Functional polymorphism among members of abscisic acid receptor family(ZmPYL) in maize          下载免费PDF全文

LU Feng-zhong  YU Hao-qiang  LI Si  LI Wan-chen  ZHANG Zhi-yong  FU Feng-ling 《农业科学学报》2020,19(9):2165-2176

Pyrabactin resistance 1-like proteins(PYLs) are direct receptors of abscisic acid(ABA). For the redundant and polymorphic functions, some members of the PYL family interact with components of other signaling pathways. Here, 253 positive colonies from a maize cDNA library were screened as interacting proteins with the members of ZmPYL family. After sequencing and function annotation, 17 of 28 interaction combinations were verified by yeast two-hybrid(Y2 H). The germination potential, taproot length and proline content of a quartet mutant of Arabidopsis PYL genes were significantly deceased comparing to the wild type(WT) under alkaline stress(pH 8.5) and 100 μmol L–1 methyl jasmonate(MeJA) induction. The malondialdehyde(MDA) content was significantly increased. After germinating in darkness, the characteristics of dark morphogenesis of the quartet mutant seedlings were more obvious than those of the WT. The differential expression of the related genes of photomorphogenesis in the mutant was much more than that in the WT. Three light and two JA responsive cis-affecting elements were identified during the promoter sequences of the AtPYL1 and AtPYL2 genes. These results suggested that functional polymorphism has evolved among the members of ZmPYL family. In response to developmental and environmental stimuli, they not only function as direct ABA receptors but also interact with components of other signaling pathways mediated JA, brassinosteroid(BR), auxin, etc., and even directly regulate downstream stress-related proteins. These signaling pathways can interact at various crosstalk points and different levels of gene expression within a sophisticated network.  相似文献   

Effect of three insect-resistant maizes expressing Cry1Ie,Cry1Ab/Cry2Aj and Cry1Ab on the growth and development of armyworm Mythimna separata(Walker)          下载免费PDF全文

SU Hong-hua  JIANG Tao  SUN Yu  GU Hui-jie  WU Jiao-jiao  YANG Yi-zhong 《农业科学学报》2020,19(7):1842-1849

Three transgenic maize events(IE09 S034, Shuangkang 12–5 and C0030.3.5) produced Cry1 Ie, Cry1 Ab/Cry2 Aj and G10-EPSPS, Cry1 Ab and EPSPS, respectively, all of which target the Asian corn borer. The oriental armyworm Mythimna separata(Walker) is the secondary target. In this study, the effects of the three Bt maizes on the development and survival of armyworm were studied. The results showed that IE09 S034 had insecticidal activity against 1 st instar larvae, and the survival rate of armyworm fed with Bt maize for 10 days was 46.2%, significantly lower than that of the control. The larvae at 3 rd–6 th instar were more tolerant of the Bt toxin than the early instar larvae. However, Shuangkang 12-5 had good insecticidal activity against 1 st–5 th instar larvae. The mortality was nearly 100% when the larvae were fed with Shuangkang 12-5 before 3 rd instar, and the toxin had quick-acting efficacy. This event significantly inhibited the development of armyworm; that is, the larval duration of the 3 rd and 4 th instar larvae fed with Shuangkang 12-5 was prolonged by 4.5 and 3.0 days, respectively. The pupal weight and egg number were also significantly lower than those of the control. For C0030.3.5, it could control 1 st–5 th instar larvae effectively. The mortality rates were all over 50% if 1 st–3 rd larvae were fed with this event. The pupal weight of 4 th–6 th instar larvae fed with Bt maize were only 53.9, 56.8 and 54.6%, respectively, compared to that of the control. The number of eggs laid was significantly less than the control. The results indicate that all three transgenic maize events exhibit the potential to provide effective control of early instar larvae of armyworm, which can be commercialized in future to control lepidoptera pests such as Asian corn borer and armyworm.  相似文献   

