低磷日粮中添加植酸酶对蛋鸡肠道形态结构的影响   总被引：3，自引：0，他引：3  

邱梅平  王恬 《饲料工业》2009,30(24)

试验旨在研究在低磷的玉米-豆粕型日粮中添加植酸酶对蛋鸡肠道形态结构的影响。选用18周龄体重、产蛋周龄相近的伊莎褐蛋鸡840羽,随机分成7组:正、负对照组及在负对照组基础上分别添加300、1000和10000U/kg的包衣状植酸酶、300U/kg的粉状及300U/kg的微丸状植酸酶(试验Ⅲ、Ⅳ、Ⅴ、Ⅵ和Ⅶ组)。结果表明:与负对照组相比,Ⅳ组的空肠绒毛高度得到了显著提高(P<0.05),Ⅲ、Ⅴ、Ⅵ和Ⅶ组与之相比无显著差异(P>0.05),但分别提高了16.49%、21.80%、15.73%以及17.29%;隐窝深度、绒毛高度/隐窝深度、黏膜厚度与之相比差异不显著(P>0.05)。各试验组间以及试验组与正对照组之间亦无显著差异(P>0.05)。因此,在低磷日粮中添加植酸酶对蛋鸡肠道结构具有一定的改善作用。  相似文献   

Evaluation of equivalency values of microbial phytase to inorganic phosphorus in 22- to 42-day-old broilers     

《The Journal of Applied Poultry Research》2009,18(4):707-715

  相似文献   

蛋鸡饲料中添加植酸酶对蛋鸡生产性能的影响     

孙旺斌  毕台飞  王静 《中国农学通报》2009,25(21):33-35

摘要： 选择40周龄健康的海兰褐壳蛋鸡1200只,随机分为3组,设1个对照组和2个试验组。2个试验组饲喂在对照组基础上分别添加300、600U/kg植酸酶的日粮,其营养标准相对对照组分别降低了0.1%、0.2%的有效磷,研究产蛋鸡饲料中添加植酸酶来替代部分磷酸氢钙对产蛋鸡生产性能、蛋壳厚度、鸡蛋品质和饲料成本的影响。试验结果表明：产蛋鸡饲料中添加300U/kg植酸酶替代0.1%有效磷与对照组相比,产蛋率提高了1.8%,蛋重、蛋壳厚度、鸡蛋品质(哈氏单位) 、采食量统计上差异不显著（P>0.05）;添加600U/kg植酸酶替代0.2%有效磷与对照组相比,产蛋率提高了1.7%,但蛋壳厚度降低了2.8%,蛋重、采食量、鸡蛋品质(哈氏单位)统计上差异不显著（P>0.05）。  相似文献   

葡萄糖酸钠和植酸酶对肉鸡营养利用和屠宰性能的影响   总被引：1，自引：0，他引：1  

石杨红  郭艳丽  李发弟  史兆国  郑琛  郝正里  李少斌 《甘肃农业大学学报》2009,44(1)

用300只1周龄的Arbor Acres(AA)商品肉公鸡,采用2×3试验设计,研究了玉米豆粕型低磷饲粮中添加葡萄糖酸钠(0和2%)和植酸酶(0、500、1 000 U/kg)对肉鸡营养物质利用、胃肠道pH值和屠宰性能的影响.结果表明:在低磷饲粮中添加葡萄糖酸钠提高了肉鸡前期的干物质表观存留率(P<0.000 1)和腹脂率(P=0.063);添加植酸酶提高了肉鸡的全净膛率(P=0.065)、腿肌率(P=0.093)、腹脂率(P=0.001),降低了回肠pH值(P=0.07);葡萄糖酸钠和植酸酶在腹脂率提高上存在互作效应(P=0.043).  相似文献   

磷与植酸酶对异育银鲫磷代谢的影响   总被引：2，自引：2，他引：0  

汪益峰  周维仁  章世元  宦海林  薛飞  徐小明  丁丽  顾金 《江苏农业学报》2009,25(4)

采用二因子五水平二次正交旋转组合设计,磷水平分别为1.059%、 1.100%、 1.200%、 1.300%、 1.341%;植酸酶水平分别为0、 0.003%、 0.010%、 0.017%、 0.020%,共9组.将960尾异育银鲫随机分成9组,每组3个重复,每个重复20尾.结果表明:当磷含量为1.100%、植酸酶含量为0.020%时,生产性能最佳,增重率达26.28%,回归方程为:Y=25.14-0.99x1+0.08x2-1.39x21+0.34x22-1.10x1x2, R2=0.94*(Y表示增重率,x1表示磷含量,x2表示植酸酶含量);当磷含量为1.059%、植酸酶含量为0.01%时,磷的排放量最低,为1.09×10-2 mg/(g·d),回归方程为:Y=2.39-0.05x1+0.17x2-0.61x21-0.05x22-0.16x1x2,R2=0.91*(Y表示磷的排放量,x1表示磷含量,x2表示植酸酶含量);当磷含量为1.200%、植酸酶含量为0.010%时,能使植酸降解率达到最大值84.48%,回归方程为:Y=82.95-1.82x1+4.78x2-10.67x21-8.83x22-2.41x1x2,R2=0.91*(Y表示植酸的降解率,x1表示磷含量,x2表示植酸酶含量).  相似文献   

