增智金针菇液体发酵保健饮料的研究     

唐玉琴  徐济责 《吉林农业科技学院学报》2008,17(3):6-8

以金针菇菌丝体及其发酵液为主要原料,研制出金针菇液体发酵饮料。试验采用液体深层发酵技术,对金针菇菌丝体进行发酵培养,选出最佳发酵时间,以获得发酵液及菌丝体。通过正交实验和感官评定,调配出金针菇增智发酵保健饮料的最佳配方为：苹果酸0．08％,白砂糖8％,柠檬酸0．2％,果胶0．1％,CMC0．2％。  相似文献   

The microbiological and chemical composition of silage over the course of fermentation in round bales relative to that of silage made from unchopped and precision-chopped herbage in laboratory silos     

J. McEniry†  P. O'Kiely  N. J. W. Clipson†  P. D. Forristal‡  E. M. Doyle† 《Grass and Forage Science》2008,63(3):407-420

The composition of baled silage frequently differs from that of comparable conventional silage. A factorial experiment was conducted with three wilting treatments (0, 24 or 48 h) × three ensiling systems [unchopped grass in bales, unchopped grass in laboratory silos (LS), precision-chopped grass in LS] × six stages of ensiling to (i) confirm that the fermentation of unchopped grass in LS could be used as an adequate model for baled silage fermentation, (ii) quantify the differences between baled silage and silage made from precision-chopped herbage across a range of dry-matter contents and (c) quantify the fermentation dynamics within the various treatments. The onset of fermentation as evidenced by the accumulation of fermentation products and the decline in pH were slower ( P  < 0·05) in baled silage compared with silage made from precision-chopped herbage. Furthermore the pH ( P  < 0·001) and overall concentration of fermentation acids ( P  < 0·01) were lower while ammonia-N concentration was generally higher in baled silage, making it more conducive to the activities of Clostridia , Enterobacteria and yeast. Numbers of Enterobacteria were higher ( P  < 0·001) in baled silage in the early stages of ensilage and persisted in baled silage at the end of the storage period. The implications of a slower onset of fermentation in baled herbage are greater in farm practice, as the fermentation would be further restricted by a more extensive wilting of the herbage prior to ensiling.  相似文献   

马铃薯用作啤酒新辅料的发酵特性研究     

孙晔  张赟彬 《农产品加工．学刊》2008,(7)

研究了以马铃薯为辅料的麦汁中酵母的发酵特性,以及所得啤酒的品质,探索将马铃薯用作啤酒新辅料的可行性。结果表明,与大米为辅料的麦汁发酵特性相比,以马铃薯为辅料的完成主发酵的所需时间、在主发酵阶段可发酵浸出物含量的变化、酵母利用还原糖和α-氨基氮的情况,以及酒精生成量的变化等方面均无明显区别;双乙酰的生成情况略有不同,所得啤酒的各项理化指标均符合国家标准GB4927—2001,但氨基酸总量和必需氨基酸所占的比例,都远高于添加大米辅料酿制的啤酒。  相似文献   

金龟子绿僵菌代谢产物成分预试验及其生物碱的获得     

孔平  李倩  李永娟  唐啸华  杨鸣琦 《湖北农业科学》2008,47(2):226-228

参照植物化学成分系统预试的方法对金龟子绿僵茵发酵代谢产物进行了初步分析;在pH值≥10的条件下,采用萃取技术、TLC和紫外分光光度法对代谢产物中的总生物碱进行了提取、分离和鉴定.结果表明,代谢产物中苦马豆素的特征显色反应明显;TLC、紫外分光光度法检测发现升华后的产物中以苦马豆素为主要成分,为苦马豆素获取途径的优化提供了新思路.  相似文献   

药用植物提取物对绵羊采食量、养分消化及瘤胃发酵性能的影响     

周敏  孔雪旺  刘艳美  卢慧芳 《中国饲料》2022,1(4):33-36

文章旨在探讨日粮添加不同水平药用植物提取物对绵羊采食量、采食行为、养分消化及瘤胃发酵性能的影响.试验将60头平均体重为82.76 kg的绵羊随机分为3组,每组5个重复,每个重复4头.3组绵羊在10?w的试验期中分别饲喂基础日粮+0、80和160?mg/kg药物复合植物提取物.结果:80?mg/kg植物提取物组绵羊的平均...  相似文献   

雪茄茄衣深度发酵过程中挥发性成分的变化     

刘峰峰  方欣  李林林  谭再钰  叶明樵  朱婷婷  黄友谊  潘勇 《广东农业科学》2022,49(2):158-164

[目的]目前尚不明确深度发酵对雪茄烟叶品质的影响,而挥发性成分是影响雪茄感官品质的重要因素,为探究茄衣深度发酵过程中挥发性成分的变化,分析深度发酵对雪茄烟叶品质的影响.[方法]采用HS-SPME/GC-MS法对工业生产中雪茄茄衣深度发酵过程5个阶段的样品挥发性成分进行分析.[结果]从茄衣样品中鉴定出37种挥发性成分,在...  相似文献   

