2014年中国不同地区对虾白斑综合征病毒ORF14/15和ORF23/24缺失区序列比较          下载免费PDF全文

孙新颖  刘庆慧  万晓媛  黄倢 《渔业科学进展》2016,37(4):140-146

对虾养殖面临诸多病害威胁,对虾白斑综合征病毒(White spot syndrome virus, WSSV)是养殖对虾主要病原之一,WSSV不同地理株的变异可能导致WSSV毒力的变化。为了解2014年中国大部分地区WSSV ORF14/15和ORF23/24的变异情况,本研究选择2014年1月–8月期间采集的48份WSSV阳性样本,用特异引物扩增ORF14/15和ORF23/24片段,连接于T载体,转化至Top10中,筛选阳性克隆,测序分析不同样本之间的缺失差异。结果显示,能够扩增ORF14/15和ORF23/24样品的比例分别为43.75%和33.33%。在ORF14/15扩增中,分别扩增出1260 bp、1270 bp、1892 bp和2662 bp片段,与TH-96-Ⅱ比对共有4种缺失情况,即缺失6540 bp、6530 bp、5908 bp和5138 bp。而在ORF23/24扩增中,分别扩增出1140 bp和1146 bp片段,与中国台湾株(TW)比对有两种缺失情况,即缺失12070 bp和12064 bp。研究结果表明,WSSV在中国大部分地区存在一定程度的变异,而不同毒株之间在ORF14/15可变区差异比较明显,在ORF23/24可变区差异不大,但均具有大片段缺失。  相似文献   

Control of western corn rootworm via RNAi traits in maize: lethal and sublethal effects of Sec23 dsRNA     

Ana M Vlez  Elane Fishilevich  Murugesan Rangasamy  Chitvan Khajuria  David G McCaskill  Adriano E Pereira  Premchand Gandra  Meghan LF Frey  Sarah E Worden  Shannon L Whitlock  Wendy Lo  Karl D Schnelle  Jamie R Lutz  Kenneth E Narva  Blair D Siegfried 《Pest management science》2020,76(4):1500-1512

  相似文献   

α-三联噻吩对斜纹夜蛾SL细胞线粒体膜电位及细胞周期的影响#br#     

王玉健  张志祥  徐汉虹  廖美德  张前锋 《中国农业科学》2010,43(8):1601-1607

【目的】研究α-三联噻吩（α-T）对斜纹夜蛾（Spodoptera litura）SL细胞线粒体膜电位及细胞周期的影响。【方法】通过Rhodamine123染色和流式细胞术（flow cytometry,FCM）研究α-T处理SL细胞24 h和48 h后细胞线粒体膜电位的变化,并通过PI染色和FCM,测定α-T处理SL细胞24 h和48 h后细胞周期各时相百分率的变化。【结果】光活化后的α-T处理SL细胞24 h后,0.0625 μg•mL-1浓度和0.1250 μg•mL-1浓度处理组细胞线粒体膜电位的变化幅度不大,而0.5000 μg•mL-1浓度处理组细胞线粒体膜电位明显升高,48 h后高浓度处理组（1.0000 μg•mL-1）导致线粒体膜电位明显去极化;处理24 h后,低浓度（0.0625 μg•mL-1）处理组细胞被阻滞于S期,而高于此浓度的光照处理组细胞被阻滞于G2/M期,48 h后,浓度≤0.1250 μg•mL-1的光照处理细胞被阻滞于G2/M期,而浓度＞0.1250 μg•mL-1的光照处理组细胞被阻滞于S期。【结论】SL细胞经α-T处理后,细胞处于一系列复杂的动态变化中。当细胞本身不能扭转ROS造成的氧化损伤时,细胞线粒体膜电位和细胞周期时相即出现较大幅度的变化,这种变化决定着细胞的受损程度和细胞的死亡途径。  相似文献   

攀西地区葛藤根瘤菌的RFLP遗传多样性     

谢徽  张小平  K.Lindstrom 《四川农业大学学报》2009,27(3)

