印度南瓜与中国南瓜种间杂交试验     

叶飞华  张明方  金永祥 《蔬菜》2018,(5):13-17

为了获取印度南瓜与中国南瓜的"种间自交系",为印度南瓜与中国南瓜的种间杂交提供桥梁亲本,在获得印度南瓜与中国南瓜种间F1的基础上,以印度南瓜作为回交亲本,通过2次回交后再连续5次自交,获得了可育的BC_2F_6种子。种间自交系BC_2F_5可与印度南瓜正反交,也可用中国南瓜花粉对其授粉得到正常数量F1种子,但用其花粉对中国南瓜授粉仍难获得杂交种子。种间自交系与印度南瓜杂交获得的F1具有实际应用价值。  相似文献   

山葡萄种间杂交F_1～F_4代对霜霉病抗性的遗传分析   总被引：2，自引：1，他引：1  

宋润刚  郑永春  路文鹏  沈育杰  范书田  杨义明  李晓红  林兴桂 《果树学报》2008,25(1):33-39

1973—2006年对我国东北山葡萄抗寒的种质资源(品种、品系)与不抗寒的欧亚种酿造葡萄品种进行种间杂交、回交和重复杂交。共定植42个组合杂种苗,定植成活7680株。观察42个组合7680株杂种苗F1￣F4代对霜霉病抗性的的抗性分离,共分离出抗病(2￣3级)3747株,占杂种苗总株数48.8%。其中抗病×抗病的10个组合共分离出抗病2303株、占69.3%,分别高于抗病×不抗病、中等感病×中等感病、中等感病×高感病、高感病×高感病的组合的23.2、29.2、45.3和54.8个百分点。遗传规律是:山葡萄种间杂交后代F1￣F4对霜霉病抗性分离,表现为连续分布,倾向于抗病亲本,后代群体抗病性主要由亲本抗病性决定,杂交组合中抗病亲本越多,分离出的抗病单株越多,为多基因控制的数量性状遗传、"抗病基因"有累加效应、呈显性遗传给后代。已选育出抗霜霉病、抗寒、可酿造干红山葡萄酒的新品种左优红、品系94-7-75、94-8-168、95-2-482和2002-1-135。  相似文献   

松材线虫和拟松材线虫的杂交遗传差异研究^*   总被引：2，自引：0，他引：2       下载免费PDF全文

刘伟  杨宝君 《林业科学研究》1994,7(5):469-473

运用杂交方法对来自南京、四川、日本、法国和加拿大等地的松材线虫和拟松材线虫共１０个株系作遗传分析，结果表明中国的松材线虫和拟松材线虫能杂交成功，但后代数量少且大多不孕，两者间遗传差异较大，这两种线虫各株系间无明显差异。当中国线虫分别与日本、法国、加拿大株系杂交时，无论种间或种内均有差异，前者差异更显著。种间杂交中多数后代雌虫较难与父本加交产生子代幼虫。种间杂交在产生正常后代的同时也产生１０％～６０  相似文献   

四种紫胶虫雄性外生殖器观察及初步杂交试验   总被引：1，自引：4，他引：1       下载免费PDF全文

陈晓鸣  王绍云  毛玉芬  冯颖 《林业科学研究》1992,5(2):236-238

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Study on ecology ofFraxinus mandshurica     

Wang Yihong 《林业研究》1995,6(3):61-64

The reference and recent working results about ecology of ash (Fraxinus mandshurica) were reviewed, which including: distribution range, suitable site, phonology, growth, photosynthesis, productivity, natural regeneration effect and mixture effect. Synthesizing above, it was suggest that the ecological characters of ash such as distribution range, limited factors, growth site etc., as well as its biology characters, features in forest, should be considered as an important basis to develop this species by different regeneration ways such as natural regeneration, artificial regeneration and natural regeneration by man-help. And experiment in artificial regeneration showed that the mixture of ash with larch has benefit to being free from or alleviating frost injury and enhancing growth  相似文献   

Testing the Efficiency of Pollination Bag Materials     

N. J. McAdam  J. Senior  M. D. Hayward 《Plant Breeding》1987,98(2):178-180

Crossing bags manufactured from three grades of polyester and three types of paper bags were tested for their permeability to grass pollen. Bags were placed on L. perenne plants of known PGI isozyme genotype. Any pollen passing through the crossing bag and pollinating the plant was detected by assaying its progeny for PGI isozymes. The results obtained allowed differentiation of the various materials for their suitability for pollen proofing.  相似文献   

Agronomic traits and gene containment capability of cleistogamous rice lines with the superwoman1-cleistogamy mutation     

