花粉多糖注射剂质量标准中几个项目测定     

王开发  支崇远  陆明 《蜜蜂杂志》2004,(3):3-4

根据中国药典对注射剂的要求,对花粉多糖的蛋白质、鞣质、树脂、草酸盐、炽灼残渣、钾离子、砷盐、重金属含量进行测定,均符合规定,达到质量标准。  相似文献   

花粉多糖注射剂临床前抗肿瘤药效学预试   总被引：4，自引：1，他引：4  

王开发  支崇远  严惠芳  陆宏祺  卜华祥  左其芳 《蜜蜂杂志》2004,(6):3-4

花粉多糖对小鼠Lewis肺癌(足趾接种)模型以200mg/kg,100mg/kg,50mg/kg三个剂量iv×10qd方案给药,实验显示高、中、低三个剂量组抑瘤率分别为48.01%,43.09%,31.38%。免疫实验以上述相同的给药方案,花粉多糖对荷Lweis肺癌小鼠NK细胞活性具有明显的提高和促进作用。  相似文献   

淡紫拟青霉发酵滤液对大豆胞囊线虫趋化性的影响   总被引：5，自引：0，他引：5  

孙漫红  刘杏忠 《植物病理学报》2004,34(4):376-379

 实验室条件下,研究了大豆胞囊线虫幼虫对大豆根和根浸出液的趋化性以及淡紫拟青霉代谢产物对其趋化性的影响。结果表明,大豆胞囊线虫二龄幼虫(J₂)对大豆根存在着一定的趋化性,而淡紫拟青霉发酵滤液对J₂存在明显的驱避性。在根浸出液、真菌滤液及其混合液剂处理下,大豆胞囊线虫J₂在WA平板上靠近处理液的0~1cm区间中分布率存在极显著差异(P <0.0 1),而加样时间(液剂与线虫同时放置或提前2 4h处理)对其分布率没有显著影响。大豆幼根蘸取滤液后对线虫J₂在该区间的分布与不经处理的幼根差异显著(P <0.0 5),证明淡紫拟青霉发酵滤液可以明显地降低线虫与大豆根的亲和力,并强烈抑制线虫对大豆根的侵染。  相似文献   

UHPLC-Q/TOF MS结合HPLC-DAD定性和定量分析硫酸黏菌素可溶性粉中的未知添加物     

陆春波  包爱情  周芷锦  张航俊  陈慧华 《中国兽药杂志》2018,52(12):54-60

定性和定量分析一批兽药硫酸黏菌素可溶性粉中的未知添加物。照《中国兽药典》2010年版一部对该批检品用微生物检定法进行含量测定时,发现该样品的抑菌圈为虚圈,用薄层色谱鉴别该样品,未显示与标准品溶液一致的主斑点,怀疑该样品中有处方外非法添加物。采用超高效液相色谱-四级杆-飞行时间质谱(UHPLC-Q/TOF MS)对该样品进行筛查,发现疑似添加物,并使用液相色谱-二极管阵列检测(HPLC-DAD)法进行了双重确证和含量测定。该样品中非法添加物确证为磺胺氯达嗪和甲氧苄啶,添加量分别为58.5 mg/g和13.4 mg/g。本研究通过建立筛查方法为监管部门提供技术支撑,通过分析非法添加物的可能原因为打击兽药处方外非法添加提供了思路。  相似文献   

Processing traits and digestibility of extruded dog foods with soy protein concentrate          下载免费PDF全文

K. S. Venturini  M. F. Sarcinelli  M. A. Baller  T. C. Putarov  E. B. Malheiros  A. C. Carciofi 《Journal of animal physiology and animal nutrition》2018,102(4):1077-1087

Soya bean protein concentrate (SPC) with two particle sizes were evaluated on extrusion parameters, kibble formation, digestibility and palatability of dog foods. Eight diets were extruded: PBM—control diet based on poultry by‐product meal (PBM); GM—a diet in which corn gluten meal (GM) replaced 45% of the diet protein; cSPC15%, cSPC30% and cSPC45%—diets in which SPC of coarse particle size (600 μm) replaced 15%, 30% and 45% of the diet protein; and sSPC15%, sSPC30% and sSPC45%—diets in which SPC of small particle size (200 μm) replaced 15%, 30% and 45% of the diet protein. The digestibility of nutrients was evaluated for the PBM, GM, cSPC45% and sSPC45% diets, using six dogs per food. The PBM, GM and cSPC45% diets were compared for palatability. Data were submitted for analysis of variance, and the means were compared by polynomial contrasts or Tukey's test (p < .05). The cSPC increased the specific mechanical energy (SME) application, extrusion temperature and pressure linearly, resulting in lower kibble density and higher expansion and starch gelatinization (SG) (p < .01). When comparing the PBM, GM, cSPC45% and sSPC45% diets, higher SME, extrusion temperature and pressure, SG and kibble expansion were verified for the cSPC45% diet (p < .05). The DM, fat and crude protein digestibility were similar among diets. Faecal pH, ammonia and lactate did not differ, demonstrating that the removal of oligosaccharides and soluble non‐starch polysaccharides of SPC produces an ingredient with mostly non‐fermentable fibre. Dogs preferred the PBM to the GM diet (p < .05), but consumed the PBM and cSPC45% foods equally. In conclusion, SPC exhibited good extrusion functionality, favouring kibble expansion and SG, with high digestibility, similar to that of PBM. The removal of soluble compounds from soya beans resulted in an ingredient with low fermentable fibre content, which did not alter faecal formation or characteristics.  相似文献   

