取食转Bt基因棉对斜纹夜蛾幼虫存活的影响   总被引：3，自引：0，他引：3  

郭建英  董亮  万方浩 《中国生物防治》2003,19(4):145-148

以常规棉泗棉3号为对照,测定了取食转Bt基因棉GKl2的不同部位(花、蕾、铃和不同空间部位叶片)10d对斜纹夜蛾初孵幼虫存活率的影响。结果表明：棉花品种和植株部位对斜纹夜蛾幼虫存活率均产生显著影响,但两者之间没有交互作用。取食Bt棉顶部新叶(倒1叶)、中上部展开叶片(倒3叶和倒6叶)和蕾10d的斜纹夜蛾幼虫,其存活率与对照没有显著差异。取食Bt棉下部成熟叶片(倒14叶)4d后,幼虫存活率方显著低于对照。取食Bt棉花朵的幼虫初期(≤4d)存活率低于对照,但随着对照死亡率的增加,6～10d内两者差异不再显著。取食Bt棉棉铃的幼虫存活率始终低于对照,但两者差异基本不显著。根据GKl2对棉铃虫和斜纹夜蛾杀虫效果的比较,初步探讨了斜纹夜蛾为害趋势加重的原因。  相似文献   

浅谈牧草转基因育种研究进展     

达娃东周  范月君 《青海草业》2006,15(3):24-26,40

简要概述了牧草遗传转化方向及牧草转基因所取得的主要贡献,介绍了牧草遗传转化三种方法,并就今后的发展前景和转基因育种生物安全问题做了初步探讨。  相似文献   

转Bt基因棉种植前后3个棉铃虫种群对4种杀虫剂的抗性变化     

郭庆龙  王开运  夏晓明  刘振龙  胡燕 《农药学学报》2006,8(3):255-259

分别于1990年、1992年、2001年和2005年从山东泰安、山东夏津和湖北天门采集3个棉铃虫H elicoverpa arm igera种群,采用点滴法测定了4种杀虫剂对其的毒力,以监测各种群的抗药性变化趋势。结果表明:19901992年3个种群棉铃虫对氰戊菊酯的抗性增长迅速(13.256.1倍);19922001年抗性增长速度有所减缓(3.111.0倍);20012005年抗性水平降低。3个种群棉铃虫对灭多威和辛硫磷的抗性变化趋势与氰戊菊酯的基本相似,但抗性水平较低;对硫丹则均一直处于敏感水平。引起抗药性变化的原因主要与各类药剂的使用频率和转B t基因抗虫棉的大面积推广有关。  相似文献   

转EGFP基因山羊克隆胚的发育     

郑月茂  刘凤军  安志兴  李向臣  赵晓娥  权富生  张涌 《畜牧兽医学报》2007,38(5):458-463

利用脂质体包裹含EGFP基因的质粒,并将之导入山羊乳腺上皮细胞,经G418筛选获得阳性细胞,以阳性细胞作为核供体,利用核移植技术构建转基因克隆胚。结果表明：用转基因山羊乳腺上皮细胞作为核供体,电融合法更适合构建转基因克隆胚。转基因山羊克隆胚体外培养最佳方案是用SOFaa培养液,在培养72 h后加入10%的正常山羊血清（Normal goat serum,NGS）。荧光显微镜下观察到转基因克隆胚中EGFP的表达,大部分克隆胚发育到8-16细胞以后的时期,绿色荧光蛋白才开始逐渐表达,随着发育时间的延长,绿色荧光蛋白的表达也逐渐增强。说明外源基因在胚胎早期发育阶段可以表达,EGFP可以作为报告基因来实现对外源基因整合及表达的监测。  相似文献   

精子载体法获得转人her2基因猪     

任红艳  张兴举  杨述林  李新云  单同领  靳二辉  唐中林  崔文涛  李奎 《中国畜牧兽医》2007,34(3):50-52

将带有外源基因的载体酶切纯化后与去精清的猪新鲜精液按比例混合，17℃静置处理使外源DNA进入精子头部，通过人工授精使受体母猪怀孕后产下20头仔猪，经PCR特异性片段扩增，特异片段序列测定和比对，共4头PCR结果为阳性。初步证明人her2基因已在猪染色体上整合，其整合率约为20％。本研究利用精子载体法成功获得了转基因阳性猪，为大动物的转基因研究提供了理论与实践的参考。  相似文献   

An antisense coat protein gene confers immunity to potato leafroll virus in a genetically engineered potato   总被引：2，自引：0，他引：2  

Andrzej Pałucha  Włodzimierz Zagórski  Mirosława Chrzanowska  Danuta Hulanicka 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(3):287-293

