无菌大鼠深二度烫伤感染模型机体炎性反应的研究     

李鸿雁  颜克松  马东升  战大伟  孙兆增 《中国畜牧兽医》2018,45(12):3623-3629

为探究无菌大鼠深二度烫伤感染模型机体炎性反应，试验利用无菌大鼠分别建立深二度烫伤损伤模型和烫伤后绿脓杆菌感染模型，比较这两种模型和SPF级大鼠深二度烫伤模型在愈合过程、炎性反应及病理变化等方面的异同。20只6周龄雌性Wistar无菌大鼠，在超净工作台内采用恒温恒压烫伤仪94℃作用8 s造成大鼠深二度烫伤，随机分为2组，一组伤口感染浓度为1×10⁵ CFU/mL绿脓杆菌菌液0.5 mL，一组不做处理，创面保持无菌状态，SPF级Wistar大鼠在同样条件下烫伤，创面不做处理。分别观察3组大鼠的伤口结痂时间、脱痂时间和愈合时间，并在烫伤前（0 h），烫伤后24 h、3 d和7 d时，取血清，检测炎性因子肿瘤坏死因子-α（TNF-α）、白细胞介素1α（IL-1α）、粒细胞-巨噬细胞刺激因子（GM-CSF）的变化。结果显示，感染绿脓杆菌大鼠组开始结痂所需时间显著长于SPF级大鼠组和无菌大鼠组（P<0.05）；而脱痂及愈合所需时间显著变短（P<0.05）；感染绿脓杆菌组大鼠烫伤24 h时，皮下组织中可见淋巴细胞，有慢性炎性反应；3 d时，真皮深层有化脓性感染，横纹肌周围可见大量炎细胞，烫伤7 d时，皮下组织中有轻微血管扩张充血，化脓性感染减轻，无菌烫伤大鼠与SPF级烫伤大鼠皮肤在烫伤后一周内有轻度炎性反应。3组大鼠烫伤后，血清GM-CSF、IL-1α及TNF-α水平均较烫伤前显著升高（P<0.05）；烫伤24 h时，感染绿脓杆菌大鼠组和无菌大鼠组血清IL-1α水平显著高于SPF级大鼠组（P<0.05）；无菌大鼠组血清GM-CSF水平显著高于SPF级大鼠组和感染绿脓杆菌大鼠组（P<0.05）；烫伤3 d时，染绿脓杆菌大鼠组和无菌大鼠组血清IL-1α水平显著高于SPF大鼠组（P<0.05），无菌大鼠组血清GM-CSF水平显著高于染绿脓杆菌大鼠组（P<0.05）。综上所述，无菌大鼠对烫伤刺激反应迅速强烈，血清中炎性因子迅速升高，SPF级大鼠深二度烫伤后炎性反应缓慢；无菌大鼠深二度烫伤伤口感染1×10⁵ CFU/mL绿脓杆菌，脱痂时间和愈合时间反而缩短。  相似文献   

Isolation,Identification and Drug Sensitivity Analysis of Pseudomonas aeruginosa from Minks     

SUN Na  CHEN Qiang  WEN Yong-jun  YANG Yong  WANG Meng-hang  CHENG Shi-peng 《中国畜牧兽医》2015,42(10):2788-2793

In order to provide therapeutical guidance for drug admistration,the bacteria from five dead minks suffering with typical symptoms provided by mink farms in Shandong province were isolated and identified,and the drug sensitivity was tested.The bacteria were isolated with TSA plates,and identified using biochemical methods and PCR assay.The drug sensitivity of the isolates to antimicrobial agents was investigated using K-B method.PCR was used to detect the resistance genes.A total of 5 Pseudomonas aeruginosa isolates were obtained from sick minks.The drug sensitivity results showed that 5 strains were sensitive to fluoroquinolone,the third and fourth generations cephalosporin drugs,but were resistant to aminoglycoside,tetracycline,chloramphenicol,penicillin,the first and second generations cephalosporin drugs.There were six resistance genes were detected,aadA1,aac(3')-Ⅱc,aac(6')-Ⅰb,tetA,tetK and cat2.All of the isolates were detected more than three kinds of resistance genes.The result showed that 5 strains were all Pseudomonas aeruginosa,and Pseudomonas aeruginosa was the main cause of mink hemorrhagic pneumonia.The resistance of 5 strains were very serious and mainly for multiple drug resistance phenomenon.The resistance genes detected in the mink were various,and could cause the resistance to the drugs.  相似文献   

