In vitro studies of Norwegian Red bovine semen immobilized and cryopreserved in alginate solid gel network          下载免费PDF全文

AH Alm‐Kristiansen  ER Gaustad  G Bai  FB Standerholen  G Klinkenberg  E Kommisrud  KE Waterhouse 《Reproduction in domestic animals》2018,53(2):365-370

Development of new semen cryopreservation techniques improving sperm survival and ensuring availability of viable spermatozoa for a prolonged time‐period after AI is promising tools to reduce sensitivity of timing of AI and enhance overall fertility. The SpermVital^® technology utilizes immobilization of bull spermatozoa in a solid network of alginate gel prior to freezing, which will provide a gradual release of spermatozoa after AI. The objective of this study was to compare post‐thaw sperm quality and in vitro sperm survival over time of Norwegian Red bull semen processed by the SpermVital^® (SV) technology, the first commercialized production line of SpermVital^® (C) and by conventional procedure applying Biladyl^® extender (B). Post‐thaw sperm motility was not significantly different between SV, C and B semen (p > .05). However, sperm viability and acrosome intactness were higher for SV than C and B semen (p < .05). Small differences in DNA quality were observed (p < .05). Sperm viability after storage in uterus ex vivo was higher for SV than for C semen (p < .05). Furthermore, sperm survival in vitro over time at physiological temperature was significantly higher for SV semen than C semen as well as B semen during the incubation period of 48 hr (p < .05). In conclusion, the SpermVital^® technology is improved and is more efficient in conserving post‐thaw sperm quality and results in higher sperm viability over time in vitro for SV than for C and B semen.  相似文献   

Heavy Metal Immobilization in Contaminated Soils using Phosphogypsum and Rice Straw Compost     

Esawy Mahmoud  Nasser Abd El‐Kader 《Land Degradation \u0026amp; Development》2015,26(8):819-824

The application of soil amendments to immobilize heavy metals is a promising technology to meet the requirements for environmentally sound and cost‐effective remediation. The present study was carried out to evaluate the result of phosphogypsum (PG) used alone and in combination with compost (CP) at a mix ratio of 1:1 wet weight ratio (PG + CP) at 10 and 20 g dry weight kg⁻¹ dry soil, on heavy metal immobilization in contaminated soil and on canola growth. The results revealed that the Pb, Cd and Zn uptake of canola plants was reduced by the application of PG alone and when it was mixed with CP as compared with untreated soil. At an application rate of 10 g dry weight kg⁻¹ dry soil of (PG + CP) the dry weight of canola plants increased by 66·8% was increased in comparison with its weight in the untreated soil. The addition of PG alone resulted in more pronounced immobilization of heavy metal as compared with PG mixed with CP. Plant growth was improved with CP addition, but heavy metals immobilization was the greatest in PG alone treatments. Results suggest that PG may be useful for the immobilization of heavy metals in contaminated soils. Copyright © 2014 John Wiley & Sons, Ltd.  相似文献   

Immobilization of wild giant panda (Ailuropoda melanoleuca) with dexmedetomidine–tiletamine–zolazepam          下载免费PDF全文

Yipeng Jin  Yanchao Qiao  Xiaobin Liu  Tianchun Pu  Hongqian Xu  Degui Lin 《Veterinary anaesthesia and analgesia》2016,43(3):333-337

  相似文献   

Nalbuphine and butorphanol reverse opioid‐induced respiratory depression but increase arousal in etorphine‐immobilized goats (Capra hircus)          下载免费PDF全文

Anna J Haw  Leith CR Meyer  Andrea Fuller 《Veterinary anaesthesia and analgesia》2016,43(5):539-548

  相似文献   

小鼠单精注射研究进展     

赵丹凤  赵立虎  刘丽均  张守纯  徐平 《动物医学进展》2006,27(9):14-17

自从Piezo操作系统应用于显微操作代替了传统的机械注入方法以来,小鼠胞质内单精注射的成功率就大大提高了。单精注射(ICSI)的基本原理并不复杂,但是许多研究表明小鼠精子的质膜、顶体、制动时间均会对ICSI的结果造成影响。文章概述了小鼠单精注射的基本原理及小鼠精子质膜的完整性,顶体反应,制动时间与ICSI成功率之间的关系,以期在此基础上提出今后的研究方向,对于分析受精过程的发生,改进单精注射技术及进一步提高受精率,都具有重要的理论意义及实践价值。  相似文献   

Immobilization of captive plains zebras (Equus quagga) with a combination of etorphine hydrochloride,acepromazine, and xylazine hydrochloride     

Tithipong PLANGSANGMAS  Hathaipat RATTANATHANYA  Wanlaya TIPKANTHA  Saowaphang SANANNU  Umaporn MAIKAEW  Jessada THAEONOEN  Bencharong SANGKHARAK  Pornpiroon CHINSON  Watcharin HIN-ON  Nathavut KANATIYANONT  Boripat SIRIAROONRAT  Nantarika CHANSUE  Parntep RATANAKORN 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):181

The plains zebra (Equus quagga) is a zebra species commonly kept in zoos around the world. However, they are not tame like their domestic relatives and are difficult to immobilize. We immobilized 30 captive plains zebra with a combination of etorphine hydrochloride (2–4 mg), acepromazine (8 mg), and xylazine hydrochloride (30 or 50 mg) to perform physical examination and blood sample collection for disease diagnostics. Physiological parameters including heart rate, respiratory rate, body temperature, and hemoglobin oxygen saturation were recorded. All zebras exhibited satisfactory anesthesia and fully recovered without re-narcotization. The results suggest that etorphine hydrochloride-acepromazine-xylazine hydrochloride combination for plains zebra immobilization is a safe and sufficient regimen for short procedures such as wellness examinations and sample collection.  相似文献   