Effects of post-silking water deficit on the leaf photosynthesis and senescence of waxy maize          下载免费PDF全文

YE Yu-xiu  WEN Zhang-rong  YANG Huan  LU Wei-ping  LU Da-lei 《农业科学学报》2020,19(9):2216-2228

Waxy maize is widely cultivated under rainfed conditions and frequently suffers water shortage during the late growth stage. In this study, a pot trial was conducted to examine the effects of post-silking drought on leaf photosynthesis and senescence and its influence on grain yield. Two waxy maize hybrids, Suyunuo 5(SYN5) and Yunuo 7(YN7), were grown under the control and drought(soil moisture content was 70–80% and 50–60%, respectively) conditions after silking in 2016 and 2017. The decrease in yield was 11.1 and 15.4% for YN7 and SYN5, respectively, owing to the decreased grain weight and number. Post-silking dry matter accumulation was reduced by 27.2% in YN7 and 26.3% in SYN5. The contribution rate of pre-silking photoassimilates transferred to grain yield was increased by 15.6% in YN7 and 10.2% in SYN5, respectively. Post-silking drought increased the malondialdehyde content, but decreased the contents of water, soluble protein, chlorophyll, and carotenoid in the leaves. The weakened activities of enzymes involved in photosynthesis(ribulose-1,5-bisphosphate carboxylase and phosphoenolpyruvate carboxylase) and antioxidant system(catalase, superoxide dismutase and peroxidase) reduced the photosynthetic rate(P_n) and accelerated leaf senescence. The correlation results indicated that reduced Pn and catalase activity and increased malondialdehyde content under drought conditions induced the decrease of post-silking photoassimilates deposition, ultimately resulted in the grain yield loss.  相似文献   

江汉平原植棉区春玉米与短季棉套作的种植表现     

陈舫  李水清  雷昌云 《棉花科学》2020,42(1):54-56

为探索适宜江汉平原植棉区监利县旱地作物高产高效种植模式,开展了春玉米与短季棉套种模式示范试验。结果表明:春玉米与短季棉套种模式达到了玉米和棉花双丰收的高产效果,经济收益为33000元/hm^2左右,明显高于玉米和棉花的单作收益,是旱地作物结构调整的较好模式。  相似文献   

春甘薯—秋白菜复种栽培技术规程     

宋聚红  刘玉芹  王海山 《蔬菜》2020,(4):39-41

为了解决石家庄地区春甘薯—秋白菜生产中品种不配套、栽培技术不规范、病虫害较为严重等问题,充分发挥甘薯节水抗旱、营养均衡、祛病保健,白菜营养丰富、品质柔嫩、口味颇佳的独特优势,特编写了《春甘薯—秋白菜复种栽培技术规程》,详细规范了春甘薯—秋白菜复种栽培的基础条件、茬口安排、品种选择以及春甘薯栽培、秋白菜复种的技术要点。确保了每667 m^2春甘薯产量在2000 kg以上,大白菜产量在4000 kg以上,每667 m^2种植效益比传统模式增加1000元。为种植春甘薯、秋白菜的菜农和蔬菜生产企业提供了技术支撑。  相似文献   

