氯化胆碱对玉米幼苗清除自由基的作用     

何若天  覃伟 《广西农业生物科学》2000,19(4):255-257

经叶面喷施以不同浓度 ( 0 ,1 0 0 ,30 0 ,50 0 ,70 0 ,1 0 0 0 mg/L)氯化胆碱 ( CC)处理后的玉米幼苗幼叶提取液能不同程度地清除体外系统产生的超氧物自由基 ( O- 2 )、羟自由基 (· OH)和脂质自由基( R· )等 ,对上述自由基的清除率分别为 4 .2 7%～ 2 1 .68% ,1 1 .78%～ 2 8.4 %和 7.84 %～ 35.1 5%。其中以用 50 0和 70 0 mg/L的 CC处理者清除效果最佳  相似文献   

氯化胆碱对水稻幼苗内源激素及生长的影响     

郑桂萍  李金峰 《黑龙江八一农垦大学学报》1995,8(2):65-68

不同浓度的氯化胆碱溶液浸种明显提高了水稻根、芽中内源生长素（ＩＡＡ）、赤霉素（ＧＡ３）、玉米素（Ｚ）的含量，而对脱落酸（ＡＢＡ）含量影响甚微；加速了种子内部的物质转化，使种子中的可溶性糖、氨基酸含量显著增加；从而促进了种子萌发，加速了幼苗生长。  相似文献   

菠菜胆碱单加氧酶基因cDNA的克隆及重组植物表达载体的构建   总被引：1，自引：0，他引：1       下载免费PDF全文

柳娜  王蒂  司怀军  李东魁  刘海英  李有忠 《西北农业学报》2007,16(6):107-112

  相似文献   

植物生长调节剂（组合）对南方地区淮山药产量及品质的影响   总被引：4，自引：1，他引：3  

温国泉  钦洁  韦威旭 《广西农业科学》2010,41(9):955-959

为探索不同植物生长调节剂种类及浓度对南方地区淮山药生产的影响效果,以＂桂淮2号＂淮山药为供试品种,研究矮壮素（CCC）与羟季铵·萘合剂（氯化胆碱CC＋萘乙酸NAA）对南方地区淮山药产量及品质的影响。结果表明,喷施CCC2250mg·L^-1淮山药产量最高,达到33255.15kg·ha^-1,比对照及仅喷0.15%KH2PO4处理增产15.20%和13.22%;喷施CCC2250mg·L^-1有利于提高淮山药鲜薯的干物质含量,增加淮山药块茎干样的水溶性浸出物和醇溶性浸出物含量,促进了淮山药淀粉含量的提高,并同时能维持较高的蛋白质含量,而对可溶性总糖含量影响不显著,从而保证了其具有良好的食用品质和风味。  相似文献   

植物胆碱单氧化酶的生物信息学分析     

冯潜弄庆媛  麦秀英周虹陈劲松  周嘉裕廖海 《中国农学通报》2012,28(24):194-200

为了研究胆碱单氧化物酶的抗逆机理,运用NCBI、Expasy、Psort、NetPhosK、TMHMM、NPSA网站和MEGA5、DNAman软件,对已在GenBank数据库中注册中的甜菜、菠菜、四翅滨藜草、山菠菜、苋菜等植物胆碱单氧化酶的氨基酸序列进行生物信息学分析。结果表明：植物胆碱单氧化酶中Ala、Ser、Leu等氨基酸含量较丰富,属于稳定蛋白;不同植物胆碱单氧化酶的氨基酸序列具有较高的同源性(81.52%),均含有转运肽区、2Fe-S中心区和非血红素Fe离子结合区;分子进化树揭示胆碱单氧化酶有较强的保守性,可作为植物遗传分化和分子进化研究的重要依据;分子中不存在跨膜结构域,与疏水区域预测结果一致;可能受蛋白激酶C磷酸化,位点为Ser326;无规卷曲是多肽链中大量的结构元件;包含2个功能结构域,分属于Rieske家族与SRPBCC家族。本研究结果为开展胆碱单氧化酶的酶学特性及甘氨酸甜菜碱生物合成的分子机理研究提供理论参考。  相似文献   

Cross‐resistance of Echinochloa species to acetolactate synthase inhibitor herbicides          下载免费PDF全文

Soo‐Hyun Lim  Min‐Jung Yook  Jin‐Won Kim  Do‐Soon Kim 《Weed Biology and Management》2017,17(2):91-102

