Cytologic appearance of a keloidal fibrosarcoma in a dog   总被引：1，自引：1，他引：0  

Little LK  Goldschmidt M 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2007,36(4):364-367

A 5-year-old neutered male, mixed-breed dog was presented with a single 4-mm, nodular, firm, haired subcutaneous mass on the left flank that had been present for approximately 2 weeks. Cytologic preparations of the mass revealed many spindle cells, few mast cells, rare eosinophils, rare macrophages, abundant hyalinized collagen, and moderate numbers of erythrocytes. The spindle cells were oval to fusiform, with oval nuclei, finely stippled to lacy chromatin, 1-5 variably sized prominent nucleoli, and moderate to abundant cytoplasm with indistinct cell borders, wispy cytoplasmic extensions, and occasionally, fine magenta granulation. The cell population exhibited moderate anisocytosis, moderate anisokaryosis, and rare binucleation. The eosinophilic material occurred both in large angular aggregates with blunt ends and in amorphous aggregates with fine wispy projections. Histologic findings were consistent with a keloidal fibrosarcoma. To the authors' knowledge, this report is the first to describe the cytomorphologic characteristics of a keloidal fibrosarcoma in a dog.  相似文献   

Prostatic sarcomatoid carcinoma in a dog: cytologic and immunohistochemical findings     

Pinto da Cunha N  Ghisleni G  Romussi S  Caniatti M 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2007,36(4):368-372

An 8-year-old neutered male Boxer was presented with tenesmus, hemorrhagic urethral discharge, and dysuria. Abdominal ultrasound and radiographic examinations revealed irregular prostatic enlargement. Laparotomy was performed and intraoperative cytology was done on imprint smears of a biopsy specimen obtained from a prostatic mass. The cytologic preparation was highly cellular and contained a predominant population of atypical, large, loosely cohesive spindle cells, with rare multinucleated cells and mitotic figures. The cytologic findings were consistent with undifferentiated sarcoma. At necropsy, a large cystic prostatic mass and numerous satellite nodules in the soft tissues around the pelvis were found. On histologic examination the tumor was composed primarily of bundles of neoplastic spindle cells. Rare pseudo-acinar structures and signet-ring cells also were observed. On immunohistochemical examination, the neoplastic cells co-expressed cytokeratin and vimentin. Based on histologic and immunohistochemical findings, the tumor was diagnosed as primary prostatic sarcomatoid carcinoma. This is a rare tumor in dogs, in which biphasic morphology of epithelial and mesenchymal cells can complicate the diagnosis, requiring immunochemical stains for confirmation.  相似文献   

种衣剂17号包衣对小麦苗期白粉菌发育影响的研究   总被引：12，自引：0，他引：12  

李金玉  康振生 《中国农业科学》1995,28(4):60-68

 采用光镜和电镜技术，研究了种衣剂17号包衣对小麦苗期白粉菌发育的影响。结果表明，种衣剂对白粉菌分生孢子萌发和附着胞形成无影响，但可增大附着胞的畸形率和降低入侵率；每一侵染点仅能产生初生吸器，并且吸器发育受抑制而呈畸形，吸器外间质内沉积有电子致密度高的物质；菌丝生长严重受抑，其顶端明显膨大；菌丝细胞壁和吸器体壁呈不规则加厚；受侵寄主细胞的坏死现象普遍，这与抗病品种表现出的过敏性坏死反应极为相似。以上结果表明，种衣剂17号不仅可直接作用于白粉菌，而且可能通过影响寄主而间接影响该病菌。  相似文献   

