盐胁迫对碱地风毛菊苗期脯氨酸代谢的影响   总被引：5，自引：3，他引：2  

夏方山  董秋丽  董宽虎 《草地学报》2010,18(5):689-693

通过对碱地风毛菊(Saussurea runcinata)苗期分别以NaCl和Na₂SO₄进行胁迫,设6个浓度和1个对照,测定其脯氨酸含量及δ-氨基转移酶、吡咯啉-5-羧酸合成酶和脯氨酸脱氢酶活性,以探讨其苗期耐盐性及脯氨酸代谢途径,并为盐碱地植被恢复与改良提供依据。结果表明:NaCl和Na₂SO₄胁迫下,碱地风毛菊的脯氨酸含量随NaCl和Na₂SO₄胁迫浓度增加而增高,在盐胁迫下,碱地风毛菊的脯氨酸合成途径以鸟氨酸途径为主;且在300mmol·L^-1的盐浓度胁迫下碱地风毛菊苗期的脯氨酸代谢最为旺盛,其对NaCl胁迫的耐性较高。  相似文献   

茯苓多糖对刺参体腔液中免疫因子活性的影响   总被引：5，自引：0，他引：5  

王淑娴  李天保  樊英  魏鉴腾  杨秀生  张伟  叶海斌  盖春蕾  许拉  刁菁  于晓清 《饲料研究》2010,(1)

采用水煎煮法提取茯苓多糖,苯酚-硫酸法测得茯苓多糖的含量为50.57%。利用分光光度技术,分析茯苓多糖对刺参体腔液中碱性磷酸酶(AKP)、溶菌酶(LSZ)、超氧化物歧化酶(SOD)活性及补体C3含量的影响。试验组向各组刺参体腔内分别注射质量浓度为0.6、1和1.4mg/mL的茯苓多糖溶液500μL。在注射免疫增强剂后第2、4和6天,检测AKP、LSZ和SOD活性及补体C3含量。结果显示,注射后6d内,试验组刺参体腔液中LSZ活性、SOD活性、补体C3含量及AKP活性分别在第4、4、4和6天达到最高,与对照组相比,差异显著,分别为对照组的5.5倍、1.2倍、2.6倍和1.5倍。注射0.3mg茯苓多糖的刺参AKP和LSZ活性高于另外2组,注射0.5mg茯苓多糖的刺参SOD活性和补体C3含量高于另外2组。研究结果表明:茯苓多糖能提高刺参的非特异性免疫水平,可作为刺参免疫增强剂使用,有成为刺参饲料添加剂的前景。  相似文献   

植物生理的研究在岩溶环境植被恢复中的应用     

曹宁  郭德俊  徐贤 《安徽农业科学》2010,38(36):20526-20527,20530

岩溶环境生态脆弱,影响了植(作)物对营养元素的吸收,妨碍了植(作)物的正常生长发育,做好岩溶地区的生态修复工作,最重要的就是该地区的植被恢复,这就需要选择岩溶环境适生植物,建立起具有生物多样性的稳定的岩溶森林生态系统,以保证系统能迅速地朝良性方向发展。而为了在众多植物种群中筛选出具有岩溶环境适生特性的品种,就需要首先对岩溶环境适生植物的生理特性诸如叶绿素荧光特性、碳酸酐酶活性、稳定碳同位素组成等作深入的研究,进而为建群种的选择提供一定的理论依据。  相似文献   

Combination of cell culture and quantitative PCR (cc–qPCR) to assess disinfectants efficacy on Cryptosporidium oocysts under standardized conditions     

M. Shahiduzzaman  V. DyachenkoJ. Keidel  R. SchmäschkeA. Daugschies 《Veterinary parasitology》2010

Oocysts of Cryptosporidium parvum are resistant to environmental conditions and many disinfectants. A combination of cell culture and quantitative real time PCR (cc–qPCR) is established for evaluation of anticoccidial disinfectants against C. parvum. C. parvum oocysts were treated with disinfectants, washed and oocysts were incubated with HCT-8 cell monolayers in the presence of excystation medium for 3 h. Subsequently, unbound parasites were removed by washing with growing medium and the infected monolayers were further maintained in fresh growing medium for 48 h. Genomic DNA was extracted from each sample and qPCR performed targeting a specific sequence of the 70 kDa heat shock protein gene in order to quantify development. Treatment of oocysts with cresolic disinfectants demonstrated dose dependent reduction of viability of oocysts. More than 98% inactivations were recorded with at least 2% concentration of cresolic disinfectants after 2 h of treatment. Bleach (sodium hypochlorite) at 6% solution induced 92.7% inactivation of C. parvum oocysts after 2 h. Thermally treated oocysts (56 and 70 °C for 20 min) demonstrated complete inactivation, whereas at 38 °C no inactivation was observed. Application of Neopredisan^® 135-1 and Aldecoc^® TGE (4% for 2 h) as recommended according to the current guidelines stipulated by DVG (German Veterinary Society) consistently inactivated more than 99.5% of oocysts. The suggested cc–qPCR method appeared to be suited for standardized testing of inactivation measures, particularly for evaluation of chemical disinfectants and thus cc–qPCR is proposed as an alternative to the established chicken infectivity model for Eimeria tenella for testing anticoccidial disinfectants. A minimum inactivation of 99.5% in cc–qPCR model is claimed as a suitable threshold for certification of chemical products for disinfection of coccidia oocysts.  相似文献   

