对应用氚水法测定林木蒸腾量的评价   总被引：7，自引：0，他引：7  

陈杰  齐亚东 《东北林业大学学报》1990,18(3):105-113

根据最近十多年应用氚水法测定树木蒸腾量有关资料,为了使此方法更为完善得予广泛应用,笔者于1985、1986两年通过对针叶树(红松)和阔叶树(柞树、杨树和桦树)的实验研究,进行了不同方法和准确度的比较和评价。例如氚水的引入方式和引入量,取样方法和取样部位等。考虑到氚水在树木体内的分布特点,应使氚水在木质部内均匀混合,取样部位也因随不同树种,不同环境条件而异。研究结果证明,氚水方法同其它方法比较确是一种测定林木蒸腾作用的切实可行的简便方法。  相似文献   

生长抑素与乙肝表面抗原融合基因重组痘苗病毒的构建及其表达   总被引：18，自引：0，他引：18  

徐文忠  李光地 《南京农业大学学报》1992,15(3):74-80

  相似文献   

荒漠草原风蚀地表颗粒空间异质特征   总被引：1，自引：1，他引：1  

丁延龙  蒙仲举  高永  娜仁格日勒  黄昕  刘斌 《水土保持通报》2016,36(2):59-64

[目的]研究希拉穆仁荒漠草原地区风蚀地表颗粒的空间异质特征,对研判风蚀地表退化程度,进而合理保护与利用该区草地资源提供支持。[方法]利用数字图像处理技术,获取了希拉穆仁荒漠草原坡面表层土壤颗粒组成信息,结合经典统计学和地统计方法,研究易风蚀颗粒(0.42mm)、中等颗粒(0.42~0.84mm)、不易风蚀颗粒(0.84mm)的空间分布格局。[结果]荒漠草原地表颗粒含量组成表现为:不易风蚀颗粒中等颗粒易风蚀颗粒;颗粒变异系数变化范围为11.23%~38.17%,属中等程度变异。3种颗粒最优半方差函数模型分别为指数、球状、球状模型;Kriging插值结果显示坡顶土壤颗粒组成最粗,自坡顶向下表现出变细的趋势;南坡与西南坡土壤粗化最严重,粗化程度由重到轻依次为:南(S)西南(SW)西北(NW)西(W)东南(SE)北(N)东北(NE)东(E)。[结论]研究区土壤颗粒具有强烈的空间自相关性,土壤颗粒组成空间变异性主要由结构性因素引起,人为干扰也产生了一定影响。  相似文献   

结合条件对羊抗兔抗体连接到细菌磁小体的影响     

陈继峰  李颖  姜伟  王珍芳  孙建波  李健  李季伦  李绍华 《农业生物技术学报》2006,14(3):423-428

用羊抗兔抗体进行抗体连接到细菌磁小体(BMPs)实验，以探讨结合条件对抗体连接效率(以每毫克BMPs上连接抗体的微克数表示，单位为μg/mg)的影响。实验表明，冷冻干燥后的BMPs的抗体连接效率降低36%~55%；在液氮中(-196 ℃)预处理的抗体连接效率显著高于在-70 ℃和-20 ℃预处理的。室温(15 ℃)下反应18 h的抗体连接效率最高，为64.06 μg/mg，明显高于其它温度和连接时间的。反应体系中，BMPs用量由5 mg降到0.1 mg，抗体用量由10 μg增加到300 μg时，抗体连接效率逐渐上升，当BMPs用量为0.1 mg，抗体用量为300 μg时，抗体连接效率达762.37 μg/mg。PBS、HEPES、Tris-HCl、MOPS、VBS以及磷酸盐等溶液可用于抗体连接到BMPs体系中，在通常使用的缓冲液pH和浓度下，几种溶液的抗体连接效率变化范围为46.28±0.58 ~ 90.83±1.64 μg/mg；并且不同的溶液有相应的使抗体连接效率达到最高时的pH和溶液浓度，当HEPES的pH为3.5，浓度为20 mmol / L；Na3PO4的pH为5.6，浓度为5 mmol / L时，抗体连接效率分别为108.01和76.78 μg/mg。对连接到BMPs上的抗体进行SDS-PAGE定性检测结果表明，抗体连接到了BMPs的膜上。  相似文献   

PREPARATION AND APPLICATION OF ULTRAFINE PARTICLES     

Zhang Qichao Yang Jun LU Rongzhong 《保鲜与加工》1989,(2):88-94

In this paper, basic principles and methods about preparing ultrafine particles at home and abroad are systematically introduced, and the ma-in species of ultrafine particles and their future applications are briefly discus- sed.  相似文献   

