Alternative Route of Infection for Bacterial Seedling Blight of Rice Caused by Burkholderia plantarii     

Hisayoshi MIYAGAWA  Hiroyoshi INOUE 《Journal of General Plant Pathology》2002,68(4):356-362

Burkholderia plantarii , the pathogen of bacterial seedling blight of rice, was detected in paddy water. Its concentration rose in July and August. The bacterial concentration in the paddy water was always higher along levees than at distances more than 5 m from levees. Confirmed to be released into water when graminaceous weeds were immersed, B. plantarii survived for at least 4 days at 30°C. B. plantarii was splashed at least 30 cm upward by rain splash in the field. Harvested seeds, which had been sprayed with B. plantarii released from graminaceous weeds at the flowering stage, retained the bacteria. Bacterial seedling blight occurred when the seeds were then sown in nursery boxes. These results indicated that graminaceous weeds growing on levees of paddy fields are a source of infection of the disease and that rice seeds are infected through the paddy water. Received 23 May 2002/ Accepted in revised form 1 September 2002  相似文献   

Observations of Phytophthora spp. in Water Recirculation Systems in Commercial Hardy Ornamental Nursery Stock     

Karin Themann  Sabine Werres  R. Lüttmann  H.-A. Diener 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(4):337-343

Samples of water and sediment were taken from drains, reservoirs and wells from four commercial hardy ornamental nurseries with water recirculation systems. The samples were taken on seven different dates throughout a single year from August 1994 to July 1995. The samples were screened for Phytophthora species using five different methods: direct plating, three bait tests (using lupin seedlings, apples and Rhododendron leaves) and a DAS-ELISA (double-antibody sandwich enzyme-linked immunosorbent-assay) with two antisera. In the nurseries with old water recirculation systems, Phytophthora species were detected in the drains and in the reservoirs. In the nursery with a new recirculation system, the pathogens were only present in the drains. None of the water samples from wells in any of the nurseries were contaminated. Phytophthora species were present in the water as well as in the sediment samples from drains and reservoirs. They were detected in the water recirculation systems irrespective of the season. The number of isolates increased about sevenfold between late summer and spring. At least 12 different Phytophthora species were identified: some isolates were previously unrecorded species. The epidemiology of the pathogens in outdoor water recirculation systems as well as the importance of the results for commercial nurseries is discussed.  相似文献   

Variation in the development of secondary dormancy in oilseed rape genotypes under conditions of stress   总被引：6，自引：0，他引：6  

E J J Momoh  W J Zhou  & B Kristiansson† 《Weed Research》2002,42(6):446-455

Summary A substantial amount of seed is left in the fields before and during harvest of oilseed rape. Although this crop exhibits little or no primary dormancy, the absence of certain environmental cues that promote germination of imbibed seeds induces secondary dormancy. The work reported investigated the extent to which environmental stress conditions, including osmotic stress, low oxygen stress and anaerobiosis, induce secondary dormancy in oilseed rape, and examined the variation in development of secondary dormancy between and within genotypes. Osmotic stress was most effective in inducing dormancy. Anaerobic treatment produced very few dormant seeds, as did an atmosphere low in oxygen and high in nitrogen. The development of secondary dormancy under osmotic stress varied considerably between and within genotypes. Dormancy ranged from almost zero to about 60% for winter genotypes and about 85% for spring types. Within genotypes, variations occurred between seed lots and years of harvest. Temperature variations affected the percentage of dormant seeds. More dormant seeds were likely to be produced with incubation under water stress at 20 °C than at 12 °C. In winter genotypes, fewer dormant seeds were produced when incubation temperature and germination test temperatures differed. Thus, incubating at 20 °C and 12 °C, followed by germination tests at 20 °C and 12 °C, respectively, produced most dormant seeds. Also, in the winter genotypes, the potential development of secondary dormancy was positively correlated with the pattern and speed of germination of untreated seeds.  相似文献   

Detection of Colletotrichum coccodes from soil and potato tubers by conventional and quantitative real-time PCR   总被引：4，自引：1，他引：4  

D. W. Cullen  A. K. Lees  I. K. Toth  J. M. Duncan † 《Plant pathology》2002,51(3):281-292

Colletotrichum coccodes is the causal agent of the potato blemish disease black dot. Two PCR primer sets were designed to sequences of the ribosomal internal transcribed spacer (ITS1 and ITS2) regions for use in a nested PCR. The genus-specific outer primers (Cc1F1/Cc2R1) were designed to regions common to Colletotrichum spp., and the species-specific nested primers (Cc1NF1/Cc2NR1) were designed to sequences unique to C . coccodes . The primer sets amplified single products of 447 bp (Cc1F1/Cc2R1) and 349 bp (Cc1NF1/Cc2NR1) with DNA extracted from 33 European and North American isolates of C. coccodes. The specificity of primers Cc1NF1/Cc2NR1 was confirmed by the absence of amplified product with DNA of other species representing the six phylogenetic groups of the genus Colletotrichum and 46 other eukaryotic and prokaryotic plant pathogenic species. A rapid procedure for the direct extraction of DNA from soil and potato tubers was used to verify the PCR assay for detecting C. coccodes in environmental samples. The limit of sensitivity of PCR for the specific detection of C. coccodes when inoculum was added to soils was 3·0 spores per g, or the equivalent of 0·06 microsclerotia per g soil, the lowest level of inoculum tested. Colletotrichum coccodes was also detected by PCR in naturally infested soil and from both potato peel and peel extract from infected and apparently healthy tubers. Specific primers and a TaqMan fluorogenic probe were designed to perform quantitative real-time (TaqMan) PCR to obtain the same levels of sensitivity for detection of C. coccodes in soil and tubers during a first-round PCR as with conventional nested PCR and gel electrophoresis. This rapid and quantitative PCR diagnostic assay allows an accurate estimation of tuber and soil contamination by C. coccodes .  相似文献   

