫造成的果面绿斑。  相似文献   

Interactive effects of dietary astaxanthin and cholesterol on the growth,pigmentation, fatty acid analysis,immune response and stress resistance of kuruma shrimp (Marsupenaeus japonicus)     

Weilong Wang  Manabu Ishikawa  Shunsuke Koshio  Saichiro Yokoyama  Mahmoud A. O. Dawood  Md. Sakhawat Hossain  Amr I. Zaineldin 《Aquaculture Nutrition》2019,25(4):946-958

A 56‐day feeding trial was carried out to evaluate the interactive effects of chemically synthesized astaxanthin (Ax) and cholesterol (CHO) on the performances of kuruma shrimp juveniles. Six experimental diets containing 2 levels of Ax (0 and 0.6 g/kg diet) and 3 levels of CHO (0, 6 and 20 g/kg diet) were formulated in 2 × 3 factorial design. The results indicated that the interactions between dietary CHO and Ax were not significant on survival, growth performances, body proximate analysis, immune response and freshwater stress test. Significantly, better pigmentation performances and Ax contents of different parts of juveniles (whole body, muscle, carapace and head) were observed in higher Ax (Ax_0.6)‐supplemented groups. Meanwhile, interactions of dietary Ax and CHO were also found on colour reading parameters and whole‐body Ax contents. In addition, juveniles fed with the diet containing Ax at 0.6 g/kg diet in addition to CHO at 0, 6 and 20 g/kg diet showed better performances on digestive enzyme activities (protease, lipase and amylase) of hepatopancreas than 0 g Ax/kg diet supplemented with CHO at 0, 6 and 20 g/kg diet. It was concluded that dietary Ax and CHO functioned interactively.  相似文献   

Effect of diets on carotenoid content,body coloration,biochemical composition and spermatophore quality in the “red cherry” shrimp Neocaridina davidi (Caridea,Atyidae)     

Ana Laura Tomas  Daniela Eliana Sganga  Agustina Marciano  Laura Susana Lpez Greco 《Aquaculture Nutrition》2020,26(4):1198-1210

In aquarium industry trade markets, Neocaridina davidi, the “red cherry” shrimp, is one of the most attractive ornamental shrimps due to the striking reddish coloration of females. Five commercial diets formulated for ornamental species were evaluated over a 90‐day period in N. davidi males and females, based on survival, biochemical composition, body weight, carotenoid content and body coloration. Furthermore, histological and histochemical analyses of the male reproductive system were performed to evaluate the spermatophore quality. In all treatments, survival was >80%, and protein content was similar in both female and male shrimps. Lipid content was higher in females and males fed on a diet having the highest lipid level. Carotenoid content was higher and body coloration was brighter in females and males fed on a diet containing the highest carotenoid level. Histological and histochemical analyses of the spermatophore structure and composition showed no differences among diets, suggesting that the five commercial diets are adequate for satisfactory growth and spermatophore quality of N. davidi. The diverse sources and quantities of carotenoids in the highest carotenoid level diet render it more suitable for colour enhancement of this species.  相似文献   

