茶叶中甲基化EGCG的分离及功能研究进展     

禹双双  陈丝  罗奕凡  王坤波 《茶叶通讯》2015,(3):3-7

综述了茶叶中甲基化EGCG的分离方法和功能研究进展。茶叶甲基化EGCG分离方法有制备液相色谱(p HPLC)、高速逆流色谱(HSCCC)、Sephadex LH-20凝胶柱层析、Toyopearl HW-40S中压柱层析等,主要具有抗过敏、保护肝脏、调节肠道菌群、治疗哮喘、清除自由基等功效。  相似文献   

Measurement variability in soybean water status and soil‐nutrient extraction in a row spacing study in the U.S. southeastern coastal plain     

《Communications in Soil Science and Plant Analysis》2012,43(9):1111-1134

Abstract

Full‐season determinate soybean [Glycine max (L.) Merrill] was grown in the field in a humid climate for three seasons (1979–81). The objective was to examine variability in several methods of determining basic relationships between soil and plant water status in a range of canopy configurations and to examine treatment effects on soil‐nutrient extraction. In each year, two cultivars, “Davis” (group VI) and “Coker 338” (group VIII) were planted in four row spacings. In 1980 and 1981 the experiment was expanded and split for irrigation and row orientation (N—S or E‐W). Post‐harvest soil samples were collected and analyzed to determine if irrigation, row spacing, or cultivar influenced K, Ca, and Mg extraction patterns.

During the growing seasons, parallel leaf diffusive resistance (R_s) was poorly correlated with xylem pressure potential (ψ_x), canopy s x temperature (T_c), canopy minus air temperature (?T), leaf vapor pressure deficit (LVPD), and atmospheric vapor pressure deficit (VPD) in single factor correlations. Xylem pressure potential was highly correlated with T_c , ?T, VPD, and LVPD, but was poorly correlated with soil water potential. Both ψ_x and T_c were significantly affected by the imposition of shade from a 60% shading cloth within as little as 1 minute of shade imposition. The impact of cultivar on seasonal ψ_x was significant and was nearly half the magnitude of the observed difference caused by irrigation. Irrigation raised ψ_x by only 2.2 bars over the two—year observation period, in spite of large differences in soil water potential when irrigation was imposed. The impact of canopy configuration was not measureable in any water relations parameter except infrared‐determined T_c. Correlation of T and ψ_x was significantly more reliable when limited to a single variety, row spacing, and row orientation. Aspect of infrared temperature measurement also significantly affected observed T_c.

Analysis of post‐harvest soil samples indicated that narrow (50 cm) row spacing in 1980 and irrigation in 1981 significantly decreased post—harvest Mehlich No. I extractable K, but none of the cultural practices influenced extractable Ca or Mg at P(0.05). In 1980, ex‐tractable K within soybean rows was significantly greater than between rows. Similar trends were observed for Ca and Mg in 1980 and for all 3 nutrients in 1981, but those differences were not significant at P(0.05). Overall, these measurements quantify the difficulty in relating soil and plant water status and identifying nutrient extraction patterns in sandy soils within the humid U.S. Southeastern Coastal Plain.  相似文献   

Nitrogen Fertilizer Replacement Value of the Liquid Fraction of Separated Livestock Slurries Applied to Potatoes and Silage Maize     

《Communications in Soil Science and Plant Analysis》2012,43(1):73-85

Separation of livestock slurries followed by reverse osmosis yields mineral concentrates (MCs) in which almost all nitrogen (N) is ammonium (NH₄)-N. The ability of MCs to substitute calcium ammonium nitrate (CAN), a common conventional mineral N fertilizer, was tested in two trials on a silty loam soil (ware potatoes, 2009 and 2010) and four trials on sandy soils (starch potatoes, 2009 and 2010; silage maize in 2010 and 2011). The N fertilizer replacement value (NFRV) of spring-injected MCs ranged from 72 to 84%, slightly less than their share of ammonium-N (90–100%). The fate of N that was apparently unavailable to crops was not fully disclosed, but there were indications that ammonia loss may have played a role.  相似文献   

Use of clomipramine in treatment of obsessive-compulsive disorder, separation anxiety and noise phobia in dogs: a preliminary, clinical study     

Seksel K  Lindeman MJ 《Australian veterinary journal》2001,79(4):252-256

OBJECTIVE: To evaluate the efficacy and tolerance of a treatment protocol for obsessive-compulsive disorder, separation anxiety and noise phobia in dogs. DESIGN: A study was undertaken to assess clinical responses in 24 dogs diagnosed with one or more of three behavioural disorders stated above to a treatment regimen that included clomipramine and behaviour modification. PROCEDURE: A detailed behavioural and clinical history was obtained for each dog. Obsessive-compulsive disorder was diagnosed in nine cases: primary presenting complaints were tail-chasing, shadow-chasing, circling and chewing; one case was diagnosed with concurrent separation anxiety. Separation anxiety was diagnosed in 14 cases: presenting complaints included destruction, vocalisation and escaping in the absence of the owner; four cases also exhibited noise phobia. The study also included one dog diagnosed with noise phobia only and another with inappropriate fear responses. Clomipramine was administered orally twice daily. The starting dose was 1 to 2 mg/kg bodyweight. The dose was increased incrementally to a maximum of 4 mg/kg if needed. A behaviour modification program was designed and the owner instructed on its implementation. Dogs continued medication for at least 1 month after clinical signs disappeared or were acceptably reduced, then withdrawal of medication was attempted by decreasing drug dosage at weekly intervals while behaviour modification continued. RESULTS: The presenting clinical sign was largely improved or disappeared in 16 dogs, 5 demonstrated slight to moderate improvement and the behaviour was unchanged in 3. Clomipramine withdrawal was attempted in nine cases: this was successful in five. CONCLUSION: Clomipramine was effective and well-tolerated in controlling signs of obsessive-compulsive disorder and/or separation anxiety and/or noise phobia in 16 of 24 assessable cases, when used in combination with behaviour modification, and improvement in clinical signs was noted in 5 others.  相似文献   