植酸酶和木聚糖酶对仔猪钙、磷代谢及骨骼发育的影响   总被引：3，自引：0，他引：3  

郝瑞荣  高俊杰 《饲料博览》2009,(6):1-5

试验采用完全随机设计,将90头35日龄断奶仔猪分为5组,每组设3个重复,每个重复6头,研究在饲粮中添加植酸酶和木聚糖酶对断奶仔猪钙、磷代谢及骨骼发育的影响。5组分别饲喂：①玉米-豆粕基础饲粮（正对照组,PC）;②基础饲粮降低75％磷酸氢钙（负对照组,NC）;③负对照组饲粮＋植酸酶（植酸酶组,phy）;④负对照组饲粮＋木聚糖酶（木聚糖酶组,xy）;⑤负对照组饲粮＋植酸酶＋木聚糖酶（植酸酶-木聚糖酶组,pay—xy）,植酸酶的添加量为750U·kg^-1,木聚糖酶的添加量为4000U·kg^-1。结果表明：单独添加植酸酶和木聚糖酶或两者同时添加均使磷消化率极显著提高（P〈0.01）、排出量显著降低（P〈0．05）,血液磷浓度达到正常磷水平;添加酶的各组钙的消化率显著高于负对照组（植酸酶组、植酸酶一木聚糖酶组P〈0．01;木聚糖酶组P〈0．05）;添加酶的各组猪掌骨灰分均显著高于负对照组（P〈0．05）,但与正对照组无显著差异;添加植酸酶或木聚糖酶仔猪的酿骨强度均显著高于负对照组（P〈0．05）,但木聚糖酶组猪的跟骨强度仍显著低于采食正常磷饲粮的正对照组（P〈0．05）。因此,在断奶仔猪低磷饲粮中添加植酸酶或木聚糖酶都不同程度地促进了钙、磷的消化利用和骨骼生长,但两种酶同时添加没有显著的协同效应。  相似文献   

日粮非植酸磷和基因工程酵母菌植酸酶水平对22～42日龄黄羽肉鸡生长和胫骨发育的影响     

蒋宗勇  林映才  周桂莲  李得忠  蒋守群  席鹏彬  张罕星 《中国农业科学》2009,42(12):4349-4357

 【目的】研究确定22～42日龄黄羽肉鸡非植酸磷（NPP）的需要量及毕赤酵母基因工程菌植酸酶（PHY A）的磷当量值。【方法】选用22日龄岭南黄羽肉公鸡1 320只,随机分为11个处理,每处理4个重复,每重复30只鸡。处理1为基础日粮（NPP水平为0.08%）,不添加磷酸氢钙和植酸酶;处理2～7在基础日粮中分别添加不同水平化学纯磷酸氢钙,日粮NPP水平分别为0.16%、0.24%、0.32%、0.40%、0.48%、0.56%;处理8～11在基础日粮中分别添加200、400、600 U&#8226;kg-1PHY A、400 U&#8226;kg-1美国进口植酸酶产品（PHY B）。【结果】肉鸡日增重随日粮NPP水平升高而增加,0.32%～0.56%组平均日增重显著高于对照组和0.16%组（P＜0.05）,0.48%组日增重最大;添加磷组肉鸡采食量显著高于对照组（P＜0.05）;0.40%组料重比最低,显著低于对照组（P＜0.05）;随日粮NPP水平升高肉鸡胫骨脱脂干骨重、干骨中磷含量和灰分中磷含量均显著升高,0.48%组达到最高,显著高于对照组、0.16%和0.24%组（P＜0.05）,添加磷组干骨中钙含量与灰分含量显著高于对照组（P＜0.05）。添加400、600 U&#8226;kg-1 PHY A和400 U&#8226;kg-1 PHY B显著提高了日增重、采食量和胫骨脱脂干骨重（P＜0.05）,添加2种植酸酶均显著提高了胫骨干骨中钙、磷含量与灰分中磷含量（P＜0.05）,添加600 U&#8226;kg-1 PHY A显著提高了干骨中灰分含量（P＜0.05）。【结论】22～42日龄满足黄羽肉鸡最佳生长和骨骼发育的NPP需要量为0.40%,PHY A的磷当量为685 U&#8226;kg-1。  相似文献   

Effects of Microbial Phytase Supplementation in Corn Distiller's Dried Grain with Solubles on Nutrient Digestibility and Growth Performance of Rainbow Trout,Oncorhynchus mykiss     