基于花青素苷合成和呈色机理的观赏植物花色改良分子育种   总被引：7，自引：0，他引：7  

戴思兰  洪艳 《中国农业科学》2016,49(3):529-542

花色是观赏植物最重要的品质性状之一,是植物自然进化过程中最具适应意义的表型性状,也是表观遗传学研究的重要内容。花青素苷是使花朵呈色的重要色素之一,被子植物中约有80%的科的花朵颜色由花青素苷决定;迄今从自然界分离和鉴定出的花青素苷多达600种,主要由6种花青素苷元衍生而来。花青素苷合成途径是迄今为止研究得最为清楚的植物次生代谢途径之一,它的合成首先取决于类黄酮代谢途径的生成,花青素苷种类的多样性则源于其不同分支途径的形成,在花青素苷元基本骨架上不同位置取代基的差异形成了多种多样的花青素苷。在花青素苷生物合成过程中,分支点酶的竞争机制和关键酶的底物特异性使花青素苷的种类及相应的花色表型具有种属特异性。花青素苷合成后需要转运到液泡中被包裹成色素体,植物细胞中的液泡积累和贮存色素体的能力是影响花青素苷呈色的重要因素,因此,花青素苷在花瓣中的最终呈色还受液泡pH、助色素含量以及金属离子的络合作用等多种细胞内因素的影响。目前,已经在多种植物中获得了与花青素苷合成及呈色相关的结构基因和调节基因,并解析了其功能,成功获得了一些转基因花卉,但是这些基因调控表达的机制,包括转录水平和转录后水平的调控、DNA序列本身的差异和DNA甲基化修饰的调控机制等仍不清楚,转基因植株花色改良的程度也很有限,对于如何将这些机制应用于花色改良的转基因育种也是一个前沿的课题。花青素苷对园艺作物器官呈色机制的解析有助于对花朵呈色机制的理解,观赏植物中花色形成机理的研究对于园艺作物器官呈色机制的解析同样具有重要的参考价值。因此,本文以观赏植物为例,从花青素苷合成分支途径形成的机理、花青素苷生物合成途径的遗传调控机理以及影响花青素苷呈色的主要因素及其遗传调控机理3个方面,对影响植物花朵呈色的机制进行了综述,并对近年来基于花青素苷代谢和呈色机理的花色改良分子设计育种,尤其是国际上广泛关注的蓝色花育种进行了梳理和总结,以期为定向培育具有新奇花色的观赏植物新品种提供参考。  相似文献   

套袋对妃子笑荔枝果实着色的影响   总被引：18，自引：3，他引：18  

陈大成  王卫华 《华南农业大学学报》1999,20(4):65-69

１９９７,１９９８年在广东省荔枝科普基地矮化栽培荔枝园采用硫酸纸袋、无纺布袋、纱网袋、报纸袋、牛皮纸袋对妃子笑荔枝进行套袋处理,结果表明：硫酸纸袋、无纺布袋可以使果皮提前１０ｄ着色,通过影响花青苷、类黄酮、可溶性酚等酚类物质和叶绿素含量,不同程度地改善果皮色泽品质,促进了苯丙氨酸解氨酶（ＰＡＬ）活性;报纸袋、牛皮纸袋显著抑制果皮花青苷合成,抑制了苯丙氨酸解氨酶（ＰＡＬ）活性;纱网袋促 色效果不明显  相似文献   

分光光度法测定茶叶中总黄酮含量     

王丽丽  林清霞  宋振硕  陈林 《茶叶学报》2021,62(1):1-6

黄酮类与儿茶素类物质对茶叶感官品质和生物活性起重要作用,因此建立一种茶叶中总黄酮(包括黄酮类和儿茶素类)含量的测定方法很有必要.采用70％甲醇提取茶样,以芦丁为对照品,利用亚硝酸钠-硝酸铝-氢氧化钠溶液显色,在510 nm下测定吸光度,建立测定茶叶中总黄酮含量的分光光度法,并对其进行方法学考察.应用该法测定了绿茶、白茶、乌龙茶和红茶4个茶类15个茶样中总黄酮含量,采用福林酚法测定其茶多酚含量,并比较二者相关性.结果表明,芦丁质量浓度在0.2～1.0 mg·mL-1范围内与吸光度线性关系良好,回归方程为Y=1.2465X+0.0074(R2=0.9997).方法学考察结果表明,该方法精密度、重复性和稳定性的吸光度RSD为1.17％、2.92％和6.01％,平均加标回收率为84.35％(RSD=4.15％).15个茶样中总黄酮含量范围为5％～10％,总黄酮含量规律为:绿茶、白茶>乌龙茶>红茶.福林酚比色法测得茶多酚含量范围为7％～15％.双变量相关性分析显示,总黄酮含量与茶多酚含量呈极显著正相关,Pearson相关系数达0.874.由此表明,该方法操作简便,精密度、重复性与稳定性好,可用于茶叶总黄酮含量测定.  相似文献