牦牛SIRT3基因的克隆及其在各组织和睾丸不同发育阶段的表达     

王斌  佟连鑫  秦文昌  李键  殷实 《畜牧兽医学报》2019,50(12):2431-2439

  相似文献   

麦二叉蚜唾液蛋白基因Sg1655时空表达谱及其抑制小麦防御反应的功能          下载免费PDF全文

孙钰  张雨濛  刘晓蓓  陈巨莲  张勇  解海翠 《植物保护学报》2024,51(1):20-27

为进一步明确麦二叉蚜Schizaphis graminum唾液蛋白Sg1655在调控小麦防御反应中的作用,基于麦二叉蚜唾液腺转录组数据,利用PCR技术克隆获取Sg1655开放阅读框,利用生物信息学软件对其序列进行分析;通过实时荧光定量PCR（real-time quantitative PCR,RT-qPCR）技术检测Sg1655在麦二叉蚜不同组织及不同龄期中的表达;利用细菌III型分泌系统将Sg1655导入到小麦叶片内,检测其瞬时表达对小麦胼胝质积累的影响。结果表明,Sg1655开放阅读框全长375 bp,编码125个氨基酸残基,N端1~21位为信号肽序列,预测蛋白分子量为14.90 kD。麦二叉蚜Sg1655氨基酸序列与玉米蚜Rhopalosiphum maidis同源序列相致性最高,为89.52%;麦二叉蚜Sg1655与玉米蚜同源序列聚为一个分支,亲缘关系最近。Sg1655在麦二叉蚜各组织中均有表达,其中在唾液腺中表达量最高,Sg1655在麦二叉蚜各个龄期均有表达,且在不同龄期之间无显著差异。小麦叶片内Sg1655瞬时表达可显著抑制小麦胼胝质积累,表明该蛋白可作为潜在效应子参与抑制小麦防御反应。  相似文献   

Changes of BMP signaling pathway in mammary glands of mice with mammary hyperplasia     

SUN Li  GUO Dong-hui  LIU Fei  JIANG Ning  ZHENG Hong-xin 《园艺学报》2018,34(3):544-548

AIM: To determine the expression patterns of bone morphogenetic protein (BMP) signaling components in the mammary glands with hyperplasia (MGH) and the activation of this signaling pathway. METHODS: The female C57BL/C mice (8-weeks-old) were randomly divided into control group and hormone group with 15 mice in each group. The mice in control group were treated with PBS, while the mice in hormone group were intragastric administration with estradiol valerate (2.5 mg/kg) for 25 d, followed by progesterone (4 mg/kg) peritoneal injection for 5 d. At the end of treatments, the mammary glands were collected to determine the morphological changes, the mRNA expression of BMP signaling components and the phosphorylation level of Smad1/5/9. RESULTS: In MGH, a part of BMP signaling pathway molecules were differentially expressed (P<0.05), including BMP ligands BMP2, 4, 5, 6, 7, 9, 13 and 14, BMP receptor BMPR1A, and antagonists Chrdl1 and Twsg, whereas the expression levels of some molecules, such as BMP3, BMP12, BMPR1B, BMPR2, Chrd, Chrdl2 and Noggin, were not altered. The phosphorylation levels of Smad1/5/9 was up-regulated in MGH (P<0.05). CONCLUSION: BMP signaling pathway is activated in MGH through the abnormal expression of its components. BMP signaling pathway may be a new target for MGH therapy.  相似文献   

竹农参与技术创新模式的构建     

石德金余建辉刘燕娜谢志忠 《勤云标准版测试》2008,(12)

以解决技术供求矛盾为前提.把政府各级部门、科研机构、竹农、竹加工企业之间存在一种"断层"关系"接续"起来,提出了"接续"方法,即竹农参与技术创新模式的实施和运用.在本文中,提出了竹农参与技术创新模式的基本要求、参与效果的指标、参与模式的基本思路、基本运行机制和主要特色.  相似文献   

鄱阳湖湿地灰化苔草群落物种多度分布格局沿水分梯度的变化     

刘扬  施建敏  边子星  邓绍勇  裘利洪  张微微  缪伸义 《草业科学》2016,33(1):19-26

为揭示鄱阳湖湿地苔草群落的构建机制,选择灰化苔草(Carex cinerascens)群落作为研究对象,通过设立3条样带调查不同水分梯度的群落物种多度,选用断棍模型(BSM)、生态位优先占领模型(NPM)、优势优先模型(DPM)、随机分配模型(RAM)和生态位重叠模型(ONM)5个生态位模型对群落种-多度关系进行拟合,结果表明,1)灰化苔草群落可按土壤水分划分为高湿度、中湿度和低湿度3组,分别对应从湖边、湿地中间和湿地边缘的梯度分布;2)从高到低的水分梯度上,灰化苔草群落的物种数先增加后降低,优势种多度逐渐增加,物种多度分布曲线由相对平缓变为陡峭;3)高湿度灰化苔草群落物种多度分布格局的最佳拟合模型为BSM,中、低湿度的理想模型转变为NPM。研究认为,水分条件的变化是灰化苔草群落物种多度分布格局改变的主因,随着水分梯度降低,群落构建的生态学过程由随机生态位变为生态位优先占领。研究为鄱阳湖湿地多样性保育和生态功能管理提供了理论参考。  相似文献   

