An enzyme immunoassay to measure canine circulating fibronectin     

O'Neill S  Boothby JT  Feldman BF 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1986,15(1):22-26

A competitive enzyme immunoassay has been used to detect and quantitate fibronectin in canine plasma. In this test, purified fibronectin, bound to microtiter plates, competes with plasma fibronectin for the conjugated antibody, rabbit-anticanine, fibronectin-horseradish peroxidase. The assay could detect fibronectin in purified standards from 58 ng/ml to 580 microgram/ml. The range of 1-100 microgram/ml was linear for plasma samples diluted 1:10, allowing samples with fibronectin concentrations from 10-1000 microgram/ml to be easily measured by this method. The mean normal fibronectin concentration of 132 dogs, by this method, was determined to be 320 +/- 74 microgram/ml.  相似文献   

黑米天然黑(紫)色素的研究     

王景晨  王学增  王亚莉 《信阳农业高等专科学校学报》1992,(1)

特种稻黑米品种的糙米果皮中,含有大量的稀有天然黑(紫)色素。本文研究了这种色素的提取与分离方法、化学结构及其稳定性,并对其药理作用和应用前景作了简要分析。  相似文献   

Serum lipase determination in the dog: a comparison of a titrimetric method with an automated kinetic method     

Walter GL  McGraw P  Tvedten HW 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1992,21(1):23-27

An enzymatic, kinetic method for determining serum lipase activity was evaluated and compared to a standard manual method for use in dogs. The kinetic method was a commercial kit adapted for use on a tandem access clinical chemistry analyzer and utilized a series of coupled enzymatic reactions based on the hydrolysis of 1,2-diglyceride by lipase. The manual method was the Cherry-Crandall technique based on the titration of base against the acid formed by hydrolysis of an olive oil substrate by lipase. The correlation between the two methods was very good (r = 0.94). The reference range for 56 clinically healthy dogs assayed by the kinetic method was 90 to 527 U/L. Diseases associated with a greater than twofold elevation in serum lipase activity as determined by the kinetic method included pancreatitis, gastritis with liver disease, and oliguric renal failure with metabolic acidosis. In some cases, pancreatitis was seen with other clinical problems, such as gastroenteritis, diabetic ketoacidosis, duodenal mass, disseminated intravascular coagulation, and septic peritonitis. Diseases associated with serum lipase activity within the reference range or elevated less than twofold included gastritis, gastric ulcer, cholestasis, phenobarbital-induced hepatopathy, colitis, copper hepatopathy, abdominal hematoma, apocrine gland adenocarcinoma, and thrombocytopenia with pneumonia.  相似文献   

葡萄子油及原花青素研究与开发利用   总被引：14，自引：0，他引：14  

王辉宪  姜晖霞  王仁才  欧阳建文  熊兴耀 《果树学报》2005,22(5):542-547

综述了葡萄子油、葡萄子原花青素等天然产物的提取与保健作用的研究进展,原花青素的生物活性及药理研究的现状,葡萄子油成分分析方法和葡萄子,提取物中原花青素含量测定的方法;介绍了用超临界CO2萃取法制取高质量的葡萄子油和应用夹带剂提取葡萄子中的原花青素的方法。分析了葡萄子的综合开发的应用前景。  相似文献   

复合酶制剂对产蛋鸡生产性能的影响     

宋连喜  周丽荣 《家畜生态》2005,(3)

选用41周龄的海兰褐商品蛋鸡1200只,随机分成两组,每组600只。组内设3个重复,每个重复200只。对照组喂基础日粮,试验组喂基础日粮+0.1%蛋鸡用复合酶制剂,试验期28d。结果表明在产蛋鸡的基础日粮中添加0.1%的复合酶制剂可以使产蛋率平均提高6.12%(P<0.05),料蛋比降低11.69%(P<0.05)。说明在蛋鸡的基础日粮中添加适当的复合酶制剂能促进蛋鸡消化吸收,降低饲养成本,提高饲料转化率和经济效益。  相似文献   

