H2O2对Pb胁迫下小麦幼苗抗氧化酶及细胞活性的影响     

叶亚新  陆丹华 《湖北农业科学》2013,52(5)

以小麦(Triticum aestivum L.)为试验材料,采用完全营养液进行培养,外加适当浓度的过氧化氢(H2O2),同时以不加H2O2为对照,再以不同浓度Pb溶液培养进行Pb胁迫,检测小麦幼苗过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性及丙二醛(MDA)含量、根系活力及微核率等指标的变化,以探究外源H2O2对Pb胁迫下小麦抗氧化酶及其细胞活性的影响.结果表明,Pb胁迫下小麦幼苗的生长代谢受到一定程度的抑制,主要表现为根系活力下降,POD、SOD、CAT活性增强,MDA含量和微核率上升;经适当浓度H2O2处理后,Pb胁迫小麦幼苗中POD、SOD、CAT活性及根系活力与对照相比有不同程度地上升,MDA含量及微核率下降,这表明外加H2O2对Pb胁迫小麦所受的伤害有一定的缓解作用,可增强小麦幼苗对重金属Pb的抗性.  相似文献   

杭州种子植物三新记录种     

黎念林  陈晓玲  谭远军  朱春艳 《安徽农业科学》2013,(18):7745-7745,7753

2012年春,杭州植物园的调查人员在余杭中泰乡的铜铃桥周围山上发现了荞麦叶大百合[Cardiocrinum cathayanum(E.H.Wilson)Stearn]、六角莲[Dysosma pleiantha(Hance)Woodson]和灯台莲[Arisaema sikokianum Franch.Sav.var.serratum(Makino)Hand.-Mazz.]。经结果比对,发现它们为杭州的新分布种。  相似文献   

改良甲亚胺H酸法快速测定豆奶中硼砂   总被引：1，自引：0，他引：1  

郑鸿雁  黄翠萍 《安徽农业科学》2013,(27):11153-11155

[目的]建立快速测定豆奶中硼砂的新方法。[方法]以市售豆奶为样品,经碱化、碳化、灰化,以5ml5％HCl溶解其灰分,使硼砂转化为硼酸根离子,用甲亚胺H酸作显色剂,在波长420n／n处测定其吸光值。[结果]试验表明,样品中硼酸浓度在0—0．500mg／ml线性良好,线性相关系数为0．9991,其加标回收率为97．04％-99．58％,相对标准偏差（RSD）为1．53％。3．69％。最低检出限浓度1．83mg／kg。与姜黄素分光光度法比较,差异无显著性（t=1．800,P〉0．05）。[结论]该法所需试验设备简单,避免了繁琐的浓缩步骤,节约了大量乙醇,灵敏度及准确性均能满足分析要求,适用于大批量豆奶中硼砂的检测。  相似文献   

Protein supply in organic broiler diets     

M. Eriksson  L. Waldenstedt  B. Engström  K. Elwinger 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2013,63(4):211-219

Abstract

Organic feed legislation, long-rearing periods and high requirements of sulphur-rich amino acids (AAs) complicate the composition of a well-balanced organic diet for broilers. To evaluate the effect of protein and AA composition, three different diets were fed to 180 Ross broilers, divided over 45 pens. The diets comprised a low crude protein (CP) and AA diet, a high CP diet aiming at AA levels used in conventional production and a low protein diet supplemented with lysine, methionine and threonine up to levels of the high protein diet. Chickens were immunologically challenged with an inactivated infectious bursal disease (IBD) vaccine; antibody titres, heterophil/lymphocyte (H/L) ratios, lymphoid organ weights and digestibility coefficients were recorded. In general, bird performance improved with increasing dietary CP and AA levels. Dietary treatments did not significantly affect any of the measured immune or stress-related parameters.  相似文献   

An enzyme-linked immunosorbent assay for detection of avian influenza virus subtypes H5 and H7 antibodies     

Trine H Jensen  Gitte Ajjouri  Kurt J Handberg  Marek J Slomka  Vivien J Coward  Martine Cherbonnel  Véronique Jestin  Peter Lind  Poul H J?rgensen 《Acta veterinaria Scandinavica》2013,55(1):84

Background

Avian influenza virus (AIV) subtypes H5 and H7 attracts particular attention because of the risk of their potential pathogenicity in poultry. The haemagglutination inhibition (HI) test is widely used as subtype specific test for serological diagnostics despite the laborious nature of this method. However, enzyme-linked immunosorbent assays (ELISAs) are being explored as an alternative test method.H5 and H7 specific monoclonal antibodies were experimentally raised and used in the development of inhibition ELISAs for detection of serological response specifically directed against AIV subtypes H5 and H7. The ELISAs were evaluated with polyclonal chicken anti-AIV antibodies against AIV subtypes: H1N2, H5N2, H5N7, H7N1, H7N7, H9N9, H10N4 and H16N3.