Identification and Pathogenicity of Pseudomonas aeruginosa DJ1990 on Tail and Fin Rot Disease in Spotted Snakehead          下载免费PDF全文

Dibya J. Saikia  Pritam Chattopadhyay  Goutam Banerjee  Bandita Talukdar  Dandadhar Sarma 《Journal of the World Aquaculture Society》2018,49(4):703-714

Tail and fin rot disease (TFRD) is a big issue in the production of spotted snakehead, Channa punctata Bloch. The aims of the present study were to isolate and identify the bacterial pathogen causing TFRD, to detect histopathological changes in tissues (fin, tail, liver, and kidney), and to ascertain the antibiotic sensitivity pattern of the isolate. Out of six bacterial isolates, only the isolate DJ1990 was found to be the causal candidate of TFRD in C. punctata. Identical histopathological changes were detected in tail, fin, liver, and kidney under light and scanning electron microscope in both collected diseased fish and artificially infected fish. The isolate was identified as Pseudomonas aeruginosa strain DJ1990 (National Center for Biotechnology Information Ace. No. KX709967) based on the biochemical characterization tests and 16S rDNA sequence‐based phylogeny analysis. Artificial challenge test demonstrated that the strain DJ1990 was highly virulent (100% mortality at 48 h of postinjection period at the concentration of 1.5 × 10⁷ CFU/g of body weight) for C. punctata. The isolate exhibited sensitivity to the broad‐spectrum antibiotics but was resistant against aztreonum. To the best of our knowledge, this is the first report of P. aeruginosa as a TFRD‐causing candidate in C. punctata.  相似文献   

太湖地区不同水生植被生境下环棱螺的生存状况研究     

何连帅  谢斐  张卉  沈忱  王磊  刘茂松 《安徽农业科学》2012,(7):4150-4152

[目的]研究太湖地区不同水生植被生境下环棱螺的生存状况,为该地区环境监测提供合适的评价指标。[方法]在太湖虞望河西附近生态建设工程区的挺水植物区、沉水植物区和无植物裸底区3种水生植被生境中放养等密度的环棱螺,测定环棱螺在蓝藻爆发阶段和非蓝藻爆发阶段的存活率、超氧化物歧化酶活性、丙二醛含量和P450酶浓度,并分析所测指标与湖水氨氮含量的相关性。[结果]在蓝藻爆发阶段,挺水植物区、沉水植物区和无植物裸底区环棱螺的存活率分别为0%、55.2%和89.3%,SOD活性表现为先降后升,MDA与SOD变化趋势相反,P450酶则不断下降;在非蓝藻爆发阶段,3种生境环棱螺的存活率分别为90.6%、98.8%和99.5%,SOD活性先降后升,MDA则为先升后降,P450酶的变化没有规律。2个阶段中环棱螺SOD活性与湖水氨氮含量均显著负相关,MDA只在非蓝藻爆发期与氨氮含量显著正相关,P450酶与氨氮含量无显著相关关系,这表明用环棱螺评价水生植被对底栖动物的影响时,P450酶活性并不是合适的指标。[结论]该研究结果表明太湖地区的环棱螺体内SOD活性可以作为评价生境的指标,MDA可作为非蓝藻爆发阶段检测氨氮含量的指示物,但P450酶不能作为该地区3种生境环境污染早期诊断与预警的指标。  相似文献   

