桑突变体Cty-Ym叶片蛋白质的双向电泳及质谱分析   总被引：2，自引：0，他引：2  

谈建中  刘美娟  张国英  李新梅  吴志平 《蚕业科学》2005,31(1):8-13

为探讨桑树叶色突变体CtyYm变异的分子机理,采用蛋白质组学分析技术,从突变体和对照桑中提取并分离叶片蛋白质,获得了IEF/SDSPAGE双向电泳图谱,在CBB染色条件下,检测到比较清晰的蛋白质点184个;经PDQuest软件分析,发现两者蛋白质点的相对含量有36处存在显著差异(±2倍),尤其是突变体中蛋白质点SSP2801的相对含量仅为对照桑的258%,经胶内酶切、LCESIQTOFMS分析、肽质量和碎片离子检索分析,鉴定该蛋白组分为RuBisCO大亚基,推测突变体CtyYm叶色黄化可能与RuBisCO大亚基含量减少有关。  相似文献   

Genomic regions associated with the degree of red coloration in pericarp of rice (Oryza sativa L.)     

Yanjun Dong  Jianlong Xu  Ke Xiao  Yongjuan Zhang  Junzhi Zhang  Lijun Luo  M. Matsuo 《Journal of Cereal Science》2008

The degree of red coloration (DRC) in pericarp of rice depends on the content of flavonoid compounds which have beneficial health effects for humans. In this study, 182 backcross-recombinant inbred lines (BILs) derived from Koshihikari (white pericarp)/Kasalath (red pericarp)//Koshihikari were used to detect the genomic regions associated with DRC through the QTL mapping approach. As a result, a total of four genomic regions were found to associate with DRC on chromosomes 1, 7, 9 and 11, respectively. Interestingly, the two genomic regions having the largest effects corresponded to previously characterized Rc and Rd genes on chromosome 7 and 1, respectively. In addition, two novel genomic regions having minor effects on DRC and located on chromosomes 9 and 11, respectively, are reported here for the first time. These results and the identification of tightly linked molecular markers that flank the genomic regions provide an opportunity for marker-aided improvement of red coloration in pericarp of rice.  相似文献   

pH对三七块根花色苷颜色呈现和降解的效应     

赵昶灵  崔秀明  卢其能 《云南农业大学学报(自然科学版)》2008,23(6)

用分光光度法在体外研究了pH对三七块根花色苷呈色和降解的效应,结果表明:该花色苷在可见光区的吸收光谱和降解速率均具独特的pH依赖性。在pH2.0,该花色苷呈现最强烈的红色。随着pH从0增加到13.0,该花色苷在可见光区的最大吸收波长(λvis max)依次出现红移、蓝移,然后消失,在可见光区最大吸收波长处的吸光值(Aλvis max)呈现为一条单峰曲线,唯一的峰在pH2.0处。当原始pH值被恢复到2.0后,如果原始pH值≤6.0,花色苷的红色均被恢复得更浓烈,λvis max不同程度地趋向532 nm,Aλvis max增加;如果原始pH值≥7.0,花色苷的红色根本不能被恢复,λvis max几乎不变,Aλvis max仍然维持低水平。在15℃,黑暗中,该花色苷在pH0~6.0条件下均随时间而降解,在pH2.0时的降解速度最慢,当pH≤3.0时,该花色苷在总体上降解缓慢;此外,该花色苷的降解过程几乎符合一级反应动力学。本文可为三七块根颜色呈现的机理探索及其色素的开发、利用提供参考。  相似文献   

木、竹、藤材的变色防治   总被引：5，自引：0，他引：5  

吕文华  江泽慧  吴玉章  任海青  费本华 《世界林业研究》2006,19(4):38-44

对木材变色的机理、类型、成分和影响因素, 木材变色的预防和消除以及生物防治等研究的最新成果进行了概括总结, 综合论述了木材、竹材和藤材的变色防治研究现状, 得出以下几点结论:(1)低毒或无毒、高效、多功能的防变色剂的研制开发仍是木材工业变色防治研究的重要研究方向。(2)开展色变脱除, 恢复已变色材原有颜色和价值方面的研究很有必要。(3)以菌治菌的生物防治, 污染小, 成本低, 应加强其理论研究和实践应用。(4)通过热处理或光辐射等手段, 诱导木、竹、藤材变色, 进行调色处理, 将成为新的研究方向。(5)与木材相比, 竹材、藤材变色的研究落后, 尤其是藤材变色, 应得到进一步加强。  相似文献   