《Journal Of Applied Aquaculture》2013,25(3-4):83-100

Abstract

Two experiments were conducted to evaluate the effects of microbial phytase in corn distiller's dried grain with solubles (DDGS) on apparent digestibility coefficients (ADCs) and growth performance of rainbow trout, Oncorhynchus mykiss. In experiment 1, DDGS was supplemented with phytase at 0,300,600,900, and 1200 FTU/kg diet. A total of 180 fish (129.1±9.3 g) were stocked into six 140-L tanks with 30 fish/tank. Fish were assigned randomly to a reference diet and each of the 5 diets containing DDGS. Yttrium oxide was used as an inert marker. Results showed that ADCs in DDGS supplemented with different dosages of phytase were: dry matter, 49.1-58.6%; crude fat, 78.9-88.9%; crude protein, 80.0-91.9%; gross energy, 50.5-66.6%; minerals, -7.3-99.7%; and amino acids: 73.9 to 96.8%. In experiment 2, abasal diet containing 15% DDGS supplemented with lysine and methionine was used to determine if trace mineral supplemental levels in rainbow trout diets could be reduced if microbial phytase was supplemented. Six diets were made with trace mineral premix supplementation at 0.1, 0.08, 0.06, 0.04, 0.02, and 0%. Phytase was not supplemented in the basal diet, but supplemented at 500 FTU/kg diet in all other diets. Ten-week results showed that there were no significant differences in fish weight gain, feed conversion ratio, and survival (P < 0.05). There were no significant differences in fish body composition and apparent nutrient retention among fish fed all diets, except that fish fed a diet without trace mineral supplementation had the lowest zinc level and the highest manganese retention. Results indicated that phytase was effective in releasing most of minerals, and that trace mineral supplementation level could be reduced when phytase was used in rainbow trout diets.  相似文献   

Expression of recombinant phytase of Bacillus subtilis E20 in Escherichia coli HMS 174 and improving the growth performance of white shrimp,Litopenaeus vannamei,juveniles by using phytase‐pretreated soybean meal‐containing diet     

W. Cheng  C.‐S. Chiu  Y.‐K. Guu  S.‐T. Tsai  C.‐H. Liu 《Aquaculture Nutrition》2013,19(2):117-127

To produce a thermostable and neutral phytase (phy) of Bacillus subtilis E20 in Escherichia coli HMS174 and evaluate its efficiency in improving growth performance. The phy C of B. subtilis E20 was expressed in E. coli HMS 174, and then the 42‐kDa recombinant phy C was purified by Ni‐NAT and analysed by SDS–PAGE. The recombinant phy C had optimal ranges of pH of 6 ~ 7 and temperature of 50 ~ 60 °C. A thermostability analysis showed that the enzyme is a thermostable phytase, and around 33% of residual activity was detected after being incubated at 90 ~ 100 °C for 10 min. The recombinant phy C‐pretreated soybean meal for feed preparation improved white shrimp, Litopenaeus vannamei, growth and feed efficiency. Overall, the neutral and thermostable phy C is suitable for aquafeed, and it is able to improve the nutritional utilization, resulting in enhanced shrimp growth and reduced feed costs.  相似文献   

Nonphytate Phosphorus Requirement and Efficacy of a Genetically Engineered Yeast Phytase for Yellow Broilers at 22- to 42-d-Old Age     

JIANG Zong-yong  ZHOU Gui-lian  LIN Ying-cai  JIANG Shou-qun  LI De-zhong  XI Peng-bin  ZHANG Han-xing 《中国农业科学(英文版)》2011,10(2):304-311

An experiment was conducted to investigate the requirement of nonphytate phosphorus (OP) and efficacy of a genetically engineered yeast phytase (PHY A) for Lingnan yellow broilers from 22- to 42-d-old age. A total of 1320 1 -d-old male chicks were randomly divided into 11 dietary treatment groups, which consisted of 4 replicate floor pens with 30 birds per pen.The control group (treatment 1) was fed with basal diet of nPP 0.08% without dicalcium phosphate or phytase supplementation. Dietary levels of nPP were 0.16, 0.24, 0.32, 0.40, 0.48, and 0.56%, respectively, for treatments 2 to 7,through addition of dicalcium phosphate (chemistry grade) to the basal diet. Diets of treatments 8 to 11 were supplemented with PHY A at 200, 400 and 600 U kg-t, a commercial phytase product (PHY B) at 400 U kg-1 level, respectively. The birds in 0.32-0.56% nPP groups gained more than those of the other groups (P<0.05). The nPP supplementation significantly improved feed intake (P<0.05). The feed gain ratio was significantly decreased by 0.40% nPP diet compared to the control birds (P<0.05). The level of 0.48% nPP was required for optimum tibia development. The additions of PHY A at 400 and 600 U kg-1 level and PHY B all significantly improved ADG (P<0.05), ADFI (P<0.05), and dry defatted tibia weight (P<0.05).Similarly, the percentage of tibia ash was increased by 600 U kg-1 PHY A supplementation (P<0.05). The requirement of nPP for maximal ADG and highest percentage tibia ash both was 0.40%. The phosphorus equivalency value of PHY A was estimated as 685 U kg-1 for male yellow broilers of 22- to 42-d-old age.  相似文献