Development of STS markers and QTL validation for common bacterial blight resistance in common bean   总被引：1，自引：0，他引：1  

S. Liu    K. Yu    S. J. Park 《Plant Breeding》2008,127(1):62-68

Common bacterial blight (CBB) of common bean ( Phaseolus vulgaris L.), is one of the major diseases that decrease yield and quality. A major quantitative trait locus (QTL) for CBB resistance from line XAN 159 was transferred into two bean lines, HR45 and HR67. Previous studies identified that two markers are linked to this QTL but the chromosome location was not consistent. To identify more tightly linked markers and to verify the chromosome location, 65 additional markers were mapped using 81 recombinant inbred lines (RILs) derived from a cross HR67 × OAC95-4. The QTL was mapped to a 13 cM region on chromosome 1 and defined by eight molecular markers that explained 25–52% of the phenotypic variation. Six tightly linked amplified fragment length polymorphism markers (0.6–9.7 cM from the QTL peak) were converted into seven sequence tagged site markers, three of which were mapped to this QTL. Five tightly linked markers were used to screen 907 F₂ plants derived from a cross HR45 × 'OAC Rex' and four of them were linked to each other within 4.2 cM. These markers may be useful in marker-assisted selection and map-based cloning of this major QTL.  相似文献   

Identification of Multiple Alleles at the Wx Locus and Development of Single Segment Substitution Lines for the Alleles in Rice   总被引：1，自引：1，他引：0  

ZENG Rui-zhen ZHANG Ze-min HE Feng-hua XI Zhang-ying Akshay TALUKDAR SHI Jun-qiong QIN Li-jun HUANG Chao-feng ZHANG Gui-quan 《水稻科学》2006,13(1):9-14

Amylose content (AC) is a key determinant of the cooking and processing quality of rice (Oryza sativa). It has been reported that the amylose content is mainly controlled by the Wx locus [1]. By using RFLP analysis, two alleles, largely corresponding to t…  相似文献   

胚乳外观标记与RVA谱理化指标相结合辅助改良早籼稻食用品质   总被引：7，自引：0，他引：7  

吴殿星  舒庆尧  夏英武 《中国水稻科学》2002,16(1):80-82

对具有较低和中等表观直链淀粉含量的浙辐504和IR64的杂交后代进行观察,发现胚乳外观存在4种类型：乳白色、云雾状、透明状及各外观混合体。与胚乳外观紧密关联,表观直链淀粉含量(AAC)也相应有4种含量水平：明显低AAC变异型、浙辐504型、IR64型和介于双亲的中间型。RVA谱分析表明,不同胚乳外观的淀粉粘滞特性差异明显。透明状外观尽管AAC相仿,但个体间淀粉粘滞性分化明显,只有个别优良品系的RVA谱与优质亲本IR64相似。表明以胚乳外观标记和RVA谱理化指标辅助选择可有效控制表观直链淀粉含量和淀粉粘滞性,是改良早籼稻米品质的一条有效途径。  相似文献   

Selection on multiple QTL with control of gene diversity and inbreeding for long-term benefit     

Y Li  H N Kadarmideen  J C M Dekkers 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2008,125(5):320-329

The purpose of this study was to develop and investigate selection strategies that aim at maximizing long-term genetic response while conserving gene diversity and controlling inbreeding in populations of limited effective size, assuming complete knowledge of all genes affecting a quantitative trait. Three selection strategies were proposed to select on 100 quantitative trait loci (QTL) and compared with truncation selection on breeding value. Alternative selection strategies aimed at maximizing the average breeding value of parents with a penalty on (1) the number of unfavourable QTL genotypes among parents (OS-I), (2) the negative of the logarithm of the frequency of the favourable allele at each QTL among parents (OS-II), and (3) the average pedigree relationship among parents (OS-III). When all QTL and their effects were known, the strategies examined were able to obtain extra long-term responses, conserve QTL diversity and reduce inbreeding, compared with truncation selection. Strategy OS-II was the most effective in conserving QTL diversity and OS-III in reducing inbreeding. By changing the magnitude of the penalties applied, the impact on long-term response, inbreeding and diversity can be controlled. Extra long-term responses over truncation selection of OS-I and OS-II were even greater when effects of QTL were estimated rather than assumed known, indicating the applicability of results to practical strategies for marker-assisted selection. Extra responses are expected to be reduced for larger population sizes.  相似文献   

Unraveling waterlogging tolerance-related traits with QTL analysis in reciprocal intervarietal introgression lines using genotyping by sequencing in rapeseed(Brassica napus L.)          下载免费PDF全文

DING Xiao-yu  XU Jin-song  HUANG He  QIAO Xing  SHEN Ming-zhen  CHENG Yong  ZHANG Xue-kun 《农业科学学报》2020,19(8):1974-1983

  相似文献   

分子标记技术在绵羊育种中的应用现状分析     

郭应威  孙珂欣  田晨  曹睿  赵海东  王淑辉  孙秀柱 《中国畜牧兽医》2018,45(12):3505-3512

分子标记是以个体间核苷酸序列变异为基础的遗传标记,是DNA水平上的遗传多样性的直接反应。分子标记技术是在DNA水平上进行多态性分析的一种技术手段,具有效率高、准确度高的特点,在绵羊育种中有着广泛的应用。分子标记技术不仅可以对绵羊的基因进行定位,而且可以对绵羊群的遗传结构进行分析,重要的是可以进行绵羊的标记辅助育种,对绵羊的育种起重要作用。作者介绍了以Southern杂交、PCR扩增、重复序列和单核苷酸多态性（SNP）为基础的分子标记技术的基本原理及优缺点,重点介绍了这些分子标记技术在绵羊的体尺、屠宰、繁殖等性状中进行标记辅助选择时的应用,揭示了在实际生产中分子标记技术对于绵羊选种与选配、提高其经济价值的重要意义,并基于目前分子标记技术在绵羊育种中的运用,以及未来分子标记技术的应用作出展望。  相似文献   