Measurement of C‐peptide concentrations and responses to somatostatin,glucose infusion,and insulin resistance in horses     

F. TÓTH  N. FRANK  T. MARTIN‐JIMÉNEZ  S. B. ELLIOTT  R. J. GEOR  R. C. BOSTON 《Equine veterinary journal》2010,42(2):149-155

Reasons for performing study: Hyperinsulinaemia is detected in horses with insulin resistance (IR) and has previously been attributed to increased pancreatic insulin secretion. Connecting peptide (C‐peptide) can be measured to assess pancreatic function because it is secreted in equimolar amounts with insulin and does not undergo hepatic clearance. Hypothesis: A human double antibody radioimmunoassay (RIA) detects C‐peptide in equine serum and concentrations would reflect responses to different stimuli and conditions. Methods: A validation procedure was performed to assess the RIA. Six mature mares were selected and somatostatin administered i.v. as a primed continuous rate infusion, followed by 50 nmol human C‐peptide i.v. Insulin and C‐peptide concentrations were measured in horses (n = 6) undergoing an insulin‐modified frequently sampled i.v. glucose tolerance test, and in horses with insulin resistance (n = 10) or normal insulin sensitivity (n = 20). Results: A human RIA was validated for use with equine sera. Endogenous C‐peptide secretion was suppressed by somatostatin and median (range) clearance rate was 0.83 (0.15–1.61) ml/min/kg bwt. Mean ± s.d. C‐peptide‐to‐insulin ratio significantly (P = 0.004) decreased during the glucose tolerance test from 3.60 ± 1.95 prior to infusion to 1.03 ± 0.18 during the first 20 min following dextrose administration. Median C‐peptide and insulin concentrations were 1.5‐ and 9.5‐fold higher, respectively in horses with IR, compared with healthy horses. Conclusions: Endogenous C‐peptide secretion decreases in response to somatostatin and increases after dextrose infusion. Results suggest that relative insulin clearance decreases as pancreatic secretion increases in response to dextrose infusion. Hyperinsulinaemia in insulin resistant horses may be associated with both increased insulin secretion and decreased insulin clearance. Potential relevance: Both C‐peptide and insulin concentrations should be measured to assess pancreatic secretion and insulin clearance in horses.  相似文献   

mTOR对信号通路调控的研究进展   总被引：2，自引：0，他引：2  

綦松智  吴登俊  张中显 《中国畜牧杂志》2010,46(1)

哺乳动物雷帕霉素靶蛋白(mTOR)信号通路是最近新出现的细胞内重要信号途径,该途径在进化上高度保守,主要通过PI3K/Akt/mTOR信号通路磷酸化激活来调控细胞分裂、促进转录、信号翻译等,从而控制蛋白合成来调节细胞生长。mTOR作为一种重要的调节基因通过调节细胞周期、蛋白质合成、细胞能量代谢等多种途径发挥重要的生理功能,在细胞增殖、生长、分化过程中起着中心调控点的作用。  相似文献   