The Bzura commercial potato cultivar was transformed by sense or antisense constructs which included the coat protein gene of potato leafroll virus RNA. In the sense construct, the coat protein gene was preceded by a leader sequence shorter than that in the subgenomic RNA formed in infected cells. The antisense construct consisted of a sequence complementary to the first 2020 nucleotides of the subgenomic RNA. Selected transformants expressing viral RNA were resistant to virus challenge by viruliferous aphids. In one line, expression of the antisense RNA prevented virus infection even after grafting with scions from infected plants and therefore this transformant might be regarded as virus immune.  相似文献   

农业转基因生物对土壤生态系统功能影响的研究进展     

徐征 《勤云标准版测试》2004,(4)

土壤生态系统的功能是否正常直接关系到农业系统的稳定,影响土壤生态系统功能的因素已经不是局限在化学物理污染上了;农业转基因技术的应用,为农业生产带来新的增长点,为消除传统的化学物理污染的同时,也给土壤生态系统带来了更深刻,难以控制的影响因子:转基因生物污染。  相似文献   

Assessment of Rhizospheric Microorganisms of Transgenic Populus tomentosa with Cowpea Trypsin Inhibitor (CpTI) Gene     

Zhang Qian  Zhang Zhi-yi  Lin Shan-zhi  Lin Yuan-Zhen  Yang Le 《中国林学(英文版)》2005,7(3):28-34

To have a preliminary insight into biosafety of genetically transformed hybrid triploid poplars (Populus tomentosa × P. bolleana) × P. tomentosa with the cowpea trypsin inhibitor (CpTI) gene, two layers of rhizospheric soil (from 0 to 20 cm deep and from 20 to 40 cm deep, respectively) were collected for microorganism culture, counting assay and PCR analysis to assess the poten-tial impact of transgenic poplars on non-target microorganism population and transgene dispersal. When the same soil layer of suspension stock solution was diluted at both 1:1 000 and 1:10 000 rates, there were no significant differences in bacterium colony numbers between the inoculation plates of both transgenic and non-transgenic poplars. The uniform results were revealed for both soil layer suspension solutions of identical poplars at both dilution rates except for non-transgenic poplars at 1:10 000 dilution rates from the same type of soil. No significant variation in morphology of both Gram-positive and Gram-negative bacteria was observed under the microscope. The potential transgene dispersal from root exudates or fallen leaves to non-target microbes was repudiated by PCR analysis, in which no CpTI gene specific DNA band was amplified for 15 sites of transgenic rhizospheric soil samples. It can be concluded that transgenic poplar with the CpTI gene has no severe impact on rhizospheric microorganisms and is tentatively safe to surrounding soil micro-ecosystem.  相似文献   

Molecular Cloning of a Cellulose Synthase Gene PtoCesA1 from Populus tomentosa and Its Genetic Transformation in Tobacco     

Li Chun-xiu Zhang Shou-gong Wang Yang-dong Shi Sheng-qing Qi Li-wang 《中国林学(英文版)》2005,7(4):5-10

A 3 125 bp cellulose synthase gene, PtoCesA1, which has a 98% identity to PtrCesA1 from Populus tremuloides, was cloned from cDNA prepared from secondary xylem of P tomentosa. Four anti-expression vectors with different fragments of PtoCesAl, named as pBIPF, pBICC1, pBIPR and pBIBR, were constructed. Some traits of transformed tobacco of pBICC1, pBIPR and pBIBR differed from wild types, such as small leaves, ＂dwarf＂ phenotype and thinner xylem and fiber cell walls than wild plants consistent with a loss of cellulose. It indicated that the growth of transgenic tobacco was restrained by the expression of anti-PtoCesA1. Transgenic tobacco was obtained and the contents of cellulose and lignin were analyzed as well as the width and length of fiber cells, and xylem thickness for both transgenic and control plants. Transformed tobacco showed a different phenotype from control plants and it implied that PtoCesA1 was essential for the cellulose biosynthesis in poplar stems.  相似文献   

转基因741杨对舞毒蛾不同龄期幼虫的抗性研究   总被引：4，自引：0，他引：4  

高宝嘉  胡海燕  王进茂  梁海永  段文倩 《河北林果研究》2002,17(2):159-162

用7种不同系号的转抗741叶片为材料，对舞毒蛾幼虫进行逐龄分别饲养，系统测定各系号抗虫杨叶片对舞毒蛾不同龄期幼虫的死亡率，体重增长速率，发育速率的影响。结果表明：7种抗虫杨叶片对1龄幼虫均表现出较高的毒杀作用，其中处理3d的死亡率均达到35％，处理5d死亡率达80％。对2，3龄幼虫除具有一定的毒杀作用外，主要表现为对其生长发育的抑制作用，影响其正常的取食，降低体重增长速率和减慢发育速率。  相似文献