RFLPs of chloroplast and mitochondrial DNA in wild soybean, Glycine soja, growing in China     

Yoshiya Shimamoto  Hirofumi Fukushi  Jun Abe  Akira Kanazawa  Junyi Gai  Zhong Gao  Donghe Xu 《Genetic Resources and Crop Evolution》1998,45(5):433-439

RFLPs of chloroplast (cp) and mitochondrial (mt) DNA have been used to characterize the cytoplasmic genome of wild soybean, Glycine soja, growing in China. We have found from the RFLPs of that the cytoplasmic genomes of most Chinese wild soybeans have a combination of cpIII with mt-a or a combination of cpII with mt-b accompanied by mtIV or mtV according to our classification. CpII was not observed in combination with mt-a and cpIII was not observed in combination with mt-b. The regional distribution of these two types showed clines with opposite directions. The Yangtze River Valley had the greatest diversity in each of cp and two mt profiles detected in this study and the cytoplasmic genome combining these three profiles showed the highest degree of polymorphism in this region. The Yangtze River Valley may be a center of cytoplasmic diversity of wild soybean and may contain various genetic resources of soybean.  相似文献   

应用~(15)N稀释法筛选高固氮能力的大豆品种   总被引：3，自引：0，他引：3  

李海先  李新民  Seth K.A.Danso 《核农学报》1998,12(5):0-0

应用＾１５Ｎ稀释法筛选出了具有高固氮能力的大豆品种和品系,大豆品系１００５,８５０２,２０９６,１４５４,９４用东农４２是高固氮品系,它们的固氮效率高达７０％,大豆品系１４５４和１５５５即高产又高固氮。根据方差分析,不同大豆品种或品系固氮效率差异显,固氮效率与产量无相关关系,与成熟期呈正相关,成熟期越长,固氮效率越高。有１０年品种或品系在１９９２和１９９４年两次进行试验,尽管两年的降雨情况有较大  相似文献   

利用高能液相色谱测定根际分泌物中的有机酸: Ⅲ.干扰因子的影响     

SHEN Jian-Bo  ZHANG Fu-Suo  HUANG Qin  MAO Da-Ru 《土壤圈》1998,8(2):97-104

Methods for determining niene low molecular-weight oragnic acids in root exudates were developed by using reversed-phase high performance liquied chromatography with UV (ultraviolet) detection at 214 nm. The mobile phase was 18 mmol L^-1 kH2PO4 adjusted to pH 2.25 with phosporic acid nd the flow rate was 0.3 mL min^-1,The analytical column was a reversed-phase silica based C-18 column( shim-pack CLC-ODS).The root exudates were collected through submerging the whole root system into aerated deionized water for 2 hours ,The filtered exudate solutions were concentrated to dryness by rotary evaporation at 40℃,dissolved in 10 mL mobile phase.The chromatoraphic conditions of organic acid determination were analyzed.The results showed that there was a high selectivity and sensitivity in the organic acid determination by reversed-phase high performance liquid chromatography.Coefficients of variation for organic acied determination were lower than 10% except lactic acid .The recoveries were consistently between 80.1% to 108.3% .Detection limits were approximately 0.05 to 4.5 mg L^-1 for organic acids except succinic acid with the detection limit of 7.0 mg L^-1 .Phosphorus deficiency may contribute to the release of organic acids in soybean root exudates especially malic,lactic and citric acids.  相似文献   

小麦午睡原因的研究——Ⅲ.形成小麦午睡生态生理因素作用的综合分析   总被引：4，自引：0，他引：4  

韩凤山  赵明  赵松山  王美云 《作物学报》1988,14(4):296-302

结合田间生态条件研究了各因素在形成小麦午睡中的作用。并且在人工控制的恒定适温、恒定 CO_2浓度、相对稳定的叶片水势和叶片可溶性醣含量的条件下基本上克服了午睡。研究认为高产麦田 CO_2浓度下降、叶片水势下降以及其所引起的气孔开度减低是形成午睡的主要原因;超过小麦光合适温的高气温和叶片可溶性醣含量过量积累是形  相似文献   