枳SPL9和SPL13全长cDNA克隆、亚细胞定位和表达分析   总被引：7，自引：1，他引：6  

宋长年  钱剑林  房经贵  王化坤  邱学林  章镇  张晓莹 《中国农业科学》2010,43(10):2105-2114

【目的】从枳[Poncirus trifoliata(L.)Raf.]中克隆SBP类[SQUAMOSA(SQUA)promoter-binding-like]转录因子基因SPL9和SPL13全长,构建SPL9和SPL13亚细胞定位表达载体验证其是否具有核定位功能,利用荧光定量PCR研究其在枳不同组织的表达特性,初步确定SPL9和SPL13在枳生长发育过程中的作用。【方法】利用生物信息学结合RACE技术以枳花器官的cDNA为模板,克隆出SPL9和SPL13基因全长,分别命名Pt-SPL9和Pt-SPL13,大小分别是1519bp和1824bp,在GenBank的登录号分别是FJ502237和FJ502238;构建Pt-SPL9和Pt-SPL13亚细胞定位载体35S-GW-FJ502237/FJ502238-GFP,基因枪转化洋葱表皮细胞,暗培养24h后激光共聚焦显微镜下观察;利用SYBR Green I实时定量RT-PCR方法检测Pt-SPL9和Pt-SPL13在根、茎、叶、花序、花和果等不同组织中的表达。【结果】生物信息学分析表明,Pt-SPL9和Pt-SPL13的cDNA序列中都有microRNA156的识别位点,Pt-SPL9与金鱼草、拟南芥和玉米SPL9的同源性分别为48.9%、42.5%和41.7%;Pt-SPL13与拟南芥SPL13、水稻的SPL16和玉米的TGA1同源性分别为40.8%、38.1%和35.8%。Pt-SPL9和Pt-SPL13与其它植物的SBP一样有着高度保守的序列,即SBP结构域和一个双向核定位信号KRXXXRRRK。亚细胞定位结果表明,Pt-SPL9和Pt-SPL13均定位于细胞核中。SYBR Green I实时定量RT-PCR结果表明,Pt-SPL9和Pt-SPL13在各个器官均有表达,但表达量不同,Pt-SPL9在茎中的表达量最高,在花和叶中的表达量次之,在根、花芽和幼果中的表达量最低;Pt-SPL13在幼果中的表达量最高,在茎和花芽中的表达量相当,其次为叶,在花和根中的表达量很低。【结论】转录因子Pt-SPL9和Pt-SPL13均具有核定位功能,Pt-SPL9和Pt-SPL13对枳的茎和果实的发育可能有着重要作用。  相似文献   

秸秆原料C／N比调节对沼气产气状况的影响   总被引：3，自引：1，他引：2  

张昌爱  刘英  黄萌  袁长波  王艳芹 《山东农业科学》2010,(1):67-70

以玉米秸秆原料为试材,研究了不同碳氮比条件对沼气产气状况的影响。结果表明,碳氮比值为27．0的N1处理,累积产气量、稳定产气周期内的日平均产气量及碳转化效率等指标均达到最大值;随着碳氮比的降低,产气开始时间延迟且产气周期缩短,累积产气量也降低,并且碳转化效率也有所下降。  相似文献   

绿肥翻压年限对植烟土壤微生物量C、N和土壤C、N的影响   总被引：3，自引：0，他引：3  

李正  刘国顺  叶协锋  王永  李娟  杨超  王可 《江西农业学报》2010,22(4):62-65,68

通过3年田间定位试验,研究连年翻压绿肥对植烟土壤微生物量C、N和土壤C、N的影响,结果表明:连年翻压绿肥能够显著提高土壤微生物量C、N含量以及土壤C、N含量,这种提高的趋势随着翻压年限的增加而增加。在烟株整个生育期,植烟土壤翻压绿肥3年后与对照相比,土壤微生物量C、N含量分别提高了30.96%～67.13%、22.96%～145.07%,土壤有机碳、全N、碱解氮含量分别提高了27.84%～53.77%、8.85%～40.95%、11.62%～20.94%,表明翻压绿肥后土壤生物过程活跃,有利于土壤有机物质的转化和烤烟正常生长所需的营养供应。相关分析表明,土壤微生物量C、N在大多数生育时期均与土壤C、N呈显著或极显著正相关。  相似文献   

二化螟越冬幼虫在稻株内的分布及其控制技术研究   总被引：1，自引：0，他引：1  

黄水金  刘剑青  秦文婧  张华满  陈琼 《江西农业学报》2010,22(11)

研究了二化螟越冬代幼虫在不同虫口密度田块和不同受害程度稻株内的分布情况以及农事操作技术措施对越冬二化螟的防治效果。结果表明:二化螟幼虫在水稻植株上的分布数量表现为随着稻株离地面高度的升高而减少,且这种分布趋势与田间的二化螟幼虫密度无关;二化螟幼虫在不同受害程度的稻株上的分布均以5~10 cm的稻株部位最多。在翌年春季,在稻草上越冬的二化螟出蛾率(36.44%)高于在稻桩上越冬的二化螟出蛾率(27.11%),但是在稻桩上越冬二化螟的出蛾数量(138.25)极显著高于在稻草上越冬二化螟的出蛾数量(34.25)。"火烧+堆沤"(冬前在田间对稻草进行焚烧,翌年早春再进行堆沤)处理和旋耕处理对越冬二化螟的防治效果均较好,防效分别为71.17%和66.25%,显著高于单独焚烧稻草或单独堆沤处理的防治效果。  相似文献