Citronella oil‐loaded electrospun micro/nanofibrous matrices as sustained repellency systems for the Asian tiger mosquito Aedes albopictus     

Konstantina Iliou  Stefanos Kikionis  Panos V Petrakis  Efstathia Ioannou  Vassilios Roussis 《Pest management science》2019,75(8):2142-2147

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Induced novel psbA mutation (Ala251 to Thr) in higher plants confers resistance to PSII inhibitor metribuzin in Lens culinaris     

Larn S McMurray  Christopher Preston  Albert Vandenberg  Dili Mao  Kirstin E Bett  Jeffrey G Paull 《Pest management science》2019,75(6):1564-1570

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Enhanced metabolism causes reduced flufenacet sensitivity in black‐grass (Alopecurus myosuroides Huds.) field populations     

Rebecka Dücker  Peter Zllner  Evlampia Parcharidou  Susanne Ries  Lothar Lorentz  Roland Beffa 《Pest management science》2019,75(11):2996-3004

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杀虫真菌与苏云金芽胞杆菌对草地贪夜蛾的联合室内杀虫活性研究          下载免费PDF全文

彭国雄  张淑玲  张维  夏玉先 《中国生物防治学报》2019,35(5):735-740

草地贪夜蛾Spodoptera frugiperda（J.E.Smith）是一种迁飞性重大害虫。为了弄清不同微生物联合使用对草地贪夜蛾的生防潜力,在室内条件下采用直接喷雾方法,测定了金龟子绿僵菌Metarhizium anisopliae CQMa421、球孢白僵菌Beauveria bassiana ZJU435和苏云金芽胞杆菌（Baci1lus thuringiensis,Bt）G033A两两之间对草地贪夜蛾幼虫的室内联合杀虫活性。结果表明：金龟子绿僵菌CQMa421与球孢白僵菌ZJU435悬浮液联合接种10 d后,1～3龄草地贪夜蛾幼虫校正死亡率分别为65.6%、58.8%和35.4%,都显著高于CQMa421和ZJU435单剂处理组。CQMa421与Bt联合接种5 d后,2龄草地贪夜蛾幼虫校正死亡率为82.6%,显著高于CQMa421（21.1%）和Bt（65.5%）单剂处理;ZJU435与Bt联合接种5 d后,2龄和4龄草地贪夜蛾幼虫校正死亡率为80.4%和68.6%,显著高于Bt和ZJU435单剂处理组。上述试验结果表明：不同杀虫真菌联合使用、杀虫真菌与Bt联合使用能有效提高对草地贪夜蛾幼虫的杀虫活性,为田间利用不同微生物农药有效防控草地贪夜蛾提供了依据。  相似文献   

苏云金芽胞杆菌杀虫晶体蛋白Cry1D新基因的克隆与特性          下载免费PDF全文

余宗兰  贺利业  孙宏伟  李平  郑爱萍 《中国生物防治学报》2019,35(2):288-294

为扩充鳞翅目害虫杀虫基因资源,本研究从苏云金芽胞杆菌BN23-5中克隆得到一个新的cry基因,并对其进行鉴定和分析。该基因为一个完整的cry1D基因,全长3501 bp,编码1166个氨基酸残基。该氨基酸序列是一个新的Cry氨基酸序列,与Cry1Db1的同源性最高,为86%,命名为Cry1Dd1（登录号为KJ728844）。将该基因插入穿梭表达载体pSTK中,转入BT无晶体突变株HD73^-中进行表达。结果表明,cry1Dd1基因能在BT无晶体突变株中表达,并形成菱形伴孢晶体。SDS-PAGE验证其分子量为132.2 kD,与预测的大小相符。生物活性测定表明,Cry1Dd1晶体蛋白对小菜蛾的幼虫具有杀虫活性,LC₅₀为13.1 μg/mL;能明显抑制甜菜夜蛾幼虫的生长;但对棉铃虫幼虫没有杀虫活性。对cry1Dd1基因序列进行分析,cry1Dd1包含8个block保守区域,这和目前其他的cry基因相似;Cry1Dd1蛋白的活性区域为N端的37~593位氨基酸残基。  相似文献   

利用CRISPR/Ca9技术靶向编辑芥蓝BoaZDS     

郑爱红  张芬  江敏  袁巧  江雷雨  陈清  汤浩茹  孙勃 《园艺学报》2019,46(1):57-64

以芥蓝(Brassicaoleraceavar.alboglabra)为材料,以ζ–胡萝卜素脱氢酶(ζ-Carotene desaturase,ZDS)基因为目标基因,建立其CRISPR/Cas9基因组编辑体系。在BoaZDS的编码区近5′端选择靶位点,构建了CRISPR/Cas9表达载体,通过农杆菌介导的遗传转化方法获得了19个芥蓝转基因阳性植株,Sanger测序分析发现其中13株成功突变,CRISPR/Cas9载体在芥蓝上的突变效率为68.42%,且所有突变植株均表现出明显的白化表型。  相似文献   

利用CRISPR/Cas9敲除葡萄VviPDS1基因的研究     

郭晔  万东艳  柴壮壮  王跃进  文颖强 《园艺学报》2019,46(4):623-634

选择葡萄八氢番茄红素脱氢酶(Phytoene desaturase)基因VviPDS1为靶标,利用CRISPR/Cas9系统构建基因敲除载体,瞬时转化葡萄叶片原生质体,检测到不同类型的突变。通过农杆菌介导转化‘无核白’葡萄胚性愈伤组织,筛选获得卡那霉素抗性植株71株。经PCR鉴定,其中53株为阳性植株,阳性率为74.64%。测序结果表明,共有20株在靶点发生不同类型的突变,编辑效率为37.74%;其中9株产生了双等位基因突变。对其进行氨基酸序列预测,在第202位氨基酸之后发生了不同程度的变异。利用CRISPR/Cas9系统敲除VviPDS1获得的突变体植株呈现整体矮化,其叶片出现不同程度白化。表明CRISPR/Cas9系统可以通过细胞中的瞬时或稳定表达进行基因编辑,可以实现在葡萄编辑植株中产生纯合敲除。  相似文献   

