水稻早衰突变体els-R7954的遗传分析和初步定位     

孙出  童杰鹏  王艳  童川  沈圣泉 《核农学报》2015,29(3):421-427

为了深入研究水稻早衰机理,找到有效消除和延缓植株早衰的方法,本研究通过350Gy60Co-γ射线辐照水稻浙恢7954干种子,获得1份特异性水稻早衰突变体。观察该突变体生物学性状,发现苗期生长正常,分蘖末期叶片出现早衰现象,灌浆期整个植株枯黄,早衰现象非常明显,将其暂名为elsR7954(early leaf senescence)。遗传分析和基因定位发现,els-R7954受单隐性核基因控制,位于第2染色体SSR标记RM530和RM3774之间,距RM530约5.0c M。为进一步克隆该基因,丰富叶片早衰的分子机制奠定了一定基础,也为研究水稻早衰,最终提高产量和品质提供可能。  相似文献   

北疆早熟陆地棉芽黄突变体杂种F_1优势分析及优良组合的筛选     

周小凤  李生秀  肖光顺  董承光  马晓梅  李保成 《安徽农业科学》2015,(18)

[目的]培育适合新疆北疆棉区种植的早熟杂交棉品种,探讨杂种F1代的杂种优势,为北疆棉区提供高产优质早熟杂交棉品种。[方法]2011年以芽黄突变体为母本,配置14个杂交组合,并筛选出强优势组合。[结果]竞争优势分析结果表明:有11个参试组合子棉产量超过CK;有13个组合皮棉产量超过CK;其中组合KH1148和KH1150的早熟性好、丰产性突出且纤维品质综合性状优良。[结论]组合KH1148和KH1150可作为早熟杂交棉组合适于北疆棉区种植。株高、衣分、比强度存在正向杂种优势;以115-23和71-7为母本的组合,在农艺性状、产量等综合性状上有强的优势。  相似文献   

拟南芥核苷磷酸化酶基因的组织表达及其T-DNA插入突变体鉴定     

徐文晶  程玉祥 《贵州农业科学》2015,(1)

为探明植物核苷磷酸化酶家族基因信息及生理功能,采用PCR扩增的方法,研究了模式植物拟南芥核苷磷酸化酶家族基因在不同组织中的表达,并对其核苷磷酸化酶基因T-DNA插入突变体进行鉴定。结果表明:At4g24340和At4g28940基因在根组织中大量转录表达,其他组织均微量检测到转录表达;At4g24350在各个组织中均有少量转录表达;经T-DNA插入结合转录表达鉴定,At4g28940和At4g24350基因为敲除突变体。  相似文献   

PG,ACC,乙烯对番茄果实成熟的影响   总被引：14，自引：0，他引：14  

陆春贵  周立新 《园艺学报》1995,22(1):57-60

番茄果实在成熟过程中硬度下降迅速，ＰＧ活性急剧上升，表现出显著的负相关；用ＡＣＣ和乙烯利处理绿熟期果实，结果乙烯促进了正常番茄和ａｌｃ番茄果实成熟进程，其ＰＧ活性也升高，而外源乙烯和ＡＣＣ不影响ｎｏｒ基因突变体果实的成熟进程。ＡＣＣ和ＰＧ无直接关系。  相似文献   

甘肃菊科牧草霜霉病调查研究     

孟有儒 《草业科学》2003,20(4):40-42

经调查，甘肃12种菊科牧草上发生霜霉病，由盘霜霉属的7个种、轴霜霉属1个种和首次在甘肃发现的类霜霉属2个种侵梁所致，并发现鉴定盘霜霉新组合种和变种各1个。霜霉病一般在河西地区较陇东、陇南发病严重，有的发病率高速40％以上。  相似文献   

玉米籽粒突变体dek101的表型分析和精细定位     

Xin-Ran SONG  Shu-Ting HU  Kai ZHANG  Ze-Jin CUI  Jian-Sheng LI  Xiao-Hong YANG  Guang-Hong BAI 《作物学报》1962,46(12):1831-1838

