圆叶决明添加量对红壤可溶性氮及酶活性的影响     

钟珍梅  杨庆  游小凤  邢世和 《草地学报》2022,30(3):622-630

为了探明圆叶决明(Chamaecrista rotundifolia)降解后红壤可溶性氮及氮水解酶活性的变化规律,本研究采用室内好气恒湿培养法,研究占红壤质量0.5%(T1),1%(T2)和2%(T3)的圆叶决明添加至红壤中,培养7~88d内红壤硝态氮(NO^-₃-N)、铵态氮(NH⁺₄-N)和可溶性有机氮(Soluble organic nitrogen,SON)及脲酶、蛋白酶和天冬酰胺酶的变化。结果表明：添加圆叶决明后,红壤NO^-₃-N和NH⁺₄-N含量在培养前期降低,培养中期增加;而整个培养期SON含量及脲酶、蛋白酶和天冬酰胺酶活性均增加,且圆叶决明添加量越大,效果越显著。NO^-₃-N和SON含量及脲酶、蛋白酶和天冬酰胺酶活性可用2次或3次函数方程拟合;而NH⁺₄-N含量可用线性函数拟合。氮水解酶与NO^-₃-N负相关,与NH⁺₄-N和SON正相关,且相关性从大到小的顺序为蛋白酶>天冬酰胺酶>脲酶。综上,添加圆叶决明提高了红壤供氮水平和红壤氮转化能力。  相似文献   

杨树苗木叶硝酸还原酶活力的初步研究   总被引：3，自引：0，他引：3       下载免费PDF全文

刘雅荣  王世绩  周国璋  苏梦云 《林业科学研究》1988,(3):340-344

硝酸还原酶是植物体内氮素代谢的关键酶。植物从外界环境中吸收的硝酸盐需要经过一系列酶促反应才能转化成氨基酸和蛋白质,以供生长需要。硝酸还原酶是这个代谢过程中的第一个酶,也是个限速酶,其活力的高低与植物同化NO_3~--N的能力和速度有直接关系。汤玉玮等的研究发现,硝酸还原酶活力与作物的耐肥性呈负相关,并在综述国内外有关研究文献中指出,该酶可以做为作物育种和营养诊断的生化指标。  相似文献   

杉木硝酸还原酶活力、氮素贮藏与其生长的关系^*   总被引：6，自引：2，他引：6       下载免费PDF全文

周国璋  苏梦云 《林业科学研究》1993,6(2):141-147

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Effects of strip wise tillage in combination with liming on chemical and physical properties of acidic spruce forest soils after clear cutting     

V. Geissen  R. -Y. Kim  A. Schning  St. Schütte  G. W. Brümmer 《Forest Ecology and Management》2003,180(1-3):75-83

We investigated the initial effects of strip wise soil loosening (0–35 cm depth) on soil chemical and physical parameters by using a deeply working rotary cultivator in combination with liming and mixing of the dolomite with the soil material of acidic forests. The investigations took place 8 months after the treatment. pH values and contents of exchangeable Ca and Mg increased significantly at the tilled depth whereas the content of exchangeable Al and easily soluble P decreased. The rate of mineralisation increased at this depth which was shown by a loss of Corg, Ntot and short-term loss of NO₃-N. The treatment led to a mobilisation of Mn at the tilled depth. However, the content of exchangeable Pb decreased due to an increased pH value. Below the tillage depth of 35 cm only partly significant changes of exchangeable Mn and NO₃-N were found. The total porosity and bulk density at 10–15 and 40–45 cm depths were not significantly different from those in the control plot, but the rate of infiltration increased significantly.  相似文献   

几种树种水浸液对杉木叶片硝酸还原酶的效应   总被引：1，自引：0，他引：1  

曾火荣  曹子林 《福建林业科技》2003,30(2):42-44

采用完全随机区组设计,用马尾松(Pinusmassoniana)、木荷(Schimasuperba)、赤楠(Syzygiubuxifolium)、杉木(Cunninghamialanceolata)的枝叶1∶6浓度水浸液和清水共5个不同处理浇灌盆栽的杉木幼苗,2a后对其叶片的硝酸还原酶活性进行测定。结果表明,经过几种杉木伴生种水浸液处理2a后杉木的硝酸还原酶活性比杉木水浸液及清水处理的有较大程度的提高,其中经过马尾松水浸液处理的杉木叶片的硝酸还原酶活性最高,而经杉木枝叶水浸液处理的硝酸还原酶活性最低。  相似文献   

Nitrate reductase activity and its diurnal variation rhythm for Camptotheca acuminata seedlings     

