茄科植物内生细菌的分离及拮抗菌的筛选   总被引：4，自引：0，他引：4  

李庚花  杨民和  蒋军喜  罗友强  曾敬富 《江西植保》2005,28(1):10-11

从番茄和辣椒植株中共分离到9株对植物病原真菌具有拈抗作用的内生细菌。其中，来自樱桃番茄的内生细菌株系BS-1和湘研辣椒的内生细菌株系BS-4对辣椒根腐病菌等具有较强的拈抗作用，两者在植株体内均可以定殖。BS-1和BS-4均属于芽孢杆菌。  相似文献   

利用菜青虫细胞检测几种有机溶剂和有机磷农药的毒力   总被引：5，自引：0，他引：5  

杨红  周青春  王家坤  洪华珠  陈曲侯 《植物保护学报》1996,23(1):79-83

用MTT法研究了5种有机溶剂对菜青虫细胞生长的影响及3种有机磷农药对细胞的毒力。并用微量点滴法测定了3种农药对菜青虫3龄幼虫的毒力。结果表明,5种有机溶剂除二甲苯外,其余4种二甲亚砜、乙醇、丙酮、乙酸乙酯对菜青虫细胞低浓度处理时均无很大毒性。1％浓度处理16小时后细胞存活率仍分别可达99.8％、98％、97.2％、91％。分别用菜青虫细胞测得3种有机磷农药的LC_(50)为:甲基对硫磷,106μs/ml;克线磷,147μg/ml;水胺硫磷,183μg/ml。用微量点滴法测得3种有机磷农药对菜青虫3龄幼虫的LD_(50)分别为:甲基对硫磷,0.458μg/头,克线磷,45.012μg/头;水胺硫磷,0.505μg/头。  相似文献   

Production and evaluation of monoclonal antibodies for the detection of beet mild yellowing luteovirus and related strains     

H. G. Smith  I. Barker  G. Brewer  M. Stevens  P. B. Hallsworth 《European journal of plant pathology / European Foundation for Plant Pathology》1996,102(2):163-169

A sugar-beet-infecting isolate of beet mild yellowing luteovirus (BMYV), and aBrassica-infecting isolate of beet western yellows luteovirus (BWYV) were used to produce monoclonal antibodies for epidemiological studies with BMYV and related field strains. Thirty-four monoclonal antibodies were tested for their reaction with 9 luteoviruses in triple-antibody-sandwich enzyme-linked immunosorbent assay. One (MAFF 24) is now routinely used in the UK for detecting BMYV and BWYV in plants and aphids, although it does not discriminate between them. Heterologous reactions were detected between some of the monoclonal antibodies and potato leafroll virus (PLRV), bean leafroll virus (BLRV) and barley yellow dwarf virus (BYDV-RPV). 38% of antibodies raised to BWYV reacted with PLRV compared with 4% of those raised to BMYV. Monoclonal antibodies were produced which distinguished a sugar-beet-infecting isolate of BMYV with differing host range and serological properties from the commonly-occurring field strain.  相似文献   

A screening test for Mycosphaerella brassicicola on Brassica oleracea     

J. E. Van Den Ende 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(4):227-236

A greenhouse screening method for resistance to ringspot (Mycosphaerella brassicicola) inBrassica oleracea is described. High infection levels were achieved by spraying young plants by mycelial inoculum enriched with 3% sucrose. The screening method was tested on three Brussels sprouts, three cabbage and three cauliflower cultivars, with known reactions to ringspot in the field. Resistance was expressed both in cotyledons and true leaves by a lower number of lesions than the susceptible control and/or by hypersensitive reactions. Results of the seedling tests reflected differences in resistance in the field. Under controlled conditions the new test can be applied year-round to young plants, thus accelerating selection procedures.  相似文献   

Impedimetric evaluation of compounds for antifungal activity versus Aspergillus niger     

