亚麻纤维素合酶LuCesA8基因克隆与表达分析     

于莹  郭文栋  赵丽娟  吴建忠  程莉莉  赵东升  胡莹莹  吴广文  关凤芝 《东北农业大学学报》2016,(8)

以亚麻快速生长期茎部组织总RNA为模板,通过反转录PCR克隆纤维素合酶基因Lu Ce s A8(基因ID:Lus10029245)全长开放读码框序列。序列分析结果表明,开放读码框全长2 967 bp,共编码988个氨基酸。通过多氨基酸序列比对发现,该蛋白与麻风树Ces A8蛋白亲缘关系最近,相似度达87%,与胡杨、可可树、雷蒙德氏棉及芝麻Ces A8蛋白氨基酸序列相似性分别为86%、85%、85%、81%。荧光定量PCR结果表明,该基因在亚麻不同发育阶段表达水平存在显著差异,快速生长期表达量最高,花期和绿熟期次之,苗期最低。研究为揭示Lu Ce s A8基因在次生细胞壁纤维素合成中的作用奠定基础。  相似文献   

全喂入式胡麻脱粒机的设计与试验   总被引：2，自引：0，他引：2  

史瑞杰  戴飞  赵武云  张仕林  张锋伟  刘小龙 《中国农业大学学报》2019,24(8):120-132

针对胡麻籽粒小、茎秆易缠绕、喂入流动性差等特点,采用垂直排列D型纹杆闭式脱粒滚筒和菱形棱角型栅栏式凹板组和的方式,设计一种全喂入式胡麻脱粒机。以陇亚14号胡麻为试验材料,对其茎秆进行生物力学特性试验,运用ANSYS Workbench和Fluent对脱粒机机架和风选系统分别做模态及流场模拟分析,并进行脱粒试验。结果表明:当陇亚14号胡麻中部茎杆含水率为9.43%时,抗拉强度为179.6 MPa,抗弯强度1.79 MPa,剪切强度0.19 MPa;机架前8阶固有频率范围为46.19～257.20 Hz,振幅范围7.53～115.63 mm,在第6阶频率190.46 Hz处出现最大振幅为115.63 mm,在第5阶固有频率190.01 Hz处出现第2大振幅114.99 mm;当脱粒滚筒主轴转速为1 500 r/min时,垂直悬浮筒进口风速达到了6.3～10 m/s,离心风扇径向边缘风速可达50 m/s,垂直悬浮筒进口到离心风扇之间的压强在-500～-1 000 Pa,离心分离筒内壁面压强4 000 Pa,排杂口压强4 799.27 Pa;脱粒时作业机脱净率为98.86%,含杂率2.15%,破碎率0%、夹带损失率0.8%、清选损失率0.27%、飞溅损失率1.8%、未脱净损失率0.09%,总损失率2.97%。该机整体设计符合国家标准要求,满足胡麻脱粒作业需求。  相似文献   

通径分析在油纤兼用亚麻产量分析中的应用     

刘海龙  王世发  周玉萍  牛海龙  刘淑莲  潘亚丽  孙晓平  徐迟 《安徽农业科学》2012,40(11):6416-6417,6419

首先对18个胡麻(Linum usitatissimum Linn.)品系的5个产量构成性状及产量进行相关分析,指出单株粒数和分枝长与产量之间的相关性达显著水平,并且各性状之间还存在着相互作用、相互制约和相互促进的关系。因此对吉林省胡麻产量及其构成性状的关系须采用通径分析方法进行分析,以估算每一个性状对产量的直接作用,以及一个性状通过另一个性状对产量的间接作用。通径分析结果表明,在吉林省提高胡麻产量,可主要通过提高单株粒数和增加分枝数来实现;返青将致使胡麻减产。  相似文献   

Two in vitro assays to evaluate resistance in Linum usitatissimum to Fusarium wilt disease     

G.M.L.W. Kroes  E. Sommers  W. Lange 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(6):561-568

