β-聚糖酶的诱导合成(Ⅰ)--碳源、氮源及碳氮比的影响     

李坤平  潘天玲  吕晓霞  黄克瀛 《中南林业科技大学学报(自然科学版)》2004,24(2):70-72

以绿色木霉Trichoderma viride LH-0ll为产酶菌株,利用自制复合碳源,就碳源浓度、氮源种类及碳氮比等因素对β-聚糖酶的诱导合成的影响进行了研究.结果表明:以15 g/L预处理玉米芯Ⅰ为碳源,利用复合氮源((NNH4)2SO4 尿素 蛋白胨).控制C/N为6.4,调节初始pH值4.8,温度为28～30℃,150 r/min摇瓶培养72 h,纤维素酶活力和木聚糖酶活力分别达到了3.34 U/mL和13.2 U/mL.  相似文献   

木聚糖酶酶活测定条件的优化   总被引：1，自引：0，他引：1  

徐君飞  顾佳佳  刘正初  张居作 《农产品加工．学刊》2007,(7):7-10

采用DNS法,探讨了不同测定条件对1株欧文氏杆菌变异菌株CXJZ11-01所产木聚糖酶活性的影响及其产酶规律。研究结果表明,该木聚糖酶活性测定的最佳条件为:采用0.1mol/L柠檬酸—柠檬酸钠缓冲体系,pH值为5.2￣5.8,木聚糖质量分数为0.8%,温度为60℃,DNS用量为2.0￣2.5mL。在适宜测定下,该菌株发酵液酶活最高达344U/mL。  相似文献   

嗜热拟青霉固体发酵产木聚糖酶的纯化和应用研究   总被引：1，自引：0，他引：1  

杨绍青  王岚  江正强 《农产品加工．学刊》2006,(8):75-78

利用一株拟青霉新种嗜热拟青霉J18,以天然农业废弃物小麦秸杆为碳源固体发酵产木聚糖酶,优化发酵条件后,产木聚糖酶水平达到16500U/g干基碳源。粗酶液经硫酸铵沉淀、凝胶过滤层析和离子交换层析3个步骤纯化,得到了电泳级纯酶。研究了该酶对馒头品质的影响,发现在面团中添加木聚糖酶能够明显改善面团的操作性能,提高馒头的比容,改善馒头的组织结构,延缓馒头的老化。  相似文献   

Effects of enzyme addition on the nutritive value of broiler diets containing hulled or dehulled Chinese double-low rapeseed meals     

Fang ZF  Liu ZL  Dai JJ  Qian HY  Qi ZL  Ma LB  Peng J 《Journal of animal physiology and animal nutrition》2009,93(4):467-476

An in vitro and a feeding trial was conducted to investigate the effect of fibre-degrading enzymes A (xylanase + β-glucanase), B (xylanase) and C (xylanase + cellulase) on the nutritive value of broiler diets containing either hulled (22.5% and 23.5% for 4–21 days and 22–42 days of age, respectively) or dehulled (20% and 21.5%) Chinese double-low rapeseed meals (DLRM). Overall, in vitro digestibility of dry matter (DM) or neutral digestibility fibre (NDF) did not differ (p > 0.05) because of meal types; both crude protein (CP) and NDF digestibility was improved (p < 0.05) because of addition of enzymes B or C either to hulled or dehulled DLRM diets. Birds fed dehulled DLRM diets had a higher (p < 0.05) growth rate, feed efficiency and lower (p < 0.05) feed intake than those fed hulled DLRM diets during the overall phase. Enzyme C addition to dehulled DLRM diets resulted in improved (p < 0.05) growth rate and feed efficiency during 4–21 days of age. Enzymes A and B addition elicited a positive response in feed intake and weight gain (p < 0.05), respectively, but did not affect (p > 0.05) feed efficiency. It would appear that the nutritive value of broiler diets containing Chinese DLRM could be improved by appropriate xylanase-based enzymes. Responses of broilers to fibre-degrading enzymes could be highlighted by hull removal of fed DLRM.  相似文献   

Effects of beta-glucanase and xylanase supplementation on gastrointestinal digestive enzyme activities of weaned piglets fed a barley-based diet     

Fan CL  Han XY  Xu ZR  Wang LJ  Shi LR 《Journal of animal physiology and animal nutrition》2009,93(2):271-276

The effects of supplementing a barley-based diet for weaned piglets withexogenous beta-glucanase and xylanase on gastrointestinal digestiveenzyme activities were investigated. Thirty-six cross-bred weaned pigletswere randomly assigned to two groups with three pens based on sexand mass. Each group was fed on the diet based on barley with or withoutadded beta-glucanase and xylanase (0.15%) for a 4-week period. Theresults showed that enzyme supplementation improved growth performanceof piglets significantly (p < 0.05), but had no effect (p = 0.091)on average daily feed intake. The results also showed that supplementationof beta-glucanase and xylanase had no effect on pepsin activity in gastriccontents but slightly decreased (p = 0.092) the pepsin activity ingastric mucosa. Meanwhile, no effect of enzyme supplementation ontrypsin activity in duodenal contents was observed. However, the activitiesof amylase and lipase in duodenal contents were significantly(p < 0.05) decreased, whereas the activities of maltase, sucrase andgamma-glutamyl transpeptidase (gamma-GT) in jejunal and ileal mucosa wereenhanced significantly (p < 0.05). The improvement of disaccharidaseand gamma-GT activity may be attributed to the positive impacts of exogenousenzymes on digestion and absorption of the nutrients. In conclusion,the current results indicated that supplementation with enzymes in barley-based diets could improve the growth performance of piglets,decrease the activities of amylase and lipase in duodenal contents andincrease the activities of disaccharidase and gamma-GT in jejunal and ilealmucosa.  相似文献   

