黑曲霉NRRL3135菌株植酸酶基因在毕赤酵母GS-115系统中的表达   总被引：1，自引：0，他引：1  

康伟  王智  詹冬玲  雷霆  冯雁 《吉林农业大学学报》2006,28(6):623-627

将黑曲S（Aspergillus riger）NRRL3135中的植酸酶基因转入毕赤酵母GS-115中,并整合到染色体DNA上,然后进行诱导表达。通过SDS-PAGE对表达产物进行鉴定,发现重组毕赤酵母GS-115分泌了1个约100kD的蛋白,分泌的蛋白较大。用脱糖基化酶endoglycosidase H降解后,形成了约50kD的蛋白,与植酸酶基因产物的理论值相符。通过对表达产物的酶学性质研究,发现该表达产物在pH2．5和pH5．5时具有较高植酸酶活性,同时该表达产物的最适温度约为55℃。这说明植酸酶得到正确的分泌表达。  相似文献   

植酸酶对3种植物性原料中植酸磷含量的影响   总被引：6，自引：0，他引：6  

曹玲  王卫民  杨承泰  杨毅 《淡水渔业》2007,37(4):52-56

利用微生物植酸酶分别对3种植物性原料(豆粕、玉米蛋白粉、小麦)进行酶处理,研究不同浓度植酸酶作用下3种植物性原料中植酸磷含量的变化。3种原料分别有6组处理(植酸酶对照0 U/kg,柠檬酸对照,500U/kg,750 U/kg,1000 U/kg,1500 U/kg),每组处理3个重复。结果表明:植酸酶能够显著降低3种植物性原料中植酸磷的含量(P<0.05),同时提升有效磷的含量。通过对磷当量的分析,本试验中以植酸酶组1000 U/kg最为经济有效。植酸酶对照组中植酸磷含量也有所下降,说明柠檬酸单独对植酸起到了一定的分解作用。  相似文献   

发芽、温度及pH对麦类籽实中植酸酶活性的影响   总被引：5，自引：0，他引：5  

马玺  单安山 《动物营养学报》2003,15(2):54-57

在25℃的条件下发芽 ,小麦东农123、小麦东农973821、大麦垦啤2号在第3天植酸酶活性达到最高 ,分别为原来的2.04、2.55、4.63倍 ;小黑麦92021、小黑麦5305及黑麦2号在第1天即达到最大 ,为发芽前的1.36、1.82和2.45倍。小黑麦5305中植酸酶的适宜温度为50℃ ,在40℃～60℃范围内可保持较高的酶活 ;适宜pH为5.5 ,低于4.0或高于7.0 ,酶活性基本丧失。温度与pH对植物植酸酶活性的影响存在互作效应  相似文献   

Effect of zinc bacitracin and phytase on growth performance,nutrient digestibility,carcass and meat traits of broilers          下载免费PDF全文

Y. A. Attia  F. Bovera  A. E. Abd El‐Hamid  A. E. Tag El‐Din  M. A. Al‐Harthi  A. S. El‐Shafy 《Journal of animal physiology and animal nutrition》2016,100(3):485-491

A total of 336 one‐day‐old Hubbard broiler chickens were randomly distributed among 8 groups, each containing six replicates (7 chickens/replicate). From 1 to 40 days of age, the groups fed the same starter, grower and finisher diets. The control group was unsupplemented; zinc bacitracin (ZnB) group received the antibiotic at 0.5 g/kg; fungal phytase (FP) groups received 250, 500 and 1000 U/kg diet of Aspergillus niger phytase (FP_250, FP_500 and FP_1000 groups), respectively; bacterial phyatse (BP) groups received 250, 500 and 1000 U/kg diet of Escherichia coli phytase (BP_250EP, BP_500EP and BP_1000EP groups) respectively. Considering the whole experimental period, body weight gain was unaffected by ZnB and different concentrations of bacterial and fungal phytase; however, the feed conversion ratio of the group fed a diet supplemented with 500 U of BP was better (p < 0.01) than those fed with a diet supplemented with 500 U of FP. BP_250 group had a higher (p < 0.05) apparent digestibility of ether extract compared to FP_250 group. In conclusion, bacterial phytase at 500 U may enhance performance of broiler chickens fed during days 1–40 of age and yield similar growth performance and economic efficiency to those of eB‐supplemented groups.  相似文献   

Supplementation of broiler diets with high levels of microbial protease and phytase enables partial replacement of commercial soybean meal with raw,full‐fat soybean          下载免费PDF全文

