Development of a cost‐effective pyrosequencing approach for SNP genotyping in barley     

Cristina Silvar  Dragan Perovic  Ana M. Casas  Ernesto Igartua  Frank Ordon 《Plant Breeding》2011,130(3):394-397

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辽宁新品系绒山羊Hoxc-13基因的结构和分析     

丁琰  金梅  向飞  王喆 《安徽农学通报》2008,14(22)

本文论述了在研究辽宁新品系绒山羊皮肤上调控绒细度基因时,通过RT-PCR和RACE方法,在皮肤中获得了Hoxc-13启动子1 367bp。此外,本文描述了Hoxc-13基因的分离和特性,并进行比较分析,在控制毛发角蛋白转录方面,Hoxc-13基因在皮肤中可能还有其他的重要功能。  相似文献   

Inv(13)(p12 q14)一家系报道     

李露霞  付建华 《新疆农业大学学报》1993,(4)

先证者女汉族24岁,身高1.61米.体重60.1公斤。因连续生两胎畸形儿夭折就诊。第一胎为足月顺产,唇裂、腭裂.双脚各为6趾,50天时因呼吸道感染而死亡。第二胎.足月顺产,体征似第一胎,生下两天死亡。外周血染色体检查,分别计数50个细胞,G 显带分析20个核型均为46,xx.Inv(13)(p12 q14)臂间倒位.患者父亲及其兄长核型与患者相同。其丈夫体征与核型均正常。其姐、妹及母亲因居住内地未查,患者哥及姐所生子女表形均无异常。  相似文献   

分子标记选择小麦抗白粉病基因Pm4b、Pm13和Pm21聚合体   总被引：43，自引：2，他引：43  

张增艳  陈孝  张超  辛志勇  陈新民 《中国农业科学》2002,35(7):789-793

 培育多个抗病基因聚合的小麦品种是提高其抗病广谱性和持久性的有效途径之一。利用小麦抗白粉病基因Pm4、Pm13、Pm2 1的特异PCR标记 ,对含有Pm4b、Pm13、Pm2 1的小麦品系复合杂交F2 代 4 0个植株进行检测 ,从中选择到Pm4b +Pm13+Pm2 13个基因聚合的抗病植株 11个 ,检测、选择到Pm4b +Pm13、Pm4b +Pm2 1、Pm13+Pm2 12个基因聚合的抗病植株 19个 ,为持久、广谱抗病小麦育种奠定了基础。研究还表明 ,3个独立的显性抗病基因在F2 代分离群体中的分离比例基本符合孟德尔独立分配定律 ,在小麦背景下的遗传稳定性 ,与该基因供体和普通小麦的亲缘关系密切相关 ,亲缘关系越远 ,丢失的概率越大。因此 ,在育种过程中对外源基因鉴定和跟踪非常必要  相似文献   

~(13)C-核磁共振谱在鉴定天然产物结构中的应用     

张德志  张伟森 《吉林林学院学报》1991,(3)

本文参考有关文献资料,结合应用核磁共振~(13)C 谱鉴定两个四环二萜类化合物结构的工作,对一些天然化合物,如具有含氧基团的环己烷体系的化合物中,由于其含氧基团的取代情况不同而引起其核磁共振~(13)C 谱各相关碳信号呈现出的规律性变化,进行了较详细的讨论。  相似文献   

商丘市夏玉米高产（13 500 kg/hm²以上）栽培技术     

皇飞  郭振升  李勤  李广勤  张慎举 《陕西农业科学》2023,69(3):55-59

根据商丘市夏玉米高产栽培科技攻关的实践,提出了商丘市夏玉米高产(13 500 kg/hm²以上)栽培的技术路线和土壤肥力、全生育期施肥量、熟相、产量结构等指标,集成了商丘市夏玉米高产(13 500 kg/hm²以上)栽培技术,可为豫东平原及生态条件类似区夏玉米高产创建和高产攻关提供参考。  相似文献   

六种限制性内切酶产生的二花脸大约克夏猪的DNA指纹图谱   总被引：7，自引：1，他引：7  

丁波  姜志华 《南京农业大学学报》1994,17(3):75-80

用噬菌体Ｍ１３ｍｐ１８单链ＤＮＡ作探针，对二花脸和大约克夏猪进行了ＤＮＡ指纹的研究。采用六种限制性内切酶获得了高分辨率的、具有个体特异性的ＤＮＡ指纹图谱。这六种限制性内切酶是：ＨｉｎｆⅠ，ＨａｅⅢ，ＥｃｏＲⅠ，ＢａｎⅠ，ＴａｑⅠ和ＰｓｔⅠ。不同酶消化产生的图谱带数有差异，并有品种特征带趋向。  相似文献   

Protected wading bird species threaten relict centenarian cork oaks in a Mediterranean Biosphere Reserve: A conservation management conflict     

Luis V. García  Cristina Ramo  Cristina Aponte  Adela Moreno  María T. Domínguez  Lorena Gómez-Aparicio  Ramón Redondo  Teodoro Marañón 《Biological conservation》2011,(2):764-771