扇吸式太阳能杀虫灯在鲜食玉米田的应用效果及对节肢动物生物多样性的影响   总被引：1，自引：0，他引：1  

韩海亮  章金明  刘敏  赵福成  王桂跃  吕要斌 《植物保护学报》2020,47(6):1234-1243

为明确扇吸式杀虫灯在鲜食玉米田间的应用效果及对节肢动物生物多样性的影响,采用抽样鉴定方法调查扇吸式杀虫灯的诱虫谱,通过田间试验调查其对主要害虫亚洲玉米螟Ostrinia furnacalis的控制范围和防控效果,并采用黄板、糖醋酒液和陷阱法取样调查其对玉米田间节肢动物生物多样性的影响。结果表明,扇吸式杀虫灯在鲜食玉米田可诱集9目40科的昆虫,对亚洲玉米螟的有效控制距离在60 m左右,单灯控制面积约1.1 hm²。在大喇叭口期,灯光防控处理对亚洲玉米螟的株防效为25.83%,显著低于化学防控处理的76.74%;在乳熟期,化学防控处理的虫孔减退率为75.29%,显著高于灯光防控处理的21.18%,灯光防控处理、化学防控处理和对照的茎秆活虫数、虫孔隧道长度和雌穗防效差异均不显著。距灯80 m范围内不同距离的主要害虫与天敌种群数量基本一致,玉米不同生育期节肢动物群落多样性指数、均匀性指数、优势集中性指数和相似系数变化较大,但在同一生育期不同距离间均无显著差异,说明田间节肢动物群落主要受玉米生育期的影响,扇吸式杀虫灯对田间主要害虫和天敌种群无显著影响。表明扇吸式杀虫灯对亚洲玉米螟的防治效果有限,对田间节肢动物多样性无显著影响,可作为鲜食玉米田防治亚洲玉米螟的辅助手段,但无法替代化学防治。  相似文献   

1971—2016年河南省夏玉米生长季极端干旱时空特征   总被引：2，自引：0，他引：2  

薛昌颖  张永涛  刘伟昌 《干旱地区农业研究》2020,38(2):258-266

选用地表湿润指数,利用1971—2016年气象数据对河南省夏季极端干旱发生的时空特征进行了分析。结果表明：近46 a来,河南省夏季极端干旱发生频数在年均0.08～2.15月之间,总体上呈现微弱的下降趋势。6月和9月极端干旱发生次数高于7月和8月。1970s发生频数最多,2000s最少,2010s呈现回升的趋势。6月和9月极端干旱发生站次百分比明显高于7月和8月,且6、7月和9月的发生站次百分比在2010s也呈现回升趋势。各年代平均发生频数均以豫南地区最高,近46 a来极端干旱总次数呈现由南向北逐渐递减的分布特征,但6月份发生总次数高值区则主要分布在豫中和豫西地区。全省范围内极端干旱发生存在明显的4~8 a周期变化,2010s极端干旱发生频数和站次百分比均呈回升趋势,应引起夏玉米生产上的关注和重视。  相似文献   

河套灌区盐渍化土壤玉米水氮耦合效应   总被引：1，自引：0，他引：1  

闫建文  史海滨  李仙岳  田德龙  李祯 《干旱地区农业研究》2020,38(4):103-110

为了探求适用于盐渍化地区的节水、施肥优化模式,采用大田试验,以玉米为研究对象,选取了轻度和中度两种盐分土壤,设置了10种不同水、氮处理,建立不同盐分土壤玉米产量与灌水量及施氮量之间的回归模型并对其进行了分析,研究不同盐分土壤水、氮用量对玉米产量的影响。结果表明：在轻度和中度盐分土壤条件下,灌水和施氮对玉米均有增产效应,水、氮交互作用为正效应,水分的作用大于施氮的作用。通过边际效应分析可知,轻度和中度盐分土壤施氮肥的增产速率没有明显差异,轻度盐分土壤灌水的增产效率明显高于中度盐分土壤。轻度和中度盐分土壤玉米最高产量分别为13 581 kg·hm^-2和11 115 kg·hm^-2,对应的水、氮配比均为灌水编码为0.77（全生育期灌水量2 250 m³·hm^-2）,施氮编码为0.69（总施氮量225 kg·hm^-2）。通过模型寻优,得到轻度和中度盐分土壤种植玉米的最佳水、氮配比方案均为全生育期灌水量为1 900.95~2 389.08m³·hm^-2,总施氮量为174.04~240.7 kg·hm^-2。优化方案的水、氮用量分别比当地灌水量（2 925 m³·hm^-2）节水18.2%~35%, 施氮量（325 kg·hm^-2）节肥26.0%~46.4%。优化范围包含了轻度和中度盐分的最高产量水、氮用量,产量与当地产量基本一致,符合当地灌水施肥要求。但从维持目前玉米产量和长期盐碱地改良的角度看,建议中度盐化土壤应选取水、氮优化范围中中等偏上灌水量和中等偏下的施肥量,以便于从根本上降低土壤盐分背景值,便于长期产量的提高;轻度盐分土壤选取优化范围适中的水、氮用量。  相似文献   