As the storage organ of maize, kernel development and accumulation of storage production directly determines maize yield and quality. In this study, a stable defective kernel mutant, named as defective kernel 101 (dek101), was identified during the development of double haploid (DH) lines in maize. The dek101 kernels displayed severely shrunk kernel appearance, significantly reduced kernel weight, lethal embryo, defective endosperm and were incapable of germinating. The dek101 showed obvious developmental abnormalities at 12 days after pollination (DAP). The fresh weight, dry weight and volume of the kernels were no longer increased after 21 DAP. Scanning electron microscopy (SEM) observation revealed that the starch granules of dek101 were significantly smaller compared with wild-type kernels. Genetic analysis demonstrated that the mutant trait was controlled by a recessive single gene. Using 441 F₂ individuals and 1648 F₃ individuals, dek101 was narrowed down to a genomic region of about 300 kb between the InDel marker IDP2182 and IDP4600 on chromosome 1, which contains five predicted genes. These results laid the foundation for mining functional genes related to maize kernel development and deciphering the mechanism of grain development.  相似文献   

Impaired purine biosynthesis affects pathogenicity of <Emphasis Type="Italic">Fusarium oxysporum</Emphasis> f. sp. <Emphasis Type="Italic">melonis</Emphasis>     

Youlia?Denisov  Oded?Yarden  Stanley?FreemanEmail author 《European journal of plant pathology / European Foundation for Plant Pathology》2005,112(3):293-297

The vascular wilt pathogen Fusarium oxysporum f. sp. melonis causes worldwide yield losses of muskmelon. In this study, we characterized a UV-induced non-pathogenic mutant (strain 4/4) of F. oxysporum f. sp. melonis, previously identified as a potential biological control agent. During comparative analysis of vegetative growth parameters using different carbon sources, mutant strain 4/4 showed a delay in development and secretion of extracellular enzymes, compared to the wild type strain. Amendments of the growth medium with yeast extract, adenine or hypoxanthine, but not guanine, complemented the growth defect of strain 4/4, as well as secretion and partial activity of cellulases and endopolygalacturonases, indicating that the strain is an adenine auxotroph. Incubation of strain 4/4 conidia in adenine solution, prior to inoculation of muskmelon plants, partially restored pathogenicity to the mutant strain.  相似文献   

一个新的水稻短根毛突变体的遗传分析和基因定位     

丁沃娜  黄炜  宁永强  朱世华 《作物学报》2012,38(2):240-244

根毛是植物吸收水分和养分的重要器官。本研究从T-DNA突变体库中获得一个以中花11为遗传背景的水稻短根毛突变体, 命名为ossrh3 (Oryza sativa short root hair 3)。该突变体的根毛伸长严重受阻, 并且伴随株高、主根长、侧根长和侧根数目等性状的改变。遗传分析表明该突变性状受1对隐性单基因控制, 利用ossrh3纯合体和籼稻品种Kasalath杂交构建F₂定位群体, 利用已公布的水稻SSR (simple sequence repeat)和自行设计的STS (sequence- tagged site)标记, 最终将OsSRH3定位在水稻第1染色体上的标记S38978和S39016之间, 物理距离约为37.7 kb, 包含8个候选基因, 为进一步克隆OsSRH3基因和研究禾本科作物根毛发育的分子调控机理提供了依据。  相似文献   

Identification and Gene Mapping of a multi-floret spikelet 1 （mfsl） Mutant Associated with Spikelet Development in Rice     

REN  De-yong LI  Yun-feng WANG  Zeng XU  Fang-fang GUO  Shuang ZHAO  Fang-ming SANG  Xian-  chun LING  Ying-hua HE  Guang-hua 《中国农业科学(英文版)》2012,(10):1574-1579

  相似文献   

Characterization of a Novel Chlorophyll-Deficient Mutant Mt6172 in Wheat     

GUO Hui-jun  ZHAO Hong-bing  ZHAO Lin-shu  GU Jia-yu  ZHAO Shi-rong  LI Jun-hui  LIU Qing-chang  LIU Lu-xiang 《中国农业科学(英文版)》2012,11(6):888-897

Identification of new chlorophyll-deficient mutants will provide materials for studying signaling components and pathways between plastid and nucleus.A novel chlorophyll-deficient mutant,named Mt6172,w...  相似文献