铜绿假单胞菌Z1产鼠李糖脂理化性质的研究     

张祥胜  许德军 《安徽农业科学》2012,40(34):16545-16547

[目的]为分析前期研究获得的产糖脂铜绿假单胞菌株及其发酵产物应用于石油、食品、环境和农业等领域的潜力,从而对该糖脂粗提物的理化性质进行研究。[方法]检测铜绿假单胞菌Z1发酵产生鼠李糖脂粗提物的CMC,并研究其在不同温度、盐度、酸碱度下表面活性的稳定性,同时与化学表面活性剂SDS进行比较。[结果]Z1产鼠李糖脂在-20℃常压和121℃的高温高压下表面张力均保持在40 mN/m以下,在NaCl浓度达30%情况下,表面张力也保持在37 mN/m以下,但在pH<4及pH>10的情况下,其表面张力均大于40 mN/m,分析可得其对温度、盐度表现极强的耐受性,但对酸碱度的耐受性较弱。[结论]该研究表明,鼠李糖脂表现具有极高的温度和盐度耐受性,且研究中的铜绿假单胞菌菌株或其发酵获得的糖脂粗提物具有推广应用于石油等领域的潜力。研究不足之处在于该糖脂在酸碱度的耐受性方面较弱,需进行改进。  相似文献   

大麦虫绿脓杆菌的分离鉴定和耐药性分析     

刘华栋  陆冰洋  唐娟  王彩先  李红丽  闫荫秀  詹丽娥 《中国动物保健》2012,(10):20-22

大麦虫是一种供动物食用的高蛋白虫体。本文就大麦虫突然大量死亡,通过对其体液的培养成功分离到病原菌,革兰氏染色呈红色、瑞氏和姬姆萨染色呈蓝色等着色特点,伊红美蓝琼脂培养呈红色及麦康凯琼脂培养不变色的生物学特性以及产酸、产气、糖发酵等49项生化试验,最终将分离到的细菌鉴定为绿脓杆菌。通过药敏试验,34种药物检测出其对磷霉素高敏、对庆大霉素等药物中敏,而对青霉素、红霉素等一些常见药物不敏感,旨在为临床药品的合理选用,消除动物性食品污染。  相似文献   

Phloroglucinol-Gold and -Zinc Oxide Nanoparticles: Antibiofilm and Antivirulence Activities towards Pseudomonas aeruginosa PAO1     

Fazlurrahman Khan  Min-Gyun Kang  Du-Min Jo  Pathum Chandika  Won-Kyo Jung  Hyun Wook Kang  Young-Mog Kim 《Marine drugs》2021,19(11)

With the advancement of nanotechnology, several nanoparticles have been synthesized as antimicrobial agents by utilizing biologically derived materials. In most cases, the materials used for the synthesis of nanoparticles from natural sources are extracts. Natural extracts contain a wide range of bioactive components, making it difficult to pinpoint the exact component responsible for nanoparticle synthesis. Furthermore, the bioactive component present in the extract changes according to numerous environmental factors. As a result, the current work intended to synthesize gold (AuNPs) and zinc oxide (ZnONPs) nanoparticles using pure phloroglucinol (PG). The synthesized PG-AuNPs and PG-ZnONPs were characterized using a UV–Vis absorption spectrophotometer, FTIR, DLS, FE-TEM, zeta potential, EDS, and energy-dispersive X-ray diffraction. The characterized PG-AuNPs and PG-ZnONPs have been employed to combat the pathogenesis of Pseudomonas aeruginosa. P. aeruginosa is recognized as one of the most prevalent pathogens responsible for the common cause of nosocomial infection in humans. Antimicrobial resistance in P. aeruginosa has been linked to the development of recalcitrant phenotypic characteristics, such as biofilm, which has been identified as one of the major obstacles to antimicrobial therapy. Furthermore, P. aeruginosa generates various virulence factors that are a major cause of chronic infection. These PG-AuNPs and PG-ZnONPs significantly inhibit early stage biofilm and eradicate mature biofilm. Furthermore, these NPs reduce P. aeruginosa virulence factors such as pyoverdine, pyocyanin, protease, rhamnolipid, and hemolytic capabilities. In addition, these NPs significantly reduce P. aeruginosa swarming, swimming, and twitching motility. PG-AuNPs and PG-ZnONPs can be used as control agents for infections caused by the biofilm-forming human pathogenic bacterium P. aeruginosa.  相似文献   

