酵母基因表达调控关系的构建及其统计特性分析   总被引：1，自引：0，他引：1  

吉冬梅 《安徽农业科学》2009,37(10):4360-4362

[目的]从复杂网络的角度研究酵母细胞周期调控网络的统计特性。[方法]首先对酵母的细胞周期基因表达数据采用皮尔逊相关性度量,计算基因间的关联矩阵,即建立简单的基因调控网络;同时为了能够得到在宏观角度对大规模的基因调控有一个全貌的了解,进一步分析所建立调控网络的统计特性,通过计算所建立网络的统计特性：即计算网络的聚类系数、平均路径长度和网络的度分布。[结论]发现所建立的网络具有无标度和小世界特性,即所建立的酵母基因调控网络属于复杂网络,这为进一步深入研究基因调控网络的统计学和动力学特性打下了基础。  相似文献   

基于Matlab的AHP分析程序设计     

陈冠铭  贾虎  林亚琼 《农业网络信息》2012,(7):21-22,49

层次分析法是目前最为广泛应用的决策方法,但涉及大量的矩阵运算,影响了该方法的推广。Matlab软件精于矩阵运算,与C语言结构类似,移植性强,本研究基于Matlab设计出了便捷高效的AHP计算机程序。  相似文献   

基于GIS与马尔可夫模型的渭河盆地景观动态变化研究   总被引：8，自引：1，他引：8  

张海龙  蒋建军  解修平  吴宏安  张丽 《干旱区资源与环境》2005,19(Z1):119-124

基于1988年与2003年两期TM影像,综合遥感与GIS技术以及景观生态学理论,深入分析了渭河盆地景观变化的数量和空间特征以及由此所引起的一系列生态环境效应,主要表现在:耕地、园地与城镇建设用地面积显著增加,林地、水体和未利用地面积呈减少趋势,城镇建设用地的增加是以侵占大量农田为代价的,而园地与耕地的增加则主要是通过开荒的方式。景观形状趋于复杂,破碎度降低,异质性下降,景观类型有向单一化或非均衡化方向发展的趋势,表明人类的开发活动增强,这样必然导致景观稳定性下降,因而降低了景观抗干扰的能力。最后采用马尔可夫模型对景观的变化趋势进行了预测。  相似文献   

Identification of ocular matrix metalloproteinases present within the aqueous humor and iridocorneal drainage angle tissue of normal and glaucomatous canine eyes   总被引：1，自引：0，他引：1  

William L. Weinstein  Ursula M. Dietrich  John S. Sapienza§  K. Paige Carmichael†  Phillip A. Moore  Thomas M. Krunkosky‡ 《Veterinary ophthalmology》2007,10(S1):108-116

  相似文献   

Estradiol stimulated proliferation and differentiation of prostatic stromal cells through regulation of BPH-1 paracrine     

WU Quan  XIAO Xiang-qian  LIU Shu-ye  LIU Yu  SHI Jian-dang  WANG Ke-ming  ZHANG Ju 《园艺学报》2007,23(7):1382-1387

AIM: To characterize the effect of estradiol on proliferation, differentiation and extracellular matrix (ECM) accumulation in stromal cells through regulation of BPH-1 paracrine. METHODS: BPH-1 cells were stimulated with different concentrations of estradiol. Conditioned media (CM) were harvested and their effects on stromal cell cultures were tested. Cell proliferation was determined by MTT assay. mRNA of smoothelin, fibronectin, collagen Ⅳ and transforming growth factor β₁(TGF-β₁) were analyzed by real-time RT-PCR. Western blotting was used to determine smooth muscle myosin heavy chain (SMMHC). ELISA and radioimmunoassay were respectively used to measure fibronectin, TGF-β₁ and collagen Ⅳ protein expressions.RESULTS: Estrodiol stimulated the expression and secretion of TGF-β₁ in BPH-1 cells. The proliferation of stromal cells increased when they were cultured with CM harvested from estrogen treated BPH-1 cells. The mRNA levels of collagen Ⅳ and smoothelin increased in stromal cells treated with CM from BPH-1 cells. The results of radioimmunoassay also showed that the collagen Ⅳ protein level up-regulated in the supernatants and cell extracts of CM-treated stromal cells. A neutralizing antibody to TGF-β₁ inhibited the stimulation of collagen Ⅳ and SMMHC by BPH-1 CM. The expression of fibronectin was only marginally changed in stromal cells cultured in the presence of BPH-1 CM. CONCLUSION: The BPH-1 cells increase ECM accumulation and differentiation of stromal cells through TGF-β₁. Estradiol stimulate differentiation of stromal cells by induction of TGF-β₁ expression. Estradiol stimulate proliferation by influencing the factors secreted from prostatic epithelial cells.  相似文献   