胚胎干细胞的研究进展   总被引：1，自引：1，他引：1  

许杰  马恒东  郝泽东 《中国畜牧杂志》2006,42(13):43-44

胚胎干细胞来源于附植前胚胎的内细胞团或附植后胎儿的原始生殖细胞具有发育全能性的细胞。本文从它的研究概况、生物学特性、胚胎干细胞的分离培养与建系等方面做一概述。  相似文献   

感染鸡日龄对球虫单卵囊分离的影响     

李赟  古少鹏  王敏霞  王海珍 《中国畜牧兽医》2007,34(5):96-98

选用5、8、11日龄的雏鸡进行鸡球虫单卵囊分离试验,研究不同感染日龄对鸡球虫单卵囊分离的影响。结果表明:在其他条件一致的情况下,从感染成功率、潜伏期、高峰期的出现时间及持续时间和从每只感染雏鸡收集到的球虫卵囊数量等指标进行综合判定,发现感染11日龄的雏鸡单卵囊分离成功率最高。  相似文献   

Growth and development of Lilium longiflorum ‘Nellie White’ during bulb production under controlled environments : II. Effects of shifting day/night temperature regimes on scale bulblets     

S.H. Kim  C.E. Niedziela Jr.  P.V. Nelson  A.A. De Hertogh  W.H. Swallow  N.C. Mingis 《Scientia Horticulturae》2007

One-year old scale bulblets of Lilium longiflorum Thunb. ‘Nellie White’ (Easter lily) were grown for 107 days during growth period 1 (GP-1) in six growth chambers under constant day/night temperature regimes of 30/26, 26/22, 22/18, 18/14, 14/10 and 10/6 °C. Subsequently, half of the plants in each temperature regime were transferred to 18/14 °C and the other half continued at the six constant temperature regimes. Both groups of plants were grown for an additional 89 days in growth period 2 (GP-2). Continuous temperatures of 26/22, 26/22–22/18 and 26/22–18/14 °C produced the greatest increase in basal bulb fresh weight (the main planted bulb), basal bulb circumference and stem bulb fresh weight, respectively. However, shifting these optimal temperatures to 18/14 °C during GP-2 resulted in a lower increase in basal bulb fresh weight and circumference. The optimum range for stem bulb production was expanded to 30/26–14/10 °C by shifting to 18/14 °C. The greatest increase for basal root growth occurred at 14/10–10/6 °C and for stem root growth at 14/10 °C. The temperature shift did not affect either root type. Maximum increase for stem length was at 26/22 and 22/18 °C and for stem plus leaf weight at 14/10 °C under constant temperature regimes. Transferring the plants from 10/6 to 18/14 °C resulted in the greatest increase in stem length and from 10/6 and 14/10 to 18/14 °C in the greatest increase in stem plus leaf weight. The greatest increase in the number of leaves occurred at 26/22 and 10/6 °C, but this growth parameter was unaffected by shifting to 18/14 °C, indicating that leaf number was determined in GP-1. Bulbils developed only when bulbs at high GP-1 temperature regimes (30/26 and 26/22 °C) were transferred to 18/14 °C during GP-2. Lower temperatures tended to favor an increase in flower bud production under continuous temperature regimes, while shifting to 18/14 °C increased flower bud production after initially high and low temperatures. Meristem abortion was greatest at 30/26 °C followed by 26/22 °C, but was not affected by temperature shifts in GP- 2. Thus, it is concluded that the abortion was induced or initiated during GP-1.  相似文献   

政企分开时政府、企业、银行三方利益均衡机制的构建分析     

李树丞  张佑林  李林 《湖南农业大学学报(自然科学版)》2000,(3)

提出了利益均衡机制的构造框架:研究目标、政企分开的基础研究,政企分开时银行、企业、政府三方决策过程研究,政企分开后的效果评价、实证研究、关键问题,提出了相应的构建思路.  相似文献   

淡水环境中可溶有机质研究进展     

贾国元  曾提  贾国东 《绿色科技》2013,(3):151-154

论述了淡水环境中可溶有机质(DOM)在分离与分析方法、来源与特征,以及环境意义等方面的研究进展。指出了在分离方法上,从XAD树脂分离到反渗透膜分离已经发展出各种分离技术;在分析方法上,现代波谱分析技术得到了广泛应用。DOM是由在来源、年龄、存在状态、后生改造等方面存在巨大差异的不同有机质混合构成的,因而呈现出十分复杂的非均质结构。DOM通过其自身的生物可利用性、改变有机污染物和重金属的生物可利用性及其耗氧能力影响环境质量,而且其自身的一些地球化学特征也能用来反映环境的变化特点。最后简述了湿地淡水环境中DOM的研究趋势和展望。  相似文献   

生物腔室构造与生物进化关系的研究——从生物腔室特征与其生命功能相适应的现象论证达尔文的生物进化论的科学观点     

曲树坤 《安徽农业科学》2013,(23):9621-9623

从生物的腔室的主要特征以及腔室的定义和基本特征着手,对腔室结构中的封闭性、牢固性、保护性和腔室之间的统一性进行分析,论证了腔室与其生命功能相适应是生物进化的结果这一观点,补证了达尔文生物进化论的观点,为探索物种起源和生命中的未知世界开辟了新途径.  相似文献