甜瓜属人工异源四倍体(Cucumis hytivus)染色体组间重组的 细胞学及分子标记研究   总被引：4，自引：0，他引：4  

庄飞云  CHEN Jin-feng  钱春桃  罗向东  雷春 《中国农业科学》2005,38(3):582-588

 采用细胞学和分子标记方法对甜瓜属人工异源四倍体（C. hytivus Chen and Kirkbride，2n = 4x = 38）的亲本染色体组间的交换重组进行了研究。通过对减数分裂中期I染色体行为观察，在108个花粉母细胞中有50个细胞具有多价体，占46.3%，染色体的平均构型为0.56I+17.36II+0.35III+0.26IV+0.046V+0.056VI，表明两亲本染色体组间发生了广泛的重组交换。通过对446条随机引物进行筛选，有5条引物扩增出6条C. hytivus特征带。选取其中3条转换成SCAR标记，对13种甜瓜属不同基因型材料的DNA进行扩增，结果仅有SAP-03/700标记表现为特异性。进一步分析这一标记在人工异源四倍体（C. hytivus）、栽培黄瓜（C. sativus var. sativus）及野生黄瓜（C. sativus var. hardwickii）中扩增出的3条不同分子量条带序列，发现两端序列较为一致，中间部分不同，与黄瓜线粒体基因组序列有200 bp左右的大小相同，但方向相反。  相似文献   

雄性不育和可育大麦花药和花粉的细胞学比较研究   总被引：1，自引：0，他引：1  

刘凤珍  韩海  栗仲兴  屠骊珠  李勇 《华北农学报》1992,(1)

本文对大麦雄性不育和可育花药形态和花粉发育进行了细胞胚胎学比较研究,结论如下: 1.可育和不育大麦的花药在外部形态上有明显区别,主要在于不育花药基部成为戟形,且开花时花药变得瘦小。 2.可育大麦的花粉发育类同于一般的禾本科植物。而不育花粉不能发育到二核期,并很快解体形成“无花粉型”花粉;其小孢子和雄配子体发育过程中有几种异常现象:(1)早期发生异常;(2)绒毡层过早退化;(3)属绒毡层解体较晚者,小孢子能发育到二细胞阶段,但形态扭曲,内含物贫乏。 3.可育的药壁发育正常同于单子叶型,直到2细胞花粉粒时期绒毡层才全部退化。而雄性不育药壁发育异常,主要体现在绒毡层早期退化或药壁分化不完全。  相似文献   

一个小麦-中间偃麦草二体代换系的鉴定与分析     

李小军  姜小苓  董娜  李淦  冯素伟  胡铁柱  茹振钢  杨永乾 《麦类作物学报》2014,34(3):318-322

中间偃麦草在小麦改良中具有重要利用价值。本研究利用基因组原位杂交(GISH)、高分子量谷蛋白亚基电泳(SDS-PAGE)和分子标记技术对小麦-中间偃麦草衍生系中209进行鉴定。结果表明,中209的42条染色体中含有2条中间偃麦草染色体,是小麦-中间偃麦草二体代换系;分子标记检测发现,小麦7A染色体上的6个SSR引物Xcfa2028、Xwmc422、Xwmc65、Xbarc127、Xbarc174和Xgwm60在小麦亲本合作2号中均扩增出清晰的DNA条带,而在中间偃麦草和中209中均未扩增出任何条带,表明中209可能缺少了小麦7A染色体;小麦第7部分同源群的SSR标记Xwmc488和STS标记Xmag1715分别在中209中扩增出中间偃麦草的特异带,推断其含有的中间偃麦草染色体与小麦第7同源群染色体存在部分同源关系;SDSPAGE表明中209含有5+10亚基。  相似文献   

新疆不同抗寒性小麦品种越冬过程中受冻时期的细胞学观察     

王佩芝  王燕凌  张慧  简令成  孙龙华 《新疆农业大学学报》1990,(3)

