苯并芘对猪卵母细胞SHH信号通路影响的研究     

金壬顺  张贞洪  靳鑫  侯少辉  李井春  郭庆 《猪业科学》2021,38(11):88-91

如今空气污染问题仍广泛存在,其中苯并芘（Benzo （a） pyrene,BaP）是一种常见的空气污染物,对人和动物的健康具有严重影响。据报道,BaP可以显著降低卵母细胞IVM （卵母细胞体外成熟）能力,但机制尚不清楚。因此,本研究在猪卵母细胞IVM培养液中添加不同浓度BaP （50 μM、100 μM、200 μM）检测其对卵母细胞IVM能力的影响,并检测此过程SHH信号通路相关基因和蛋白的表达。结果显示50 μM BaP处理组卵母细胞IVM能力显著低于对照组（76.5 vs 68.1,P<0.05）,并随着BaP浓度增加进一步下降。同时,qPCR和免疫荧光染色检测发现,50 μM BaP处理组卵母细胞中SHH信号通路相关基因mRNA和蛋白表达量,均显著低于对照组（P<0.05）。因此,本研究初步证明BaP可能通过破坏SHH信号通路降低猪卵母细胞IVM能力。  相似文献   

Protective Effect of Sulforaphane on Reproductive Function of Male Mice with Cadmium Poisoning     

GAO Feng  HE Jian-bin  ZHANG Yi  LONG Miao  LI Lin  LI Yan-peng  HAN Yang  WANG Xue  JIANG Li-ying  YANG Shu-hua 《中国畜牧兽医》2016,43(2):471-476

The study was aimed to explore the protective effect of sulforaphane (SFN) on the reproductive function of male mice with cadmium poisoning.40 healthy clean grade male Kunming mice were randomly divided into four groups:control group (H₂O),cadmium chloride group (2.3 mg/kg CdCl₂),sulforaphane group (10 mg/kg SFN),sulforaphane + cadmium chloride group (10 mg/kg SFN+2.3 mg/kg CdCl₂),and continuous administration for 10 d,all mice were executed by dislocated cervical vertebra at 2 d after the last administration,and then the pathologic changes of testicular tissues,organ coefficient of testicle and epididymis,sperm quality and concentration of testosterone were tested.Additionally,the contents of GSH and MDA,and the activities of T-SOD in testis were also detected at the same time. Compared with the control group,pathology damages were observed in cadmium chloride group,organ coefficient of testis and epididymis,sperm quality and levels of testosterone extremely significantly decreased (P<0.01),the activities of T-SOD and GSH content were extremely significantly decreased (P<0.01),and the concentration of MDA was extremely significantly enhanced (P<0.01).Compared with the control group,the activity of T-SOD and concentration of GSH in sulforaphane group were significantly increased (P<0.05),and the concentration of MDA was not significant different between the control group and sulforaphane group (P>0.05).While compared with the cadmium chloride group,the sperm motility rate and sperm total count in sulforaphane and cadmium chloride group were extremely significantly increased (P<0.01),the organ coefficient of testicle and epididymis was increased significantly (P<0.05),the concentration of GSH and activity of T-SOD in testicular tissue were extremely significantly increased (P<0.01),and the concentration of MDA was extremely significantly decreased (P<0.01).The results indicated that sulforaphane had the protection effect on reproduction function of male mice with cadmium poisoning.  相似文献   

Effects of Donor Cells Treated with Egg Extracts on the Development Competence of Cloned Embryos     

GUO Qin-qin  FAN Zong-xing  Hao Hai-sheng  LIU Yan  ZHAO Xue-ming  ZHU Hua-bin  DU Wei-hua 《中国畜牧兽医》2016,43(2):477-486

