Reduction in cadmium accumulation in japonica rice grains by CRISPR/Cas9-mediated editing of OsNRAMP5     

Chang-hong YANG  Yang ZHANG  Chao-feng HUANG 《农业科学学报》2019,18(3):688-697

Cadmium (Cd) intake is harmful to human health and Cd contamination in rice grains represents a severe threat to those consuming rice as a staple food. Knockout of Cd transporters is a promising strategy to reduce Cd accumulation in rice grains. OsNRAMP5 is the major transporter for Cd and manganese (Mn) uptake in rice. Nevertheless, it is uncertain whether knockout of OsNRAMP5 is applicable to produce low Cd rice without affecting plant growth and grain yield. In this study, we adopted CRISPR/Cas9-based gene editing technology to knock out OsNRAMP5 in two japonica varieties. We generated three independent transgene-free osnramp5 mutants and investigated the effect of osnramp5 mutations on Cd accumulation and plant growth. Hydroponic experiments showed that plant growth and chlorophyll content were significantly reduced in osnramp5 mutants at low Mn conditions, and this defective growth in the mutants could be fully rescued by supply of high levels of Mn. Cd and Mn accumulation in both roots and shoots was markedly reduced in the mutants compared to that in wild-type plants. In paddy field experiments, although Cd in flag leaves and grains was greatly reduced in osnramp5 mutants, some agronomic traits including plant height, seed setting rate, and grain number per panicle were affected in the mutants, which ultimately caused a mild reduction in grain yield. The reduced plant growth in the mutants can be attributed to a marked decrease in Mn accumulation. Our results reveal that the manipulation of OsNRAMP5 should be treated with caution: When assessing the applicability of osnramp5 mutants, soil pH and soil water content in paddy fields need to be taken into consideration, since they might affect the levels of available Mn in the soil and consequently determine the effect of the mutation on grain yield.  相似文献   

雾霾污染对旅游产业发展的时空动态影响及其规制研究——以长株潭城市群为例     

王兆峰  周颖  叶茂 《中南林业科技大学学报(自然科学版)》2020,(2):156-165

【目的】随着美丽中国建设和三大攻坚战等战略的推进,旅游生态环境成为旅游科学研究的重要问题,研究雾霾污染对旅游产业发展的动态影响及其环境规制作用,促进旅游节能减排。【方法】本文以两型试验区长株潭城市群为例,构建关于旅游产业评价的集成指标体系,采用重心模型和回归模型计量方法,探究旅游产业和雾霾污染发展轨迹,考察旅游产业发展与雾霾污染之间空间动态关联性,分析雾霾对旅游产业发展影响程度,检验雾霾污染与其他自变量交互作用下旅游产业发展的主导要素,最后以环境规制为门槛变量进一步讨论雾霾污染对旅游产业发展的影响,【结果】1)旅游产业重心偏移方向由长沙向衡阳方向移动,雾霾重心偏移方向在常德和株洲两地之间往返,无显著移动规律,旅游产业发展与雾霾污染之间存在一定的空间动态关联性,且两者之间存在反向抑制作用。2)雾霾污染对旅游产业发展存在影响,就研究区域而言,增加1%的雾霾污染会减少3.3%的旅游产业发展,且影响旅游产业发展的主要因素是旅游人数和环境规制。3)以环境规制为门槛变量,发现雾霾污染随着环境规制类型的不同而对旅游产业发展产生不同影响,无环境规制下,雾霾污染对旅游产业负面影响指数为0.8%,对旅游产业发展影响显著;宽松型环境规制下,雾霾污染对旅游产业负面影响为0.2%,影响程度略小;严密型环境规制下,雾霾污染对旅游产业负面影响为0.1%,影响性最小。【结论】雾霾污染对旅游产业发展存在负面影响,但政府环境政策可有效缓解雾霾污染,因此在环境规制下,雾霾污染对旅游产业发展的负面影响减弱。  相似文献   

