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51.
The chemical compositions of volatile oil from fruiting body of Armillaria luteo-virens in Qinghai Province were firstly analyzed with GC-MS and its relevant compositions were detected by calculating chromatographic peak area with normalized method.21 peaks were separated and 13 compositions were identified which were mainly unsaturated fatty acids,taking 97.1% of the total volatile oil.  相似文献   
52.
云南昭通天麻共生蜜环菌优良菌株筛选   总被引:2,自引:1,他引:2  
研究了自云南昭通本地分离获得的4株天麻共生蜜环菌的生长特性,并与1株外地优良天麻共生蜜环菌京-234进行对比。结果显示:各菌株间存在明显差异,菌索平均生长速度依次为京-234〉SNA04〉SNA02〉SNA03〉SNA01 菌丝萌发速度、菌索分枝状况、荧光强度及暗培养18 d后菌索生物量等生长特性表现最好的为SNA04,其次为京-234,SNA01和SNA02较差。4株昭通天麻共生蜜环菌中,菌株SNA04性状最优,SNA03菌株次之。  相似文献   
53.
蜜环菌水溶性多糖的分离、纯化及组成分析   总被引:2,自引:0,他引:2  
目的从蜜环菌子实体中分离水溶性粗多糖,进一步纯化,得到组分均一的多糖AMP,并研究其组成和性质。方法粗多糖经反复冻融、酶法和Sevage法联合脱蛋白、乙醇分级、柱层析等方法分离纯化得级分AMP。经Sephadex G-100、DEAE-Sephadex A-25、HPLC和比旋光度法验证,AMP分子量及极性均一。结果AMP为均一组分,气相色谱分析多糖组成表明,AMP由Glc、Gal、Man和Fuc组成,其摩尔比为9.98∶3.57∶1.34∶1.00。结论AMP为中性杂多糖。  相似文献   
54.
The aim of the present experiment was to evaluate the effect of commercial Trichoderma and arbuscular mycorrhizal fungi (AMF)-based bio-fertilizers on nectarine root growth, nutrient acquisition and replanting disease. The experiment was performed from 2008 to 2012 in an A. mellea-infected nectarine orchard subjected to the following treatments: 1) untreated control; 2) AMF-biofertilizer applied at planting (120 kg ha?1) and every year in spring and autumn at the rate of 6 kg ha?1; 3) Trichoderma spp applied at planting (5 g plant?1) and every year (in April, May and September) at 2.5 kg ha?1. AMF bio-fertilizers decreased root diameter and increased root survivorship. Leaf phosphorus concentration increased in AMF bio-fertilizers plots, while no significant treatments effects were observed on other leaf nutrient concentration. Although biofertilizer application did not affect soil microbial population, at the end of the trial (2012) the application of Trichoderma alone increased the population of the fungus.  相似文献   
55.
黄绿蜜环菌菌丝体培养条件初探   总被引:1,自引:0,他引:1  
在黄绿蜜环菌的驯化培养试验中,筛选出几种较适宜的琼脂培养基、原种培养基和液体培养基。采用斜面培养试验初步取得了生长温度、pH值等基本数据。其生长温度范围10℃~35℃,适宜温度25℃~30℃;pH值范围(灭菌前)4~10,适宜pH值6.0~7.0。  相似文献   
56.
王广耀  程喆 《北方园艺》2011,(1):181-182
以蜜环菌菌丝体及其发酵液为主要原料,采用正交实验对稳定剂及饮料配方进行优化。结果表明:蜜环菌保健饮料的最佳配方为蜜环菌菌汁10%、蔗糖10%、柠檬酸0.3%,稳定剂CMC为0.15%。  相似文献   
57.
通过野外调查和室内试验,对豫南野生假蜜环菌(Armillariella tabescens)形态及生态特征进行了研究。结果表明:野生假蜜环菌在豫南分布普遍,每年6至7月份发生于茶树(Camellia sinensis)和板栗(Castanea mollissima)间作林内或其它阔叶林内。子实体棕褐色,多丛生,形态美,香味浓,具优良商品性状。菌体由菌盖和菌柄组成,无菌托和菌环。菌盖菌肉和菌柄菌肉均由菌丝组成,菌丝无色透明,具隔、具分枝,无锁状联合现象。菌盖表面鳞片由黄棕色菌丝构成,菌丝细胞壁有内凸增厚现象。菌褶延生,子实层由幼担子、担子及担孢子组成。成熟担子顶端产生4粒担孢子。在不同培养基上,菌落及菌丝形态有明显差异。在PSA综合培养基上不产生菌索,暗处可产生较强荧光,并形成菌皮。菌皮由表面粗糙的菌丝和泡囊组成。  相似文献   
58.
Naturally regenerated paper birch (Betula papyrifera Marsh.) is commonly removed from juvenile interior Douglas-fir (Pseudotsuga menziesii var. glauca [Beissn.] Franco) plantations in southern interior British Columbia, Canada, to increase conifer productivity and create a free-growing stand; however, this practice is expensive and contentious because of possible negative ecological impacts. One solution is to retain an optimal density of birch where growth gains of understory Douglas-fir are balanced against losses to Armillaria ostoyae (Romagn.) Herink and understory plant species diversity. We sought to find this optimal density by comparing four evenly applied birch density reduction treatments (0, 400, 1111, and 4444 retained birch stems ha?1) and an unthinned control (>7300 retained birch stems ha?1). The mortality rate of Douglas-fir due to Armillaria root disease increased non-significantly with thinning intensity. Mean diameter increment of surviving Douglas-fir improved the most where birch was completely removed, with little variation among intermediate thinning treatments. Height growth was unaffected by the thinning treatments. Diversity of cryptogams was significantly greater in the control than where all birch was removed. We suggest that the treatment with 4444 retained birch stems ha?1 provides the best balance for improving Douglas-fir growth while minimizing risk of increased Armillaria root disease and reduced understory plant diversity in young mixed stands.  相似文献   
59.
药用真菌猪苓的研究现状及应用展望   总被引:1,自引:0,他引:1  
较全面和系统地阐述了药用真菌猪苓研究的历史现状、营养及其药用价值、生物学特性、分类、栽培技术等,并对其研究中存在的问题及未来应用进行了展望.  相似文献   
60.
Honey fungi, Armillaria spp. are common and economically important pathogens of a wide range of tree species grown both in the forest and orchard cultures worldwide. Our field research in 73 forest stands across Poland has shown a common association of the mycetophagous wood nematode, Bursaphelenchus fraudulentus with Armillaria‐infected trees. The data reported here provide the first insight into the parasitic interaction of a nematode and Armillaria ostoyae. In laboratory conditions, B. fraudulentus reproduced on A. ostoyae, caused substantial damage to its mycelium and, finally, killed the whole colony within a short time. In ageing cultures, the nematode produced resting (dauer) juveniles. After artificial inoculation to Armillaria‐infected trees, the nematode population persisted under the bark, continued feeding and mass reproduction on the mycelium, and dispersed over the mycelial fans. The ability of B. fraudulentus to develop and reproduce on the surface and inside the fungal rhizomorphs suggests that these structures could facilitate the nematode dispersion in the environment. Since B. fraudulentus is not pathogenic to the host tree, its parasitic association with A. ostoyae may contribute to natural regulation of this fungus in the environment. The observed characteristics of this nematode suggest that it could potentially be used as a biocontrol agent of honey fungi in forest and orchard trees.  相似文献   
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