鸡传染性法氏囊病病毒rVP2蛋白高效组装亚病毒颗粒的鉴定     

王彦伟  张素玲  吴芃  耿笑林  黄玉欣  李鹏昊  王孟月  李向东  逄文强  田克恭 《中国畜牧兽医》2020,47(6):1677-1684

为获得高纯度的鸡传染性法氏囊病病毒（infectious bursal disease virus,IBDV）VP2蛋白自组装的亚病毒颗粒（subviral particles,SVPs）,并对重组VP2蛋白（rVP2）SVPs的组装效率和均一性进行评价,本试验利用大肠杆菌表达IBDV rVP2,通过SDS-PAGE和Western blotting对蛋白表达情况和免疫反应性进行鉴定,通过阴离子交换层析和切向流超滤浓缩系统纯化rVP2蛋白,并通过透射电镜（TEM）、高效液相尺寸排阻色谱（HPSEC）、蔗糖密度梯度离心及粒径和表面电位（Zeta potential）等多种检测技术对单个SVP组装形态和整体组装情况进行分析检测,初步建立了IBDV rVP2 SVPs组装效率的系统性检测方法。结果显示,在大肠杆菌中实现IBDV rVP2的大部分可溶性表达,且能与鸡IBDV阳性血清有明显的免疫反应性,纯化后rVP2纯度提高66.9倍,回收率达到93.3%。TEM观察rVP2自组装成直径约25 nm的SVPs,视野内SVPs大小均一,均匀分散。HPSEC检测结果显示,rVP2 SVPs的分子质量约为2 482 ku,与20个VP2三聚体形成的T=1二十面体SVPs分子质量一致。蔗糖密度梯度离心检测结果显示,rVP2 SVPs体积分布均一,组装效率高。粒径和Zeta电位检测结果表明,rVP2 SVPs颗粒分散度较好、体系稳定,有利于rVP2 SVPs的长期保存。本试验通过多种检测技术对rVP2 SVPs的组装情况进行检测分析,实现了rVP2 SVPs的高效组装,为IBDV SVPs疫苗的质量控制提供了有力支撑。  相似文献   

1KGL-3型保水保肥联合整地机的研制     

张旭  尤晓东  章惠全  程晋 《农业科技与装备》2021,(1)

将秸秆压缩成颗粒后再进行还田作业是改良土壤、培肥地力的新途径。研制一种能将秸秆颗粒施入地表下实现还田效果的保水保肥联合整地机。介绍该机具的总体结构、工作原理及关键部件设计,并检测其作业质量。该机具可实现秸秆颗粒掩埋、起垄、镇压等复式联合作业。  相似文献   

Bacterial nutrition of great scallop larvae     

J. Moal  J. F. Samain  S. Corre  J. L. Nicolas  A. Glynn 《Aquaculture International》1996,4(3):215-223

Bacterial nutrition of great scallop, Pecten moximus, larvae was investigated using the radioactive tracer technique. The bacterial labelling was studied initially to obtain a high and stable specific radioactivity (¹⁴C) of bacterial cells. A higher bacterial specific ¹⁴C activity was obtained when the tracer (amino acid) was introduced in the culture medium at the beginning of the exponential growth phase. After a 12 h labelling period in a rich nutritive medium, the bacteria were depurated in seawater for 5 h (chase) to prevent further ¹⁴C excretion and then added to larval rearing vessels. The larval labelling was followed for 12 h and then larvae were placed in new vessels without radioactive bacteria. The depuration of larvae was followed for 3 days. Data obtained on ingestion and assimilation efficiency show that bivalve larvae are able to ingest and digest bacteria.  相似文献   

Observation of virus particles in the spleen, kidney, gills and erythrocytes of Atlantic salmon, Salmo salar L., during a disease outbreak with high mortality     

D A Graham  W Curran  H M Rowley  D I Cox  D Cockerill  S Campbell  & D Todd 《Journal of fish diseases》2002,25(4):227-234

An annual disease problem with high levels of mortality in Atlantic salmon, Salmo salar L., on a freshwater farm was investigated. In 2000, mortalities began early in October and peaked in December, being unevenly distributed between tanks. Histopathological changes included severe hyperplasia of gill epithelium, with fusion of secondary lamellae, and extensive necrosis of the haematopoietic centres in the kidney and spleen, the latter being consistent with a diagnosis of phagocytolytic syndrome (PCLS). Moribund fish were anaemic, with multiple, circular basophilic inclusion bodies, 1–2 μm in diameter and suggestive of erythrocytic inclusion body syndrome (EIBS), being observed on blood smears. Thin section electron microscopy (EM) revealed primarily membrane-bound aggregates of non-enveloped virus particles (73 ± 7 nm) with an electron dense core (35 ± 3 nm) in spleen, kidney and gill tissue and the erythrocytes therein. These particles had morphology and distribution consistent with those previously described separately for both EIBS and PCLS. Virus isolation attempts in a wide range of cell lines were unsuccessful. In the absence of suitable alternative infectious or environmental explanations, it is postulated that the aetiology was primarily viral, with possible contributory secondary environmental factors. It is further hypothesized that the agents of EIBS and PCLS may be the same or closely related viruses.  相似文献   

Numerical simulation of gas solid characteristics in double inlet square cyclone separator     

LIU Han zhou  LU Xiao feng  HUANG Yong jun 《保鲜与加工》2011,(6):81-86

This paper uses RSM turbulence model to conduct the numerical simulation on the gas flow field in double inlet square separator and calculate the velocity field and pressure distribution in the seperator. At the same time, it simulates the solid particle trajectories in the separators using Lagrangian model. The results show that the velocity field and pressure distribution in double inlet square separator have good symmetry, but the flow swirl intensity is insufficient and easy to decay; different sizes and different injection locations bring significant differences for the particle trajectories; injecting small particles can be easy to form flow short-circuit phenomenon inside the separator, injecting particles near the top of separator can be easy to form top ash ring phenomenon inside the separator; these phenomena have adverse factors to the separation efficiency. When the separation cylinder corners of square separator are changed into cutting-shaped chamfers, the flow field in the separator can be improved, and the separation efficiency of separator can be effectively improved.  相似文献