Japanese Balck cattle fetal fibroblasts (JBCFF) were induced with Xenopus leavis egg extracts and somatic cell nuclear transfer (SCNT) was carried out with the reprogrammed JBCFF as donor cells in order to investigate their effects on SCNT efficiency.Three samples of egg extracts were acquired from different Xenopus laevis.The protein contents and kinds in extracts were evaluated with BCA Protein Quantification Kit and SDS-PAGE.Concentration of Digitonin to permeabilize JBCFF was optimized and assessed with PI staining.Reprogrammed cells treated with egg extract were used as donor in SCNT.Additionally the reconstructed embryos were activated with ionomycin+6-DMAP and A23187+6-DMAP to compare their effects on the development competence.The protein contents of extracts samples were 56.2255,64.6570 and 71.2158 μg/mL,respectively,the each extract had the same composition about 40-55 and 70-100 ku.The optimal concentration of Digitonin was 7 μg/mL and the permeabilization rate was 55.44%.After extracts treatment and continuous culture for 6-7 d,JBCFF formed well-defined colony structures.No significant composition difference was found in rates of fusion (92.83% vs 96.04%),cleavage (89.64% vs 89.78%) and blastocyst formation (24.06% vs 23.12%) of cloned embryos when the colony cells and JBCFF without extracts treatment were used as donor cells (P>0.05).Similarly,the two activation methods had no significant effect on the developmental competence of cloned embryos (cleavage rate 92.16% vs 92.28%,blastocyst rate 23.21% vs 24.18%).Conclusively,Xenopus leavis,egg extracts could induce JBCFF reprogramming to a low differentiated state.However donor cells with reprogramming partially could not improve the development of cloned embryos and its mechanism requires further research.  相似文献   

预防用自拟复方中药对大肠杆菌感染早期小鼠免疫功能的影响试验     

《中国兽医杂志》2016,(3)

将96只小鼠随机分为4组,Ⅰ、Ⅱ组生理盐水灌胃,Ⅲ组复方中药灌胃,Ⅳ组黄芪多糖灌胃,连续3 d;试验第4天,Ⅰ组注射生理盐水;Ⅱ、Ⅲ、Ⅳ组小鼠腹腔注射大肠杆菌液。在感染后第3小时、第6小时、第10小时采集小鼠血样及血清,分别测定其血液生理指标(WBC、GRA、LYM及MID)及细胞因子(TNF-α、IL-6、IL-10、IFN-γ)的变化。结果自拟复方中药具有提高小鼠外周血的WBC(尤其是LYM、MID);促进IL-6分泌,抑制TNF-α、IL-10分泌,使Th2向Th1细胞漂移,促进腹腔注射大肠杆菌早期小鼠Th2/Th1细胞亚群恢复平衡的作用。  相似文献   

酒糟培养灵芝提取物对小鼠中枢神经系统的抑制作用观察     

《中国兽医杂志》2016,(9)

为了评价酒糟培养灵芝提取物的药理活性,将小鼠随机分为提取物125 mg/kg体重组、250 mg/kg体重组、500mg/kg体重组,安定组和生理盐水组,观察酒糟培养灵芝的提取物对小鼠自发活动、巴比妥钠阈上剂量致睡眠时间、戊四氮致惊厥的影响。结果显示,提取物的各组、安定组与生理盐水组比较,小鼠的自发活动能极显著减少(P0.01),睡眠时间能显著或极显著延长(P0.05,P0.01),高、中剂量组小鼠的惊厥率显著减少(P0.05)。结果提示,酒糟培养灵芝的提取物对小鼠中枢神经系统具有一定的抑制作用。  相似文献   

羊体外胚胎高效生产技术研究现状     

齐雅天  房晓欢  李俊杰 《黑龙江动物繁殖》2023,(2):7-12

体外胚胎生产技术作为胚胎工程技术的基础,在提高繁殖潜力、加速良种繁育等多方面具有重要意义,包括卵母细胞采集、体外成熟、体外受精、体外胚胎培养等技术。文章主要介绍羊体外胚胎高效生产技术研究现状,分析卵母细胞体外成熟、体外受精、体外胚胎培养影响因素,并提出了羊卵泡的预测标记、卵母细胞新型质检手段、模拟羊体内环境的培养体系,以期为羊卵母细胞体外培养技术的研究及在胚胎高效生产中的应用提供参考。  相似文献   