Results

Both the H5 and H7 ELISA proved to have a high sensitivity and specificity and the ELISAs detected H5 and H7 antibodies earlier during experimental infection than the HI test did. The reproducibility of the ELISA’s performed at different times was high with Pearson correlation coefficients of 0.96-0.98.

Conclusions

The ELISAs are a potential alternative to the HI test for screening of large amounts of avian sera, although only experimental sera were tested in this study.  相似文献   

陕西省avian-like H1N1猪流感病毒的遗传进化分析     

任娟娟  王晶钰  刘红彦  刘万华  武宁  邱渊皓  唐攀  江跃德 《中国农业科学》2013,46(18):3901-3913

【目的】监测陕西省猪流感的流行与传播情况,为猪流感研究提供参考。【方法】从生猪养殖场和屠宰场表观健康猪中随机采取猪鼻/气管拭子样本,经SPF鸡胚分离,对HA阳性样本进行猪流感病毒RT-PCR鉴定。测定分离株全基因序列,通过MEGA4构建基因进化树,并进行遗传变异分析。【结果】从500份样本中分离鉴定出6株H1N1流感病毒,病毒分离率为1.2%。获得6个核苷酸相似性为98.3%—99.1%的分离株全基因序列。遗传进化分析表明6个分离株都位于avain-like H1N1猪流感病毒谱系,与中国江苏,辽宁,山东和香港等地H1N1猪流感病毒株同源性达97%—99%。6个分离株的8个基因片段均在蛋白受体结合位点、潜在糖基化位点等处发生核苷酸变异,这将会影响猪流感病毒的抗原特性、宿主适应性和致病力。【结论】首次从西北地区表观健康猪中分离到H1N1猪流感病毒,并获得6个分离株的全基因序列。遗传进化分析表明,6个分离株为avain-like H1N1猪流感病毒, 且多处氨基酸位点发生变异。  相似文献   

禽流感H9亚型一步法RT-PCR快速检测试剂盒的研制及应用     

程福亮  梁瑾  范群平  陈甜甜  宿志瑞  聂兆晶  谷巍 《中国畜牧兽医》2013,(1):50-53

为建立一种简单、快速、灵敏、准确的禽流感H9检测方法,本研究根据GenBank中收录的禽流感H9病毒高度保守的基因序列,设计1对H9的特异性引物,建立了一步法快速检测禽流感的PCR,对反应条件进行优化后,组装成快速检测试剂盒,并对临床收集的疑似禽流感病料进行检测。结果显示,试验成功建立了禽流感H9病毒一步法RT-PCR检测方法,在此基础上完成了试剂盒的组装,对30份疑似禽流感病料的检出率高达98%以上,检测灵敏度达10^1.5ELD₅₀,稳定性良好, 冷冻保存期达12个月。经证实该试剂盒具有操作简便、经济、快速、灵敏度高、特异性强、重复性好、稳定性高等优点,值得推广应用。  相似文献   

Spatiotemporal Characteristics of Reference Evapotranspiration and Its Sensitivity Coefficients to Climate Factors in Huang-Huai-Hai Plain,China   总被引：2，自引：0，他引：2  

YANG Jian-ying  LIU Qin  MEI Xu-rong  YAN Chang-rong  JU Hui  XU Jian-wen 《中国农业科学(英文版)》2013,(12):2280-2291