两种粗酶制剂对肉鸡生产性能的影响   总被引：1，自引：0，他引：1  

朱元招  刘亚力 《中国家禽》1999,21(7):7-9

选用６００只１日龄石岐杂肉鸡，随机分成对照和试验两组ＣＥ１与ＣＥ２，每组两重复。饲以玉米基础日粮，ＣＥ１组添加０１％由木聚糖酶、β－葡聚糖酶、果胶酶与蛋白酶组成的酶制剂Ⅰ，ＣＥ２组添加０１％含淀粉酶、蛋白酶与纤维素酶的酶制剂Ⅱ，试验期６０ｄ测定生产性能及有关消化器官参数。结果显示，ＣＥ１和ＣＥ２组鸡全期平均增重较对照组鸡分别提高７０９％和５５９％，料重比分别下降６５１％和５２１％，相对生长的增加持续到中期；三组的小肠相对长、胰腺相对重和死亡率无显著差异。  相似文献   

木聚糖酶对不同木聚糖含量的仔猪饲粮养分消化率的影响   总被引：1，自引：0，他引：1  

黄金秀  陈代文  张克英 《中国畜牧杂志》2008,44(7):21-24

试验设计3个消化试验分别研究在3个饲粮木聚糖水平下添加不同剂量的木聚糖酶对养分表观消化率的影响。饲粮木聚糖水平分别为5.33%、6.47%和8.44%。每个消化试验(即每个木聚糖水平下)设5个处理,分别饲喂不添加酶的基础饲粮和添加4个木聚糖酶水平的饲粮。随着饲粮木聚糖含量的提高,木聚糖酶的添加量相应增加。结果表明:当饲粮木聚糖含量低于6.47%时,添加木聚糖酶对养分消化率没有改进作用,而当木聚糖含量高达8.44%时,木聚糖酶的添加对养分消化率有一定的改进效果。与不加酶组比,加酶组粗蛋白、有机物、钙、磷、酸性洗涤纤维和能量的平均消化率分别提高了2.92%、1.05%、5.80%、5.15%、2.36%和0.84%。  相似文献   

小麦日粮非淀粉多糖和木聚糖酶对肉仔鸡肠道微生物区系的影响   总被引：13，自引：0，他引：13  

王金全  蔡辉益  周岩华  刘国华  张姝  印遇龙 《畜牧兽医学报》2005,36(10):1014-1020

采用传统微生物培养和PCR-DGGE 2种方法，针对肠道内几种微生物的数量和优势菌的多样性进行研究。将90只AA肉仔鸡随机分成3组，分别饲喂玉米-豆粕型，小麦-豆粕型和小麦-豆粕型添加木聚糖酶3种日粮。结果表明，小麦非淀粉多糖有增加肠道内几种微生物数量的趋势，其中显著增加了回肠和盲肠厌氧菌总数以及盲肠粪链球菌数量（P＜0．05），而PCR-DGGE结果表明，小麦NSP改变了肠道内微生物的种群结构，降低了种群的数量（P＜0．05）。添加木聚糖酶对回肠和盲肠的微生物数量以及种群数量有影响，但未表现出显著性差异（P＞0．05）。说明小麦非淀粉多糖作为微生物发酵的底物对肠道厌氧微生物产生了增殖效应，但却抑制了其他微生物的生长，导致肠道内微生物结构发生改变。  相似文献   

耐热木聚糖酶水解法制备木寡糖的研究     

朱孝霖  Li Huan  韦萍 《饲料工业》2008,29(12):39-42

为探讨耐热木聚糖酶水解自制甘蔗渣木聚糖制备木寡糖的工艺条件和分析水解液的寡糖成分,试验以产耐热木聚糖酶的基因工程菌1020为原料,水解自制的甘蔗渣木聚糖,分别研究水解过程中酶的用量、木聚糖的浓度及酶解温度对酶解反应的影响,并采用高压液相色谱和质谱联用技术对水解液成分进行分析。试验结果表明,2%的木聚糖浓度,水解温度95℃,酶液用量100U/g,酶解3h后DP值可降至2.94,满足生产木寡糖对聚合度的要求;并且水解液的成分主要为木糖、木二糖和木三糖,没能检测到木四糖。耐热木聚糖酶在高温下酶解木聚糖对酶法制备木寡糖具有重要的应用价值。  相似文献   

应用琼脂扩散法测定饲料中木聚糖酶含量     

黄辉  李富伟  汤海鸥  汪勇 《中国饲料》2008,(22)

本实验研究了应用琼脂扩散法测定饲料中所舍木聚糖酶的活性.结果表明,该方法标准曲线制作线性良好;应用淀粉稀释木聚糖酶进行验证性实验,检测酶活测定值和实际理论值之间差异不显著(P>0.05),说明该方法准确率很高;应用饲料稀释木聚糖酶进行验证性实验,检测酶活值低于理论值,但这并不影响该方法对饲料中酶活的判定.综合整个实验结果表明,此方法可以很好的检测饲料中含有的微量木聚糖酶.  相似文献