硒肥与钝化材料组配对土壤Cd钝化及稻米Cd消减效果   总被引：1，自引：0，他引：1  

柴冠群  刘桂华  罗沐欣键  秦松  范成五 《中国农学通报》2021,37(32):102-107

为了探讨不同硒肥施用方式联合钝化材料对土壤镉钝化和稻米镉消减的效果,采用盆栽试验的方式,选用亚硒酸钠作为硒肥,钙镁磷肥和硅藻土作为钝化材料,设置基施硒肥+钙镁磷肥+硅藻土和叶面喷施硒肥+钙镁磷肥+硅藻土2种方式,研究其不同用量对镉污染酸性稻田土壤修复与安全利用的影响。结果表明：随着施用量的增加,稻米产量增加,基施硒肥产量略高于叶面喷施硒肥,产量差为 2.115 g/pot,与对照(CK)相比,基施0.28%钙镁磷肥+0.12%硅藻土+0.004‰硒(T3)能够提高1.68倍的稻米产量;随着施用量的增加,pH升高,有效Cd降低,有机质与CEC变化不大;基施硒肥与叶面喷施硒肥处理对土壤pH、有机质与CEC差异不显著,但基施硒肥处理有效Cd含量略低于叶面喷施硒肥处理,T3对土壤Cd的钝化效果最佳;随着基施硒肥用量的增加,稻米Cd含量降低,随着叶面喷施硒肥用量的增加,稻米Cd含量先降低后升高,基施硒肥处理对稻米Cd的消减程度强于叶面喷施硒肥处理,相差 0.021 mg/kg,与对照(CK)相比,T3处理稻米Cd降低0.063 mg/kg。可见,硒对调控稻米镉累积具有重要作用,且基施硒肥强于叶面喷施。综上所述,基施0.28%钙镁磷肥+0.12%硅藻土+0.004‰硒对土壤Cd钝化与稻米Cd消减的效果最佳,值得在镉污染稻田推广应用。  相似文献   

Use of the nitrate‐specific ion electrode for the determination of nitrate nitrogen in surface and ground water     

《Communications in Soil Science and Plant Analysis》2012,43(6):415-420

Abstract

The usefulness of the nitrate‐specific ion electrode (NE) for the determination of nitrate nitrogen (NO3‐N) in surface and ground water was determined. The NE method had several advantages that made it superior to the phenoldisulfonic acid (PDS) method in our environmental quality studies. Samples could be analyzed quickly and with a minimum of sample preparation. Sample coloration and soluble salts did not interfere with the performance of the electrode. Range of detection without dilution was much wider than by the PDS method. Also, the values obtained by the NE method, although slightly greater than those obtained by the PDS method, were satisfactory.  相似文献   

Environmental Change and the Sustainability of European Forests     

《Journal of Sustainable Forestry》2013,32(2-3):165-187

Summary

It is some years now since forest decline was a major public concern in Europe and was one of the principal environmental issues around which international research programs were focused. A number of internationally coordinated activities were initiated in the 1980s and 1990s and have continued until now; these contributed significantly to our current understanding of forestry and to the way in which forestry policies have developed. In short, the concept of sustainable development has had an increasing influence and is now of immeasurable value in forest policy, with sustainable forest management well established as its guiding principle. This sequence of events is examined here. The extent to which understanding has advanced is remarkable; much has changed. Arguably this period gave the first indication of the extent to which forests and ecosystems globally are threatened by environmental change. On the basis of the last 20 years, it is tempting to conclude that we now have an effective institutional framework and have made excellent progress. However, some of the recorded ecosystem responses seem anomalous; there are surprises in system responses, e.g., the linkage between sulphur and nitrogen depositions and forest growth. Even more importantly some specific pollutant problems remain and will intensify, and climate change has become an environmental issue of overwhelming importance.  相似文献   

Fas对镉激活PC12细胞线粒体凋亡通路的影响     

闻双全  陈洁  王莉  邹辉  顾建红  刘学忠  卞建春  刘宗平  袁燕 《畜牧兽医学报》2021,52(8):2326-2333

旨在探究死亡受体Fas在镉致大鼠肾上腺嗜铬细胞瘤细胞（PC12）凋亡中的作用及其对线粒体通路的调控机制,用10 μmol·L^-1镉处理Fas基因沉默的PC12细胞株12 h,通过Western blot检测BH3相互作用域死亡激动剂（BID）、半胱氨酸蛋白酶-9（caspase-9）、半胱氨酸蛋白酶-3（caspase-3）、多聚二磷酸腺苷核糖聚合酶（PARP）的活化情况,Bcl-2相关X蛋白（Bax）、B细胞淋巴瘤/白血病-2基因（Bcl-2）、凋亡诱导因子（AIF）、核酸内切酶G（Endo G）的表达情况,以及细胞色素C（Cyt C）在细胞内的分布情况,免疫荧光染色检测AIF核转位。结果显示,镉极显著上调tBID/BID比值和Bax/Bcl-2比值,诱导Cyt C从线粒体释放到细胞质,激活caspase-9、caspase-3和PARP,增加AIF和Endo G蛋白表达水平（P<0.01）,并诱导AIF核转位;沉默Fas极显著抑制镉引起的tBID/BID比值和Bax/Bcl-2比值升高,Cyt C从线粒体释放到胞浆,caspase-3、PARP蛋白活化和AIF、Endo G蛋白表达水平极显著升高（P<0.01）,显著抑制镉激活的caspase-9（P<0.05）,并抑制AIF核转位。综上表明,Fas通过调控线粒体通路参与镉致PC12细胞凋亡。  相似文献   

