双歧杆菌对脾虚小鼠肠道菌群的影响     

赵红梅  赵建  冯建远 《勤云标准版测试》2006,(10)

使昆明小白鼠致脾虚,用双歧杆菌纯制剂治疗后,检测结肠内容物和粪便的肠杆菌、肠球菌、双歧杆菌和乳酸杆菌数量的变化,并结肠进行组织学检查。结果显示:小鼠脾虚后肠杆菌、肠球菌数量明显增多(P<0.05),双歧杆菌、乳酸杆菌数量大量减少(P<0.01);用双歧杆菌纯制剂治疗6d～12d后,肠杆菌、肠球菌数量减少(P<0.05),双歧杆菌、乳酸杆菌大量增多(P<0.01);治疗组结肠组织学观察显示,肠壁肌层厚度增加,杯状细胞数量增多,肠黏膜微绒毛排列紊乱显著改善。  相似文献   

Effects of intramammary infusion of Bifidobacterium breve on mastitis pathogens and somatic cell response in quarters from dairy cows with chronic subclinical mastitis     

Hajime Nagahata  Takuma Mukai  Yo Natsume  Miyuki Okuda  Tatsuya Ando  Keiichi Hisaeda  Satoshi Gondaira  Hidetoshi Higuchi 《Animal Science Journal》2020,91(1)

The present study assessed the effects of intramammary infusion of Bifidobacterium breve (B. breve) on mastitis‐causing pathogens and on the somatic cell counts (SCC) in lactating cows with chronic subclinical mastitis. The bacteriological cure rates of 42 quarters from 42 cows infected with Staphylococcus aureus, Corynebacterium bovis, coagulase‐negative staphylococci, and environmental streptococci were 18.2% (2/11), 14.3% (1/7), 58.8% (10/17), and 28.6% (2/7), respectively, on day 14 after B. breve infusion. In a second trial, B. breve was infused into 18 quarters from 18 cows with chronic subclinical mastitis from which pathogens had not been isolated; the rates of quarters showing SCC > 50 × 10⁴ cells/ml prior to B. breve infusion that decreased to < 30 × 10⁴ cells/ml after infusion were significantly (p < .01) increased to 61.1% (11/18) on day 14 compared to that prior to infusion (0/18). The intramammary infusion of B. breve appears to be a non‐antibiotic approach for elimination of minor pathogens and decreasing SCC in quarters with chronic subclinical mastitis in dairy cows.  相似文献   

Effects of synbiotic-based Bifidobacterium animalis in female rats experimentally infected with Toxoplasma gondii     

Cláudia Mello RibeiroVeruska Maia Costa  Maria Isabel Franchi Vasconcelos GomesMarjorie Assis Golim  José Rafael ModoloHélio Langoni 《Comparative immunology, microbiology and infectious diseases》2011,34(2):111-114

The aim of this study was to assess the effects of a synbiotic composed of Bifidobacterium animalis and fructooligosaccharides on female rats infected with Toxoplasma gondii. Female Wistar rats, treated or not with dexamethasone, were daily supplemented with synbiotics for 21 days. After 15 days of supplementation, the rats were orally infected with 10⁴T. gondii bradyzoites. Blood samples were collected to measure the levels of IFN-γ, IL-10 and T. gondii antibodies. All synbiotic-supplemented rats survived until the end of the experiment; however, non-supplemented dexamethasone-treated rats died between the fifth and the eighth days after T. gondii infection. Dexamethasone-treated rats supplemented with synbiotics (P < 0.05) were capable of synthesizing IFN-γ, and this immunological response was essential to ensure their survival. In addition, brain cysts were found in one rat not supplemented with synbiotics. Results suggest that the synbiotic composed of B. animalis and fructooligosaccharides may be beneficial to toxoplasmosis control.  相似文献   

温度对双歧杆菌及其产品质量影响研究进展     

周发友  吴晓玉  刘好桔  王园秀 《广东农业科学》2013,40(13):110-112

双歧杆菌作为消化道的生理性益生菌,已被广泛应用于食品、医药、饲料等行业.由于双歧杆菌的生长和存活条件比较苛刻,在生产与贮藏过程中保证功能菌的活菌数量和产品质量的问题亟待解决,其中温度是对产品影响最为重要的因素之一.从双歧杆菌生长和其产品品质两方面阐述了温度对其影响的概况.  相似文献   

消化道环境胁迫对青春双歧杆菌KLDS2.0003粘附能力影响     

孟祥晨  李馨  朱德全  范修海 《东北农业大学学报》2013,44(6)