The spore coat of the bean anthracnose fungus Colletotrichum lindemuthianum is required for adhesion, appressorium development and pathogenicity     

Sarah L. Rawlings  Richard J. O&#x;Connell  Jonathan R. Green 《Physiological and Molecular Plant Pathology》2007,70(4-6):110-119

The spores (conidia) of the bean anthracnose fungal pathogen, Colletotrichum lindemuthianum, adhere to the aerial parts of plants to initiate the infection process. In previous studies we have shown that the Colletotrichum spores are surrounded by a fibrillar spore coat, comprising several major glycoproteins. Previous evidence showed that a monoclonal antibody (UB20) that recognised these glycoproteins was able to inhibit adhesion of spores to a hydrophobic surface. In this paper we have further studied the role of the spore coat in adhesion, germination and fungal development by studying the effects of UB20 and protease treatment of spores. The latter treatment has previously been shown to remove the spore coat. Spores germinate on glass, polystyrene and water agar, however, appressoria only develop on glass or polystyrene, showing a requirement for a hard surface. Removal of the spore coat with protease inhibits adhesion at 30 min, before the secretion of ECM glycoproteins. Protease treatment also inhibits the development of appressoria and reduces pathogenicity on leaves. Incubation of spores with the MAb UB20 inhibits adhesion at 30 min, but does not affect appressorium formation or pathogenicity. The results suggest that an intact spore coat has two functions; it is required for adhesion to a hydrophobic surface and for the detection of a hard surface necessary for appressorium formation. We suggest that contact with a hard surface, rather than adhesion, is the key event leading to appressorium formation.  相似文献   

Influence of Sucrose, Mucin and Xanthan Gum on Spore Germination of Ten Different Fungi     

G.S. Mahuku  P.H. Goodwin 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(8):849-852

Germination of conidia, uredospores and basidiospores of ten diverse fungi were compared in sterile distilled water, 0.5% sucrose, 0.5% mucin and 0.5% xanthan gum. All three compounds generally increased spore germination on cellophane membranes compared to water, but mucin was the most effective. Mucin stimulated significantly more germination than sucrose for 7 of the 10 fungi, and only Aspergillus niger and Colletotrichum gloeosporioides showed greater spore germination in sucrose than in mucin. Sucrose had no effect on uredospore or basidiospore germination, but mucin stimulated germination of these spores. Mucin was more effective than xanthan gum in increasing spore germination for 9 of the 10 fungi. Animal mucin is chemically similar to the natural spore matrix of some fungi and may provide some of the benefits of the spore matrix without the drawback of possible germination inhibitors. Animal mucin may be a useful substitute for the spore matrix in inoculations of a variety of fungi.  相似文献   

基于Lefkovitch矩阵模型的山酢浆草种群动态分析     

尤海梅  小池文人 《浙江林学院学报》2011,28(1):66-71

依据林下多年生草本植物山酢浆草Oxalis griffithii的形态学特征,将其生活史划分为4个阶段,并根据3种生境下山酢浆草种群的野外调查资料建立了不同种群的Lefkovitch结构矩阵模型,在此基础上预测了山酢浆草种群的动态及其阶段结构的变化;同时,运用敏感度、贡献率指标分析了影响种群动态的关键生活史阶段。结果表明：不同生境下山酢浆草种群的动态发展趋势不尽相同,常绿阔叶林下的种群（MD）呈稳定发展状态,其他2种生境下种群表现出衰退的发展趋势;种群的动态变化过程受个体的生长、残存、萎缩和繁殖等的综合影响;不同生活史阶段对种群动态的影响差异显著,种群动态过程及其发展趋势主要受种群内较大阶段个体的影响。图2表5参19  相似文献   

最佳线性无偏预测估计种畜育种值（BLUP）可视化计算机程序     

谢振宇  ;谢镇玲  ;蒋清洋 《计算机与农业》2008,(7):29-31

应用VB6可视化编程软件，设计较先进的最佳线性无偏预测估计种畜育种值（BLUP法）程序，增强了软件的功能，使其更具易用性、广泛性。  相似文献   

反应网络的物料平衡一致性校验     

范伟云  夏璐 《广西农业生物科学》1997,(1)

通过对反应网络的物料平衡分析，提出了反应网络的物料平衡一致性校验方程，并用于反应网络的数据校正和推测  相似文献