中兽医药技术是猪只康养最佳供给体系     

张鑫義  王孝武 《中兽医医药杂志》2021,(1):78-81

在我国养猪业面临产品安全和环境污染两大瓶颈的情势下,如何使所生产的猪肉食用卫生,所排粪污环境安全,纯天然、无污染、广效低毒、绿色环保的中兽医药学为破解这两大瓶颈提供了技术支撑。充分发挥我国传统的中兽医药技术的特色和优势,构建以中兽医药为主体的猪只康养技术供给体系,旨在集生产管理"适其天性"、所喂药料"无污染"、治疗药物"纯天然"、预防措施治"未病"、治疗方法"和谐调节"和四季保健"药饲同源"于一体的技术供给体系,从源头上破解这两大难题,从而实现猪只康养,环境安全,猪肉安康,凸显出中兽医药是猪只康养的最佳供给体系。  相似文献   

浅析发酵乳霉菌零星污染来源及控制方法     

马维  宋波  陈文静  柴雪娇  杨琴芬 《中国乳业》2021,(7):87-90

霉菌在自然环境中普遍存在,而且以孢子形态随空气、水等媒介进行扩散。对食品原辅料及生产环境均可造成污染,是造成发酵乳变质的主要原因。发酵乳作为保质期较短且需冷链储运、销售的产品,零星的霉菌污染不会引起食品安全问题,但由于部分销售终端及三四线市场冷链运输缺失,造成霉菌生长,发酵乳呈现明显霉斑,导致消费者体验差,给企业品牌造成极坏影响,也影响了食品安全。本文概述了发酵乳中来自原辅料、环境、水中霉菌零星污染来源、验证方法以及相应控制措施,以期为发酵乳中霉菌控制提供参考。  相似文献   

资料缺乏地区水功能区纳污能力计算——以都匀市清水江为例     

李红军  刘锐  张建元  魏文杰  张新华 《节水灌溉》2015,(5)

水功能区限制纳污红线的确立是实行最严格水资源管理制度的重要组成部分。然而在河流纳污能力量化的过程中往往因为流量、流速等数据的缺失或不足导致计算结果不够准确。SWAT分布式水文模型可以通过DEM生成河网并结合气象等数据计算出河道的流量和流速,模型的这一优势能够很好地解决目标河道水文资料缺乏的问题。以黔南州都匀市清水江为例,通过构建SWAT模型和一维水质模型计算出该河流各个水功能区的纳污能力,研究结果可为该河流的水污染总量控制提供数据支撑。  相似文献   

Protective Effect of Sulforaphane on Reproductive Function of Male Mice with Cadmium Poisoning     

GAO Feng  HE Jian-bin  ZHANG Yi  LONG Miao  LI Lin  LI Yan-peng  HAN Yang  WANG Xue  JIANG Li-ying  YANG Shu-hua 《中国畜牧兽医》2016,43(2):471-476

The study was aimed to explore the protective effect of sulforaphane (SFN) on the reproductive function of male mice with cadmium poisoning.40 healthy clean grade male Kunming mice were randomly divided into four groups:control group (H₂O),cadmium chloride group (2.3 mg/kg CdCl₂),sulforaphane group (10 mg/kg SFN),sulforaphane + cadmium chloride group (10 mg/kg SFN+2.3 mg/kg CdCl₂),and continuous administration for 10 d,all mice were executed by dislocated cervical vertebra at 2 d after the last administration,and then the pathologic changes of testicular tissues,organ coefficient of testicle and epididymis,sperm quality and concentration of testosterone were tested.Additionally,the contents of GSH and MDA,and the activities of T-SOD in testis were also detected at the same time. Compared with the control group,pathology damages were observed in cadmium chloride group,organ coefficient of testis and epididymis,sperm quality and levels of testosterone extremely significantly decreased (P<0.01),the activities of T-SOD and GSH content were extremely significantly decreased (P<0.01),and the concentration of MDA was extremely significantly enhanced (P<0.01).Compared with the control group,the activity of T-SOD and concentration of GSH in sulforaphane group were significantly increased (P<0.05),and the concentration of MDA was not significant different between the control group and sulforaphane group (P>0.05).While compared with the cadmium chloride group,the sperm motility rate and sperm total count in sulforaphane and cadmium chloride group were extremely significantly increased (P<0.01),the organ coefficient of testicle and epididymis was increased significantly (P<0.05),the concentration of GSH and activity of T-SOD in testicular tissue were extremely significantly increased (P<0.01),and the concentration of MDA was extremely significantly decreased (P<0.01).The results indicated that sulforaphane had the protection effect on reproduction function of male mice with cadmium poisoning.  相似文献