排序方式: 共有12条查询结果,搜索用时 187 毫秒
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自制1.5%绵羊红细胞诊断液并用于检测牛肺疫抗体,结果如下:(一)三批标准阳性血清(批号为8601、8301、8001)凝集价均在160倍以上,标准阴性血清(批号为8601)凝集度仅为10倍。15份被检血清IHA的阳性检出率(8/15)高于补体结合反应(CF,6/15)且凝集价均在40倍以上。(二)用牛肝片吸虫病、布氏杆菌病和附红细胞体病阳性血清作类症鉴别诊断,凝集度仅为10倍。(三)把牛肺疫CF8502抗原作倍比稀释,加2个凝集单位牛肺疫阳性血清8601和8301进行间接血凝抑制试验,分别被32倍和16倍稀释的抗原所抑制,本项试验结果表明IHA用于检测牛肺疫,具有检出率高、特异性强、设备要求简单、容易操作等特点,适于在现地推广应用。 相似文献
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牛肺疫是比较常见的一种牛接触性传染病症,严重危害着牛机体。随着近些年国内畜牧业的迅猛发展,牛养殖场规模与数量随之不断扩大,我国养殖业呈现繁荣发展局面。然而,牛养殖期间也会突发各种疫病,带给畜牧生产巨大的经济损失。所以,有效的牛肺疫诊断防控措施对我国养殖业的发展极具重要意义。 相似文献
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为获得纯净山羊传染性胸膜肺炎支原体生产用菌种,将山羊传染性胸膜肺炎C87001株冻干肺组织(1980年),通过采用绵羊肺炎支原体代谢抑制试验结合固体培养基挑取单个克隆菌落方法,进行克隆纯化。结果表明,制苗用C87001株冻干肺组织(1980年)纯化后克隆株1~15代菌种均纯净、每代活菌滴度均≥10^9.0CCU/mL,经菌落形态和PCR鉴定,获得克隆纯化的F5代山羊传染性胸膜肺炎支原体生产用菌种。该菌种经本动物回归试验,4/4发病;按规程制备灭活疫苗免疫山羊,攻毒保护为4/4(100%),对照组3/3发病死亡。 相似文献
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Ozdemir U Loria GR Godinho KS Samson R Rowan TG Churchward C Ayling RD Nicholas RA 《Tropical animal health and production》2006,38(7-8):533-540
The efficacy of danofloxacin (Advocin A180) was evaluated for the treatment of contagious caprine pleuropneumonia (CCPP) caused by Mycoplasma capricolum subsp. capripneumoniae. Ten healthy Angora goats, confirmed free of CCPP, were exposed to clinically affected animals from a natural outbreak in Thrace, Turkey. After 14 days exposure, 8 goats showed pyrexia ( > or = 41 degrees C). Shortly after, the Angora goats were divided randomly into two groups. Five of these were injected with danofloxacin (6 mg/kg subcutaneously), which was repeated after 48 h; the five remaining animals received saline. Goats were monitored clinically and blood samples were collected for serology. Animals with severe disease were withdrawn from the trial. Goats completing the study were euthanized at day 42. Lung tissue and bronchial fluid were collected for mycoplasma isolation. All danofloxacin-treated goats showed resolution of clinical disease by the end of the trial. Two saline-treated goats failed to complete the study owing to CCPP. Danofloxacin-treated goats showed fewer lung lesions and had significantly lower combined clinical scores than saline controls (p < 0.001). Danofloxacin was found to be highly effective in the treatment of CCPP in goats. 相似文献
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选择母猪APPⅠ型补体结合(CF)抗体未达4倍的12头仔猪(A群)和CF抗体达32倍的12头仔猪(B群),分别测定其生后4~48日龄和2~46日龄的抗体价及脏器中的抗生素浓度,结果从A群仔猪病料中来分离出放线菌,但B群仔猪2日龄就从气管分离出了放线菌,经凝集反应鉴定血清型为Ⅰ型,且对四不素类药物有较强的敏感性;其次是A群仔猪的抗体价全过程未达4倍,而B群在2日龄就高达32倍,10日龄后略显下降,但在结束日龄又有所回升,此外,根据高速色层分离法测定结果,试猪采食或肌注的体内四环素浓度在肝、肾、肺都较高,在气管较低,群间无明显差异。 相似文献