吉林省小粒大豆与大粒大豆某些品质性状的比较研究   总被引：8，自引：2，他引：6  

蔚荣海  雷耔耘 《吉林农业大学学报》1998,20(3):12-16

对吉林省小粒大豆进行了多项生化品质成分分析研究,结果表明;１．小粒大豆品种在蛋白质及氨基酸含量上均高于大粒大豆品种,平均分别高出２．８２％和１．５４％;脂肪含量低于大粒大豆各品种,但小粒大豆的脂肪酸组成中,亚油酸含量较高,比大粒大豆平均高出５．０９％,小粒大豆亚油酸与亚麻酸配比合适;２．小粒大豆的总糖含量低于大粒大豆,其还原糖含量却高于大粒大豆,平均高出０．６１％;小粒大豆各品种粗纤维一高于大粒大  相似文献   

Study on the Yield Reduction Mechanism of Soybean Planted under Continuous and every Second Year Cropping Conditions   总被引：6，自引：0，他引：6  

ZuWei  LiuZhongtang 《东北农业大学学报(英文版)》1998,5(2):81-95

Three-year investigation was conducted to demonstrate the mcchanism of reduction of soybean yield aroused by continuous and every second year cropping.Compared to normal cropping,there are many unfavorable changes in some major elements of soybean plant and soil environmeng.Chloropyll content was .Phosphorous content of soybean plant was decreased seriously.Potassium content was lower while calcium content was higher.Magnesim content was wot affected and decreased in susceptive varieties.Some deseases and insects of soybean under continuous and very second year cropping conditions became serious as continuous years prolonged.Organic matter content tended to go down from normal rotation to continuous cropping .Amount of bacteria and antinomyces decreased while amount of fungi increased.The evelopment of symbiotic nitrogen fixation system was deteriorated.  相似文献   

磷胁迫下大豆根分泌有机酸的动态变化   总被引：4，自引：0，他引：4  

申建波  张福锁  毛达如 《中国农业大学学报》1998,(Z3)

通过水培试验采用直接收集法和高效液相色谱技术测定了大豆根分泌物中有机酸的组成和含量,探讨了不同磷处理条件下大豆根分泌物中有机酸的动态变化,分析了不同磷处理对大豆根系生长和根分泌有机酸的影响。结果表明,大豆缺磷时,根冠比和根长密度增加,根直径变小,根分泌物中有机酸特别是苹果酸、柠檬酸和反丁烯二酸的数量增加,缺磷处理苹果酸和顺丁烯二酸的释放高峰先于供磷处理,不同生育时期有机酸分泌量存在明显差异。  相似文献   

磷素营养与大豆生长和共生固氮的关系   总被引：3，自引：0，他引：3  

丁洪  李生秀 《西北农林科技大学学报(自然科学版)》1998,26(5):67-70

在水培条件下,研究了磷素营养对２个大豆品种生长和共生固氮的影响。结果表明,水培液中磷含量在２．３～９．３ｍｇ／Ｌ时,２个大豆品种的植株生长量、根瘤数、根瘤干重、固氮活性和氮积累量均随磷浓度增加而显著增加;而在９．３～３７．０ｍｇ／Ｌ时则大大降低。磷浓度过高会造成毒害,但植株的磷积累量却与施磷量呈显著的线性回归关系。植株不同部位磷含量是根瘤＞根＞地上部分;氮含量是根瘤＞地上部＞根。２个大豆品种对磷肥反应有显著差异。  相似文献   

改变普通大豆生物学特性提高大豆产量的研究Ⅱ.中国扁茎大豆的生物学特性     

田佩占  袁全  孙永纯  王素云  李伟 《大豆科学》1998,(2)

中国扁茎大豆是个不稳定的分离群体。与普通大豆品种相比较,节数较多,节间较短,叶数较多,叶柄较短。群体中也有与美国扁茎大豆相似的顶端花序类型,开花结荚密集,但植株中下部仍然有大量花序。中国扁茎大豆可能是吉林２０号大豆品种的突变体。  相似文献   

早晚熟夏大豆品种花荚形成的比较     

张孟臣 《大豆科学》1998,17(3):236-241

通过对１７个有限、亚有限型夏大豆品种的研究结果,鼓粒前的花荚期长短是选择夏大豆品种生育期长短的重要因素。不同熟期类型品种间生殖器官发育规律存在差异,早熟品种较晚熟品种花、荚形成发育快、时间短、脱落也快。当早熟品种生育重心进入鼓粒前的中、大荚形成发育过程中（相当于Ｒ４阶段）时,晚熟品种的相对时期还处在花和幼小英  相似文献