青海12号蚕豆栽培技术优化研究     

刘玉皎  张小田  刘洋  李萍  侯万伟  耿贵工 《勤云标准版测试》2009,29(20)

良种良法综合配套对于农作物新品种在生产中发挥增产作用具有重要意义,青海12号蚕豆是2005年审定的超大粒蚕豆新品种,为了更好地发挥新品种的增产潜力,采用二次饱和D-最优设计,以肥料(x1)、密度(x2)与打顶期(x3)等3项蚕豆主要农艺措施为试验变量,建立青海12号蚕豆产量与主要变量的数学模型,以模型为基础,分析各因素对青海12号蚕豆产量的影响效应,并决策优化栽培方案,为丰产栽培技术规范制定提供依据.  相似文献   

Transformation of Sclerotinia sclerotiorum with the green fluorescent protein gene and fluorescence of hyphae in four inoculated hosts     

A. P. de Silva  M. D. Bolton  B. D. Nelson 《Plant pathology》2009,58(3):487-496

To obtain a genetic marker to observe and study the interaction of Sclerotinia sclerotiorum with its hosts, isolates ND30 and ND21 were transformed using pCT74 and gGFP constructs, both containing genes for the green fluorescent protein (GFP) and hygromycin B phosphotransferase. Putative transformants were obtained using polyethylene glycol-mediated transformation of protoplasts. Seven stable gfp transformants were identified and evaluated for fluorescence in vitro and in planta , pathogenicity and colonization of host tissues. Real-time quantitative polymerase chain reaction detected a single copy of the gfp gene in transformants. Fluorescence was quantified directly from mycelium and protein extracted from hyphae. The seven transformants (four from ND30 and three from ND21) were pathogenic on dry bean, canola, soybean and sunflower. However, depending on the host, three transformants differed significantly ( P  = 0·05) in the length of lesions formed compared to the wild-type. Hyphae fluoresced in plant tissue and could clearly be distinguished from plant cells. Infection and colonization of tissues were clearly visible with a fluorescent microscope. Transformants differed in the intensity of GFP expression both in vitro and in planta .  相似文献   

进境豇豆种子携带种传病毒的检测与鉴定   总被引：2，自引：0，他引：2  

陈青  林石明  陈红运  廖富荣  余芳平  陈洪俊  朱水芳 《植物病理学报》2009,39(1):91-94

 Imported cowpea seeds were detected with growing test, ELISA assay and RT-PCR method. The ELISA results showed that cowpea seedlings with symptoms reacted positively with antibody against Southern bean mosaic virus (SBMV). The 979 bp of fragment could be amplified from two positive ELISA samples using primers specific for Southern cowpea mosaic virus (SCPMV), and the sequence determination results proved that the pathogen existing in imported cowpea seeds was SCPMV. The positive ELISA results with SBMV antibody could be further confirmed by RT-PCR amplification with specific primers designed to amplify the coat protein gene and 3' noncoding region of SCPMV and SBMV. The RT-PCR method presented here was suitable for molecular identification of SBMV and SCPMV in entry-exit plant quarantine laboratories.  相似文献   

野生酸枣疯病与栽培大枣疯病发生的关系   总被引：1，自引：0，他引：1  

田国忠  徐启聪  李永  温秀军  王振亮 《植物保护学报》2009,36(6):529-536

为了明确野生酸枣疯病与栽培大枣疯病发生和流行的关系,采用随机徒步调查、挖根和接穗嫁接法对我国野生酸枣、栽培大枣及大枣接穗嫁接野生酸枣的枣疯病进行了田间调查,并取样检测病菌及比较不同菌株的保守基因序列.结果显示,我国野生酸枣疯病发生范围广,且地区间自然发病率差异很大,在0～40%之间;病株呈明显的团簇状分布,病菌在团簇中的根蘖苗与母株间传播或通过介体昆虫传播到后代种子苗上.在枣疯病流行区,栽培大枣发病与枣园周围分布的野生酸枣发病程度有关;用感病品系的接穗或带菌接穗嫁接到野生酸枣砧木上易导致嫁接苗发病和病害流行,而采用抗病的壶瓶枣和婆枣抗病品系接穗嫁接野生酸枣则发病率明显下降.用巢式PCR进行的病菌检测结果显示,在病害流行区酸枣或大枣无症状枝叶样品的带菌检出率为10%～32%.不同地区栽培大枣和野生酸枣上植原体的16S rDNA、16S-23S rDNA间区(SR)及核糖体蛋白基因(rp)序列比较鉴定结果显示,侵染酸枣的植原体与栽培大枣疯病植原体应为相互传染的同种致病菌.  相似文献   

2个菜豆品种苗期生长对外源铬的响应     

杨和连  张百俊  李贞霞  陈碧华 《贵州农业科学》2009,37(6)

采用土培法,研究了不同浓度的外源铬对2个菜豆品种幼苗生长的影响.试验表明:处理组Ⅲ(15 mg/kg),Ⅳ(25 mg/kg)的外源铬对2个菜豆品种幼苗的株高、叶面积、主根长有显著或极显著的抑制作用.各项指标综合分析表明,特选97-5菜豆比四通2号菜豆对铬敏感.  相似文献   

不同基因型蚕豆原花青素含量分析     

刘玉皎 《湖北农业科学》2009,48(10)