根结线虫拮抗放线菌菌株DA07118的筛选与鉴定及其发酵条件的优化   总被引：1，自引：0，他引：1  

曾庆飞  李传浩  黄惠琴  方哲  鲍时翔 《中国生物防治》2009,25(3):255-259

从海南东寨港红树林、尖峰岭等原始森林及黄流地区发病蕉园采集土壤样品,分离得到215株放线菌菌株。以根结线虫为靶标线虫对215株放线菌进行筛选,获得18株对根结线虫校正死亡率在80%以上的菌株。菌株DA07118抗线虫活性最强,校正死亡率达100%;经5次继代培养,仍具有稳定产生杀线虫活性物质的能力。通过形态学、生理生化反应和16S rDAN分类鉴定,将该菌株归类为链霉菌属Streptomyces。进一步的发酵条件优化试验,确定该菌株产活性物质的最佳培养基配方为（g/L）：麦芽糖10g、蔗糖25g、酵母膏20g、蛋白胨20g、K2HPO4 0.5g,初始pH7.0。发酵优化后,菌株发酵液的10倍稀释液处理后24h对根结线虫的校正击倒率从优化前的75.0%提高到93.7%。  相似文献   

壳聚糖对Bt发酵液的絮凝作用     

张明政  刘波  林乃铨  阮传清  蓝江林 《中国生物防治》2009,25(2):133-137

为了将Bt发酵液高效浓缩,采用壳聚糖对Bt菌株LSZ9408发酵液进行絮凝,测定了pH值、温度、壳聚糖浓度、磁力搅拌的速度和时间对壳聚糖絮凝效果的影响。结果显示,当pH值为5.0～6.0,温度30～35℃,壳聚糖浓度为0.025%,磁力搅拌器550r/min搅拌5min后,壳聚糖作为LSZ9408菌株发酵液的絮凝剂,可除去发酵液中的大部分水及有色杂质,使发酵液中的杀虫蛋白质晶体和芽孢得到很好的浓缩。  相似文献   

Fermentation Performance and Characterization of Cold-Adapted Lipase Produced with Pseudomonas Lip35     

YU Hong-wei HAN Jun LI Ning QIE Xiao-sha JIAYing-min 《中国农业科学(英文版)》2009,8(8):956-962

Strain of Pseudomonas Lip35 producing lipase was isolated in a refrigerator. Lipase production and characterization of this strain were investigated under different conditions. The Pseudomonas was cultivated in shaking flasks in a fermentation medium in various nutritional and physical environments. Lipase production has been influenced by the presence of yeast-extract, soybean powder, NaCI, and Tween-80. Maximum lipase productivity was obtained when the physical environment of the fermentation medium was optimal for 67 h. The production of lipase reached 58.9 U·mL^-1. The lipase of Pseudomonas Lip35 can be considered to be inducible, but the inducer had little influence on the production of lipase. The lipase was characterized and showed high lipolytic activity from pH 7.5-8.0. The optimum temperature was observed at 20℃ and the thermal inactivation of lipase was obvious at 60℃. The lipase activity was inhibited by K＋, stimulated by Ca^2＋, and thermostability decreased in the presence of Ca^2＋, therefore the lipase was Ca^2＋ -dependent cold-adapted enzyme.  相似文献   

黑曲霉发酵玉米秸秆产纤维素酶的研究     

张福元  宋燕青  程文晓  乔君毅 《山西农业大学学报(自然科学版)》2009,29(3)

为了优化霉菌产酶条件,获得较可靠的酶活性测定结果,采用两种酶活性测定方法,选用黑曲霉菌株,在配料中添加磷酸二氢钾等单因素试验的基础上,又进行了复因素试验,选出黑曲霉产纤维素酶的最佳添加物组合,最后测定发酵物的主要营养成分。结果表明,玉米秸秆中加入1.8倍的水,添加磷酸二氢钾0.25%、硫酸铵1.00%~1.25%、蔗糖0.50%,可以使黑曲霉菌株产CMCase、FPase酶活性达到最高;玉米秸秆经黑曲霉菌株发酵后粗蛋白提高了1.2倍,而粗纤维、中性洗涤纤维、酸性洗涤纤维分别下降了34%、19%和23%左右;利用CMCase、FPase酶活性两种方法评价黑曲霉菌株产纤维素酶的能力是可行的,整个实验中从CMCase法和FPase法得出的结论基本一致,且FPase法操作步骤简单,成本低,较CMCase法精确度高。  相似文献