Genetic variation within coat color genes of MC1R and ASIP in Chinese brownish red Tibetan pigs     

Huirong MAO  Jun REN  Nengshui DING  Shijun XIAO  Lusheng HUANG 《Animal Science Journal》2010,81(6):630-634

Melanocortin receptor 1 (MC1R) and agouti signaling protein (ASIP) are two major genes affecting coat color phenotypes in mammals, and inactivation mutations in the MC1R gene are responsible for red coat color in European pig breeds. Conversely, the gain‐of‐function ASIP mutations block MC1R signaling and lead to the production of red pheomelanin. Chinese Tibetan pigs have three types of coat color phenotypes, including brownish red, solid black and black with patches of brownish red and white. Herein, we investigated variations of the MC1R and ASIP genes in Tibetan pigs. The results showed that the brownish red Tibet pig had the dominant black MC1R allele (E^D1). No loss‐of‐function mutation in MC1R responsible for red coat color in European breeds was observed in this breed. No causal mutation for the red coat color phenotype was found in the coding sequence of the ASIP gene. A novel missense mutation c.157G > A was firstly identified in exon 2 of ASIP, which was further genotyped in 285 pigs from five Chinese breeds and three Western breeds having different coat color phenotypes. Nearly all pigs were GG homozygotes. In conclusion, no functional variant responsible for brownish red coloration was found in the coding region of MC1R and ASIP in Tibetan pigs.  相似文献   

早熟灵对温州蜜柑提早着色及增进品质的效应   总被引：2，自引：0，他引：2  

邓伯勋  章文才 《果树学报》1994,(3)

8月中旬对温州蜜柑成年树喷施一次200—300mg·1~(-1) 的早熟灵,能提早果实着色13—16d,可溶性固形物提高11%左右,柠檬酸含量降低23%—36%,可溶性固形物与酸比明显增大35%—50%,维生素C也显著增加10%—12%.6月中旬喷施一次100—150mg·l~(-1)的早熟灵能促进果实明显膨大,产量有所增加,并能增加 30%—50%的新梢生长量,从而可以缓和早熟温州蜜柑结果与抽梢的矛盾.研究结果表明,早熟灵对温州蜜柑提早着色、增进品质、提早上市、增加收入、开拓国内外市场具有显著效应.  相似文献   

分光光度法测定茶叶中总黄酮含量     

王丽丽  林清霞  宋振硕  陈林 《茶叶学报》2021,62(1):1-6

黄酮类与儿茶素类物质对茶叶感官品质和生物活性起重要作用,因此建立一种茶叶中总黄酮(包括黄酮类和儿茶素类)含量的测定方法很有必要.采用70％甲醇提取茶样,以芦丁为对照品,利用亚硝酸钠-硝酸铝-氢氧化钠溶液显色,在510 nm下测定吸光度,建立测定茶叶中总黄酮含量的分光光度法,并对其进行方法学考察.应用该法测定了绿茶、白茶、乌龙茶和红茶4个茶类15个茶样中总黄酮含量,采用福林酚法测定其茶多酚含量,并比较二者相关性.结果表明,芦丁质量浓度在0.2～1.0 mg·mL-1范围内与吸光度线性关系良好,回归方程为Y=1.2465X+0.0074(R2=0.9997).方法学考察结果表明,该方法精密度、重复性和稳定性的吸光度RSD为1.17％、2.92％和6.01％,平均加标回收率为84.35％(RSD=4.15％).15个茶样中总黄酮含量范围为5％～10％,总黄酮含量规律为:绿茶、白茶>乌龙茶>红茶.福林酚比色法测得茶多酚含量范围为7％～15％.双变量相关性分析显示,总黄酮含量与茶多酚含量呈极显著正相关,Pearson相关系数达0.874.由此表明,该方法操作简便,精密度、重复性与稳定性好,可用于茶叶总黄酮含量测定.  相似文献   

