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41.
We investigated nitrous oxide (N2O) emission from an irrigated rice field over two years to evaluate the management of nitrogenous fertiliser and its effect on reducing emissions. Four forms of nitrogenous fertilisers: NPK at the recommended application rate, starch–urea matrix (SUM) + PK, neem‐coated urea + PK and urea alone (urea without coating) were used. Gas samples were collected from the field at weekly intervals with the static chamber technique. N2O emissions from different treatments ranged from 11.58 to 215.81 N2O‐N μg/m2/h, and seasonal N2O emissions from 2.83 to 3.89 kg N2O‐N/ha. Compared with other fertilisers, N2O emissions were greatest after the application of the conventional NPK fertiliser. Moreover, SUM + PK reduced total N2O emissions by 22.33% (< 0.05) compared with NPK during the rice‐growing period (< 0.05). The results indicate a strong correlation between N2O emissions and soil organic carbon, nitrate, ammonium, above‐ and below‐ground plant biomass and photosynthesis (< 0.05). The application of SUM + PK in rice fields is suitable as a means of reducing N2O emissions without affecting grain production.  相似文献   
42.
试验采用密闭箱技术对呼伦贝尔地区种植的苜蓿人工草地进行碳通量研究,采用反硝化-分解作用模型(DNDC)进行模拟。试验结果表明:对苜蓿人工草地CO2通量24h连续观测发现,CO2通量全天变化的平均值(238.15mg/(m2·h)与11∶00(245.32mg/(m2·h)的通量变化值接近,试验从早上9∶00~11∶00进行温室气体的采集方法合理、可行;在整个生长季变化表明,7、8月CO2通量变化较显著(P0.05),且受到降水的影响较大,伴随降水的发生,CO2通量会发生不同幅度的变化,土壤温度和含水量对CO2通量有一定的影响(r0.45,P0.05);通过DNDC模型的模拟,能较好的反映苜蓿人工草地CO2通量的变化情况,但模拟结果数值普遍有些偏高。  相似文献   
43.
Rather than a human-centric, the basic strategy of achieving Sustainable Development Goals must be focused on restoring and sustaining planetary processes. The urgency of meeting the demands of the humanity must be reconciled with the necessity of enhancing the environment. Increasing and restoring soil organic matter content of the degraded and depleted soils is critical to strengthening planetary processes.  相似文献   
44.
Although the effects of cover crops (CC) on various soil parameters have been fully investigated, less is known about the impacts at different stages in CC cultivation. The objective of this study was to quantify the influence of CC cultivation stages and residue placement on aggregates and microbial carbon (Cmic). Additionally, the influence of residue location and crop species on CO2 emissions and leached mineralized nitrogen (Nmin) during the plant degradation period was also investigated. Within an incubation experiment, four CC species were sown in soil columns, with additional columns being kept plant‐free. After plant growth, the columns were frozen (as occurs in winter under field conditions) and then incubated with the plant material either incorporated or surface‐applied. With CC, concentrations of large and medium macroaggregates were twice that of the fallow, confirming positive effects of root growth. Freezing led to a decrease in these aggregate size classes. In the subsequent incubation, the large macroaggregates decreased far more in the samples with CC than in the fallow, leading to similar aggregate size distributions. No difference in Cmic concentration was found among the CC cultivation stages. CO2 emissions were roughly equivalent to the carbon amounts added as plant residues. Comparison of columns with incorporated or surface‐applied residues indicated no consistent pattern of aggregate distribution, CO2 emission or Cmic and Nmin concentrations. Our results suggest that positive effects of CC cultivation are only short term and that a large amount of organic material in the soil could have a greater influence than CC cultivation.  相似文献   
45.
研究确立了乙腈为分散萃取溶剂,以羧基化双壁碳纳米管(DWCNTs-COOH)和石墨化碳黑(GCB)为基质分散材料的菠菜(Spinacia oleracea L.)样品中噻虫胺残留基质分散萃取前处理方法,建立并优化了乙腈等度洗脱的HPLC残留样品外标定量方法。结果表明,优化色谱条件下,噻虫胺标准样品的色谱保留时间为6.258 min,在0.05~100 mg/L范围内与对应色谱峰积分面积线性响应良好,回归方程为y=4.948 2x-1.316 9(R~2=0.999 7),噻虫胺在菠菜样品中0.1、0.5和5.0 mg/kg 3个水平的添加回收率均在85%以上,各添加水平的相对标准偏差均小于7%,该色谱条件下仪器检出限为0.052 7μg/L,方法的最低检测量为0.015 mg/kg。表明该残留样本前处理方法和样品检测方法简便、高效、经济、可靠,可满足噻虫胺在菠菜中的残留定量检测要求。  相似文献   
46.
李荣娟  黄承烈 《安徽农业科学》2014,(26):9132-9134,9137
加强节能减排,实现低碳发展,不仅是国家“十二五”规划纲要所提出的约束性目标,更是促进经济提质增效升级的必经途径.目前,我国县域经济仍以“高投入、高消耗、高排放”为主要特征,转变经济发展方式已成为县级政府所面临的迫切任务.为此,必须依据低碳发展理念和《2014 ~2015年节能减排低碳发展行动方案》的具体要求,培育有利于经济发展方式转变的要素基础和制度基础,加快推进产业结构的优化和升级,深化经济管理体制改革,不断进行制度创新,实施有利于科技进步的政策,完善环境管理制度,实现县域经济又好又快发展.  相似文献   
47.
