全文获取类型
收费全文 | 6431篇 |
免费 | 325篇 |
国内免费 | 732篇 |
专业分类
林业 | 450篇 |
农学 | 446篇 |
基础科学 | 284篇 |
1394篇 | |
综合类 | 2416篇 |
农作物 | 337篇 |
水产渔业 | 197篇 |
畜牧兽医 | 1200篇 |
园艺 | 413篇 |
植物保护 | 351篇 |
出版年
2024年 | 36篇 |
2023年 | 153篇 |
2022年 | 229篇 |
2021年 | 264篇 |
2020年 | 240篇 |
2019年 | 260篇 |
2018年 | 216篇 |
2017年 | 317篇 |
2016年 | 343篇 |
2015年 | 292篇 |
2014年 | 362篇 |
2013年 | 463篇 |
2012年 | 479篇 |
2011年 | 489篇 |
2010年 | 383篇 |
2009年 | 399篇 |
2008年 | 340篇 |
2007年 | 380篇 |
2006年 | 359篇 |
2005年 | 234篇 |
2004年 | 224篇 |
2003年 | 144篇 |
2002年 | 126篇 |
2001年 | 103篇 |
2000年 | 110篇 |
1999年 | 103篇 |
1998年 | 58篇 |
1997年 | 69篇 |
1996年 | 57篇 |
1995年 | 38篇 |
1994年 | 25篇 |
1993年 | 37篇 |
1992年 | 37篇 |
1991年 | 22篇 |
1990年 | 15篇 |
1989年 | 21篇 |
1988年 | 15篇 |
1987年 | 11篇 |
1986年 | 6篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1983年 | 2篇 |
1981年 | 4篇 |
1980年 | 4篇 |
1979年 | 2篇 |
1977年 | 1篇 |
1974年 | 1篇 |
1956年 | 5篇 |
1955年 | 2篇 |
排序方式: 共有7488条查询结果,搜索用时 15 毫秒
61.
Xinpeng Yang Yue Feng Yang Li Dake Chen Xuanyan Xia Jialian Li Fenge Li 《Reproduction in domestic animals》2021,56(3):416-426
Sertoli cells are the only somatic cells in the seminiferous epithelium which directly contact with germ cells. Sertoli cells exhibit polarized alignment at the basal membrane of seminiferous tubules to maintain the microenvironment for growth and development of germ cells, and therefore play a crucial role in spermatogenesis. Androgens exert their action through androgen receptor (AR) and AR signalling in the testis is essential for maintenance of spermatogonial numbers, blood–testis barrier integrity, completion of meiosis, adhesion of spermatids and spermiation. In the present study, we demonstrated that AR gene could promote the proliferation of immature porcine Sertoli cells (ST cells) and the cell cycle procession, and accelerate the transition from G1 phase into S phase in ST cells. Meanwhile, miR-124a could affect the proliferation and cell cycle procession of ST cells by targeting 3′-UTR of AR gene. Furthermore, AR bound to the RNF4 via AR DNA-binding domain (DBD) and we verified that RNF4 was necessary for AR to regulate the growth of ST cells. Above all, this study suggests that AR regulates ST cell growth via binding to RNF4 and miR-124a, which may help us to further understand the function of AR in spermatogenesis. 相似文献
62.
阐明长期有机物料施肥下土壤CO2排放特征及其影响机制以及碳库管理指数对黄土高原旱作农业区固碳减排及施肥模式选择的影响尤为重要。基于2012年设置在陇中黄土高原旱作区的长期定位试验,通过不施肥(CK)、氮肥(NF)、有机肥(OM)、秸秆(ST)、有机肥结合无机肥(OMNF)5个处理,测定并计算了2018年不同施肥措施下全年土壤CO2排放、作物碳排放效率和碳库管理指数的变化,并运用结构方程模型分析了0~30 cm土壤温度、水分、微生物量碳氮、易氧化有机碳、蔗糖酶、脲酶与土壤CO2排放速率的关系。结果表明:1)与不施肥相比,秸秆、有机结合无机肥和有机肥处理使生育期土壤CO2排放平均速率提高了42.72%、30.82%和29.79%,秸秆、有机肥处理分别使生育期土壤CO2排放量显著提高36.35%、32.45%(P<0.05),有机结合无机肥处理使碳排放效率显著降低41.10%(P<0.05);2)有机物料处理均能显著提高0~5 cm土层易氧化有机碳、微生物量碳氮、蔗糖酶活性和碳库管理指数,相比不施肥和氮肥处理,有机结合无机肥处理分别使0~30 cm土壤碳库管理指数提高127.41%,99.33%(P<0.05);3)结构方程模型表明,环境因子对土壤CO2排放速率的总解释度为53%,对土壤CO2排放速率总效应较大的影响因素包括土壤温度(2.36)、微生物量碳(1.59)和土壤水分(1.18),且均间接地影响着土壤CO2排放速率,土壤温度促进了微生物量碳和蔗糖酶活性的提高,微生物量碳促进了微生物量氮和易氧化有机碳的增加。综合来看,有机结合无机肥处理可以提升土壤碳库管理指数,保持微生物活性,增加作物产量,降低土壤碳排放效率,是陇中黄土高原旱作农业区比较适宜的农田培肥措施。 相似文献
63.
64.