旱作覆膜玉米生长和水分利用对气候变化的响应     

董文俊  刘健峰  丁奠元  陈紫薇  李悦  王乃江  冯浩 《干旱地区农业研究》2020,38(1):1-12

基于3 a连续冬小麦-夏玉米覆膜轮作试验校准和验证AquaCrop模型的适用性,模拟研究了气候变化对夏玉米生长、产量和水分利用的影响,分析了覆膜措施对气候变化的应对效果。结果表明:关中地区(以武功、宝鸡和西安地区为例)年平均温度呈逐年递增趋势,温度增加幅度由高到低依次为宝鸡、西安和武功,增温速率分别为0.20、0.12℃·10a~(-1)和0.09℃·10a~(-1);降雨量呈逐年递减趋势,减少幅度大小依次为西安、宝鸡和武功,减小量分别为3.59、3.23 mm·10a~(-1)和2.64 mm·10a~(-1)。AquaCrop模型在关中地区表现出了良好的适用性,可以较好地模拟连续覆膜条件下作物的产量指标、水分利用和生长的动态变化,冠层覆盖度模拟值和实测值之间的均方根误差(RMSE)介于1.1%～15.3%,生物量模拟值和实测值之间的RMSE介于0.626～2.540 t·hm~(-2),土壤贮水量模拟值和实测值之间的RMSE介于12.6～47.4 mm。模拟研究表明,上世纪60年代以来,随着气温不断升高,武功、宝鸡和西安地区的夏玉米生育期均呈逐年缩短趋势,特别是1980s以来,减少幅度达2.76、4.82 d·10a~(-1)和5.94 d·10a~(-1);在不同的降水年型下,覆膜处理产量均高于裸地处理,且其变异系数较小;在干旱气候条件下,覆膜处理依然可以获得一定的籽粒产量,与裸地处理相比,覆膜处理表现出了较好的稳产效应。同时,覆膜处理有效减少了玉米苗期土壤表层蒸发(平均减少7.6 mm),从而在土壤中保蓄更多的降雨;覆膜处理虽然增加了土壤耗水量,但其通过保蓄土壤水分,稳定作物产量,有效提高了玉米的水分利用效率。因此,关中地区旱作覆膜可以有效适应当地气候变化,在一定程度上应对干旱气候,具有较好的增产稳产效应。  相似文献   

干旱胁迫下玉米自交系抗旱性评价及筛选     

周玉乾  杨彦忠  周文期  连晓荣  张彦军  王兴荣  寇思荣  何海军  刘忠祥  王晓娟 《干旱地区农业研究》2020,38(5):211-217

以39份玉米自交系为材料,2017—2018年在甘肃省张掖市设置田间自然抗旱鉴定试验,筛选抗旱鉴定指标,综合评价玉米自交系的抗旱性。结果表明：在干旱胁迫下,39份玉米自交系的粉丝间隔时间延长了0~3 d,株高、穗位高、穗长、穗粗、穗干重、籽粒干重、出籽率及百粒重均降低,其中,穗干重、籽粒干重和出籽率3个指标分别为正常灌水处理的81.5%、79%和88.5%。穗干重、籽粒干重和出籽率与平均抗旱系数呈显著正相关,可作为玉米自交系抗旱鉴定的主要指标。利用加权抗旱系数法综合评价筛选出强抗旱玉米自交系10份,其中自交系E28、DH351、陇1222、浚92-8和黄早四,抗旱性达到极强。本研究所筛选到的抗旱自交系可在选育抗旱新品种中加强利用。  相似文献