采用紫外分光光度法对8个不同基因型蚕豆品种原花青素含量进行测定.结果表明,蚕豆种皮原花青素含量范围在0.122%～5.444%,平均为2.784%,不同品种间差异极显著.蚕豆种皮颜色由浅变深,其原花青素含量呈近似线性增长,但种皮颜色相同的蚕豆品种之间原花青素含量差异不显著.蚕豆种皮颜色可作为鉴别原花青素含量的重要指示性状.  相似文献   

TBHQ对苦豆子总碱光分解抑制的研究     

周玲 《安徽农业科学》2009,37(36):18021-18022

[目的]研究苦豆子（Sophora alopecuroides L.）总碱光分解情况及抗氧化剂特丁基对苯二酚（TBHQ）对其光分解抑制情况。[方法]将苦豆子总碱置于太阳光及紫外光下照射,研究光分解情况。[结果]苦豆子总碱属于光易分解物质,其光分解半衰期不超过1h运用抗氧化剂TBHQ能有效抑制苦豆子总碱光分解。不同浓度TBHQ对苦豆子总碱光分解的抑制效果不同,处理浓度越大,其抑制苦豆子总碱光分解的作用越强。[结论]为寻找合适的抑制苦豆子生物碱光分解的稳定剂提供依据。  相似文献   

Screening of cultivated and wild adzuki bean for resistance to race 3 of <Emphasis Type="Italic">Cadophora gregata</Emphasis> f. sp. <Emphasis Type="Italic">adzukicola</Emphasis>, cause of brown stem rot     

Norio Kondo  Hisanori Shimada  Shohei Fujita 《Journal of General Plant Pathology》2009,75(3):181-187

Two diseases of adzuki bean, brown stem rot (BSR, caused by Cadophora gregata f. sp. adzukicola) and adzuki bean Fusarium wilt (AFW, caused by Fusarium oxysporum f. sp. adzukicola), are serious problems in Hokkaido and have been controlled using cultivars with multiple resistance. However, because a new race of BSR, designated race 3, was identified, sources of parental adzuki bean for resistance to race 3 were needed. Therefore, we examined 67 cultivars and lines of cultivated and wild adzuki bean maintained at the Tokachi Agricultural Experiment Station using a root-dip inoculation method. Consequently, nine adzuki bean cultivars, one wild adzuki bean accession and 30 lines (including two lines resistant to all the three races of BSR and AFW) were confirmed to be resistant or tolerant to race 3 of BSR, and we found a cultivar Akamame as well as a wild adzuki bean Acc2515 to be a new source for a resistance gene to the race 3. This cultivar also holds promise as a source of resistance against other races of BSR and AFW.  相似文献   

Using a fermented mixture of soybean meal and earthworm meal to replace fish meal in the diet of white shrimp,Penaeus vannamei (Boone)          下载免费PDF全文

Shieh‐Tsung Chiu  Saou‐Lien Wong  Ya‐Li Shiu  Chiu‐Hsia Chiu  Wang‐Chen Guei  Chun‐Hung Liu 《Aquaculture Research》2016,47(11):3489-3500

An experiment was conducted to evaluate the potential of a Bacillus subtilis E20‐fermented mixture (FSFEM) containing soybean meal (SBM) and Eisenia fetida earthworm meal (EM) at a ratio of 4:1 to increase the methionine level in order to satisfy the methionine requirement of white shrimp, Penaeus vannamei in a diet with fish meal (FM) completely replaced by mixtures. B. subtilis E20 fermentation improved the mixture's palatability and utilization based on better growth performance in comparison to shrimp fed FSEM (contains fermented SBM and EM at a ratio of 4:1) diets. FSFEM is a good substitute for FM. Maximal replacement levels of FM with FSFEM were 80% in a shrimp diet with 37% of crude protein and 7% of crude lipid based on weight gain and 100% based on feeding efficiency. In addition, shrimp fed experimental diets had no significant differences in survival after being challenged by Vibrio alginolyticus. It is suggested that B. subtilis E20‐FSFEM has the potential to replace FM in cultured shrimp diets.  相似文献   

肉桂酸加剧蚕豆枯萎病发生的根际微生物效应   总被引：2，自引：0，他引：2  

董艳  董坤  杨智仙  汤利  郑毅 《园艺学报》2016,43(8):1525-1536

通过盆栽和室内试验研究了肉桂酸处理对蚕豆种子萌发,幼苗生长及尖孢镰孢菌菌丝生长,蚕豆枯萎病发生,根际微生物的活性、多样性和群落结构的影响。结果表明：肉桂酸处理抑制了蚕豆种子萌发及幼苗生长,并随处理浓度增加,抑制效应加强,对叶片数和根系的抑制效应最为显著。肉桂酸低浓度处理时显著促进尖孢镰刀菌菌丝生长,而高浓度处理则抑制菌丝生长。肉桂酸在100和200 mg · L^-1处理时显著提高了蚕豆枯萎病的病情指数,显著增加了根际土壤中镰刀菌的数量,降低了微生物的活性（AWCD值）、多样性和丰富度指数。主成分分析表明,肉桂酸处理明显改变了蚕豆根际微生物群落结构,糖类、羧酸类和氨基酸类是区分肉桂酸处理导致土壤微生物群落变化的敏感碳源。肉桂酸处理降低了根际微生物多样性,促进病原菌的增殖,加剧了蚕豆枯萎病的发生,证实肉桂酸是蚕豆连作障碍中的自毒物质。  相似文献