Characterisation of energy-dependent efflux of imazalil and fenarimol in isolates of Penicillium italicum with a low,medium and high degree of resistance to DMI-fungicides   总被引：2，自引：0，他引：2  

J. Guan  J. C. Kapteyn  A. Kerkenaar  M. A. De Waard 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(5):313-324

Differential accumulation of [¹⁴C]imazalil and [¹⁴C]fenarimol by germlings of wild-type and DMI-resistant isolates ofPenicillium italicum was studied at various pH values. At pH 7 and 8 the low-resistant isolate E_300–3 accumulated 22% and 35%, respectively, less imazalil than the wild-type isolate W₅. Imazalil accumulation at pH 5 and 6 was similar. Isolate E_300–3 also accumulated less fenarimol as compared with the wild-type isolate. This difference was much more obvious than for imazalil and was observed at all pH values tested. Differences in accumulation of both imazalil and fenarimol between low (E_300–3), medium (H₁₇) and high resistant (I₃₃) isolates were not observed. These results suggest that decreased accumulation of DMIs is responsible for a low level of resistance only and that additional mechanisms of resistance might operate in isolates with a medium and high degree of resistance. With all isolates fenarimol accumulation was energy-dependent. This was not obvious for imazalil.The wild-type and DMI-resistant isolates had a similar plasma membrane potential as determined with the probe [¹⁴C]tetraphenylphosphonium bromide ([¹⁴C]TPP⁺). Various test compounds, among which ATPase inhibitors, ionophoric antibiotics and calmodulin antagonists, affected the accumulation of [¹⁴C]TPP⁺, [¹⁴C]imazalil and [¹⁴C]fenarimol. No obvious correlation between the effects of the test compounds on accumulation levels of the fungicides and [¹⁴C]TPP⁺ could be observed. These results indicate that the plasma membrane potential does not mediate the efflux of DMI fungicides byP. italicum.  相似文献   

某类高阶非线性偏微分方程组弱解的正则性     

简怀玉 《湖南农业大学学报(自然科学版)》1990,17(3)

本文研究一类2k阶非线性偏微分方程组之解的正则性,没有假定通常的椭圆性条件而只假定所谓"无穷远处"的椭圆性条件,证明了解的k-1阶导数为李普希兹连续的.  相似文献   

The effect of some Frescon analogs on the aquatic snail,Lymnaea stagnalis: II. Correlation of neurotoxicity to Molluscicidal effectiveness     

B.L. Brezden  D.R. Gardner 《Pesticide biochemistry and physiology》1980,13(2):178-188

Although the molluscicide Frescon is a strong neurotoxin to the Lymnaea stagnalis central nervous system in vitro, it is probable that the exposure of the whole animal to this molluscicide fails to result in central nervous system abnormalities: Frescon does not appear to reach the brain in sufficient quantity to disrupt its normal activity. However, only those Frescon analogs found to be neurotoxic were molluscicidal, suggesting some related mode, if not site, of action. Frescon and its analogs may act by affecting excitable tissues other than the nervous system (e.g., the snail musculature) by altering certain functional and/or structural membrane properties.  相似文献   

用吹扫捕集法测定十字花科蔬菜中挥发性物质   总被引：1，自引：0，他引：1  

何洪巨  唐晓伟  宋曙辉  王文琪  刘玲 《中国蔬菜》2005,(Z1):39-42

采用吹扫捕集技术(Purge and Trap technique)对不同十字花科蔬菜中的挥发性成分进行了富集,以气质联用仪(GC／MS)进行鉴定。结果在白菜、羽衣甘蓝、芥蓝、青花菜、芥菜和大白菜中共鉴定出86种挥发性物质,化合物的种类包括醇类、醛类、酮类、酯类、硫醚类等,同时还发现了硫苷降解产物异硫氰酸盐和腈类等化合物。  相似文献   

中国风水侵蚀交错区分布特征分析   总被引：6，自引：1，他引：6  

邹亚荣  张增祥  王长有  赵晓丽  刘斌 《干旱区研究》2003,20(1):67-71

风水侵蚀交错带的研究 ,对景观、土地覆盖 /土地利用、全球变化等研究具有重要意义。论文以GIS技术为支撑 ,定量计算出我国风水侵蚀带。在分布特征上 ,主要分布我国北方地区 ,其中以西部沙漠 ,北部山脉沿线为重 ,这种分布与气候、地貌分界有明显联系。 49.6 %风水侵蚀复合带的降水量小于 2 0 0mm。风水侵蚀复合带的土地利用主要以草地为主 ,占 41.8% ,其次为耕地。两种类型的分布 ,草地以与其它土地利用类型交错分布为主 ;而耕地是有重心的分散分布。风水侵蚀复合带的总体侵蚀要强于全国水平。  相似文献   