利用16S rDNA PCR-RFLP和16-23S IGS PCR-RFLP技术对分离自四川攀枝花的43株葛藤根瘤菌进行了遗传多样性研究。供试菌株的16S rDNA用HaeⅢ、MspⅠ、HinfⅠ和TaqⅠ酶切后具有16种遗传图谱类型。16S-23S rDNA IGS PCR-RFLP分析结果表明,在53%的相似性水平上,供试菌株与参比菌株分为两个分支,在81%的相似水平上所有菌株分为13个群。  相似文献   

利用组成型表达载体pMG36e电转化乳酸乳球菌ML23的研究   总被引：3，自引：0，他引：3  

张玉兰  柯晓静  郭红敏  谷新晰  田洪涛  郭兴华  罗云波 《河北农业大学学报》2010,33(4)

为了探明乳酸菌电转化的最适条件,提高电转化效率,本试验以大肠杆菌-乳酸菌穿梭型质粒pMG36e为载体,以乳酸乳球菌ML23为受体,利用电转化方法研究了受体菌株的生长状态与细胞壁处理方式、质粒浓度、电场强度、复苏培养基组成与复苏培养时间、受体菌株的限制修饰作用对电转化效率的影响。结果表明:含2%甘氨酸和0.5 mol/L蔗糖的MRS培养基培养至对数生长中期的细胞,在电场强度11.0 kV/cm、电阻200Ω、电容25μF下电击转化,转化后细胞在SMRS培养基中培养2 h,获得了较高的电转化效率。采用受体菌株修饰的质粒进行电转化,转化效率又提高了15倍以上,达1.05×105CFU/μg DNA。  相似文献   

拜田净防除稻田杂草应用技术研究     

赵学平  王强  吴长兴  戴芬  吴俐勤  徐浩  张人君 《浙江农业学报》2000,12(6):345-348

试验结果表明,新型除草剂拜田净对移栽稻田和直播稻田杂草均具有良好的防除效果,以１００～１５０ｇａ．ｉ／ｈｍ＾２的用量在稗草１．５～２．５叶期施药,除草效果稳定在８９．６％以上,持效期为４０ｄ,与扫弗特和绕地奥相当,且优于二氯喹啉酸。拜田净的杀草谱包括稗草、千金子、异型莎草、陌上菜、鸭舌草,防效随剂量提高而增加,并且１．５叶期施药的除草效果比２．５叶期施药的好。拜田净以喷雾法和毒土法施用均较好的防效。直播稻田喷雾时要求田间土表湿润,药后１ｄ灌入薄水层;移栽稻田毒土撒施时要求田面保持水层３ｃｍ,药后保水５～７ｄ。同时,拜田净对水稻安全,但高剂量（２００～２５０ｇａ．ｉ／ｈｍ＾２）下对１．５叶期直播稻有一定的药害。  相似文献   

Role of reactive oxygen species in 23-hydroxybetulinic acid-induced apoptosis in LoVo cells     

CHENG Yan  YE Wen-cai  SHI Jun-min  ZHANG Kun  LV Yan-qing  YAO Xin-sheng  Hiroshi Kurihara 《园艺学报》2007,23(4):772-775

AIM: To investigate the changes of reactive oxygen species (ROS) in apoptosis of LoVo cells induced by 23-hydroxybetulinic acid.METHODS: LoVo cells were treated with 23-hydroxybetulinic acid. The apoptotic morphological change was observed under the light microscope. Intracellular ROS production and the rate of apoptosis were detected by flow cytometry.RESULTS: LoVo cells improved apoptotic morphological changes treated with 23-hydroxybetulinic acid for 48 h. At concentrations of 25, 50, 100, 200 μmol/L of 23-hydroxybetulinic acid, the apoptotic rates of LoVo cells were (7.17±2.31)%, (15.60±4.02)%, (32.47±5.25)% and (52.71±5.93)%, respectively. The results indicated a certain concentration-dependent relationship. 23-hydroxybetulinic acid caused an increase in the ROS production, and the ROS levels were 2.83±0.80, 5.97±1.72, 12.53±2.57 and 16.73±4.58. Compared with the control group (2.13±0.32), the increase in ROS production in LoVo cells at the concentration of 100, 200 μmol/L of 23-hydroxybetulinic acid treatment was significant (P<0.05). CONCLUSION: 23-hydroxybetulinic acid induces LoVo cell apoptosis. The production of ROS may play a crucial role in the process of the LoVo cell apoptosis.  相似文献   