Conservation management conflicts frequently arise when an overpopulation of a protected organism has negative effects on other valuable elements in the same ecosystem. We studied the interactions between a colony of protected tree-nesting wading birds and a remnant population of centenarian cork oaks that was part of the formerly dominant forests in the Doñana Biological Reserve (SW Spain). A significant increase in the tree mortality rates has been recorded in areas that are yearly influenced by the bird colony.We analysed a cohort of surviving trees using a gradient of nesting bird influence. Tree-nesting history, bird isotopic signature (δ¹⁵N), tree health-related parameters (defoliation, δ¹³C and leaf surface coverage by faeces) and several soil variables were evaluated. Bird influence was related to increased soil salinity. This increase correlated to increased water-use efficiency for the leaves and to crown defoliation, suggesting that the heavily occupied trees are under higher stress and in poorer health condition than the unoccupied ones. We tested structural equations models (SEM) that were based on hypothesised bird effects on the health of the trees. Soil-mediated effects of the nesting birds best explained the symptoms of the declining health of the trees, whereas the percent of leaves’ surface that was covered by faeces did not improve the fitted SEM model.For the reserve’s managers, a challenging trade-off exists between preserving the relict trees, which have a high genetic diversity and a key ecological role in these savannah-like ecosystems, and maintaining the current nesting area for these protected, but expanding, wading birds.  相似文献   

Microbial community composition and carbon cycling within soil microenvironments of conventional, low-input, and organic cropping systems   总被引：1，自引：0，他引：1  

Kong AY  Scow KM  Córdova-Kreylos AL  Holmes WE  Six J 《Soil biology & biochemistry》2011,43(1):20-30

This study coupled stable isotope probing with phospholipid fatty acid analysis (¹³C-PLFA) to describe the role of microbial community composition in the short-term processing (i.e., C incorporation into microbial biomass and/or deposition or respiration of C) of root- versus residue-C and, ultimately, in long-term C sequestration in conventional (annual synthetic fertilizer applications), low-input (synthetic fertilizer and cover crop applied in alternating years), and organic (annual composted manure and cover crop additions) maize-tomato (Zea mays - Lycopersicum esculentum) cropping systems. During the maize growing season, we traced ¹³C-labeled hairy vetch (Vicia dasycarpa) roots and residues into PLFAs extracted from soil microaggregates (53-250 μm) and silt-and-clay (<53 μm) particles. Total PLFA biomass was greatest in the organic (41.4 nmol g⁻¹ soil) and similar between the conventional and low-input systems (31.0 and 30.1 nmol g⁻¹ soil, respectively), with Gram-positive bacterial PLFA dominating the microbial communities in all systems. Although total PLFA-C derived from roots was over four times greater than from residues, relative distributions (mol%) of root- and residue-derived C into the microbial communities were not different among the three cropping systems. Additionally, neither the PLFA profiles nor the amount of root- and residue-C incorporation into the PLFAs of the microaggregates were consistently different when compared with the silt-and-clay particles. More fungal PLFA-C was measured, however, in microaggregates compared with silt-and-clay. The lack of differences between the mol% within the microbial communities of the cropping systems and between the PLFA-C in the microaggregates and the silt-and-clay may have been due to (i) insufficient differences in quality between roots and residues and/or (ii) the high N availability in these N-fertilized cropping systems that augmented the abilities of the microbial communities to process a wide range of substrate qualities. The main implications of this study are that (i) the greater short-term microbial processing of root- than residue-C can be a mechanistic explanation for the higher relative retention of root- over residue-C, but microbial community composition did not influence long-term C sequestration trends in the three cropping systems and (ii) in spite of the similarity between the microbial community profiles of the microaggregates and the silt-and-clay, more C was processed in the microaggregates by fungi, suggesting that the microaggregate is a relatively unique microenvironment for fungal activity.  相似文献   

Carbon and nitrogen degradation on molecular scale of grass-derived pyrogenic organic material during 28 months of incubation in soil     

André Hilscher  Heike Knicker 《Soil biology & biochemistry》2011,43(2):261-270

The present study focuses on the microbial recalcitrance of pyrogenic organic material (PyOM) on a molecular scale. We performed microcosm incubation experiments using ¹³C- and ¹⁵N-enriched grass-derived PyOM mixed with a sub soil material taken from a Haplic Cambisol. Solid-state ¹³C and ¹⁵N NMR studies were conducted to elucidate the humification processes at different stages of PyOM degradation. The chemical structure of the remaining PyOM after incubation was clearly different from the initial pyrogenic material. The proportion of O-containing functional groups was increased, whereas that of aryl C and of N-containing heterocyclic structures had decreased, probably due to mineralisation and conversion to other C and N groups. After 20 months of incubation the aryl C loss reached up to 40% of the initial amount and up to 29% of the remaining PyOM C was assigned to carboxyl/carbonyl C and O-aryl C. These reactions alter the chemical and physical properties of the char residue and make it more available for further microbial attack but also for adsorption processes. Our study presents direct evidence for the degradation of N-heterocyclic domains in charred plant remains adding new aspects to the understanding of the N cycling in fire-affected ecosystems.  相似文献