Shinnosuke Ohmori  Hiroaki Tabuchi  Osamu Yatou  Hitoshi Yoshida 《Breeding Science》2012,62(2):124-132

Pollen-mediated transgene flow is a major concern for the production of genetically modified (GM) rice. Cleistogamy is a useful tool for preventing this form of gene flow. We previously identified the cleistogamous rice mutant superwoman1-cleistogamy (spw1-cls) and determined its molecular genetic mechanism. In the present study, we cultivated spw1-cls over five years to examine effects of cleistogamy on agronomic traits. Simultaneously, we cultivated cleistogamous backcross lines created by continuous backcrossing with “Yumeaoba” (a japonica cultivar) as the recurrent parent and by application of a DNA marker. In these experimental cultivations, spw1-cls and its backcross lines showed almost equal or slightly lower, but acceptable, agronomic traits compared with each control line. We also conducted natural crossing tests in paddy fields to assess the gene containment capability of spw1-cls. In a series of field experiments, there was no natural crossing between spw1-cls (pollen donor) and pollen recipient lines, but the wild-type donor and recipient lines were crossed. Thus, the cleistogamy of the spw1-cls mutation is able to inhibit natural crossing effectively, without significant loss of commercial benefits, such as yield. We conclude that spw1-cls cleistogamy is a practical tool for gene containment in GM rice cultivation.  相似文献   

22年生柳杉双列杂交子代生长量遗传分析及选择     

黄信金 《福建林业科技》2012,(1):5-8,16

对22年生柳杉双列杂交子代生长性状的遗传变异规律和相关性进行研究,结果表明:树高、胸径和材积等主要生长性状在杂交组合间的差异随林龄的增大而增大,22年生时达1%极显著水平;生长性状年度间相关性均达5%显著水平或1%极显著水平,相关系数值逐渐增大,林龄大于7年生时,相关系数值大多超过0.50;树高、胸径和材积的一般配合力方差和特殊配合力方差均达5%显著水平或1%极显著水平;加性方差在总方差分量所占比率较大,生长性状主要受加性遗传控制,同时,生长性状受立地条件影响较大,优良杂交组合在比较适合的立地条件更能发挥其速生潜能;选出优良杂交组合9个,22年生树高、胸径和材积的平均值分别为9.54 m、14.62 cm和0.08297 m3,树高、胸径和材积的平均遗传增益分别为3.07%、3.77%和9.22%。所选优良材料可用于柳杉第2代种子园建设。  相似文献   

大刍草(Zea mexicana schard)×自交系K169选系的核型和SSR分析     

石海春  乔晓  柯永培  傅体华  余学杰  秦家友 《核农学报》2010,24(4):698-703

本研究以玉米自交系K169及其与大刍草(Zea mexicana schard)远缘杂交而成的5个选系为研究材料,对它们进行核型和SSR分析,以期从细胞学和分子水平的角度为玉米远缘杂交育种提供理论依据。结果表明,5个远缘选系和轮回亲本K169体细胞染色体数目均为2n=20,但在核型结构上却存在较大差异,其中自交系K169和远缘选系1191在第6染色体的短臂上有随体,1183和1189在第8染色体的短臂上有随体,1193和1195未发现带随体的染色体;选系1183、1193和1195的染色体长度比(最长/最短)与K169之间存在较大差异;选系1183和1195的核型类型与K169不一致。96对SSR引物在5个选系和轮回亲本K169中共检测出27个多态性位点,位点多态信息量(PIC)的平均值为0.724;基因型多样性(H′)的平均值为1.400,它们之间的遗传相似系数平均值为0.5791。综合分析表明,这些选系与其轮回亲本K169之间存在真实的遗传差异,是一批新的玉米种质资源。  相似文献   

地质条件对定向钻穿越的影响与应对措施   总被引：3，自引：0，他引：3  

李德选  王雪强  王军卫  王华良  任巍彪  王宁 《油气储运》2012,31(3):175-177,248

在长输油气管道工程设计中经常遇到黄土塬、游荡性大型河流、山体等不同地形地貌,如砾砂、卵石层、溶蚀性地层等多种地层条件及其组合。针对不同地形、地貌条件,对穿越曲线设计和泥浆工艺设计进行分析,结果表明:穿越曲线设计是定向钻穿越成功的基础;结合地形、地貌,设计具有针对性的穿越曲线是实现穿越工艺方案的关键;根据定向钻穿越地层的不同地层结构岩性,设计具有针对性的泥浆配比工艺参数,可以有效降低卡钻、抱钻及回拖阻力过大等风险;定向钻穿越时,灵活应用定向钻的单边钻、对向钻、水平钻穿越组合技术手段可解决传统技术手段难以解决的问题。  相似文献