Formation of Biogenic Amines in Delayed Salt-Cured Whole and Gutted Indian Oil Sardines (Sardinella longiceps)     

Thamizhselvan Surya  Robinson Jeya Shakila  Narayanan Asha Hema Malini  Mani Palanikumar  Venkatesan Alamelu 《Journal Of Aquatic Food Product Technology》2018,27(3):394-404

The formation of biogenic amines in Indian oil sardines (Sardinella longiceps) collected from the Tuticorin coast of South India and treated with delayed salt-curing was investigated. Sardines were wet salt-cured in whole and gutted forms and examined in fresh and after 6, 9, 12, and 15 h delayed conditions at ambient temperature (32 ± 2°C) at four stages of salt-curing process for quality parameters and formation of biogenic amines. Moisture content decreased from 76% to 11.3% in salting followed by drying. Similarly, water activity reduced from 0.96 to 0.74 in salt-cured sardines. In contrast, total volatile base nitrogen content increased from 79 to 3,590 mg kg^–1 in salt-cured sardines. Halophilic count of sardines was higher (8 log cfu g^–1) after salting and decreased in drying stages. The 15 h delayed salt-cured whole sardines had higher histamine contents (1,568.27 mg kg^–1), cadaverine (4,059.84 mg kg^–1), putrescine (1,604.95 mg kg^–1), and tyramine (862.94 mg kg^–1) on the final day of drying. Whole sardines had higher biogenic amines and halophilic bacterial counts than the gutted sardines. The whole and gutted sardines salt-cured up to a delay of 6 and 12 h, respectively, at ambient temperature yielded the acceptable quality products with respect to biogenic amines.  相似文献   

烟叶烟碱含量与腐胺N-甲基转移酶基因表达间的相关性     

张敏  顾勇  赵锦超  彭勇  张永辉  徐传涛  谢强  夏建华  张军杰  鲁黎明 《贵州农业科学》2017,45(9)

为探明烟碱合成与积累机制,采用大田试验方法,测定云烟87和红花大金元在四川省6个产区烤后烟叶的烟碱含量,检测其在旺长期、现蕾期及成熟期PMT基因的表达量,并分析PMT基因表达量与烟碱含量之间的相关性。结果表明:四川不同产区不同烤烟品种的烟碱含量差异较大,云烟87和红花大金元在古蔺烟区烟碱含量最高,分别为3.40%和4.37%;云烟87在米易烟区烟碱含量最低,为1.27%,红花大金元在会理烟区烟碱含量最低,为1.36%。经相关性分析,2个品种的烟碱含量均与成熟期PMT基因表达量呈极显著正相关,与现蕾期基因表达量呈显著(红花大金元)或极显著(云烟87)正相关。PMT基因表达量是影响烟碱含量的决定因素之一。  相似文献   

NaCl胁迫下外源腐胺和钙对草莓幼苗离子吸收的影响   总被引：1，自引：0，他引：1  

李青云  葛会波  胡淑明 《植物营养与肥料学报》2008,14(3):540-545

在150 mmol/L NaCl 胁迫条件下,以达塞莱克特草莓为试材,利用基质盆栽方法研究了外源腐胺和钙对草莓生长、叶片含水量、脯氨酸含量及Ca2+、Na+ 和 Cl- 吸收的影响。结果表明,在营养液中添加14 mmol/L 硝酸钙和叶面喷施 1×10-5 mol/L 腐胺均可提高盐胁迫草莓植株的地上部和地下部干鲜重,提高叶片含水量、脯氨酸含量,抑制 Na+ 和Cl- 的吸收及运输,提高根系和叶片的 K+/Na+ 值和 Ca2+/ Na+ 值,硝酸钙和腐胺同时处理的效果优于单独处理。缺钙条件下,喷施外源腐胺的 K+/Na+ 值和 Ca2+/ Na+ 值显著或极显著低于腐胺加钙处理,Cl- 含量显著高于腐胺加钙处理。外源腐胺对 Na+ 和 Cl- 吸收的调控作用受到 Ca2+ 的调节。  相似文献   

Water Stress-induced Injury to Reproductive Phase in Chickpea: Evaluation of Stress Sensitivity in Wild and Cultivated Species in Relation to Abscisic Acid and Polyamines     

H. Nayyar  S. Kaur  Smita    K. J. Singh  K. K. Dhir  T. Bains 《Journal of Agronomy and Crop Science》2005,191(6):450-457

Chickpea (Cicer arietinum L.) is more sensitive to water stress during its reproductive growth and consequently experiences substantial yield loss. Wild species being relatively tolerant to abiotic stresses offer a potential gene source for incorporation of drought tolerance in cultivated species. In the present study, the differential stress sensitivity of wild (Cicer reticulatum Ladiz.) and cultivated species (C. arietinum) was evaluated by subjecting the plants of both the species to water stress for 14 days during the reproductive phase and examined for stress injury, endogenous status of polyamines (PA) and abscisic acid (ABA). Comparatively, the cultivated species experienced higher stress injury (assessed as electrolyte leakage, 2,3,5‐triphenyl tetrazolium chloride reduction ability, chlorophyll loss) as well as decreased leaf water potential (Ψ_w; ?2.48 MPa) than the wild species (?1.98 MPa). The stressed plants of cultivated species lost more number of flowers (62 %) and pods (65 %) when compared with the unstressed ones while those of wild species showed 30 % and 44 % loss, respectively. The number of infertile pods was significantly more in stressed plants of cultivated species than in those of wild species. The endogenous status of PA revealed that putrescine (PUT), spermidine (SPD) and spermine (SPM) levels accumulated to a higher extent in the cultivated species relative to the wild species until the eighth day of stress, and thereafter, the wild species had significantly higher levels of PA. In contrast, ABA levels increased to a higher extent in the wild species until the eighth day of stress and declined subsequently to a greater degree than the cultivated species. The injury to reproductive growth appeared to accentuate with reduction in leaf Ψ_w that was coupled with depletion of PA in both the species, especially in the cultivated one. Exogenous application of 10 mm PUT partially alleviated the damage to reproductive growth by increasing the leaf water status and chlorophyll content while decreasing the ABA content, which resulted in improvement of retention of flowers and pods, particularly in the cultivated species.  相似文献   

