Effect of Repeated Arthrocentesis on Cytologic Analysis of Synovial Fluid in Dogs     

R.I.M. Berg  J.E. Sykes  P.H. Kass  W. Vernau 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2009,23(4):814-817

Background: Serial arthrocentesis and synovial fluid examination can be used to monitor treatment efficacy in immune-mediated polyarthritis (IMPA), but whether this procedure induces inflammation that interferes with test result interpretation is unknown.
Objectives: The aim of this study was to determine the effect of repeated arthrocentesis on synovial fluid cytology in healthy dogs.
Animals: Nine healthy client-owned dogs.
Methods: Prospective study. Arthrocentesis was performed under sedation on 4 joints (both carpi, 1 tarsus, 1 stifle) on each dog every 3 weeks, a total of 4 times. Automated cell counts were done on stifle fluid, smears were made, and differential cell counts done on smears from all joints. Slides were evaluated microscopically for erythrocyte numbers, total nucleated cell count, differential cell count, and cell morphology. Data were analyzed by 2-way analysis of variance.
Results: A total of 144 synovial fluid samples were examined. Repeated arthrocentesis was not associated with increases in synovial fluid neutrophil numbers. Mild mononuclear inflammation was detected in 13 samples from 6 dogs.
Conclusions and Clinical Importance: Serial arthrocentesis at 3-week intervals can rarely be associated with mild mononuclear joint inflammation, but does not appear to induce neutrophilic inflammation, at least in healthy dogs, and can be useful to monitor treatment response in canine IMPA.  相似文献   

Comparison of aspiration and nonaspiration techniques for obtaining cytologic samples from the canine and feline spleen     

Casey J. LeBlanc  Laurie L. Head  Michael M. Fry 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2009,38(2):242-246

Background: Ultrasound‐guided fine needle aspiration of the spleen is commonly used in the diagnostic evaluation of veterinary patients. Techniques using suction delivered through a 6–20‐cm³ syringe are the most commonly described means of obtaining cytologic samples of the spleen. Comparison studies of various human lesions have shown nonaspiration techniques to produce equal or superior cytologic specimens with less blood than specimens obtained using aspiration techniques. Objective: The purpose of this study was to compare the quality of splenic cytology specimens obtained using aspiration and nonaspiration techniques. Methods: Client‐owned dogs (n=24) and cats (n=7) receiving an abdominal ultrasound at the University of Tennessee College of Veterinary Medicine were enrolled in the study between January and June 2005. Samples were obtained from patients with and without sonographic splenic abnormalities. Two clinical pathologists, working independently and blinded to the method of sample collection, graded the cytologic specimens using a subjective scoring system for cellularity, amount of blood, and preservation of cellular morphology. Results: Agreement between the 2 independent observers was good. Direct comparison of the 2 techniques showed that samples obtained by the nonaspiration method had higher cellularity (P=.0002), less blood (P=.0023), and similar cell morphology (P=1.0000) compared with samples obtained by the aspiration method. Conclusion: These results suggest the nonaspiration technique is a superior method for obtaining a high‐quality cytologic specimen from the canine and feline spleen.  相似文献   

甜瓜属人工异源四倍体(Cucumis hytivus)染色体组间重组的 细胞学及分子标记研究   总被引：4，自引：0，他引：4  

庄飞云  CHEN Jin-feng  钱春桃  罗向东  雷春 《中国农业科学》2005,38(3):582-588

 采用细胞学和分子标记方法对甜瓜属人工异源四倍体（C. hytivus Chen and Kirkbride，2n = 4x = 38）的亲本染色体组间的交换重组进行了研究。通过对减数分裂中期I染色体行为观察，在108个花粉母细胞中有50个细胞具有多价体，占46.3%，染色体的平均构型为0.56I+17.36II+0.35III+0.26IV+0.046V+0.056VI，表明两亲本染色体组间发生了广泛的重组交换。通过对446条随机引物进行筛选，有5条引物扩增出6条C. hytivus特征带。选取其中3条转换成SCAR标记，对13种甜瓜属不同基因型材料的DNA进行扩增，结果仅有SAP-03/700标记表现为特异性。进一步分析这一标记在人工异源四倍体（C. hytivus）、栽培黄瓜（C. sativus var. sativus）及野生黄瓜（C. sativus var. hardwickii）中扩增出的3条不同分子量条带序列，发现两端序列较为一致，中间部分不同，与黄瓜线粒体基因组序列有200 bp左右的大小相同，但方向相反。  相似文献   

