可可泛素结合酶基因家族的生物信息学初步分析     

赵秋芳  董晨  决登伟  陈宏良  贾利强 《热带作物学报》2016,37(9):1732-1740

泛素结合酶(E2s)促进底物泛素化或者与E3s链接,是靶蛋白泛素化的关键酶,在泛素-蛋白酶体途径中起重要作用。利用可可(Theobroma cacao L.)全基因组测序数据,共鉴定出45个E2s基因家族成员,包括39个UBC和6个UEV基因。通过生物信息学方法,对可可E2s家族的基本理化性质、基因结构、二级结构预测、亚细胞定位、进化关系等方面进行初步分析。结果表明:可可E2s基因CDS长度在441(Tc UEV3)~3 651 bp(Tc UBC7)之间,对应的编码蛋白氨基酸数目在146(Tc UEV3)~1 216 aa(Tc UBC7)之间,编码蛋白分子量在16.39~134.71 ku之间。E2s基因外显子在1~11之间,多数基因外显子数目在5~7之间。E2s基因在10条染色体上均有分布,1号染色体为7个,数量最多;6号染色体2个基因,数量最少。可可E2s蛋白大多为不稳定蛋白,且均为亲水性蛋白,其二级结构以α-螺旋和无规则卷曲为主要构成元件。大多数可可E2s蛋白定位在细胞核,少数定位在内质网或者细胞质。进化树分析表明:可可E2s蛋白被分为20个亚家族,包括16个UBC亚家族和4个UEV亚家族,第Ⅵ亚家族E2s数目最多为9个;E2s家族蛋白在物种进化过程中具有高度保守性。  相似文献   

超高效液相色谱串联质谱法对水产品中硝基呋喃代谢物的测定     

王狄  张嘉楠 《河北渔业》2016,(7):47-51

建立了使用Waters UPLC Xevo TQ超高效液相色谱串联质谱仪检测水产品中4种硝基呋喃代谢物的分析方法。匀浆后的样品在酸性环境下水解,并用2-硝基苯甲醛衍生16h,经磷酸氢二钾提取,LMS固相萃取小柱净化后,在三重四极杆多反应监测模式下进行测定。方法采用同位素内标法定量,四种代谢物的检出限(LOD)均低于0.25μg·kg-1,线性范围在0.5μg·L~(-1)到10μg·L~(-1)间,相关系数大于0.99。在0.5、1.0和5μg·kg-1浓度下分别进行添加回收试验(n=5),回收率在75.2%~123.3%之间,相对标准偏差(RSD)小于9.4%。  相似文献   

仙草多糖提取工艺条件优化研究     

林慧霞  肖玉娟  黄华斌  傅奇  何少贵 《热带作物学报》2016,(4):22-26

采用水提法浸提仙草，以仙草多糖的提取率为指标，研究料液比、浸提温度、浸提时间及浸提次数4个因素对其提取率的影响，并从中挑取合适的因素水平进行正交试验及SPSS分析。结果表明：在这4个因素中，对提取率影响最大的因素是浸提温度，然后是浸提时间，再次是料液比，最后是浸提次数；在料液比1:60、温度100 ℃、浸提时间3 h的最优条件下，仙草多糖的提取率高达17.89 %，此时仙草多糖的含量为44.13 %。  相似文献   

An improved method for extraction of sorghum polymeric protein complexes     

《Journal of Cereal Science》2020

A method for fractionating sorghum proteins using extraction solvents and techniques designed to obtain polymeric protein structures (especially disulfide linked) was developed. Extraction and separation conditions were optimized in terms of completeness of protein extraction, sample stability, and analytical resolution. After pre-extraction of albumins and globulins, a 3-step sequential procedure involving no reducing agents was applied to ground whole sorghum flour. The three fractions obtained represented proportionally different protein polymer contents and molecular weight distribution as evidenced by comparative size exclusion chromatography. Protein composition also varied among the extracts with differences in kafirin composition and non-kafirin proteins detected in the fractions by RP-HPLC and SDS-PAGE analysis. The ability to quantify and further characterize sorghum polymeric protein complexes will be useful for additional studies linking protein structures with functionality and digestibility and variations for these properties within diverse sorghum germplasm.  相似文献   

裂褶菌多糖的研究进展     

贺凤  黄龙花  刘远超  李诚斌  胡惠萍 《食用菌学报》2016,(2):88-93

裂褶菌多糖(Schizophyllan)是裂褶菌(Schizophyllum commune)的主要活性成分之一.综述了裂褶菌多糖结构、提取纯化工艺和生理活性功能等方面的研究进展.  相似文献   