Effect of submergence and naproanilide application on the growth of hemp sesbania and rice     

Kangetsu Hirase  William T. Molin 《Weed Biology and Management》2002,2(2):116-119

  相似文献   

黑河干流中游地区耗水量变化的历史分析   总被引：9，自引：6，他引：9  

任建华  李万寿  张婕 《干旱区研究》2002,19(1):18-22

黑河流域水资源极其匮乏 ,加之地区上的配置不均衡和中游地区水土资源的过度开发导致了黑河下游严重生态环境问题 ,已影响到我国北方广大地区生态环境质量 ,受到国家的高度重视和全社会的广泛关注。黑河水资源开发利用主要集中在中游地区 ,该区是全国重要的商品粮、蔬菜和经济作物生产基地之一 ,耗水量占流域耗水量的 85 %以上 ,是黑河的主要耗水区和径流利用区。本文利用黑河上游控制站莺落峡站和中游控制站正义峡站水文资料 ,分析了黑河中游耗水量的变化 ,结果表明 :2 0世纪 80年代以前黑河中游耗水量在 4.2 0× 10 8m3 左右 ,比较稳定 ;进入 2 0世纪 80年代以来耗水量明显增加 ,80年代耗水量比 40年代末期至 70年代增加了 2 .0× 10 8～ 2 .5×10 8m3 左右 ;90年代耗水量又比 80年代增加了 1.6 0× 10 8m3 左右。 80年代以来流域耗水量呈明显增加的趋势 ,这是以人类活动影响为主的结果  相似文献   

黑河流域荒漠地区梭梭人工林地土壤水分动态研究   总被引：29，自引：7，他引：29  

刘发民  张应华  仵彦卿  张小军 《干旱区研究》2002,19(1):27-31

梭梭林地土壤剖面的含水量与裸沙地含水量显著不同 ,裸沙地土壤含水量随深度增加而增加 ,林地在 30～ 12 0cm范围内有较高的含水量 ;林地 0～ 30cm的上层土壤含水量受降雨及蒸发的剧烈影响 ,30～ 2 0 0cm范围内的林地土壤各测量层平均含水量与其标准差有二次函数的关系 ;降雨对每月的 0～ 2 0 0cm的土壤贮水量没有明显的影响 ,说明降雨多数在当月就被蒸散和深层渗漏所消耗 ;认为梭梭人工林这种脆弱生态系统能够在严酷的环境条件下存在 ,其根系对土壤水和地下水有吸水和释水的再分配功能是重要的因素之一  相似文献   

农田沙漠化演变中土壤质量的生物学特性变化   总被引：9，自引：5，他引：9  

苏永中  赵哈林  张铜会  文海燕 《干旱区研究》2002,19(4):64-68

通过研究处于沙漠化过程中的科尔沁沙地旱作农田土壤酶活性与基础土壤呼吸活性，分析了土壤质量生物学特性的沙漠化演变。结果表明，土壤酶活性与基础土壤呼吸对沙漠过程中环境的变化反应敏感，随着沙漠化程度增加，与土壤物理化学肥力因素有关的过氧化氢酶、脲酶和碱性磷酸酶的活性和土壤呼吸活性随之下降。土壤酶活性和呼吸活性与土壤有机质和养分有极显著的相关，在一定程度上可以用酶活性和呼吸强度表征有机质和养分的状况及其数量的变异，因此，可以作为农田沙漠化演变中土壤质量特征变化的重要辅助指标。  相似文献   

孔雀河流域绿洲生态支持系统调控模式研究   总被引：8，自引：0，他引：8  

王铁成  谢红彬  贾宝全 《干旱区资源与环境》2002,16(3):7-11

生态环境用水量的减少是孔雀河流域近 50年来现代绿洲环境退化的主要原因 ,维持生态与社会经济的合理用水比例是绿洲系统持续发展的前提。以此为基础 ,本文采用水资源承载力的方法探讨了绿洲生态支持子系统和社会经济子系统优化调控模式 ,最后就配置方案及发展模式的可行性进行了分析  相似文献   

民勤绿洲生态气候资源及其利用   总被引：4，自引：0，他引：4  

韩福贵  王键 《干旱区资源与环境》2002,16(3):51-56

民勤绿洲边缘 ,光照丰富、温差大 ,有利于农产品质量的提高。但是水资源缺乏 ,地下水位急剧下降和土地沙化是民勤绿洲农业生产的首要问题。如何充分利用现有生态资源 ,并充分提高其利用率是摆在我们面前的一个主要课题。只有通过引进优良品种 ,调整农业种植结构 ,大力推广节水灌溉技术 ,才是解决上述问题的有效途径  相似文献