感染小鼠肠道内猪痢疾密螺旋体的定位研究     

程国富 《华中农业大学学报》1987,(4)

用过氧化物酶-抗过氧化物酶法(PAP)对感染猪痢疾密螺旋体的小鼠肠道组织切片进行染色观察。发现特异的酶染颗粒主要分布于盲肠,其次是结肠前段和后段。在感染的早期,主要分布于肠腺的深部,以后有逐渐向肠腔面转移的趋势。在小肠和肠细胞内未见有特异性的酶染颗粒。通过与常规的镀银法比较,作者认为PAP法更为灵敏、特异,不失为一种有效的猪痢疾病的研究方法。  相似文献   

慢性束缚应激对小鼠攻击行为的影响及机制探讨     

《南京农业大学学报》2021,44(5)

[目的]研究慢性束缚应激对小鼠攻击行为的影响及其机制。[方法]选取52只7周龄C57BL/6J雄性小鼠,随机分为对照组(control group, Con)和慢性束缚应激组(chronic restraint stress group, RS)。另选取7周龄24只小鼠(公母各半),配对饲养,同样分为2组(对照组和慢性束缚应激组),其中雄性小鼠作为驻留者用于攻击行为评测,雌性小鼠仅用作陪伴。再选取6周龄雄性小鼠12只作为入侵者用于攻击行为评测。慢性束缚应激组小鼠每天束缚6 h(09:00—15:00),连续处理7 d,应激小鼠处理期间对照组小鼠禁食禁水,而雌性小鼠和入侵者小鼠未做任何处理。记录试验起始及结束时小鼠体重,检测小鼠攻击行为、血浆和下丘脑神经递质水平、下丘脑攻击行为相关基因及蛋白表达量。[结果]与对照组相比,慢性束缚应激极显著抑制小鼠生长(P0.01),显著降低小鼠攻击潜伏期时间(P0.05),显著增加小鼠总攻击次数(P0.05),显著上调小鼠下丘脑囊泡单胺类转运体2、5-羟色胺受体1b、乙醛脱氢酶1a1、多巴胺脱羧酶、多巴胺受体2及多巴胺转运体mRNA水平(P0.05或P0.01),极显著下调小鼠下丘脑DAT蛋白表达量(P0.01)。[结论]慢性束缚应激可降低小鼠生长性能,增加小鼠攻击行为,攻击行为的改变可能与下丘脑DAT蛋白低表达密切相关。  相似文献   

虾原肌球蛋白诱导小鼠过敏性哮喘气道重塑模型的研究     

严莹  邓晋芳  解心仪  陈静茹  张顺婷  何振鹏  方蕾 《扬州大学学报(农业与生命科学版)》2021,42(1):47-51

以虾原肌球蛋白每周腹腔注射致敏小鼠1次,连续3周,再持续进行6周滴鼻激发诱导小鼠发生气道重塑.采用ELISA法检测血清总IgE水平,测定肺气道阻力,HE染色观察肺组织形态学变化,PAS染色观察气道上皮细胞黏液分泌状态,Masson三色法检测胶原纤维增生,免疫组织化学法检测肺组织a-平滑肌肌动蛋白的表达,以探索建立一种以...  相似文献   

结石通片对小鼠的急性毒性试验及其对乙二酰胺致大鼠肾结石的作用研究     

黎伟华  金学琴  吴璟 《畜牧与饲料科学》2021,42(2):7-10

[目的]研究结石通片对小鼠的急性毒性作用,对健康大鼠的利尿作用,以及对乙二酰胺致大鼠肾结石的调节作用.[方法]取ICR小鼠40只,雌、雄各半,随机分为对照组与给药组,每组20只,观察结石通片给药前后小鼠的体重变化和死亡情况;另取SD大鼠36只,雌、雄各半,分为对照组、低剂量组(给予5％结石通片溶液)和高剂量组(给予10...  相似文献