Climate change will have important implications in water shore regions,such as Huang-Huai-Hai（3H） plain,where expected warmer and drier conditions might augment crop water demand.Sensitivity analysis is important in understanding the relative importance of climatic variables to the variation in reference evapotranspiration（ET 0）.In this study,the 51-yr ET 0 during winter wheat and summer maize growing season were calculated from a data set of daily climate variables in 40 meteorological stations.Sensitivity maps for key climate variables were estimated according to Kriging method and the spatial pattern of sensitivity coefficients for these key variables was plotted.In addition,the slopes of the linear regression lines for sensitivity coefficients were obtained.Results showed that ET 0 during winter wheat growing season accounted for the largest proportion of annual ET 0,due to its long phenological days,while ET 0 was detected to decrease significantly with the magnitude of 0.5 mm yr-1in summer maize growing season.Solar radiation is considered to be the most sensitive and primarily controlling variable for negative trend in ET 0 for summer maize season,and higher sensitive coefficient value of ET 0 to solar radiation and temperature were detected in east part and southwest part of 3H plain respectively.Relative humidity was demonstrated as the most sensitive factor for ET 0 in winter wheat growing season and declining relativity humidity also primarily controlled a negative trend in ET 0,furthermore the sensitivity coefficient to relative humidity increased from west to southeast.The eight sensitivity centrals were all found located in Shandong Province.These ET 0 along with its sensitivity maps under winter wheat-summer maize rotation system can be applied to predict the agricultural water demand and will assist water resources planning and management for this region.  相似文献   

Antioxidant effect of carnosine on H9N2 swine influenza virus-induced acute lung injury in mice     

WANG Guo-hua  WANG Cun-lian  TIAN Shu-fei  LIU Bao-jian  XU Ming-ju  WEI Dong  ZHANG Rui-hua  XU Tong 《园艺学报》2013,29(12):2235-2239

AIM:To investigate the antioxidant effect of carnosine on H9N2 swine influenza virus (H9N2-SIV)-induced acute lung injury (ALI). METHODS:One hundred and fifty SPF female BALB/c mice (6 to 8 weeks old) were randomly divided into control group, ALI group and carnosine intervention group with 50 each. The mice in control group were inoculated intranasally with normal allantoic fluid of chick embryos. The mice in ALI group were inoculated intranasally with allantoic fluid containing H9N2-SIV. The mice in carnosine group were treated with H9N2-SIV plus carnosine. On the 2nd, 4th, 6th, 8th and 14th days after treatment, 8 mice in each group were killed to observe the pathological changes of the lung. Meanwhile, the activity of superoxide dismutase (SOD) and myeloperoxidase (MPO), the content of malondialdehyde (MDA) and the wet weight/dry weight ratio (W/D) of the lung tissues were determined. RESULTS:Carnosine alleviated the symptom of the mice induced by H9N2-SIV infection, and increased the viability of the mice. In carnosine intervention group, edema degree of the lung (W/D) was apparently reduced (P<0.05). The pathological changes were alleviated on the 6th and 8th days of the experiment. On the same days, the content of MDA was lower obviously (P<0.05) and the activity of SOD was improved remarkably (P<0.05). On the 4th day of the experiment, the activity of MPO was reduced apparently (P<0.05) and continuously decreased on the 6th and 8th days (P<0.05). CONCLUSION: Carnosine protects the mice from acute lung injury induced by H9N2-SIV infection and increases the viability by reducing the content of MDA, lowering the activity of MPO, increasing the activity of SOD and inhibiting the production of free radicals and lipid peroxidation.  相似文献   

牛不同组织中稳定性同位素氢、氧、硫组成探讨   总被引：2，自引：0，他引：2  

孙丰梅  石光雨  王慧文  杨曙明 《核农学报》2012,26(8):1148-1153

为研究牛不同组织中氢、氧、硫稳定同位素的组成变化情况以及品种对其的影响,利用同位素比率质谱仪测定了不同部位脱脂牛肉、牛尾毛、血液、肝脏中的δ2H值和δ34S值及牛肉抽提水中的δ2H,δ18O值。结果显示,品种因素不影响组织中的硫同位素组成,但对氢同位素组成的影响尚不确定;肌肉不同部位、血液、肝脏、牛尾毛之间的δ2H值、δ34S值均有极显著差异,但各组织间的δ34S、δ2H值相关关系不明显;肉品水中δ2H值与δ18O值存在较强的相关关系。这些结果说明稳定性同位素氢、硫在牛不同组织中的分馏效应是不一致的,在进行牛肉溯源研究时,应根据研究目的和研究对象选择合适的组织。  相似文献