Expression and role of CD134 and NF-κB in renal tissue of lupus nephritis     

ZHOU Yan-bin  YE Ren-gao  XIE Can-mao  XU Han-shi  GUAN Wei-ming  YANG Xiao  YANG Nian-sheng 《园艺学报》2003,19(9):1221-1223

AIM:To investigate the role of CD134 (OX40) and NF-κB in the pathogenesis of lupus nephritis (LN).METHODS:Renal in situ CD134 and NF-κB expression were examined in 40 biopsy specimens from LN patients by immunohistochemistry and microwave-based immunohistochemistry, respectively. The relationship between expression of CD134 and NF-κB was analyzed.RESULTS:The expression of glomerular and tubular CD134 and NF-κB in LN were higher than that in normal control, especially in class Ⅳ LN, where there was intense staining of endothelial cell, distal tubules, and interstitial mononuclear cell. The CD134 expression of glomerular and tubular was closely related to NF-κB expression, respectively (r=0.5542, P＜0.05;r=0.6279, P＜0.05). CONCLUSIONS:The abnormal expression of costimulatory molecule CD134 was well evidenced in LN. Strong expression of renal in situ NF-κB was likely mediated by CD134 signal pathway, which may play an important role in the pathogenesis of LN.  相似文献   

Effect of tea-polyphenols on the activation of NF-κB and the expression of TGF-β₁ mRNA in THP-1 cells incubated with VLDL,LDL or ox-LDL     

ZHANG Xiao-gang  CHEN Yun-zhen  LEI Han  WANG Zhou-bi 《园艺学报》2003,19(10):1398-1402

AIM:To investigate the effect of tea-polyphenols (TP) on the activation of NF-κB and the expression of TGF-β₁ mRNA in THP-1 cells (a human acute monocytic leukemia cell line). METHODS:THP-1 cells were incubated with the different concentrations of TP, VLDL, LDL or ox-LDL. In the THP-1 cellls, the nuclear malposition rate of NF-κB was detected with immunohistochemistry technique, the positive index of the TGF-β₁ mRNA expression was detected by hybridization in situ, and accumulation of total cholesterol (TC) in cells incubated with 0.4-40 μg/L TP was determined with oxidase assay. RESULTS:The nuclear malposition rate of NF-κB, the positive index of the TGF-β₁ mRNA expression and TC in THP-1 cells incubated with 0.4-40 μg/L of TP were lower than those with 0 μg/L of TP in TP-V group, TP-L group and TP-O (P＜0.05). The differences of these markers in THP-1 cells incubated with more than 40 μg/L TP in TP-V group, TP-L group and TP-O were not statistically significant, compared with TP-C group (P＞0.05). CONCLUSION:TP inhibited the activation of NF-κB, the expression of TGF-β₁ mRNA and the foam cell formation in the mono-macrophage.  相似文献   

腐败组织中毒鼠磷的GC／NPD、GC／ECD检验     

赵敬真  于忠山  王天宇 《农药科学与管理》2002,23(3):16-17

本文初步建立了一套混合溶剂提取、中性层析氧化铝小柱净化及GC／ECD和GC／NPD分析的腐败生物样材中毒鼠磷的检验方法。GC／ECD的检出限为1ng;GC/NPD的检出限为20ng。  相似文献   

梨自交不亲和强度不同品种花柱S糖蛋白含量的差异   总被引：12，自引：2，他引：12  

张绍铃  杨记磙  李秀根  平塚伸 《园艺学报》2002,29(2):168-167

 提取梨( Pyrus) 自交不亲和强度不同品种花柱可溶性蛋白质, 应用等电聚焦电泳法在凝胶板上分离出S 糖蛋白, 用图像解析法进行定量。结果表明, 花柱总S 糖蛋白含量因品种而异, 自交不亲和强、弱及自交亲和的品种每1 􀀁g 花柱可溶性蛋白中含总S 糖蛋白分别为􀀂1. 3 ng 、0. 7 ~ 0. 9 ng、 0. 7 ng 。不同的S 基因所表达的S 糖蛋白量差异显著, 即使是同一S 基因所对应的S 糖蛋白量也因品种而异。  相似文献   

苹果的3种优良砧木     

于福顺  焦功强  江志训 《落叶果树》2002,34(2):16-16

介绍了“B9”、“CG80”、“75-7-1”三种苹果矮化钻木，并以生产上应用较多的M26作为对照，研究了其矮化程度、早果性、嫁接亲和力、树体产量等性状。  相似文献