确定消化道环境胁迫对青春双歧杆菌KLDS2.0003粘附能力、菌体表面性质及菌体细胞膜脂肪酸组成影响.采用体外方法模拟消化道环境,以Caco-2细胞作为粘附模型,青春双歧杆菌KLDS2.0003相继经过模拟唾液、模拟胃液以及模拟肠液处理后,测定经历模拟消化道环境前后存活率、粘附能力、自动聚集能力和表面疏水性,同时采用扫描电镜和透射电镜观察菌体形态以及采用GS-MS法测定菌体处理前后细胞膜脂肪酸组成变化.结果表明,青春双歧杆菌KLDS2.0003经模拟消化道环境胁迫后,粘附能力下降,菌体表面疏水性、自动聚集能力菌下降、菌体细胞形态发生改变,膜不饱和脂肪酸与饱和脂肪酸比例增加,而且有一种新长链不饱和脂肪酸C18:2n6c产生.结果显示,消化道环境胁迫会降低青春双歧杆菌KLDS2.0003粘附能力,同时菌体自动聚集能力和表面疏水性均降低,三者之间呈现一定相关性,此外,在消化道环境胁迫条件下,膜脂肪酸组成发生改变,诱导产生一种新长链不饱和脂肪酸C18:2n6,c推测该菌通过调节膜脂肪酸组成变化以抵抗消化道环境中不利因素.  相似文献   

产细菌素双歧杆菌L-SN对酸奶后酸化及品质的影响     

尚楠  刘丽莎  任发政  李平兰 《中国农业大学学报》2013,18(4):178-182

选择产细菌素双歧杆菌L-SN菌株与保加利亚乳杆菌和嗜热链球菌混合发酵剂配伍共同发酵生产酸奶,通过测定4℃贮藏条件下酸奶的pH、可滴定酸度、活菌数、持水力和黏度等指标的变化探讨产细菌素双歧杆菌对酸奶后酸化效果及品质的影响.结果显示:双歧杆菌L-SN的加入能有效抑制酸奶的后酸化,以添加水平为(2～5)×106 cfu/mL为最佳,可在20 d贮藏期内维持酸度为100～110°T、pH 4.1～4.2、活菌数≥106 cfu/mL,并能显著提高酸奶的持水力和黏度,赋予酸奶良好的色泽、口感和组织状态.  相似文献   

响应面法优化乳双歧杆菌Z-1冷冻干燥保护剂配方     

徐显睿  李翠凤  隋勇军  张宗博  杨洪来  李道河  陈政言  张兰威 《乳业科学与技术》2020,43(3):6-11

采用响应面法对乳双歧杆菌Z-1冷冻干燥保护剂配方进行优化。通过Plackett-Burman试验与最陡爬坡试验确定影响乳双歧杆菌Z-1冷冻干燥后活菌数的显著因素及最优响应值区间，再通过Box-Benhnken试验优化得到乳双歧杆菌Z-1冷冻干燥保护剂最佳配方。结果表明：海藻糖、蔗糖、L-半胱氨酸盐酸盐添加量是影响乳双歧杆菌Z-1冷冻干燥后活菌数的显著因素；经响应面试验设计建立活菌数和海藻糖、蔗糖、L-半胱氨酸盐酸盐添加量的回归模型，得到乳双歧杆菌Z-1冷冻干燥保护剂最佳配方为海藻糖添加量200.51 g/L、蔗糖添加量46.16 g/L、L-半胱氨酸盐酸盐添加量2.31 g/L，其他成分不变（脱脂乳粉添加量100 g/L、乳清蛋白粉20 g/L、甘油4 g/L、异抗坏血酸钠10 g/L、谷氨酸钠8 g/L）。  相似文献   

利用新分离青春双歧杆菌制作酸奶的研究     

张洁  刘宇超  于颖  徐桂花 《乳业科学与技术》2010,33(4):156-159

研究新分离青春双歧杆菌在酸奶制作中的应用,采用驯化后的青春双歧杆菌与嗜热链球菌混合发酵的方法制作双歧酸奶。以产酸能力、活菌数量、风味为产品指标。结果表明：当混合菌种配比（双歧杆菌/嗜热链球菌）为1：1时,不仅缩短了发酵时间,双歧杆菌活菌数也达到了10^9cfu/mL,而且酸奶的品质和口味均有所改善。  相似文献   

A Probiotic Cocktail as a Growth Promoter in Nile Tilapia (Oreochromis niloticus)     

M. S. Ayyat  Howaida M. Labib  Hemat K. Mahmoud 《Journal Of Applied Aquaculture》2013,25(3):208-215

The aim of this study was to evaluate Lactobacillus acidophilus, Streptococcus thermophilus, Bifidobacterium bifidum, and yeast Saccharomyces cerevisiae singly and in combination as probiotic supplements in Nile tilapia (Oreochromis niloticus) fingerling diets. All the probiotic-supplemented diets resulted in growth higher than the control. Best growth rate, food consumption, and food conversion (P < 0.05) were in the group fed a cocktail of the three bacteria. Feed cost, return on weight gain, and profit margin increased in fish groups fed diets inoculated with probiotics. Supplementation with Lactobacillus acidophilus, Bifidobacterium bifiduim, and the three-bacteria cocktail were most effective in eliminating mortality in an A. hydrophila challenge.  相似文献   

动物双歧杆菌基因组的研究进展     

朱德全  尹红  张路路  张武  张跃华  刘德江 《安徽农业科学》2017,45(15)

动物双歧杆菌是一种常用的益生双歧杆菌菌株,常以益生菌的形式添加到乳制品和其他食品中。该研究就动物双歧杆菌基因组的最新研究进展、重要功能基因和比较基因组进行了综述,以期进一步推广应用该菌株。  相似文献