外源腐胺提高荔枝子房中抗氧化酶活性和延长柱头容受性     

刘顺枝  李建国  王泽槐  罗诗  尹金华 《华南农业大学学报》2003,24(3):16-19

以'糯米糍'荔枝(LitchichinensisSonn.cv.Nuomici)为试材,研究了开花前2d喷施30mg·L-1腐胺(Put)1次对子房的抗氧化酶活性和柱头容受性的影响.结果表明:在雌花开放前2d喷施Put后,不同授粉时期的处理均不同程度地提高了子房中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性;在雌花开放的第4d授粉,处理的坐果率为3 64%,而对照则不能坐果.据此认为喷施Put提高坐果的重要原因之一可能与Put提高了抗氧化酶活性,从而延缓了雌蕊衰老和延长了柱头容受花粉能力有关.  相似文献   

Experimental production of gastric dilation and its association with osmoregulatory stress and biogenic amines in chinook salmon, Oncorhynchus tshawytscha (Walbaum)     

Lumsden JS  Marshall S  Gillard M  Wybourne B  Minamikawa M 《Journal of fish diseases》2003,26(8):469-476

Chinook salmon smolt in fresh water fed a commercial diet known to produce minimal gastric dilation and air sacculitis (GDAS) were randomly assigned to four experimental tanks with flow-through sea water. All four groups were acclimatized to sea water for 3 weeks and fed a diet of minced fresh seafood. After 3 weeks the groups were fed either; seafood as before, a different commercial pelleted diet associated with the development of GDAS on farms, or either diet supplemented with 500 mg L(-1) putrescine, 300 mg L(-1) cadaverine and 250 mg L(-1) tyramine. Gastric dilation was produced in fish fed the commercial diet for 1 month but not by feeding a diet of minced seafood. The addition of putrescine, cadaverine and tyramine to either diet had no significant effect on the development of gastric dilation. Fish fed the commercial diet had significantly (P < 0.0001) wider weight-adjusted stomach widths, less prominent longitudinal stomach folds (P < 0.0001) and lower (P < 0.0001) stomach-width ratios than fish fed the fresh seafood diet. There was no significant difference in serum osmolality or sodium concentration between fish from groups with or without gastric dilation or fed biogenic amines.  相似文献   

Free polyamine concentrations in coastal seawater during phytoplankton bloom   总被引：1，自引：0，他引：1  

Naoyoshi Nishibori  Akihiko Yuasa  Motosuke Sakai  Shinsuke Fujihara  Sachio Nishio 《Fisheries Science》2001,67(1):79-83

SUMMARY: Polyamines are widely distributed in nature and known to have many roles in living organisms. We investigated the concentrations of polyamines together with inorganic nutrients during a summer bloom period in the Seto Inland Sea of Japan. Putrescine and spermidine were the major polyamines in the coastal seawater. The concentrations at 1 m depth varied widely during the sampling period and ranged from 2.0 to 32.6 nM and 1.0 to 14.1 nM. Spermine concentrations were much lower than putrescine and spermidine. In addition, other polyamines (diaminopropane, cadaverine, norspermidine, homospermidine, norspermine) were also detected. Putrescine and spermidine seemed to be significant compounds in dissolved organic nitrogen in coastal seawater.  相似文献   

腐胺引发对2个转基因抗虫杂交棉耐盐性的影响   总被引：1，自引：0，他引：1  

刘天雄  陈进红  何秋伶  祝水金 《棉花学报》2013,25(5):446-452

以转基因抗虫杂交种慈抗杂3号和浙杂14为材料,研究了棉花种子不同浓度腐胺引发对于转基因抗虫杂交种在0.5％盐胁迫条件下的发芽、出苗、产量和纤维品质的影响.研究结果,腐胺引发处理可以显著地提高转基因抗虫杂交棉种子在盐胁迫条件下的发芽率、发芽指数和田间出苗率,显著降低平均发芽时间.在含有0.5％盐的沿海滩涂地上,腐胺引发处理后的单株铃数、铃重和衣分无显著差异,但最后的皮棉产量却有显著增加,并可提高纤维的马克隆值和比强度.腐胺处理对非盐胁迫条件下的幼苗体内的SOD活力、POD活力和MDA含量无显著影响,但在盐胁迫条件下可显著或极显著地提高转基因抗虫杂交棉幼苗的体内的SOD与POD活力,显著减少MDA的积累.  相似文献   

腐胺对金矮生苹果坐果及花粉萌发的影响     

孙丽文  史常青  李建聪  谷吉斌 《内蒙古林业科技》2012,38(3):5-7

金矮生苹果是金冠苹果的短枝型芽变,树体矮小,适宜密植,单位面积产量高,经济效益好。但在辽西半干旱地区粗放管理条件下,大小年结果现象明显。国内外资料表明,腐胺对多种果树的坐果及花粉萌发有促进作用。通过试验,我们提出了相应的技术措施。  相似文献   

Interacted Effect ofArbuscular Mycorrhizal Fungi and Polyamines on Root System Architecture of Citrus Seedlings     

WU  Qiang-sheng ZOU  Ying-ning LIU  Chun-yan LU  Ting 《中国农业科学(英文版)》2012,(10):1675-1681

  相似文献