This study was conducted to evaluate the cross‐resistance of acetolactate synthase (ALS) inhibitors with different chemistries, specifically azimsulfuron (sulfonylurea), penoxsulam (triazolopyrimidine sulfonanilide) and bispyribac‐sodium (pyrimidinyl thio benzoate), in Echinochloa oryzicola and Echinochloa crus‐galli that had been collected in South Korea and to investigate their herbicide resistance mechanism. Both Echinochloa spp. showed cross‐resistance to the ALS inhibitors belonging to the above three different chemistries. In a whole plant assay with herbicides alone, the resistant/susceptible ratios for azimsulfuron, penoxsulam and bispyribac‐sodium were 12.6, 28.1 and 1.9 in E. oryzicola and 21.1, 13.7 and 1.8 in E. crus‐galli, respectively. An in vitro ALS enzyme assay with herbicides showed that the I ₅₀‐values of the resistant accessions were approximately two‐to‐three times higher than the susceptible accessions, with no statistical difference, suggesting that the difference in ALS sensitivity cannot explain ALS inhibitor resistance in Echinochloa spp. for azimsulfuron, penoxsulam and bispyribac‐sodium. A whole plant assay with fenitrothion showed that the GR ₅₀‐values significantly decreased in both the resistant E. oryzicola and E. crus‐galli accessions when azimsulfuron, penoxsulam and bispyribac‐sodium were applied with the P450 inhibitor, while no significant decrease was observed in the susceptible accessions when the P450 inhibitor was used. Thus, these results suggest that ALS inhibitor cross‐resistance for azimsulfuron, penoxsulam and bispyribac‐sodium is related to enhanced herbicide metabolism.  相似文献   

Transformation of Brassica oleracea var. capitata with bacterial betA gene enhances tolerance to salt stress     

R. C. Bhattacharya  M. Maheswari  V. Dineshkumar  P. B. Kirti  S. R. Bhat  V. L. Chopra 《Scientia Horticulturae》2004,100(1-4):215-227

The bacterial betA gene for biosynthesis of glycinebetaine was transferred to cabbage (Brassica oleracea var. capitata) cultivar ‘Golden Acre’ through Agrobacterium-mediated transformation of hypocotyl explants. Transgenic status was established through Southern hybridization and mRNA expression in the shoots. The transformants exhibited higher tolerance to NaCl stress compared to untransformed parent plants. In physiological assessment of salinity tolerance, transgenics showed better growth response and greater stability in maintaining plant water relations at increasing levels of salinity. These results demonstrate that engineering glycinebetaine biosynthetic pathway into cabbage can lead to enhanced salt tolerance.  相似文献   

Spontaneous ultraweak photon emission from rice (Oryza sativa L.) and paddy weeds treated with a sulfonylurea herbicide     

Hidehiro Inagaki  Guang-Xi Wang  Kimihiko Kato  Hiroyuki Iyozumi 《Pesticide biochemistry and physiology》2007,89(2):158-162

All living organisms spontaneously generate ultraweak photon emissions, which originate from biochemical reactions in cells. Current research uses the ultraweak photon emissions from organisms as a novel tool to investigate the physiological states of plants. In this study, we found ultraweak photon emissions from leaf segments of rice and several paddy weed species treated with a sulfonylurea herbicide. There is a definite difference in photon emissions among plant species, and rice (Oryza sativa), barnyardgrass (Echinochloa crus-galli) and Cyperus serotinus showed extremely strong enhancement of photon emissions. Photon emissions from these three species treated with sulfonylurea herbicide were suppressed when the leaf segments were treated with the cytochrome P450 monooxygenase (P450) inhibitors, piperonyl butoxide and malathion. These results suggest that P450 inhibitors affect the ultraweak photon emissions from plants.  相似文献   

基于转录组测序解析草地贪夜蛾对溴氰虫酰胺的解毒代谢分子机制     

杨媛雪  王爱玉  张芸  张开军  薛超  刘怀  赵鸣  张建华 《植物保护学报》2023,50(3):800-811

为阐明草地贪夜蛾Spodoptera frugiperda对溴氰虫酰胺的解毒代谢分子机制,通过LC₅₀的溴氰虫酰胺诱导草地贪夜蛾3龄幼虫后,利用酶活测定和转录组测序鉴定解毒代谢相关基因,并采用实时荧光定量PCR技术对细胞色素P450单加氧酶(cytochrome P450 monooxygenase,P450)基因进行验证分析。结果表明,经LC₅₀的溴氰虫酰胺处理后,草地贪夜蛾3龄幼虫体内3种解毒代谢酶活性较对照均有所升高,但仅P450活性较对照显著升高,而谷胱甘肽S-转移酶和羧酸酯酶与对照无显著差异。经LC₅₀的溴氰虫酰胺处理后草地贪夜蛾3龄幼虫转录组中共筛选到1 408个差异表达基因,其中上调表达的基因有935个,下调表达的基因有473个。药物代谢-细胞色素P450通路、药物代谢-其他酶通路及细胞色素P450对异生物质的代谢通路中有超过20个基因存在差异表达。在草地贪夜蛾转录组中筛选鉴定到121个P450基因,其中,属于CYP2、CYP3、CYP4以及Mito家簇的基因分别有9、45、58和9个,而经LC_5...  相似文献   

Molecular cloning and gene expression of two cytochrome P450s from permethrin-resistant Culex quinquefasciatus larvae     

Shinji Kasai  Indira S Weerashinghe  Minoru Yamakawa  Toshio Shono 《Pest management science》1999,55(5):601-602

As part of a continuing study of factors influencing the development of pesticide resistance in insects, two new cytochrome P450s of the CYP6 family have been identified.  相似文献