Effect of support on the growth of a woody vine,Wisteria floribunda DC. (Leguminosae)     

Atsushi Sakai  Wajirou Suzuki 《Journal of Forest Research》1999,4(2):183-186

Seedling ofWisteria floribunda, which belongs to the twiner type of climbing plants, were planted in nursery and the effect of support on the growth of the seedlings was tested. The seedlings were grown with 240 cm-height and 120 cm-height supports (the culms of a dwarf bamboo), and without support. Positive effect of the supports and its length were observed for stem length ofW. floribunda at the end of the growing season. The positive effect of support was also observed for total dry weight. Total dry weight with 240 cm-height support reached more than the double of the seedlings without support. These effects of the support were attributed to die-back behavior of the twining stem, which detached from the support or did not encounter the support. Diameter of the stems without support showed a rapid decrease toward the stem tip, while that with support were relatively constant when they were twining around the support. The existence of the support also affected the dry matter allocation in this species; supported seedlings allocated larger proportion of biomass to current-year stems than unsupported seedlings, although the allocation to the roots was smaller in the supported treatments. Support availability may be critical for the establishment and further growth of the seedlings ofW. floribunda which regenerate at the forest edge and canopy gaps. A part of this report was presented at the 107th Annual Meeting of the Japanese Forestry Society, at Tsukuba, April, 1996.  相似文献   

格氏栲种群生态学研究Ⅶ.格氏栲种群分布格局的强度与纹理分析     

刘金福 《中南林业科技大学学报(自然科学版)》1999,(1)

前人都是采用样方方法对格氏栲种群数量的空间格局进行测定,而格局分布有可能受样方大小的影响,且分析过程中没有涉及聚块间密度差的问题,因而无法掌握种群的聚块大小差别及聚块内个体间的离散程度．本研究采用无样方距离法,测定不同生境的格氏栲种群空间格局,分析格氏栲种群格局的强度和纹理．强度以聚块和间隙的密度差来定义,纹理则是体现聚块内个体间的离散程度与诸聚块间的分离程度．测定结果表明,格氏栲种群格局强度从高到低排列次序为：木姜子＞蚊母树＞冬青＞茜草树＞格氏栲＞米槠＞虎皮楠＞木荷;格局纹理从粗到细的顺序是：木荷＝木姜子＞茜草树＞格氏栲＝冬青＞米槠＞蚊母树＞虎皮楠．这一测定结果与作者采用聚集度指标测定相同样地格氏栲种群空间格局的结果基本相符．因此,格氏栲空间格局类型及分布与格氏栲生物学特性及生境的关系密切  相似文献   

基于改进方向纹理谱特征的图像检索     

孙君顶 《水土保持学报》2012,(4):812-818

在定义图像局部邻域纹理方向特性的基础上,提出了一种新的方向纹理谱描述符。该描述符针对局部邻域内中心像素与其相对的邻域像素,既充分考虑了它们间的灰度变化关系,又考虑了它们间灰度差异的变化关系,从而更有效地描述了局部纹理特征。为证明新描述符的分辨能力,采用4种不同图像库进行图像检索对比实验,结果表明,本文的新纹理谱描述符取得了最好的检索效果。  相似文献   

黄土高原土地资源及合理利用原则的探讨     

巨仁  宋桂琴  李锐  杨勤科 《水土保持研究》1992,(2):1-12

依据多年来,特别是近几年来（国家“六五”、“七五”期间）的研究积累,从黄河中游水系整治的完整性和区域经济开发的完整性出发,系统论述了本地区发展旱农的地理景观与人文历史经济背景;提出了农业土地资源分类系统及不同类型分片的土地类型构成数据;提出了若干土地合理利用的共性原则:生态效益与经济效益相统一原则;综合配置上的镶嵌性原则;增强第一性生产者原则;土地利用优化设计上的总体大于部分之和原则以及旱农用地的保护与培肥原则。  相似文献   

氨维营养强化剂的研究和利用     

台建祥  付勤 《作物学报》1990,16(2):153-160

利用优质蛋白源—大豆饼粕强化谷物,通过粮豆蛋白质互补、生物强化和生物技术等方法,将营养价值较低的原料转化为营养丰富的组合食品—氨维营养强化剂。它氨基酸含量较高、组成比例较协调(接近 FAO 推荐值)。它含有包括人体必需的氨基酸共18种,多种维生素和矿质元素。经国内外有关专家品尝、鉴定,它是优质饮料和食品的强营养  相似文献