Stephen D. Bryant 《Pest management science》1995,45(3):257-262

The procedure described utilized a microbial monitoring system (bactometer) to measure impedimetrically the inhibitory activity of known antifungal compounds (2-(thiocyanomethylthio)benzothiazole [TCMTB], 3-iodo-2-propynylbutyl carbamate [IPBC], chlorothalonil, tebuconazole, and copper-8-quinolinolate [Cu-8]) on growth of Aspergillus niger Teigh. in a liquid medium. The MIC values determined were based on the activity of the compounds to inhibit a change in the capacitance of the medium during a 96-h period after inoculation. The MIC values obtained with the bactometer were compared with MIC values derived from the inhibition of radial growth of A. niger on the same liquid medium solidified with agar. The MIC values for all the compounds tested were substantially lower when determined with the bactometer than on agar. The impedimetric evaluation of compounds for antifungal activity offers advantages of speed, sensitivity, and data collection.  相似文献   

Comparison of antibody values in sera of pigs vaccinated with a subunit or an attenuated vaccine against classical swine fever     

Terzić S  Jemersić L  Lojkić M  Madić J  Grom J  Toplak I  Sver L  Valpotić I 《Veterinary research communications》2003,27(4):329-339

Ten pigs, aged 85 days, were vaccinated with a subunit vaccine containing 32 g of classical swine fever virus glycoprotein E2 (gp E2) (group 1), and a further 10 pigs were vaccinated with a C strain vaccine (10^4±0.15 TCID₅₀/ml), produced by amplification in minipig kidney (MPK) cell culture (group 2). Nine non-vaccinated pigs served as a control group (group 3). Serum samples were collected before (day 0) and at 4, 10, 21 and 28 days after vaccination and were analysed by two commercially available enzyme immunoassays and by a neutralizing peroxidase-linked assay (NPLA). At the same times, peripheral blood was taken for determining the total leukocyte count and the body temperature was taken daily. Antibodies were not detected in serum samples collected before vaccination (day 0), and no side-effects that could be connected with vaccination were observed during the trial. Ten days after vaccination 6/10 pigs vaccinated with the subunit vaccine were seropositive. On days 21 and 28, the ratios of serologically positive to vaccinated pigs were 9/10 and 10/10, respectively. Four of the ten pigs that were vaccinated with the C strain vaccine were positive on day 21 and 9/10 on day 28. However, the results of the NPLA showed that only 4/10 pigs had an antibody titre >1:32 at the end of the trial in both the vaccinated groups, even though the subunit vaccine initiated an earlier and higher level of neutralizing antibodies than the vaccine produced from the C strain. Challenge was performed 28 days after vaccination on four randomly selected pigs from both vaccinated groups. The pigs survived the challenge without showing any clinical signs of classical swine fever (CSF), while two nonvaccinated control pigs died on the 10th and 12th days after infection.  相似文献   

Canine leukocyte adhesion deficiency: presence of the Cys36Ser beta-2 integrin mutation in an affected US Irish Setter cross-breed dog and in US Irish Red and White Setters     

Foureman P  Whiteley M  Giger U 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(5):518-523

Canine leukocyte adhesion deficiency (CLAD) is a primary immunodeficiency disease characterized by recurrent bacterial infections in the presence of marked leukocytosis. The disease was 1st described in the mid-1980s in a cross-breed Irish Setter Dog in the United States. It results from a defective beta-2 subunit of heterodimeric leukocyte adhesion proteins. The causative mutation for CLAD in Irish Setter Dogs from Europe has been identified as a missense mutation at base pair position 107 in the beta-2 integrin subunit gene (ITGB2) that results in an amino acid change from cysteine to serine at amino acid 36 (Cys36Ser) in the beta-2 integrin subunit protein. In the current work, the originally described dog with CLAD has been genetically tested and shown to have the same mutation as the European Irish Setters. This suggests that the mutation has been in the Irish Setter population for many generations spanning more than 2 decades. A related breed, the Irish Red and White Setter, has a history of interbreeding with Irish Setters and shares a common ancestry with the Irish Setter breed. DNA from Irish Red and White Setters residing in the United States was screened either by sequencing or by the newly developed restriction enzyme test for the Irish Setter Cys36Ser CLAD mutation. Seven of 54 dogs tested (13%) were found to be carriers of the Irish Setter CLAD mutation. Five of these were directly related to a sire from the UK, demonstrating the importation of an allele from another continent and establishing the need for genetic testing in this breed in the United States.  相似文献   