SUNShi-qin YANXiu-feng 《林业研究》2004,15(3):167-170

Nitrate reductase activity (NRA) in different plant organs and leaves in different positions of Camptotheca acuminata seedlings was determined by an In vivo assay, the diurnal variation rhythm of NRA in leaves of different positions was observed,and the correlations between leaf NRA, leaf area and lamina mass per unit area (LMA) were also examined. The results showed that NRA in the leaf was significantly highest, compared with that in other organs such as roots, stems and leaves. In this experiment, the 10 leaves were selected from the apex to the base of the seedlings in order. The different NRA occurred obviously in leaves of different positions of C. acuminata seedlings from the apex to the base, and NRA was higher in the 4th-6th leaves.The diurnal change rhythm of leaf NRA showed a one peak curve, and maximum NRA value appeared at about midday (at 12:30 or so). No obvious correlations between NRA and leaf area or lamina mass per unit area were observed. This study offered scientific foundation for the further research on nitrogen metabolism of C. acuminata.  相似文献   

水分胁迫对三种杏树品种的硝酸还原酶的影响     

刘基伟 《绿色科技》2014,(10)

试验研究了不同水分条件下对香白杏、龙王帽杏和金太阳杏杏树体内硝酸还原酶的影响,并就影响进行了讨论。结果表明:水分对硝酸还原酶影响显著,但是极低或者过量水量对其活性有明显抑制作用,除了金太阳杏60%田间持水量外,其他品种40%和60%田间持水量都能增加硝酸还原酶活性。通过水分和活性的相关性分析表明:硝酸还原酶与活性有明显的线性相关性,证明硝酸还原酶可以作为这3种杏树抗旱生化指标。  相似文献   

Starvation Induced Alterations in Hepatic Lysine Metabolism in Different Families of Rainbow Trout (<Emphasis Type="Italic">Oncorhynchus  mykiss</Emphasis>)     

Angela?D.?Higgins  Jeffrey?T.?Silverstein  Juanita?Engles  Matthew?E.?Wilson  Caird?E.?RexroadIII  Kenneth?P.?BlemingsEmail author 《Fish physiology and biochemistry》2005,31(1):33-44

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Glutathione and its Related Enzymes in the Nile Fish   总被引：2，自引：0，他引：2  

Ragaa R. Hamed  Tahany M. Maharem  Rasha A. M. Guinidi 《Fish physiology and biochemistry》2004,30(3-4):189-199

Glutathione (GSH) and related enzymes, glutathione transferase (GST), glutathione peroxidase (GPx) and glutathione reductase (GR) form an important phase 2 biotransformation enzymes system. The objective of this study was to compare this enzymes system in three fish species from the river Nile, Oreochromis niloticus, Claris lazera and Cyprinus carpio in order to establish the main differences and to purify and characterize GST from the liver of O. niloticus.The level of GSH and the activity of GST, GPx and GR in the liver, kidney and gills of the three fish species were examined. A simple reproducible procedure for the purification of GST from the liver of O. niloticus to homogeneity, which includes chromatography on DEAE- cellulose followed by affinity chromatography on GSH-sepharose was established. The molecular mass was found to be 25,460 Da by SDS-PAGE. The Michaelis-Meneten constants (K_m) of the enzyme for GSH and CDNB were 0.35 mM and 0.42 mM, respectively. The affinity purified enzyme exhibited maximum pH at pH 8.0 and increasing pH above 8.0 did not affect the observed maximum. The purified enzyme acts readily on CDNB, less readily on some standard transferase substrates (1,2-dichloro-4-nitrobenzene and p-nitrophenethyl bromide) and not at all on others (bromosulphophthalein and p-nitrobenzyl chloride). Bromosulfophthalein, cibacron blue and hematin inhibited CDNB-conjugating activity of the purified enzyme with IC₅₀ 0.079, 3.98 and 0.126 μM, respectively.  相似文献   

A Comparison of Three Different Hydroponic Sub-systems (gravel bed, floating and nutrient film technique) in an Aquaponic Test System   总被引：1，自引：1，他引：1  

Wilson A. Lennard  Brian V. Leonard 《Aquaculture International》2006,14(6):539-550

Murray Cod, Maccullochella peelii peelii (Mitchell), and Green Oak lettuce, Lactuca sativa, were used to test for differences between three hydroponic subsystems, Gravel Bed, Floating Raft and Nutrient Film Technique (NFT), in a freshwater Aquaponic test system, where plant nutrients were supplied from fish wastes while plants stripped nutrients from the waste water before it was returned to the fish. The Murray Cod had FCR's and biomass gains that were statistically identical in all systems. Lettuce yields were good, and in terms of biomass gain and yield, followed the relationship Gravel bed > Floating > NFT, with significant differences seen between all treatments. The NFT treatment was significantly less efficient than the other two treatments in terms of nitrate removal (20% less efficient), whilst no significant difference was seen between any test treatments in terms of phosphate removal. In terms of dissolved oxygen, water replacement and conductivity, no significant differences were observed between any test treatments. Overall, results suggest that NFT hydroponic sub-systems are less efficient at both removing nutrients from fish culture water and producing plant biomass or yield than Gravel bed or Floating hydroponic sub-systems in an Aquaponic context. Aquaponic system designers need to take these differences into account when designing hydroponic components within aquaponic systems.  相似文献