水貂取皮期与配种期睾丸形态参数测定及组织学观察     

宋兴超 《特产研究》2014,(1):5-9

为探明水貂睾丸的季节性变化规律,试验测定了取皮期和配种期雄性水貂睾丸的基础形态参数,通过石蜡切片、苏木精-伊红染色方法对取皮期和配种期水貂的睾丸及附睾进行了组织学结构特征比较。结果表明,水貂取皮期睾丸呈长卵圆形,平均长径、短径和厚径分别为16.88mm±1.52mm、10.90mm±0.85mm和10.28mm±1.03mm,体积为1.05cm3±0.23cm3,平均重量达到1.032 3g±0.263 3g;水貂配种期睾丸呈短卵圆形,各项形态生理参数、体积及重量极显著高于取皮期(P0.01)。在光镜下,与取皮期相比,配种期水貂睾丸生精小管内细胞层数和种类较多,包括精原细胞、初级精母细胞、次级精母细胞和精子细胞,生精小管与附睾管管腔中存在大量精子。而取皮期生精小管内细胞疏松,仅见少量精原细胞,并且配种期生精小管直径极显著高于取皮期(P0.01)。  相似文献   

非洲鸵鸟食管组织学观察   总被引：9，自引：1，他引：9  

肖传斌  刘忠虎  梁宏德  党静  高春生  王平利 《河南农业大学学报》2005,39(1):102-105

应用常规石蜡切片,H—E染色,对非洲鸵鸟食管组织结构进行观察并与家禽和哺乳动物的食管进行比较．结果表明：非洲鸵鸟食管黏膜上皮为复层扁平上皮,但角质化不明显,固有膜内食管腺丰富,由腺细胞围成的管泡状腺直接开口于黏膜上皮,分泌大量黏液．食管肌层非常发达,分为内环肌、中纵肌、外环肌3层,环肌很厚,与纵肌的比例约为3：1、外膜是一层薄的纤维膜,内含丰富的血管．淋巴组织和神经.  相似文献   

《鱼类组织胚胎学》课程教学的几点体会     

赫晓燕 《现代农业科学》2009,(6):298-300

《鱼类组织胚胎学》是水产养殖专业必修的专业基础课。笔者根据《鱼类组织胚胎学》课程的特征,结合自身教学实践体会,对《鱼类组织胚胎学》教学改革进行了探索和实践,并对几年来在该教学实践中所使用的主要教学方法进行了总结。  相似文献   

杜仲提取物对肉仔鸡免疫器官发育影响的组织学观察     

王正朝  张正红  殷定忠  卢立志  蒋永清 《动物医学进展》2007,28(12):36-38

在饲喂基础日粮的基础上,在肉用仔鸡饮用水中添加0、1.25%、2.5%和5%的杜仲提取物,通过观察1、2、3、4、5、6周肉仔鸡法氏囊、胸腺和脾脏发育的组织学情况,来研究杜仲的生物学功能,旨在为杜仲提取物作为饲料添加剂提供理论依据.结果表明,添加2.5%、5%杜仲提取物试验组中胸腺小叶、脾小结和法氏囊小结发育程度与对照组相比均发生显著性变化,因而从组织学角度进一步表明了杜仲能够不同程度地促进免疫器官的发育,增强机体的免疫应答能力,从而提高了机体的抵抗力.  相似文献   

The effect of dietary supplementation of transcarpathian zeolite on intestinal morphology in female broiler chickens     

《The Journal of Applied Poultry Research》2017,26(3):421-430

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Dermatophilus congolensis Infection in Goats in Tanzania     

Msami HM  Khaschabi D  Schöpf K  Kapaga AM  Shibahara T 《Tropical animal health and production》2001,33(5):367-377

When goats in Eastern Tanzania were screened for skin diseases, Dermatophilus congolensis was isolated from the skin lesions in 8 of 484 animals examined. In one severely affected case, the disease was also characterized by histological studies (Gram stain, Giemsa stain and routine HE studies) and electron microscopy. The histological picture was characterized by hyperkeratosis, parakeratosis, acanthosis, folliculitis and an inflammatory cellular reaction involving the epidermis. Gram stain and Giemsa stain revealed longitudinal and transverse branching filaments in the deeper layers of the epidermis. Ultrastructural studies demonstrated D. congolensis in various morphological forms, ranging from filamentous to tuber-shaped structures, mixed with numerous coccoid bodies of variable size. In some instances, the organisms were geometrically arranged in parallel rows of beading and were present in and among the degenerated epithelial cells. Several host cells showed degenerative changes. Ticks present on the goats were Amblyomma variegatum, Rhipicephalus evertsi, Rhipicephalus pravus and Boophilus sp. The clinical signs, pathological lesions, diagnosis, epidemiology and pathogenesis of the disease are discussed.  相似文献   

