Assessment of postoperative adjuvant treatment using toceranib phosphate against adenocarcinoma in dogs     

Hiroki Yamazaki  Toshiyuki Tanaka  Keiichiro Mie  Hidetaka Nishida  Naoki Miura  Hideo Akiyoshi 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(3):1272-1281

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支持电解质浓度对磷酸根在可变电荷土壤表面吸附和解吸的影响   总被引：2，自引：0，他引：2  

程程  姜军  徐仁扣 《土壤学报》2011,48(3):557-562

研究了离子强度对2种可变电荷土壤中磷酸根吸附和解吸的影响。结果表明,当pH分别大于3.7和4.0时,红壤和砖红壤对磷酸根的吸附量随离子强度的增加而增加;当pH分别小于3.7和4.0时,红壤和砖红壤对磷酸根的吸附量随离子强度呈相反的变化趋势。电解质主要通过改变离子专性吸附面上的电位来影响磷酸根的吸附。Zeta电位的测定结果表明,当pH大于土壤胶体的等电点(IEP)时,吸附面上电位为负值,且随离子强度增加数值减小,对磷酸根的排斥力减小,土壤表面对磷酸根的吸附量增加;当pH小于IEP时,吸附面上的电位为正值,它随离子强度增加而减小,不利于磷酸根的吸附。解吸实验的结果表明,吸附于可变电荷土壤表面的磷酸根在去离子水中的解吸量高于0.1 mol L-1NaNO3体系中的解吸量。这同样由于电解质浓度对土壤表面吸附面上的电位的影响所致。  相似文献   

转phyA基因棉花纯合株系筛选及其对土壤有机磷的利用能力   总被引：2，自引：1，他引：1  

黄惠  王省芬  郭彩菊  刘建凤  王敬敬  李志坤  吴立强  张桂寅  马峙英 《棉花学报》2011,23(3):219-223

 为了获得转植酸酶基因（phyA）棉花高代纯合株系并验证其利用土壤中有机态磷的能力,对实验室获得的转phyA基因T₄代材料进行了PCR筛选,在水培条件下研究了纯合株系植株根际分泌的植酸酶活性,并在田间进行了植株磷含量和棉花产量性状分析。结果表明,在PCR筛选的4个株系中,有2个株系为纯合株系,其后代均为PCR阳性植株。在以植酸盐为唯一磷源条件下,高表达phyA的转基因株系的植酸酶活性较野生型增加了1.5倍,不同生育时期转基因株系植株叶片磷含量中L6株系增加最多,苗期、现蕾期、花铃期和吐絮期分别增加了4.5%,5.95%,5.45%和8.73%。  相似文献   

Mycoplasma synoviae cell invasion: Elucidation of the Mycoplasma pathogenesis in chicken     

Marcos R. BuimMelissa Buzinhani  Maurício YamagutiRosângela C. Oliveira  Elena MettifogoPriscila M. Ueno  Jorge TimenetskyGláucia M.M. Santelli  Antonio J. Piantino Ferreira 《Comparative immunology, microbiology and infectious diseases》2011,34(1):41-47

Fluorochrome-labelled cells of two field isolates and Mycoplasma synoviae (Ms) were inoculated onto monolayer cultures of fluorochrome-labelled HEp-2 cells and monitored by confocal laser scanning microscopy (CLSM). Ms was detected initially adhered to and subsequently inside the host cells. Between 24 and 48 h of infection, Ms was detected in the perinuclear region, and after 72 h of infection was confirmed by gentamicin invasion assay. High and low passage Ms strains showed no differences in adherence or invasion. The morphology and the actin filaments of the infected HEp-2 cells were preserved throughout the study period. The observed invasion by Ms is consistent with the biology of Mollicutes, and could explain the difficulties in recovering field isolates of the mycoplasma and in controlling the infection in birds even after long-term antibiotic treatment.  相似文献   

Polyphenolic compounds and antioxidant properties in mango fruits     

Xiaowei Ma  Hongxia WuLiqin Liu  Quansheng YaoSongbiao Wang  Rulin ZhanShanshan Xing  Yigang Zhou 《Scientia Horticulturae》2011

Fruit of eight mango genotypes were evaluated for antioxidant potential by several biochemical assays (DPPH, ABTS, ORAC, FRAP, SASR and MCC) and tested for their polyphenol composition and vitamin C contents. The significance analysis demonstrated that the antioxidant capacity of Tainong 1 fruits were significantly higher than that of other genotypes, which was about 2.1–6.3-fold higher than Guifei assayed in ABTS, DPPH, ORAC and FRAP methods. The total polyphenols and flavonoids contents showed a great variety amongst mango genotypes and highly correlation with the total antioxidant capacity. It is concluded that significant genotypic difference exists in the total antioxidant capacity of mango fruits. Both total polyphenols and flavonoids are major contributors to the total antioxidant capacity in mango fruit.  相似文献   