Two types of in vitro seedling tests were developed to evaluate resistance in flax (Linum usitatissimum) against Fusarium oxysporum f.sp. lini. In the first test a solid medium was used. The second test was based on a liquid medium. Disease severity was assessed after three weeks, using the observed reduction of plant length as a scale. Both methods proved to be useful for screening for resistance, for evaluating race specificity of resistance and for studying symptomatology. The solid medium method proved to be the more accurate for the screening, but the liquid medium method was much less time- and labour-consuming. The results of the tests were significantly correlated with observations under field conditions.  相似文献   

除草剂残留对亚麻的影响及预防措施     

夏尊民  宋淑敏 《中国麻业》2007,(4)

长残效除草剂大面积施用及超量施用,对后茬作物亚麻造成严重危害,已成为影响我国北方亚麻主产区产业发展的重要因素。本文阐述了亚麻受药害原因及症状表现,列举了对后茬亚麻有影响的除草剂种类并提出了预防措施。  相似文献   

云南发展亚麻产业的思路及对策   总被引：3，自引：1，他引：2  

刘其宁  赵振玲  杜刚  吴学英 《中国麻业科学》2007,29(5):295-297,301

本文从分析云南亚麻产业发展的可能性和必要性及产业发展现状和存在问题入手，提出产业发展思路及对策，为云南省纤维亚麻的发展提供依据。  相似文献   

亚麻快速生物脱胶技术研究Ⅱ．亚麻快速生物脱胶过程中发酵液成分变化规律   总被引：6，自引：2，他引：4  

《中国麻业》2004,26(1):31-34

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Response of grain yield and related agronomic traits of oilseed flax to diversified cropping rotations北大核心CSCD     

王海娣  张勇  高玉红  吴兵  剡斌  崔政军  王一帆  张雪 《草业学报》2022,31(12):52-65

This research aimed to identify a suitable planting pattern for oilseed flax production in a dry-farming region. A long-term field experiment was started in 2012 with a 4-year crop rotation cycle,designed to measure the effects on flax crop performance of previous crop,and various patterns of flax planting interval within the four-year rotation,compared with continuous flax cropping. Crop parameters measured included plant height,stem diameter,dry matter accumulation and distribution,and the experiment included six different crop rotation patterns：Flax→flax →flax→flax［（F）FFF］;Flax→wheat→potato→flax［（F）WPF］;Flax→potato→flax→wheat［（F）PFW］;Flax→ flax→wheat→potato［（F）FWP］;Flax→wheat→flax→potato［（F）WFP］and flax→wheat→potato→wheat［（F）WPW］. Results for the ninth year showed significantly increased grain yield（29. 89%-109. 57%）in crop rotation treatments compared with continuous cropping of oilseed flax. The ranking of the six tested rotations for yield was：（F）WPW>（F）FWP>（F）WFP>（F）PFW>（F）WPF>（F）FFF. The grain yield of oilseed flax was significantly affected by previous crop,frequency and years interval of flax cropping and number of years of continuous flax cropping. Yield was increased by 54. 45%-59. 29% under wheat stubble and potato stubble compared with oilseed flax stubble,and increased by 30. 66% and 109. 57%,respectively,under 50% and 25% frequencies,compared with 100% frequency. The grain yield of oilseed flax under two-year continuous cropping was higher by 29. 89% than four-year continuous cropping,and increased with increase in years interval between flax crops. Correlation analysis identified a significantly positive correlation between oilseed flax grain yield and effective capsule number,branch number and 1000-seed weight. The effective capsule number,branch number and 1000-seed weight of oilseed flax under rotation treatment were increased by 35. 88%-108. 91%,15. 47%-46. 19% and 14. 61%-16. 34%,respectively（P<0. 05）,compared with continuous cropping. In addition,the high grain yield of oilseed flax was accompanied by an increase in plant height,stem diameter and dry matter accumulation and these increases were,respectively,5. 11%-42. 24%,2. 77%-39. 92% and 31. 25%-117. 89% under the rotation regimes,compared with continuous cropping. Reduction in the number of years of continuous cropping years,change of crop stubble,decreased flax planting frequency and increased of interval between flax crops also improved flax crop performance. In summary,crop rotation improved the vigor of oilseed flax,resulting in greater plant height and stem diameter,improved dry matter accumulation and distribution,leading to increased branch number,effective capsule number and 1000-seed weight,and increase in the crop yield of oilseed flax. The results indicated that a multiple-crop rotation pattern was an effective way to avoid the yield reduction caused by continuous cropping in oilseed flax. The rotation： Flax→wheat→potato→wheat performed best among those tested and can be recommended as an appropriate cropping rotation for oilseed flax production in the dry region of northwest China. © 2022 Editorial Office of Acta Prataculturae Sinica. All rights reserved.  相似文献   