Dietary phosphorus restriction affects bone metabolism,vitamin D metabolism and rumen fermentation traits in sheep     

Oriana M. Köhler  Walter Grünberg  Nadine Schnepel  Alexandra S. Muscher-Banse  Abbas Rajaeerad  Jürgen Hummel  Gerhard Breves  Mirja R. Wilkens 《Journal of animal physiology and animal nutrition》2021,105(1):35-50

Homeostasis of calcium (Ca) and phosphate (P_i) is maintained by a concerted interplay of absorption and reabsorption via the gastrointestinal tract and the kidney and by storage and mobilization from the bone regulated mainly by parathyroid hormone (PTH), 1,25-dihydroxycholecalciferol and calcitonin. The present study aimed at characterizing the effects of dietary P restriction on bone, vitamin D metabolism and rumen fermentation traits reflecting the endogenous P cycle maintaining the ruminal P supply for microbial metabolism. The experiments were done in eleven female, non-pregnant, non-lactating four- to nine-year-old Black Headed Mutton sheep allotted to two feeding groups: "P-restricted" (0.11% P/kg DM and 0.88% Ca/kg DM) and "Control" (0.38% P/kg DM and 0.88% Ca/kg DM). Dietary P restriction did not lead to hypophosphataemia, probably due to a compensation by bone mobilization, demonstrated by increased serum concentrations of a resorption marker and altered gene expression in bone tissue. In addition, the RNA expression of fibroblast growth factor 23, a bone-derived factor involved in the regulation of vitamin D metabolism, was significantly reduced with dietary P restriction. Furthermore, several genes related to vitamin D metabolism and plasma concentrations of 1,25-(OH)₂D were associated with serum concentrations of phosphate (P_i). In the parotid gland, the expression of the P_i transporter NaPi2b was negatively associated with serum P_i and positively with parathyroid PTH expression. Although P_i concentrations in saliva and the gastrointestinal tract were significantly reduced, we found no adverse effects of the P-restricted ration on the production of short chain fatty acids, but slight differences in the production of butyrate as well as its relationship to rumen P_i and ammonia concentrations that might indicate an impact on ruminal fermentation.  相似文献   

Identification of human cytomegalovirus tegument protein pUL23 interacting with human IGFBP4     

XIAO Xiao-ping  ZHOU Tian-hong  GONG Jun-yuan  YANG Dan-li  YUN Yue-ming  LI Yue-qin  ZOU Yi  ZHANG Xin  LI Hong-jian 《园艺学报》2010,26(12):2378-2382

AIM: pUL23 is a protein encoded by human cytomegalovirus, which inhibits self propagation of the virus. The study was to investigate the function of pUL23 protein encoded by human cytomegalovirus and reveal the mechanism which inhibits propagation of the virus itself.METHODS: We screened a host proteins interacting with pUL23 from human embryonic kidney cDNA library using the yeast two-hybrid assay. GST-pull-down and immunoprecipitation techniques were applied to study the physical interaction between them in vitro and in vivo, respectively.RESULTS: We confirmed the interaction between pUL23 and IGFBP4 by pull-down and immunoprecipitation technology.CONCLUSION: These results might provide important information to the function of pUL23 in regulating self-reproducting.  相似文献   

国审品种洛麦23种子繁育法及示范推广效果分析     

余四平  吴少辉  张少澜  田文仲  段国辉  温红霞  李建强  王军  赵德军 《安徽农业科学》2014,42(4):1040-1041

洛麦23通过建立穗行圃或穗系圃,与“三圃制”结合,形成了4个级别的种子生产程序：先建立穗行圃,再建立穗系圃;穗行圃和穗系圃,隔年交替进行;穗行圃混合收获种子或穗系圃收获种子,再加繁一代即为育种家种子;育种家种子繁殖原原种,原原种繁殖原种。原种即应用于大田生产。通过和种子企业、农场、种子生产专业村合作,采用“科研单位＋种子企业＋农场＋生产基地”联合体,建立健全良种繁育体系。至2013年,洛麦23在黄淮麦区南片得到了较大面积的推广利用,累计推广应用面积在100万hm2以上,创社会经济效益13．7亿元。  相似文献