为从“源头控制”畜禽粪便减排,提高生猪养殖效益,本试验将同日龄、初始体重相近的(P>0.05)育肥猪40头分成4个组,1个对照组(正常饲喂)和3个试验组,每组10头,试验组分限饲10%组、限饲20%组、限饲30%组。试验组限饲50 d后,解除限制饲喂30 d,在管理条件基本相同情况下发现限饲期间,试验各组猪的粪便产生量、末重和平均日增重均显著低于对照组(P<0.05);解除限饲后,与对照组相比,限饲20%组和限饲30%组猪的粪便排放量显著降低,平均日增重显著增高(P<0.05),而限饲20%组猪的试验末重略高于对照组且差异不显著,限饲30%组则显著低于对照组。结果表明限饲20%组的猪粪便排泄量减少,补偿生长方式相对效果较好。 相似文献
65.
Hiroshi BANNAI Manabu NEMOTO Koji TSUJIMURA Takashi YAMANAKA Ken MAEDA Takashi KONDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):309-311
To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4
(EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer:
MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more
strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera
from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%),
whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer
ELISA. 相似文献
66.
Effects of different dl‐selenomethionine and sodium selenite levels on growth performance,immune functions and serum thyroid hormones concentrations in broilers
下载免费PDF全文
![点击此处可从《Journal of animal physiology and animal nutrition》网站下载免费的PDF全文](/ch/ext_images/free.gif)
This trial was conducted in a 2 × 3 + 1 factorial arrangement based on a completely randomized design to evaluate the effects of different dl ‐selenomethionine (dl ‐Se‐Met) and sodium selenite (SS) levels on growth performance, immune functions and serum thyroid hormones concentrations in broilers. A total of 840 Ross 308 broilers (7 days old) were allocated by body weight to seven treatments (three replicates of 40 birds each treatment) including (1) basal diet (containing 0.04 mg of selenium (Se)/kg; control) without supplementary Se; (2, 3 and 4) basal diet + 0.05, 0.15 or 0.25 mg/kg Se as SS; (5, 6 and 7) basal diet + 0.05, 0.15 or 0.25 mg/kg Se as dl ‐Se‐Met. The experiment lasted 42 days. The results revealed that dietary Se supplementation improved (p < 0.05) average daily gain, feed efficiency, immune organ index, serum immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM) and triiodothyronine (T3) concentrations and decreased (p < 0.01) thyroxine (T4)/T3 ratio in serum compared with the control. Broilers receiving the dl ‐Se‐Met‐supplemented diets had higher (p < 0.05) feed efficiency, thymus index, the amounts of IgA, IgG, IgM and T3 as well as lower (p < 0.05) serum T4 concentrations and T4/T3 ratio than those consuming the SS‐supplemented diets. Serum IgA and IgM levels of broilers fed 0.15 mg Se/kg were significantly higher (p < 0.05) than those of broilers fed 0.05 or 0.25 mg Se/kg. In summary, we concluded that dl ‐Se‐Met is more effective than SS in increasing immunity and promoting conversion of T4 to T3, thus providing an effective way to improve the growth performance of broilers. Besides, based on a consideration of all experiment indices, 0.15 mg Se/kg was suggested to be the optimal level of Se supplementation under the conditions of this study. 相似文献
67.
Objective To improve the isolation rate and identification procedures for Haemophilus parasuis from pig tissues. Design Thirteen sampling sites and up to three methods were used to confirm the presence of H. parasuis in pigs after experimental challenge. Procedure Colostrum‐deprived, naturally farrowed pigs were challenged intratracheally with H parasuis serovar 12 or 4. Samples taken during necropsy were either inoculated onto culture plates, processed directly for PCR or enriched prior to being processed for PCR. The recovery of H parasuis from different sampling sites and using different sampling methods was compared for each serovar. Results H parasuis was recovered from several sample sites for all serovar 12 challenged pigs, while the trachea was the only positive site for all pigs following serovar 4 challenge. The method of solid medium culture of swabs, and confirmation of the identity of cultured bacteria by PCR, resulted in 38% and 14% more positive results on a site basis for serovars 12 and 4, retrospectively, than direct PCR on the swabs. This difference was significant in the serovar 12 challenge. Conclusion Conventional culture proved to be more effective in detecting H parasuis than direct PCR or PCR on enrichment broths. For subacute (serovar 4) infections, the most successful sites for culture or direct PCR were pleural fluid, peritoneal fibrin and fluid, lung and pericardial fluid. For acute (serovar 12) infections, the best sites were lung, heart blood, affected joints and brain. The methodologies and key sampling sites identified in this study will enable improved isolation of H parasuis and aid the diagnosis of Glässer's disease. 相似文献
68.
69.
70.
随着经济的快速发展,温室气体的排放量不断增加,加之人类对自然资源的利用强度逐渐增加,导致全球生态系统的固碳能力减弱,大气中的温室气体浓度达到新高,所造成的温室效应已经成为国际社会普遍关注的重大全球性问题。中国草地碳汇资源得天独厚,发展草原碳汇经济成为履行国际承诺、打造碳汇新经济、建设美丽中国的重要载体。综述中国草地固碳减排现状及其影响因素,包括草地碳汇和家畜生产减排研究、气候变化背景下的草地碳汇、人工草地建设等方面,并提出中国草地固碳减排发展建议,以期为中国实现碳达峰、碳中和及草地固碳减排的贡献提供理论基础,为推动我国社会高质量发展、创造高品质生活提供坚实的技术支撑。 相似文献