欧李果实着色期糖酸含量的变化规律   总被引：1，自引：0，他引：1  

王鹏飞  张建成  曹琴  纪薇  穆霄鹏  杜俊杰  李志芳  韩向峰 《山西农业科学》2014,(1):25-28

欧李果实着色期是碳水化合物合成和转化的关键时期,而糖酸比是决定果品风味和加工性能的重要指标之一。以3个欧李新品种为材料,分析了果实上色过程可溶性糖、淀粉、酸含量的动态变化。结果表明,随着果实着色程度增加,3个品种可溶性糖含量均呈持续增加趋势,淀粉含量呈持续下降趋势。在转色过程中,农大6号和农大7号果实可滴定酸含量呈持续上升趋势,农大6号增幅较快,而农大7号增速缓慢,农大9号酸含量在着色过程中呈下降趋势。3个品种成熟时糖酸比为农大7号最高、农大6号次之、农大9号最低。生产中应根据鲜食或加工的需求,把握最佳采收时期。  相似文献   

Endocrine correlates of gender and throat coloration in the southern ground-hornbill (Bucorvus leadbeateri)     

Juan SCHEUN  Sophie NELLER  Nigel C. BENNETT  Lucy V. KEMP  Andre GANSWINDT 《Integrative zoology》2021,16(2):189-201

The southern ground-hornbill (SGH) is a cooperatively breeding bird endemic to eastern and southern Africa, but is endangered in its southern distributional range. The national conservation restoration program harvests redundant chicks for captive breeding and reintroduction; with sexing and social grouping of the species evaluated by throat-skin coloration, with adult males displaying a completely red color compared to dark blue within the red observed in adult females. However, recent findings indicate that dominant and subordinate adult males exhibit patches of blue throat-skin. To optimize SGH management practices, it is vital to determine the role of red and blue coloration, as well as the possible drivers thereof. As a prerequisite, an enzyme immunoassay for monitoring fecal androgen metabolite (fAM) concentrations in SGH was established. Following this, fresh fecal samples were collected from 78 SGH, of various demographics and origin, across 12 captive institutions, to determine whether fAM concentrations differ between blue (B), partially blue (sB), and fully red (R) throat-skin colored SGH. Furthermore, fAM concentrations were compared between males housed in different social groups of different age and sex classes. Individual median fAM concentrations of B, sB, and R adult males did not differ significantly but were considerably higher in B and sB males compared to R males. Social dynamics within captivity, for example, dominance, played no role as a driver of male gonadal activity or throat skin coloration. The results of the study indicate that androgens and apparent social dynamics are not primary determinants of throat coloration in male SGH.  相似文献   

染色法测定、计算机解译农田土壤中大孔隙数量的研究   总被引：5，自引：0，他引：5  

吴华山  陈效民  邱琳  沃飞 《水土保持学报》2006,20(3):145-149

利用染色和计算机解译的方法来确定太湖地区3种主要水稻土（白土、黄泥土和乌栅土）的优势流路径和大孔隙的分布状况。将土壤染色后，所拍摄的剖面染色图片经过Photoshop 8．0和ArcGIS8．0处理，将所染的蓝色由深到浅平均分成10个等级，以此分析土壤剖面中大、小孔隙的比例。分析结果表明：（1）3种土壤剖面的优势流深度相差不大，均在80cm左右。但因为乌栅土的地下水位较高（最高可达70cm）。所以优势流对鸟栅土的地下水影响最大。（2）土壤剖面中不同深处的染色百分比与该处土壤容重有显著的相关性。土壤容重越大，染色百分比就越小。（3）不同土壤剖面大孔隙的数量和孔隙体积相差很大。在白土和黄泥土的所有土壤层次上以及乌栅土的剖面上第三至第四层，蓝色最深的两个等级之和的百分比即是剖面上该层〉1mm的大孔隙含量。另外在乌栅土剖面上的第一至第二层〉1mm的大孔隙含量数值与经染色后产生蓝色最深的3个等级百分数之和相当。  相似文献