土壤重金属钝化效果评估——基于大田试验的研究   总被引：4，自引：4，他引：0  

何赢  杜平  石静  秦晓鹏  徐刚  吴明红 《农业环境科学学报》2020,39(8):1734-1740

采用野外大田试验,研究了钙镁磷肥(Calcium-magnesia phosphate fertilizer)、麦饭石(Medical stone)、氧化铁吸附剂(Ferric oxide adsorbent)、氧化铝复合材料(Alumina composite)、植物型有机肥(Plant-based organic fertilizer)、牛粪(Cow dung)、骨炭(Bone char)对某地重金属污染农田的钝化修复效果,并利用动态加权评估方法评估了这几种钝化材料的综合适用性。试验结果表明:几种材料对土壤pH影响均较小,钙镁磷肥、麦饭石微量提高了土壤pH,与对照组相比分别增加0.68和0.74个单位;施加植物型有机肥、牛粪和骨炭提高了土壤有机质含量,与对照组相比分别增加了3.19、2.64 g·kg~(-1)和1.17 g·kg~(-1)(10.04%、8.31%和3.68%)。几种材料在0.6%的投加量下,除植物型有机肥外均有较好钝化效果,土壤有效态镉降低18%以上,且显著减少糙米中镉蓄积量(0.062～0.087mg·kg~(-1)),低于国家食品安全标准限值。为综合评估几种材料的效果及适用性,以土壤重金属有效态含量、糙米镉含量、修复成本和产量为指标构建动态加权函数综合评估模型,评估结果表明,钙镁磷肥的综合修复效果最好,其次为骨炭和植物型有机肥。  相似文献   

巯基化坡缕石对碱性土壤镉污染的快速钝化修复效应   总被引：1，自引：1，他引：0  

何丽质  徐应明  宋常志  吴义茜  黄青青  梁学峰 《农业环境科学学报》2021,40(2):319-328

为了探究巯基化坡缕石作为钝化剂在镉污染碱性土壤上的适用性及应用潜力,选取典型镉污染碱性土壤,以青菜和生菜为模式作物开展盆栽试验和土壤培养试验,研究巯基化坡缕石对碱性土壤镉污染的快速钝化修复效应。结果表明：巯基化坡缕石在应用剂量为0.1%~0.3%（m/m）时可以使生育期内青菜地上部镉含量降低81.44%~93.44%,根系镉含量降低68.47%~85.63%,生菜地上部镉含量降低65.11%~92.18%,根系镉含量降低57.77%~91.92%。收获时可食部位镉含量满足国家食品卫生标准限量值要求,在极低的应用剂量下显著的钝化效应是其优势。巯基化坡缕石可使土壤DTPA浸提有效态镉含量降低68.57%~84.10%,钝化过程速度快,5 d左右达到平衡,可以用二级动力学拟合。连续浸提形态分析显示,巯基化坡缕石能够快速减少土壤中可交换态和碳酸盐结合态镉含量,并显著提高易还原金属氧化物结合态镉含量,使镉由活泼态向不活泼态转化,从而减少作物吸收累积。巯基化坡缕石同时抑制了青菜和生菜对镉的转运系数和生物累积系数,进一步降低了可食部位镉含量。研究表明巯基化坡缕石是一种在镉污染碱性土壤上具有快速钝化作用和良好应用潜力的高效钝化剂。  相似文献   

小球藻固定化培养的初步研究   总被引：11，自引：0，他引：11  

杨海波  于媛  张欣华  李英敏 《水产科学》2001,20(5):4-7

用海藻酸钙作固定化载体，初步探讨了海藻酸钠浓度、CaCl2浓度、胶球密度、胶球直径等固定化条件对小球藻（Chlorella vulgaris)生长的影响。确定了优化的固定化条件为：4＃针头、2％的海藻酸钠溶液和0．15mol/L的CaCl2溶液制备固定化小球藻，在含50mL培养液的150mL三角烧瓶内放置250个小球藻胶球时，其生长速度较高，生长周期较长。与游离的小球藻相比，固定化小球藻生长速率慢，但生长周期长。实验结果为微藻固定化技术的应用和发展提供了必要的实验数据和理论基础。  相似文献   

一株海洋石油降解菌的特性分析及固定化研究     

关晓燕  吴垠  毛东东  姜冰  董颖  王召会  胡超魁 《河北渔业》2016,(5):1-5

从辽东湾沿岸受石油污染的沉积物中筛选得到一株石油降解菌株BHB-16,对该菌进行了形态以及16SrDNA系统发育分析,初步确定该菌株为Lutibacterium菌属。应用沸石和珊瑚石作为载体进行该菌株的固定化研究,确定当沸石作为载体时,固定化的最佳条件为:菌株接种量为0.6mL,培养时间28h,载体投加量为10mL;选用珊瑚石为载体时,固定化的最佳条件为:菌株接种量为0.6mL,培养时间29h,载体投加量为12mL;此外,用沸石和珊瑚石固定后的菌株,其对于柴油的降解率相对于游离菌分别提高了14.4%和29.6%,初步选用珊瑚石作为载体进行菌株的固定化使其具有更好的石油降解能力。  相似文献