农田沙漠化演变中土壤质量的生物学特性变化   总被引：9，自引：5，他引：9  

苏永中  赵哈林  张铜会  文海燕 《干旱区研究》2002,19(4):64-68

通过研究处于沙漠化过程中的科尔沁沙地旱作农田土壤酶活性与基础土壤呼吸活性，分析了土壤质量生物学特性的沙漠化演变。结果表明，土壤酶活性与基础土壤呼吸对沙漠过程中环境的变化反应敏感，随着沙漠化程度增加，与土壤物理化学肥力因素有关的过氧化氢酶、脲酶和碱性磷酸酶的活性和土壤呼吸活性随之下降。土壤酶活性和呼吸活性与土壤有机质和养分有极显著的相关，在一定程度上可以用酶活性和呼吸强度表征有机质和养分的状况及其数量的变异，因此，可以作为农田沙漠化演变中土壤质量特征变化的重要辅助指标。  相似文献   

镧素对镰刀菌Fusarium solani及其致病酶的影响   总被引：2，自引：0，他引：2  

慕康国  张文吉  胡林  张福锁 《农药学学报》2002,4(4):56-60

采用琼脂平板生长速率法及液体培养基培养测定了La对镰刀菌Fusarium solani的抑制作用和毒力,并测定了其对病菌胞外的果胶酶、蛋白酶和纤维素酶等几种致病酶活性的影响。结果表明,随着La2O3浓度升高,对病菌菌丝生长的抑制作用增强,对病菌的EC50和EC95分别为278.2和552.0 mg/L;在一定浓度范围内,La提高了单位量菌丝所产生3种致病酶的活性,但由于菌丝生长受到抑制,除蛋白酶外,病菌胞外致病酶果胶酶和纤维素酶的总量或总活性受到了抑制,降低了病菌的致病力。  相似文献   

亚精胺对猕猴桃采后抗氧化酶活性的影响   总被引：9，自引：0，他引：9  

闫瑞香  王仁才  关文强 《园艺学报》2002,29(2):173-175

 研究了不同亚精胺处理对猕猴桃采后抗氧化酶活性及保鲜效果的影响, 结果表明: 猕猴桃果实采后(8 ±2) ℃条件下贮藏12 d , SOD 活性达到高峰; POD、CAT、ASP 活性高峰相对SOD 推迟约13 d。不同亚精胺处理均不同程度地延缓了SOD 活性高峰的出现, 提高了SOD、CAT 的活性, 减少了MDA 的积累,降低了POD、ASP 的活性峰值, 延长了猕猴桃的贮藏保鲜期, 其中以Spd 与NAA 结合处理的效果最为显著。  相似文献   

Effects of angiotensin II-induced hypertension chemotherapy on the activity of interleukin-1β converting enzyme in transplanted intracerebral rat gliomas     

SHANG Zhan-ping  HOU Zhong-chi  ZHU Shi-gong  JI Guo-yi  WANG Jian-chun  ZHAO Wan 《园艺学报》2002,18(12):1521-1523

AIM: To investigate the activity of interleukin-1β converting enzyme in transplanted intracerebral rat gliomas under angiotensin II-induced hypertension chemotherapy. METHODS: The brain tumor model was produced in Wistar rats by stereotaxic inoculation of C6 glioma cells (1×10¹² /L). Tumor-bearing rats were treated with carmustine, teniposide and lisplatin (chemotherapy) during angiotensin II-induced hypertension. Then, the survival time of tumor-bearing rats, tumor blood flow, the concentration of drug, volume of gliomas and the activity of interleukin-1β converting enzyme in glioma were examined.RESULTS: The survival time of tumor-bearing rats was significantly longer in chemotherapy with angiotensin II-induced hypertension group than that of chemotherapy alone. In addition, regional tumor blood flow, the concentration of chemotherapeutic drug and the activity of interleukin -1β converting enzyme in transplanted rat gliomas were increased, while the volume of gliomas was decreased in hypertention chemotherapy group compared with chemotherapy alone. CONCLUSION: Chemotherapy with angiotensin II-induced hypertension has a enhancing effect on chemotherapy for improving the drug delivery to tumor tissue by a increased tumor blood flow and enhancing activity of interleukin -1β converting enzyme.  相似文献   

我国农药残留快速检测技术的研究与应用现状   总被引：21，自引：1，他引：21  

涂忆江 《农药科学与管理》2003,24(4):14-16

本文简述了目前农药残留快速检测技术的研究概况，分析了农药残留快速检测技术在我国的实际应用情况。  相似文献