由于DNA重组技术和蛋白组技术的发展与成熟,外源基因表达技术备受关注。其在生物、食品、工业以及医学领域发挥着举足轻重的作用。以原核生物细胞及真核生物细胞作为表达系统,进行目的基因序列的表达以生产蛋白疫苗、核酸疫苗和生物酶制剂等是近年来发酵工业的新型领域。原核和真核表达系统是近年来研究和应用最广泛和最成熟的外源基因表达系统。对近年来关于大肠杆菌表达系统和酵母表达系统的研究进展进行了综述。  相似文献   
48.
Biochar addition can expand soil organic carbon (SOC) stock and has potential ability in mitigating climate change. Also, some incubation experiments have shown that biochar can increase soil inorganic carbon (SIC) contents. However, there is no direct evidence for this from the field experiment. In order to make up the sparseness of available data resulting from the long‐term effect of biochar amendment on soil carbon fractions, here we detected the contents and stocks of the bulk SIC and SOC fractions based on a 10‐year field experiment of consecutive biochar application in Shandong Province, China. There are three biochar treatments as no‐biochar (control), and biochar application at 4.5 Mg ha?1 year?1 (B4.5) and 9.0 Mg ha?1 year?1 (B9.0), respectively. The results showed that biochar application significantly enhanced SIC content (3.2%–24.3%), >53 μm particulate organic carbon content (POC, 38.2%–166.2%) and total soil organic carbon content (15.8%–82.2%), compared with the no‐biochar control. However, <53 μm silt–clay‐associated organic carbon (SCOC) content was significantly decreased (14%–27%) under the B9.0 treatment. Our study provides the direct field evidence that SIC contributed to carbon sequestration after the biochar application, and indicates that the applied biochar was allocated mainly in POC fraction. Further, the decreased SCOC and increased microbial biomass carbon contents observed in field suggest that the biochar application might exert a positive priming effect on native soil organic carbon.  相似文献   
49.
The response of the soil food web structure to soil quality changes during long-term anthropogenic disturbance due to farming practices has not been well studied. We evaluated the effects of three tillage systems: moldboard plow/rotary harrow (MP), rotary cultivator (RC), and no-tillage (NT), three winter cover-crop types (fallow, FL; rye, RY; and hairy vetch, HV), and two nitrogen fertilization rates (0 and 100 kg N ha−1 for upland rice, and 0 and 20 kg N ha−1 for soybean production) on changes in nematode community structure. Sixty-nine taxa were counted, total nematode abundance (ALL), bacterial feeders (BAC), predators (PRD), omnivores (OMN), and obligatory root feeders (ORF) were more abundant in NT than in MP and RC, but fungal feeders and facultative root feeders (FFR) were more abundant in RC than in NT and MP. Cover crop also influenced nematode community structure; rye and hairy vetch were always higher in ALL, BAC, FFR, ORF, and OMN than fallow. Seasonal changes in nematode community structure were also significant; in particular, as soil carbon increased, nematode abundance also increased. The relationship between nematode indices and soil carbon was significant only in NT, but not in MP and RC. In NT, with increasing soil carbon, enrichment index and structure index (SI) were positive and significant and channel index was negative. Bulk density was significantly negatively correlated with FFR and ORF. Seasonal difference in nematode community between summer and autumn was larger in an upland rice rotation than in a soybean rotation. Over the nine-year experiment, SI increased not only in NT but also in MP and RC, suggesting that repeated similar tillage inversions in agroecosystems may develop nematode community structures adapted to specific soil environmental conditions. Because NT showed the highest values of both SI and soil carbon, the increase of soil carbon in NT is expected to have a great impact on developing a more diverse nematode community structure.  相似文献   
50.
In order to quantify the influence of land use systems on the level of soil organic matter (SOM) to develop recommendations, long-term field studies are essential. Based on a crop rotation experiment which commenced in 1970, this paper investigated the impact of crop rotations involving increased proportions of sugar beet on SOM content. To this end, soil samples were taken in 2010 and 2012 from the following crop rotation sequences: sugar beet–sugar beet–winter wheat–winter wheat (SB–SB–WW–WW = 50%), sugar beet–sugar beet–sugar beet–winter wheat (SB–SB–SB–WW = 75%), sugar beet–grain maize (SB–GM = 50%) and sugar beet-monoculture (SB = 100%); these were analysed in terms of total organic carbon (TOC) and microbial biomass carbon (MBC) content, MBC/TOC ratio and the TOC stocks per hectare. In addition, humus balances were created (using the software REPRO, reference period 12 years) in order to calculate how well the soil was supplied with organic matter. In the field experiment, harvest by-products (WW and GM straw as well as SB leaves) were removed. After 41 years, no statistically significant differences were measured between the crop rotations for the parameters TOC, MBC, MBC/TOC ratio and the TOC stock per hectare. However, the calculated humus balance was significantly affected by the crop rotation. The calculated humus balance became increasingly negative in the order SB–SB–WW–WW, SB–SB–SB–WW, SB monoculture and SB–GM, and correlated with the soil parameters. The calculated humus balances for the reference period did not reflect the actual demand for organic matter by the crop rotations, but instead overestimated it.  相似文献   
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