M. M. Erdaw  R. A. Perez‐Maldonado  P. A. Iji 《Journal of animal physiology and animal nutrition》2018,102(3):755-768

A 3 × 3 + 1 factorial, involving three levels of protease (0, 15,000 or 30,000 PROT/kg) and three levels of phytase (1,000, 2,000 or 3,000 FYT/kg), was used to evaluate the effect of replacing commercial soybean meal (SBM) with raw, full‐fat soybean (RFSB) at 75 g/kg of diet for broilers. A control diet was used for comparison. Each treatment was replicated six times, with nine birds per replicate. The concentration of trypsin inhibitors (TIs) in the test diets was approximately 10,193.4 TIU/kg. Regardless of enzyme supplementation, feed intake (FI) and body weight gain (BWG) of birds in the control group were superior to those on the test diets. Birds that received the protease‐free test diets had reduced FI and BWG, but when supplemented with protease, were similar to the control diet in BWG, FI (except 0–35 days) and feed conversion ratio (FCR). When the test diet was supplemented with elevated levels (extradose) of protease and phytase, the BWG was improved during 0–10 days (p = .05) and 0–24 days (p < .01). Regardless of protease supplementation, the weight of thighs was lower for birds fed the test diets. Birds that received the control diet had smaller weight of pancreas. Increasing the level of phytase supplementation reduced (p < .05) the weight of the pancreas. The apparent ileal digestibility (AID) of CP and AA was higher in birds on the control diets, but this was also improved in test diets by protease supplementation. The activities of trypsin (7%), general proteolytic (11%) and lipase (12%) were slightly increased because of protease supplementation. Mucosal depth and apparent villus surface areas were increased by about 2.9% and 20%, respectively, due to supplementation of elevated level of phytase. It can be concluded that RFSB could partially replace SBM in broiler diets, provided the diets are supplemented with elevated levels of protease and phytase.  相似文献   

Effects of a rice diet and phytase addition on growth performance,tissue weights,phosphorus and nitrogen retention,and on liver threonine dehydrogenase,malic enzyme and fatty acid synthase activities in broiler chicks          下载免费PDF全文

Hiroya Fujimoto  Nozomi Fujita  Ryozo Takada 《Animal Science Journal》2018,89(5):770-776

This experiment was conducted to clarify the nutritional functions of rice and phytase addition for broiler chicks. Thirty‐six 7‐day‐old male chicks (ROSS 308 strain) were assigned to one of the four treatment groups: corn‐ or rice‐based diet groups and each diet with added phytase (2000 phytase units/kg diet) groups (corn + P or rice + P groups). The non‐phytate phosphorus (npP) content in the diets with added phytase was approximately half of the requirement. Body weight gain and feed intake in the rice group was significantly higher than those in the corn group. Breast and thigh muscle weights and nitrogen retention in the rice group were significantly higher than that in the corn group. Although the efficiency of phosphorus retention (%) in the corn + P group was significantly higher than that in the corn group, no significant difference was observed between the rice and rice + P groups. Liver threonine dehydrogenase activity in the corn group was significantly higher than in the other three groups. These results indicate that rice is superior to corn as a starter diet in broiler chicks, and that phytase action in the rice‐based diet was less than that in the corn‐based diet.  相似文献   

Distribution of exogenous phytase activity in soil solid–liquid phases and their effect on soil organic P hydrolysis     

《植物养料与土壤学杂志》2017,180(1):39-48

The bioavailability and stability of organic phosphorus (P) in the soil may be affected by exogenous phytase (EPase) activity and distribution, but remain poorly understood. The distribution of EPase activity and hydrolysis ability of EPase on organic P in soil solid‐liquid phases was investigated. The EPase addition to soil suspension (1:20, w/v) from three soil types (red soil, brown soil, and cinnamon soil) under three treatments (untreated soil, removing clay from soil, and removing organic matter from soil) with different characters in the solution and solid phases was assayed. The results showed that the disappearance pattern of EPase activity from solution was similar for all soils, whereas the enzyme activity on the solid phase was dependent on soil types and treatments with the greatest in red soil and untreated soil. When EPase was added to soils, the adsorption ratio of organic matter and clay was 10 to 25% and 3 to 7%, respectively, with sorption capacity of organic matter being significantly (p < 0.05) stronger than that of clay. Additionally, soil dehydrogenase activity, which is the indicator of overall soil microbial activities, increased after EPase addition and the two enzymes showed significant negative relation in the soil suspension and solution. At the same time, the organic P decreased significantly (p < 0.05) after the addition of EPase in the soil solid, which had a varied rate under –40% after incubating 192 h, whereas organic P in the solution phase increased significantly (p < 0.05). This study demonstrated that organic matter had a strong protective and adsorptive effect on EPase effectiveness and microbes mightbe directly affect EPase longevity and decay. This finding suggests that EPase activity in the solid phase played a more important role in organic P hydrolysis.  相似文献   

Evaluation of commercial phytase enzymes on performance and tibia-breaking strength of male broiler chicks     

《The Journal of Applied Poultry Research》2010,19(4):415-421

  相似文献   

Microbial phytase activity and their role in organic P mineralization     

Muhammad Azeem  Arshad Nawaz Chaudhary  Rifat Hayat  Qaiser Hussain  Muhammad Ibrahim Tahir 《Archives of Agronomy and Soil Science》2013,59(6):751-766

Plants respond to their external environment to optimize their nutrition and production potential to minimize the food security issues and support sustainable agriculture system. Phosphorus (P) is an important nutrient for plants and is involved in plant metabolic processes. It is mostly available as orthophosphate and has a tendency to form complexes with cations. It has low mobility in soil, thus becoming unavailable for plant uptake that causes a reduction in plant growth and yield. Besides free P, phytate is the major form of organic P in soil and plant tissues. Phytases obtained from different sources, that is, plants, animals, and microorganisms, catalyze the hydrolysis of phytate and release available forms of inorganic P. The knowledge of mechanisms involved in catalytic activity of phytase obtained from microorganisms in soil is limited. This review summarizes the role of microbial phytase in releasing organic P by hydrolysis of phytate and factors affecting its activity in the soil.  相似文献   

Xylanase,Glucanase, and Phytase in the Diet of Light Laying Hens     

《The Journal of Applied Poultry Research》2019,28(4):1150-1155

  相似文献