铜绿假单胞菌对TLR4缺失鼠的致病性研究     

白江松  张子卉  连朋敬  奚柳青  李静云  徐彤  李鸿儒  张钟方  乔健 《畜牧兽医学报》2022,53(3):904-912

本研究旨在了解TLR4信号转导在铜绿假单胞菌感染所致急性肺损伤的发病机理中的作用.通过使用Tlr4基因突变致正常TLR4信号转导缺失的C3H/HeJ小鼠,评估TLR4信号转导在调节铜绿假单胞菌引起的急性肺损伤中的作用.选用6～8周龄、雄性、TLR4缺陷型(C3H/HeJ)和SPF级野生型(C3H/HeN)的小鼠各21只...  相似文献   

不同磷源及其配比对铜绿微囊藻生长和摄磷效应的影响   总被引：4，自引：0，他引：4  

丁飞飞  黄鹤忠  陈金凤  徐汗福  范皖苏  何华敏 《水生态学杂志》2010,31(3):32-36

在实验室条件下研究了无机磷(磷酸氢二钾)与2种有机磷(β-甘油磷酸钠、三磷酸腺苷钠)不同配比对铜绿微囊藻(Microcystis aeruginosa)的生长效应以及微囊藻摄取不同磷形态[总溶解磷(total dissolved phosphorus, TSP)、溶解反应磷(soluble reactive phosphorus, SRP)和溶解有机磷(dissolved organic phosphorus, DOP)]的规律.结果表明,当无机磷与有机磷配比为6∶1时,对铜绿微囊藻生长最有利,对数生长期可维持6～8 d,其最大生物量达2.586×106个/mL;培养基中以有机磷作为唯一磷源时,铜绿微囊藻的生长最差;2种有机磷(β-甘油磷酸钠、三磷酸腺苷钠)对铜绿微囊藻生长的效果相似,铜绿微囊藻对SRP的摄取速度越快,其生长速率也越高(R=0 9995);待水中可利用磷耗尽后,β-甘油磷酸钠会在碱性磷酸酶作用下转化成可利用磷供藻生长,而三磷酸腺苷可直接分解成磷酸基供铜绿微囊藻生长.  相似文献   

In vivo control and in vitro antifungal activity of rhamnolipid B,a glycolipid antibiotic,against Phytophthora capsici and Colletotrichum orbiculare     

Beom Seok Kim  Jung Yeop Lee  Byung Kook Hwang 《Pest management science》2000,56(12):1029-1035

The glycolipid antibiotic rhamnolipid B isolated from Pseudomonas aeruginosa strain B5 was evaluated for in vitro antifungal activity and in vivo control against phytophthora blight and anthracnose under glasshouse conditions. Rhamnolipid B showed antifungal activity against Cercospora kikuchii, Cladosporium cucumerinum, Colletotrichum orbiculare, Cylindrocarpon destructans, Magnaporthe grisea and Phytophthora capsici. Microscopic observation revealed that the high level of antifungal activity (10 µg ml ⁻¹) against P capsici was mainly due to a lytic effect on zoospores. Zoospore lysis began in the presence of 10 µg ml ⁻¹ of rhamnolipid B and most of the zoospores were collapsed at 25 µg ml ⁻¹. Rhamnolipid B showed inhibitory activity against the germination of zoospores and hyphal growth of P capsici at concentrations of 50 µg ml ⁻¹. Spore germination of the anthracnose plant pathogen C orbiculare was also inhibited in the presence of 50 µg ml ⁻¹ of rhamnolipid B, although hyphal growth was not affected at this concentration. In the glasshouse, the efficacy of rhamnolipid B against phytophthora blight was similar to that of metalaxyl on pepper plants when treated just before inoculation with P capsici. Treatment with either at 500 µg ml ⁻¹ completely protected pepper plants from phytophthora blight. Rhamnolipid B also suppressed the development of C orbiculare infection on leaves of cucumber plants. © 2000 Society of Chemical Industry  相似文献