Simple method for isolation of glyceraldehyde 3‐phosphate dehydrogenase and the improvement of myofibril gel properties     

Yuji MIYAGUCHI  Taro SAKAMOTO  Shun SASAKI  Koji NAKADE  Manabu TANABE  Satoko ICHINOSEKI  Masahiro NUMATA  Kiichi KOSAI 《Animal Science Journal》2011,82(1):136-143

Porcine glycoliytic enzyme, glyceraldehyde 3‐phosphate dehydrogenase (G3PD) was prepared effectively by a combination of ethylene diamine tetra‐acetate (EDTA) pretreatment and affinity purification. After salting out of porcine sarcoplasmic proteins (SP) with ammonium sulfate at 75% saturation, the obtained supernatant (SP‐f3) was treated with EDTA, leaving G3PD in the supernatant (G3PD‐E) and most other SPs in the precipitate. At that time, the separation of G3PD‐E required more than 20 mmol/L EDTA. G3PD‐E was then subjected to affinity purification by batchwise method using blue‐sepharose CL‐6B, and purified G3PD (G3PD‐AP) was obtained using 2 mol/L potassium chloride (KCl) as an eluent. Texture analysis showed that the hardness, adhesiveness and gumminess of the myofibril gel at 0.2‐mol/L NaCl increased with the addition of G3PD‐AP. Scanning electron microscopy revealed that the G3PD‐AP reinforced the gel network of the myofibril. However, scanning electron micrograph analysis showed that the network‐structure of the gel by the addition of G3PD‐AP developed in a different manner from that by adding 0.6 mol/L NaCl. These results showed that glycolytic enzyme, G3PD, contributes to the improvement of the rheological properties of meat products.  相似文献   

磷酸化针铁矿表面次级吸附态锌的化学分组   总被引：8，自引：0，他引：8  

介晓磊  刘凡 《华中农业大学学报》1997,16(5):340-344

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磷钾细菌解磷解钾能力的研究   总被引：4，自引：0，他引：4  

赵晨曦  刘前刚  张志元 《湖南农业大学学报(自然科学版)》2004,30(6):519-521

为了筛选解磷解钾能力强的磷钾细菌新菌株用于有机菌肥的研制，分别以磷矿粉和钾长石为底物进行磷钾细菌的摇瓶发酵试验，并测定发酵液中有效磷钾的含量，选择解磷解钾能力强的磷钾细菌进行米高粱盆栽试验，当植株生长至拔节时切取地上部分测定其生物产量和植株内磷钾元素含量．结果表明：大部分喷施菌液的植株磷钾元素含量及其生物产量均明显高于对照，其中混合菌株发酵液的处理生物产量最高，由此确定S-2，S-6，S-12和ACCC10010为新菌肥生产用菌株组合.  相似文献   

施磷肥对土壤磷素形态转化和有效性的影响   总被引：3，自引：1，他引：3  

甄清香  邵煜庭 《甘肃农业大学学报》1994,29(4):392-395

通过室内土壤培育和盆栽试验，研究了施磷肥和培育时间对灌漠土、黑垆土和褐土无机磷形态转化及有效性的影响。结果表明施入石灰性土壤的磷肥，除作物吸收利用外，主要以Ｃａ＿２—Ｐ、Ｃａ＿８—Ｐ、Ａｌ—Ｐ的形态积累；随着培育时间的推移，Ｃａ＿２—Ｐ、Ｃａ＿８—Ｐ又明显降低，并经由Ａｌ—Ｐ、Ｆｅ—Ｐ逐渐向Ｃａ＿１０—Ｐ转化。经生物试验和相关分，Ｃａ＿２—Ｐ、Ａｌ—Ｐ和Ｃａ＿８—Ｐ均是灌漠土、黑垆土和褐土的有效磷源，以Ｃａ＿２—Ｐ的贡献最大。  相似文献   

不同添加材料对化肥中磷素释放和扩散的影响   总被引：1，自引：0，他引：1  

杨莹  杨雪芹  关文玲 《西北农林科技大学学报(自然科学版)》2005,33(6):84-86,92

采用不同的添加材料与化学磷肥混合,通过室内扩散试验,研究不同添加材料对化学肥料中磷素释放和扩散的影响。结果发现,选用的5种材料(沸石、胡敏酸、聚丙烯酰胺、柠檬酸、羧甲基纤维素)均能不同程度地提高肥料磷在土壤中的有效性。与对照相比,柠檬酸在培养初期(1周)能够促进化学磷肥的溶解和扩散,可使施肥点和外围的速效磷含量升高约2.7倍;胡敏酸、PAM在培养4周后能提高施肥点的速效磷,而且使最外层(7～9cm)的速效磷也有较明显的增加,分别较对照提高75%和68%。  相似文献