新疆不同抗寒性小麦品种越冬过程中受冻时期的细胞学观察     

王佩芝  王燕凌  张慧  简令成  孙龙华 《新疆农业大学学报》1990,(3)

越冬冻害是新疆冬小麦生产中很严重的自然灾害,严重时死苗面积达20％以上。因此,防止越冬冻害是发展新疆冬小麦生产的关键问题。而对冬小麦越冬冻害的原因一直存在不同的看法:一是,冻害是由于冬季严寒,由最低温度造成。另一是,越冬过程中天气发生暂时性转暖,尔后又遇寒流,使麦田发生冻融交替。以致在寒流再度到来时,虽未超过该品种能忍受的低温极限,也会发生严重冻害。在某些地区和某些年份可能是主要的因素。第三种是以上两种看法的综合。为此,查明越冬冻害时期,对冻害原因的判断具有重要作用。因此,我们试图通过对新疆冬小麦越冬过程中的细胞学观察,探讨越冬中的冻害问题,为防御冻害提供依据。  相似文献   

5种甘蓝型油菜细胞质雄性不育系的细胞学观察   总被引：2，自引：0，他引：2  

胡永敏  董军刚  孟倩  郭英芬  董振生 《西北农业学报》2012,21(7):95-99

采用石蜡制片方法,对不缺绿ogu、陕3A型、nap、pol、758A细胞质雄性不育系及其保持系的小孢子发生和花粉发育过程进行细胞学观察和比较,以确定这些不育系的败育时期和细胞学特征。结果表明,不缺绿型ogu CMS花药败育受阻于四分体至单核花粉时期,败育特点是四分体时期持续较长,绒毡层延迟退化。陕3A型、nap、Pol CMS花粉败育发生在孢原细胞时期,少数花药会继续发育产生有活力花粉粒,表现微粉现象。758A的败育时期在孢原细胞时期,败育特点为没有造孢细胞和壁细胞的分化,不能形成药室和花粉粒,败育比较彻底。  相似文献   

Intra-abdominal mass aspirate from an alopecic dog     

Brazzell JL  Borjesson DL 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2006,35(2):259-262

An 11-year-old female Yorkshire terrier was presented to the University of Minnesota Veterinary Medical Center for evaluation of a palpable intra-abdominal mass and alopecia. Abdominal ultrasonography revealed a large, complex, cavitary mass in the left caudal region of the abdomen. A fine needle aspirate of the mass was collected. A population of markedly pleomorphic, large, round to polygonal cells were found singly and in small noncohesive aggregates. The cells contained scant, clear to blue-gray cytoplasm, large, round to oval nuclei, and distinctly stippled to reticular chromatin. Cytologic findings were consistent with a tumor of ovarian origin, with a primary differential diagnosis of germ cell tumor. Hormonal analysis of serum revealed a marked increase in 14-OH-progesterone concentration (2.71 ng/mL, reference interval 0.05-0.69 ng/mL). Ovariohysterectomy was performed, and the mass was found to be in the area of the left ovary. Histologic evaluation of the reproductive tract confirmed a diagnosis of left ovarian dysgerminoma. Based on immunohistochemical stains, the tumor was negative for c-kit (CD117c) and single cells were positive for neuron-specific enolase. A right ovarian cyst and squamous metaplasia of the right uterine horn also were diagnosed. The cyst was presumed to be the source of 14-OH-progesterone, which likely resulted in the squamous metaplasia and dermatopathy. Three months after surgery, the progesterone concentration had returned to normal and the alopecia had nearly resolved. Dysgerminomas in dogs are reported rarely, but have a distinctive, recognizable, cytologic appearance and should be included in the differential diagnosis of an intra-abdominal mass in a reproductively intact female dog.  相似文献   