Dysbiotic gut microbiome: A key element of Crohn's disease     

《Comparative immunology, microbiology and infectious diseases》2015

Since the first publication on “regional ileitis”, the relevance of this chronic inflammatory disease condition termed finally as Crohn's disease is continuously increasing. Although we are beginning to comprehend certain aspects of its pathogenesis, many facets remain unexplored. Host's gut microbiota is involved in a wide range of physiological and pathological processes including immune system development, and pathogen regulation. Further, the microbiome is thought to play a key role in Crohn's disease. The presence of Crohn's-associated variants of NOD2 and ATG16L genes appears to be associated not only with alterations of mucosal barrier functions, and bacterial killing, but the gut microbiota, as well, reflecting a potential relationship between the host's genotype and intestinal dysbiosis, involved in disease etiology. This review aims to characterize some exciting new aspect of Crohn's disease pathology, focusing mainly on the role of intestinal microbes, and their interplay with the immune system of the host.  相似文献   

地稔凝集素的提取及凝血活性的研究     

邓政东  程爱芳  李秀丽 《黑龙江农业科学》2015,(2):56-58

为研究和利用地稔的促凝血活性物质,以地稔为试材,提取地稔凝集素,研究pH、温度和糖等理化因素对凝血活性的影响。结果表明:经硫酸铵沉淀及透析提取的地稔凝集素具有红细胞凝集活性;碱性条件下,抑制作用明显,而酸性条件有较强促进作用;温度对凝集素凝血活性影响很小;半乳糖有明显促进作用。  相似文献   

Relationship between corn yield and chemical composition of the ear leaf measured with different regression models     

《Communications in Soil Science and Plant Analysis》2012,43(3):123-130

Abstract

Corn yields and leaf samples were obtained from experimental plots receiving varying rates of N, P and K. Yields were regressed on leaf levels of N, P, K, Ca, Mg, B, Cu, Fe, Mn and Zn as independent variables. Various polynomial regressions were fitted to the yields and “goodness of fit”; of particular mathematical models was used as a basis for evaluating particular biological and statistical concepts.

It was found that no regression, where chemical elements were used as ratios, fit the observations as well as a quadratic polynomial or its square root transformation. This suggests that within the set of data used, emphasis on particular cation or anion ratios does not necessarily result in the “best”; explanation of variation in yield.

“Classical”; growth equations were fitted to corn yields, but were not as precise in predicting yields as the polynomials. A modified stagewise regression procedure was used for fitting one mathematical model, but results were less satisfactory than those obtained with usual least squares procedures.  相似文献   

Importance of Noncrystalline Hydroxide Phases in Sequential Extractions to Fractionate Antimony in Acid Soils     

《Communications in Soil Science and Plant Analysis》2012,43(11-12):1487-1501

Abstract

Sequential extraction techniques have been used to make inferences about speciation of phosphorus (P) and to a lesser extent arsenic (As) in soils. However, sequential extraction studies on the less‐abundant group V element, antimony (Sb), are limited. In this work, a widely used P sequential extraction scheme was modified and used to extract P, As, and Sb from two acidic soils from the Macleay River floodplain, NSW, that were enriched with Sb (26.9 and 23.0 mg kg^?1). An ammonium oxalate–oxalic acid step was included in the extraction sequence to dissolve the noncrystalline iron (Fe) and aluminium (Al) hydroxide phase. It extracted 30 to 47% of Sb, indicating the importance of this fraction, which may be mobilized in the floodplain by acid sulfate soil processes and periodic waterlogging. The original method overestimated P, As, and Sb in the residual fraction (30–71%). Relative efficiency values of extracts for P, As, and Sb were compared, and inferences about phase distributions were made. The results suggest some potential in using extractions to assess bioavailability of Sb in soil.  相似文献   

Genetics of equine bleeding disorders     

Anna R. Dahlgren  Fern Tablin  Carrie J. Finno 《Equine veterinary journal》2021,53(1):30-37

Genetic bleeding disorders can have a profound impact on a horse's health and athletic career. As such, it is important to understand the mechanisms of these diseases and how they are diagnosed. These diseases include haemophilia A, von Willebrand disease, prekallikrein deficiency, Glanzmann's Thrombasthenia and Atypical Equine Thrombasthenia. Exercise-induced pulmonary haemorrhage also has a proposed genetic component. Genetic mutations have been identified for haemophilia A and Glanzmann's Thrombasthenia in the horse. Mutations are known for von Willebrand disease and prekallikrein deficiency in other species. In the absence of genetic tests, bleeding disorders are typically diagnosed by measuring platelet function, von Willebrand factor, and other coagulation protein levels and activities. For autosomal recessive diseases, genetic testing can prevent the breeding of two carriers.  相似文献