In vitro analysis of antiangiogenic activity of fungi isolated from clinical cases of equine keratomycosis     

Welch PM  Gabal M  Betts DM  Whelan NC  Studer ME 《Veterinary ophthalmology》2000,3(2-3):145-151

OBJECTIVE: The goal of this project was to explore the possibility that fungal organisms produce metabolites that inhibit angiogenesis. Procedures Fungal cultures were obtained from cases of keratomycosis, grown in Sabouraud's dextrose broth, and sterile filtered for use in experiments. The Matrigel assay was used to screen the filtrate samples for antiangiogenic activity. Matrigel is a basement membrane matrix that supports the differentiation of human umbilical vein endothelial (HUVE) cells into a capillary-like network of tubules. HUVE cells were cultured using standard techniques and passaged at confluence, with all cells being used at passage 3-6. HUVE cells (40 000 cells) were pipetted into each well of a 24-well tissue-culture plate coated with Matrigel. An aliquot of fungal media filtrate was added to each well and the plates allowed to incubate for 18 h, at which time they were evaluated for tubule formation. RESULTS: Two fungal isolates showed inhibition of tubule formation. The addition of 100, 200 and 400 &mgr;L of the fungal media filtrate from the first isolate (Fusarium sp. 99A34574) produced a consistent and dose-dependent inhibition of tubule formation. The second isolate (Aspergillus sp. 271599) did not show inhibition of tubule formation with 100 or 200 &mgr;L added to the wells, however, it did show inhibition at 400 &mgr;L/well. The remaining three isolates did not cause inhibition at any concentration. CONCLUSIONS: Our findings suggest that certain fungal organisms produce metabolites that inhibit tubule formation in vitro, and that these metabolites may play a significant role in altering the host vascular response to fungal infections of the cornea.  相似文献   

鸡肝脏和肌肉组织中常山酮残留的ELISA检测   总被引：3，自引：0，他引：3  

吴宁鹏  杜向党  沈建忠 《畜牧兽医学报》2006,37(6):597-602

将常山酮进行人工改造,制备了半抗原常山酮琥珀酸衍生物（Hal-suc）.采用N-羟基琥珀酰亚胺活性酯法将半抗原与牛血清白蛋白（BSA）、卵清白蛋白（OVA）偶联,制备免疫原和包被原.动物免疫6次后采血制备抗血清,以间接ELISA法测定血清效价,测得抗血清的最佳工作浓度为1∶102 400.建立了常山酮在鸡肝脏和肌肉组织中检测的ELISA法,该方法在50、100、500 ng/g 的添加浓度水平下,在鸡肝脏和肌肉组织中测得的平均回收率范围分别为74.2%～96.8%和74.3%～90.0%,检测限分别为28和19 ng/g.  相似文献   

传统发酵酸马奶中瑞士乳杆菌耐酸性的筛选   总被引：2，自引：0，他引：2  

李海燕  徐杰  云月英  邵亚东  孟和毕力格  张和平 《乳业科学与技术》2006,28(4):158-160

从新疆地区牧民家庭32份自然发酵酸马奶样品中分离鉴定出的82株瑞士乳杆菌中，通过耐酸实验选出39株可在pH3．0和pH3．5酸性环境中生长的菌株进行pH为3．0的人工胃液的耐受性实验。结果表明不同菌株对人工胃液的耐受能力有很大差异。  相似文献