Cytology of an Undifferentiated Carcinoma Developed in Athymic Nude Mice Inoculated With High Passages of a Pig Cell Line (PFT)     

Bundza A  Bouillant AM 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1979,8(1):35-38

  相似文献   

Analysis of transtracheal aspirates and pleural fluid from clinically healthy llamas (Llama glama)     

Gerros TC  Andreasen CB 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1999,28(1):29-32

Seventeen clinically healthy adult llamas were used to study the characteristics of transtracheal aspirates (TTA) and pleural fluid samples. Results of complete blood counts, fibrinogen determination and thoracic radiographs were within normal limits prior to sampling. Cytologic evaluation of TTA revealed the majority of cells were vacuolated macrophages (60-100%), with 0-40% neutrophils, and fewer lymphocytes (0-1%), eosinophils (0-3%), and ciliated respiratory epithelial cells (0-10%). In TTA from 10 of 17 llamas, neither aerobic nor anaerobic bacteria were isolated. Bacteria isolated in pure culture from TTA were similar to isolates found in clinically healthy animals of other species, and included Acinetobacter sp., Staphylococcus sp. and Bacillus sp. Results (mean +/- SD) of pleural fluid analyses were: total nucleated cell count 576 +/- 361/microliter; specific gravity 1.0133 +/- 0.002; glucose concentration 135.1 +/- 9.02 mg/dL; and lactate concentration 2.95 +/- 1.34 mg/dL. Pleural fluid total protein concentrations determined by refractometry ranged from < 2.5 to 3.5 g/dL. The refractive index ranged from 1.3396 to 0.0013. In pleural fluid, small lymphocytes were the predominant cell type.  相似文献   

小麦×大麦杂种后代早熟衍生系的选择和鉴定     

刘淑会  陈新宏  蔡振虎  武军  赵继新  杨群慧 《山西农业大学学报(自然科学版)》2011,31(2):142-145

早熟是大麦的突出优良性状,通过普通小麦与栽培大麦杂交,可把大麦的早熟性状导入到普通小麦中。为了获得确定的小-大麦早熟新种质,以中早熟小麦品种小偃22作对照,对小-大麦杂种回交后代分离群体进行选择,筛选出比对照早熟3~5 d的2个遗传稳定衍生系:WB0528和WB0647。这2个早熟株系具有抽穗与大麦基本同期,灌浆快的特点。经过细胞学和染色体组原位杂交(GISH)鉴定,2个株系均为附加了两条能够相互配对,且携带有含早熟性状大麦染色体的二体异附加系。  相似文献   

彩叶草株型矮化的化学控制及其细胞学特征初探     

郭兆武  金建军  俞健 《中国农学通报》2007,23(11):261-261

为了探索彩叶草株型矮化的化学控制,以其扦插幼苗为材料,通过对其株高及其细胞学特性研究,发现叶面喷施15%多效唑可湿性粉剂800倍液和5%烯效唑可湿性粉剂7000倍液对彩叶草株型具有显著的矮化作用;叶面喷施50%CCC水剂400倍液,矮化效果不显著。细胞学观察表明,喷施多效唑和烯效唑,其茎秆表皮细胞长径显著变短,短径无显著变化;茎秆表皮毛显著变短,每个表皮毛的细胞数显著减少;茎秆筛管直径显著变小。多效唑和烯效唑对其细胞伸长和分裂有抑制作用,限制了筛管营养物质的运输,抑制了植株其他部位的生长,这些是使彩叶草株型矮化的主要原因。喷施CCC对彩叶草无显著控矮化作用,但有相应的细胞学变化,其原因有待进一步研究。结果表明,烯效唑对彩叶草的催衰老作用较小,细胞学性状也较稳定,植株较光滑,具有良好的观赏价值,是一种较好的彩叶草控矮化剂。  相似文献