多糖提取及对家禽生长、免疫和产蛋性能影响研究进展     

欧蜀云  欧永春  姚长红 《饲料研究》2021,(4):141-143

多糖是由多个单糖通过糖苷键结合而成的高分子化合物。食用菌及植物的组织中均存在丰富的天然多糖类物质。多糖具有抗氧化、提高免疫力、改善肠道健康等多种生理功能,作为功能性添加剂在饲料中被广泛应用。文章对多糖的提取方法及对家禽生长、免疫、产蛋性能影响进行综述,为进一步获取优质多糖及在饲料中的应用提供参考。  相似文献   

Development New Molecular Markers for Quantitative Trait Locus (QTL) Analysis of the Seed Protein Content Based on Whole Genome Re-Sequencing in Soybean     

WANG Jia  ZENG ZhaoQiong  LIANG JianQiu  YU XiaoBo  WU HaiYing  ZHANG MingRong 《中国农业科学》2019,52(16):2743-2757

  相似文献   

Glycine max and Glycine soja are capable of cold acclimation          下载免费PDF全文

J. Robison  N. Arora  Y. Yamasaki  M. Saito  J. Boone  B. Blacklock  S. Randall 《Journal of Agronomy and Crop Science》2017,203(6):553-561

Soybean has been considered a cold intolerant species; based largely upon seed germination and soil emergent evaluations. This study reports a distinct acquisition of cold tolerance, in seedlings, following short acclimation periods. Diversity in cold responses was assessed in eight cultivars of Glycine max and six accessions of G. soja. All varieties of soybean significantly increased in freezing tolerance following acclimation. This study indicates soybean seedlings are indeed capable of sensing cold and acquiring cold tolerance. Germination rates after cold imbibition were negatively correlated with maturity group, but positively correlated with cold acclimation potential in G. soja. Seed fatty acid composition was varied between the species, with Glycine soja accessions containing about 2‐times more linolenic acid (18:3) than G. max. Furthermore, high levels of linoleic acid (18:2) in seeds were positively correlated with germination rates following cold imbibition in G. soja only. We suggest that domestication has not impacted the overall ability of soybean to cold acclimate at the seedling stage and that there is little variation within the domesticated species for ability to cold acclimate. Thus, this brief comparative study reduces the enthusiasm for the “wild” species as an additional source of genetic diversity for cold tolerance.  相似文献   

大豆GmNRT1.2a和GmNRT1.2b基因的克隆及功能探究     

LI Guo-Ji  ZHU Lin  CAO Jin-Shan  WANG You-Ning 《作物学报》2020,46(7):1025-1032

拟南芥中硝酸盐的吸收、转运和分配是通过硝酸盐转运蛋白(nitrate transporter, NRT)实现的。尽管之前的生物信息学分析推测大豆GmNRT1.2s可能参与共生固氮过程,但尚未开展相应的功能研究。本研究通过对其表达模式分析収现,GmNRT1.2a和GmNRT1.2b分别在根和叶中高表达,且受硝酸盐诱导,在接种根瘤菌与结瘤因子(nodfactors,NFs)后表达量明显升高。功能研究结果显示,过表达GmNRT1.2a或GmNRT1.2b后大豆根瘤数目显著增加。本研究为深入探究GmNRT1.2a和GmNRT1.2b调控大豆共生固氮过程的分子机制提供了一定的数据支持。  相似文献   

6种大孔树脂对复方免疫增强剂多糖的吸附解吸性能     

李赞阳  李炳奇  刘红  龚晓武  谷新利 《西北农业学报》2011,20(12):16-18

筛选出对中药复方免疫增强剂多糖具有较好的吸附解吸效果的大孔树脂。通过静态吸附及解吸试验、动态吸附及解吸试验，利用苯酚 硫酸法跟踪检测多糖质量浓度，以吸附率与解吸率为评价指标，初步研究AB 8、LSA 5B、DM 18、CAD 40、LX 1、D941型大孔树脂对中药复方免疫增强剂中多糖的吸附及解吸性能。结果表明，在6种大孔树脂中， AB 8对中药复方免疫增强剂多糖的动态吸附率和解吸率最高，分别为54%和90%。说明AB 8型大孔树脂可以作为分离纯化多糖的材料。  相似文献