Effect of miRNA-33 on ABCA1/ABCG1 expression in RAW264.7 macrophage-derived foam cells after Hcy treatment     

DAI Pei  GAO Fen  GAO Hong-wei  WANG Yuan  FENG Gao-jie  ZHANG Qin-feng  BAI Rui  QIN Wei-wei  LI Hong  SONG Xiao-su 《园艺学报》2019,35(2):212-217

AIM:To study whether homocysteine (Hcy) inhibits the expression of ATP-binding cassette transporter A1 (ABCA1) and ATP-binding cassette transporter G1 (ABCG1) by microRNA-33 (miRNA-33) signaling, and reduces the efficiency of reverse cholesterol transport (RCT).METHODS:RAW264.7 macrophages were induced by oxidized low-density lipoprotein (ox-LDL) to establish foam cell model. Oil red O staining was used to determine whether the model was established successfully. miRNA-33 mimics and miRNA-33 inhibitor were transfected into the cells by Lipofectamine 2000, and the cells were exposed to Hcy at concentration of 5 mmol/L for 24 h. The intracellular lipid droplets were observed by Oil red O staining. The expression of ABCA1 and ABCG1 at mRNA and protein levels was determined by real-time PCR and Western blot. The cellular cholesterol content was analyzed by HPLC, and effluent rate of cholesterol was detected by the method of liquid scintillation counting.RESULTS:Compared with blank control group, the lipid content in miRNA-33 mimics group was increased, and the expression of ABCA1 and ABCG1 at mRNA and protein levels was decreased (P<0.05). The intracellular cholesterol content was increased gradually (P<0.05), and the cellular cholesterol efflux rate was gradually decreased (P<0.05) in miRNA-33 mimics group. Compared with blank control group, the testing results in miRNA-33 inhibitor group were the opposition of those in miRNA-33 mimics group (P<0.05). No diffe-rence of the above indexes among blank control group, miRNA-33 mimics-NC group and miRNA-33 inhibitor-NC group was observed.CONCLUSION:Hcy inhibits the mRNA and protein expression of ABCA1 and ABCG1 through miRNA-33 signaling, and reduces the efficiency of RCT in RAW264.7 macrophage-derived foam cells.  相似文献   

CUDC-907 induces DNA damage and autophagy in glioma cells     

JIAO Peng  HUANG Zhen-zhou  ZHANG Xiao-jing  LONG Yan-jun  LI Zhao-jun  WANG Feng-ze 《园艺学报》2019,35(2):260-266

AIM:To investigate the effect of CUDC-907, a dual histone deacetylase (HDAC) and phosphatidylinositol 3-kinase (PI3K) inhibitor, on the DNA damage, cell cycle distribution and autophagy in human glioma U251 cells. METHODS:U251 cells were treated with CUDC-907 of different concentrations, and the cell viability was detected by MTT assay. The quantitative γ-H2AX foci were determined by laser scanning confocal microscopy. The cell cycle distribution of U251 cells was examined by flow cytometry. The protein expression was determined by Western blot analysis. RESULTS:CUDC-907 inhibited the cell viability and the phosphorylation of Akt and p70 ribosomal protein S6 kinase (p70s6K) in the U251 cells (P<0.05). In CUDC-907-treated cells, the number of γ-H2AX foci and protein expression of γ-H2AX were increased significantly (P<0.05). CUDC-907 also induced cell arrest in the G₂/M phase by up-regulating the expression of p21, and inhibiting the protein level of cyclin B1 and the phosphorylation of cell division cycle protein 2 (Cdc2). In addition, CUDC-907 triggered cell autophagy, and inhibition of autophagy increased CUDC-907-induced DNA damage of U251 cells. CONCLUSION:CUDC-907 significantly inhibits PI3K/Akt signaling pathway, induces DNA damage and arrests cell cycle in G₂/M phase. Blockage of autophagy promotes CUDC-907-induced DNA damage of U251 cells.  相似文献   

Effect of FGFR1 expression knock-down on biological characteristics of infantile hemangioma endothelial cells     

XU Nan  XU Jie  LI Han  QIAN Li-juan  LI Zhong-tang 《园艺学报》2019,35(3):418-423

AIM: To study the effect of fibroblast growth factor receptor 1 (FGFR1) expression knock-down on the viability, apoptosis, invasion and migration of infantile hemangioma endothelial cells (HemECs). METHODS: FGFR1 was down-regulated by FGFR1 small interfering RNA (si-FGFR1) transfection. The viability of the cells was measured by CCK-8 assay. The apoptotic rate was analyzed by flow cytometry and the invasion and migration abilities were determined by Transwell assay. The protein levels of phosphatidylinositol 3-kinase (PI3K), protein kinase B (AKT), and phosphorylated AKT (p-AKT) were examined by Western blot. RESULTS: Transfection of si-FGFR1 into HemECs had significant effects on inhibiting cell viability (P<0.05), promoting apoptosis (P<0.05), and decreasing cell invasion and migration abilities (P<0.05). The results of Western blot showed that knockdown of FGFR1 gene expression in the cells reduced the protein levels of PI3K and p-AKT (P<0.05), and had no significant effect on AKT protein level. CONCLUSION: Knock-down of FGFR1 expression changes the biological characteristics of endothelial cells in infantile hemangiomas by regulating PI3K/AKT signaling pathway.  相似文献