Comparison of nuclear morphometric parameters in cytologic smears and histologic sections of spontaneous canine tumors     

De Vico G  Sfacteria A  Maiolino P  Mazzullo G 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2002,31(1):16-18

Background — Nuclear morphometry may provide useful diagnostic and prognostic information for neoplasms in animals. Most available data have been obtained from histologic sections. Nuclear morphometry of cytologic smears may provide important pre-operative information.
Objectives — The goal of this study was to compare nuclear morphometric parameters in cytologic smears and histologic sections from spontaneous canine tumors.
Methods — Mean nuclear area (MNA), mean nuclear perimeter (MNP), mean nuclear form factor (FF; nuclear perimeter²/4π nuclear area) and their respective SDs were assessed by image analysis of both hematoxylin and eosin-stained histologic sections and May-Grünwald-Giemsa-stained cytologic smears from the same case in 20 spontaneous canine tumors of different histogenesis. The above parameters were selected as being the best morphometric tools for measuring variation in shape and size in cells after neoplastic transformation. Data were compared by ANOVA with P<.01 considered significant.
Results — There was a significant difference between histologic and cytologic specimens for MNA, MNP, and their SDs. Only the differences between FF and the SD of FF were not statistically significant.
Conclusions — Only nuclear morphometric data related to nuclear shape and nuclear shape variability are comparable between histologic and cytologic specimens. Nuclear area and perimeter may be affected by the different fixation and smear preparation techniques used in histology and cytology.  相似文献   

Lung carcinosarcoma in a dog: gross and microscopic examination     

Salas G  Román O  Gutiérrez Díaz-Ceballos ME  Constantino F 《Veterinary journal (London, England : 1997)》2002,163(3):137-334

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Sensitivity and specificity of two serological tests for the detection of ovine paratuberculosis     

Hope AF  Kluver PF  Jones SL  Condron RJ 《Australian veterinary journal》2000,78(12):850-856

OBJECTIVE: To determine the sensitivity and specificity of an absorbed ELISA and an AGID test for the detection of clinical and subclinical paratuberculosis in sheep. DESIGN: By testing a panel of sera from 1257 Australian Merino and crossbred sheep greater than 1 year of age, of which 1137 sheep were not infected with Mycobacterium avium subsp paratuberculosis and 120 sheep had paratuberculosis. PROCEDURE: Sera were collected from 457 sheep in Victoria and 800 sheep in Western Australia. Presence of M a paratuberculosis infection in Victorian sheep was determined by histological examination of intestinal tissues, whereas sheep from Western Australia were presumed to be free of Johne's disease. The ability of an absorbed ELISA to discriminate between infected and uninfected sheep was described by test sensitivity and specificity, the distribution of ELISA OD, and the area under a receiver operating characteristic curve. RESULTS: The absorbed ELISA had a specificity of 98.2 to 99.5% (CI) and a sensitivity of 35 to 54% (CI). In sheep from infected flocks in Victoria, the AGID test had a specificity of 99 to 100% (CI) and a sensitivity of 38 to 56% (CI). The sensitivity of serological tests was higher in sheep with a body condition representative of the lower quintile of their flock of origin. CONCLUSION: The AGID test and absorbed ELISA are useful tests for the detection of ovine paratuberculosis. Although the tests had a similar accuracy, they detected different subpopulations of infected sheep with only moderate overlap. The AGID test had a higher specificity than the absorbed ELISA.  相似文献   

An Apparent Outbreak of Cutaneous Papillomatosis in Merino Sheep in Patagonia,Argentina     

Uzal FA  Latorraca A  Ghoddusi M  Horn M  Adamson M  Kelly WR  Schenkel R 《Veterinary research communications》2000,24(3):197-202

A retrospective study was performed on skin samples from an outbreak of cutaneous papillomatosis in Merino sheep that occurred in 1995. The samples were processed for routine histology, electron microscopy and immunocytochemistry for papilloma viruses. Particles of approximately 55 nm diameter were found in some nuclei of the stratum granulosum cells, while immunocytochemistry gave positive staining of cell nuclei in this layer. This study confirms that papillomas associated with papillomaviruses occur in sheep in Patagonia.  相似文献