亚麻萌发期耐盐鉴定体系优化及150份种质耐盐性综合评价     

李枭  郭栋良  李恭泽  薛敏  江海霞  叶佳丽  谢丽琼 《新疆农业科学》2022,59(6):1438-1449

【目的】 优化亚麻萌发期耐盐鉴定体系并综合评价种质资源耐盐性,为亚麻种质资源耐盐性评价及耐盐品种培育奠定基础。【方法】 选用芽长、根长和发芽率作为亚麻萌发期耐盐性鉴定的表型性状。利用13个NaCl浓度梯度胁迫处理30份亚麻种质,分析不同指标变化确定胁迫浓度。用确定的浓度对150份亚麻种质进行胁迫,分别统计相对芽长、相对根长和相对发芽率,利用隶属函数法对供试亚麻萌发期耐盐性进行综合评价,结合聚类分析划分耐盐等级。【结果】 以相对芽长或相对根长为指标时适宜的NaCl胁迫浓度为100 mmol/L,以相对发芽率为指标时适宜的NaCl胁迫浓度为220 mmol/L;150份亚麻种质分划为5个耐盐等级:高耐3份、耐盐36份、中耐67份、敏感33份、高敏11份;油用亚麻亚群中各指标均显著高于纤维亚麻和兼用亚麻。【结论】 不同耐盐指标的最适筛选浓度不同。筛选出的3份高耐亚麻种质可用于亚麻耐盐育种及后续研究。油用亚麻相比兼用和纤用亚麻更耐盐。  相似文献   

油用亚麻主要品质和农艺性状的变异分析     

伊六喜  萨如拉  范鑫  赵灿  李茹  斯钦巴特尔 《中国农业科技导报》2022,24(5):56-67

为深入了解油用亚麻主要品质和农艺性状的遗传变异,以253份油用亚麻种质为研究对象,在3个环境下（内蒙古呼和浩特市、集宁市和锡林郭勒盟太仆寺旗）对其全生育天数、株高、工艺长度、单株果数、每果粒数、单株粒重、千粒重和分枝数8个农艺性状及棕榈酸、硬脂酸、油酸、亚油酸、亚麻酸、粗脂肪含量6个品质性状进行鉴定。结果表明,8个农艺性状的变异系数为5.66%~42.65%;6个品质性状的变异系数为4.10%~30.14%。全生育天数在太仆寺旗表现最长,为112.51 d;千粒重和单株粒重在集宁地区表现最大,分别为5.94和0.55 g;果粒数和单株果数在呼和浩特地区表现最多,分别为5.65和16.90;粗脂肪和亚麻酸含量在太仆寺旗地区表现最大,分别为39.53%和53.45%;亚油酸和棕榈酸含量在集宁地区最大,分别为16.41%和5.09%;油酸和硬脂酸含量在呼和浩特地区表现最大,分别为24.03%和8.31%。聚类分析表明,253份油用亚麻种质被划分为4个类群,相同地理来源的油用亚麻种质被聚到1个类群,为油用亚麻种质资源的收集保存与繁殖提供了科学依据。  相似文献