节水抗旱稻恢复系的抗褐飞虱分子标记辅助选育及抗性评价     

ZHANG An-Ning  LIU Yi  WANG Fei-Ming  XIE Yue-Wen  KONG De-Yan  NIE Yuan-Yuan  ZHANG Fen-Yun  BI Jun-Guo  YU Xin-Qiao  LIU Guo-Lan  LUO Li-Jun 《作物学报》2019,45(11):1764-1769

褐飞虱是水稻的主要害虫之一,利用水稻抗褐飞虱基因培育抗虫品种是目前公认最经济有效、环境友好的策略。本研究利用水稻功能基因组已克隆的抗褐飞虱基因,通过分子标记辅助选择和常规回交育种相结合的方法,将抗褐飞虱基因Bph6、Bph9、Bph14和Bph15单独和聚合导入到节水抗旱稻恢复系旱恢3号,获得了一系列含有单基因、双基因、三基因和四基因的改良系。采用标准苗期集团筛选法进行褐飞虱抗性鉴定,评价这些基因在旱恢3号背景下的效应及相互作用。表明单基因改良系中, Bph9的抗性最强,且Bph9 Bph6 Bph15 Bph14;在聚合改良系中,抗性均优于单基因改良系,四基因聚合改良系的抗性最强,不同基因型组合的抗性效应是Bph6+Bph9+Bph14+Bph15Bph6+Bph9 Bph6+Bph9+Bph14 Bph6+Bph9+Bph15 Bph6+Bph14+Bph15 Bph9+Bph14+Bph15 Bph14+Bph15。在自然条件下,改良系与旱恢3号在株高、有效穗和千粒重等农艺性状上差异不显著,其他性状与旱恢3号相仿或略差。本试验表明单独和聚合导入Bph6、Bph9、Bph14和Bph15基因能显著提高节水抗旱稻恢复系的褐飞虱抗性,这4个基因的加性效应明显,可为今后节水抗旱稻抗褐飞虱育种提供理论依据和材料基础。  相似文献   

番茄分子育种现状与展望——从基因克隆到品种改良   总被引：1，自引：0，他引：1  

杜敏敏  周明  邓磊  李传友  李常保 《园艺学报》2017,44(3):581-600

在界定分子育种概念的基础上,综述了近30年番茄主要农艺性状相关基因克隆及其调控的研究进展,简要分析了番茄分子育种研究现状,初步探讨了未来中国番茄分子育种的发展策略。  相似文献   

Transfer of the Rsv3 locus from ‘Harosoy’ for resistance to soybean mosaic virus strains C and D in Japan     

Shin Kato  Yoshitake Takada  Satoshi Shimamura  Kaori Hirata  Takashi Sayama  Fumio Taguchi-Shiobara  Masao Ishimoto  Akio Kikuchi  Takeshi Nishio 《Breeding Science》2016,66(2):319-327

Resistance to soybean mosaic virus (SMV) is imperative for soybean (Glycine max (L.) Merr.) production in the Tohoku region. Molecular markers for SMV resistance were previously reported for U.S. SMV strains, but they cannot be applied because of the differences in strain classification between Japan and the U.S. A U.S. variety ‘Harosoy’ has been used mainly as a donor of resistance to SMV strains C and D in a Japanese breeding program, resulting in resistant varieties such as ‘Fukuibuki.’ Because ‘Harosoy’ harbors the Rsv3 gene conferring resistance to the virulent SMV strain groups, G5 through G7, it appears that the Rsv3 gene confers resistance to strains C and D. In this study, we introduced resistance to the two strains from ‘Fukuibuki’ into a leading variety ‘Ohsuzu’ by recurrent backcrossing with marker-assisted selection. All lines selected with markers near Rsv3 showed resistance to the strains, suggesting that the Rsv3 locus is responsible for the resistance. Three years of trials showed that one of the breeding lines, ‘Tohoku 169,’ was equivalent to ‘Ohsuzu’ with respect to agricultural characteristics such as seed size, maturity date, and seed yield, except for the SMV resistance.  相似文献   

麻类作物分子育种的研究现状与展望   总被引：1，自引：0，他引：1  

陈美霞  祁建民  刘伟  徐建堂  祁伟  李爱青  粟建光  陶爱芬  牛小平 《福建农业学报》2012,27(7):780-786

麻类作物资源的保护和开发利用的成功依赖于对基因库资源遗传变异的数量、分布及其进化关系充分的了解和掌握。传统的研究方法有很大局限性,分子标记的出现弥补了传统方法的不足,成为现代科学研究的有利工具。目前关于麻类作物的遗传背景知识相对有限,但是对于作物育种又是必需的。一张高密度的遗传连锁图谱可以用于重要性状定位、重要基因克隆、比较基因组学研究和分子标记辅助育种。本文系统地论述了利用分子标记技术在6种麻类作物包括红麻、黄麻、亚麻、苎麻、大麻、剑麻的遗传连锁图谱构建及基因定位、分子标记辅助育种方面的研究进展。  相似文献