越冬冻害是新疆冬小麦生产中很严重的自然灾害,严重时死苗面积达20％以上。因此,防止越冬冻害是发展新疆冬小麦生产的关键问题。而对冬小麦越冬冻害的原因一直存在不同的看法:一是,冻害是由于冬季严寒,由最低温度造成。另一是,越冬过程中天气发生暂时性转暖,尔后又遇寒流,使麦田发生冻融交替。以致在寒流再度到来时,虽未超过该品种能忍受的低温极限,也会发生严重冻害。在某些地区和某些年份可能是主要的因素。第三种是以上两种看法的综合。为此,查明越冬冻害时期,对冻害原因的判断具有重要作用。因此,我们试图通过对新疆冬小麦越冬过程中的细胞学观察,探讨越冬中的冻害问题,为防御冻害提供依据。  相似文献   

Comparison of aspiration and nonaspiration techniques for obtaining cytologic samples from the canine and feline spleen     

Casey J. LeBlanc  Laurie L. Head  Michael M. Fry 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2009,38(2):242-246

Background: Ultrasound‐guided fine needle aspiration of the spleen is commonly used in the diagnostic evaluation of veterinary patients. Techniques using suction delivered through a 6–20‐cm³ syringe are the most commonly described means of obtaining cytologic samples of the spleen. Comparison studies of various human lesions have shown nonaspiration techniques to produce equal or superior cytologic specimens with less blood than specimens obtained using aspiration techniques. Objective: The purpose of this study was to compare the quality of splenic cytology specimens obtained using aspiration and nonaspiration techniques. Methods: Client‐owned dogs (n=24) and cats (n=7) receiving an abdominal ultrasound at the University of Tennessee College of Veterinary Medicine were enrolled in the study between January and June 2005. Samples were obtained from patients with and without sonographic splenic abnormalities. Two clinical pathologists, working independently and blinded to the method of sample collection, graded the cytologic specimens using a subjective scoring system for cellularity, amount of blood, and preservation of cellular morphology. Results: Agreement between the 2 independent observers was good. Direct comparison of the 2 techniques showed that samples obtained by the nonaspiration method had higher cellularity (P=.0002), less blood (P=.0023), and similar cell morphology (P=1.0000) compared with samples obtained by the aspiration method. Conclusion: These results suggest the nonaspiration technique is a superior method for obtaining a high‐quality cytologic specimen from the canine and feline spleen.  相似文献   

Effect of Repeated Arthrocentesis on Cytologic Analysis of Synovial Fluid in Dogs     

R.I.M. Berg  J.E. Sykes  P.H. Kass  W. Vernau 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2009,23(4):814-817

Background: Serial arthrocentesis and synovial fluid examination can be used to monitor treatment efficacy in immune-mediated polyarthritis (IMPA), but whether this procedure induces inflammation that interferes with test result interpretation is unknown.
Objectives: The aim of this study was to determine the effect of repeated arthrocentesis on synovial fluid cytology in healthy dogs.
Animals: Nine healthy client-owned dogs.
Methods: Prospective study. Arthrocentesis was performed under sedation on 4 joints (both carpi, 1 tarsus, 1 stifle) on each dog every 3 weeks, a total of 4 times. Automated cell counts were done on stifle fluid, smears were made, and differential cell counts done on smears from all joints. Slides were evaluated microscopically for erythrocyte numbers, total nucleated cell count, differential cell count, and cell morphology. Data were analyzed by 2-way analysis of variance.
Results: A total of 144 synovial fluid samples were examined. Repeated arthrocentesis was not associated with increases in synovial fluid neutrophil numbers. Mild mononuclear inflammation was detected in 13 samples from 6 dogs.
Conclusions and Clinical Importance: Serial arthrocentesis at 3-week intervals can rarely be associated with mild mononuclear joint inflammation, but does not appear to induce neutrophilic inflammation, at least in healthy dogs, and can be useful to monitor treatment response in canine IMPA.  相似文献   

6-甲氨基嘌呤诱导栉孔扇贝三倍体的细胞学机理   总被引：11，自引：1，他引：11  

杨爱国  王清印  孔杰  刘志鸿  刘萍  王如才  姜明  孟瑞萍 《海洋水产研究》2000,21(1):22-26

以６０ｍｇ／Ｌ的６－甲氨基嘌呤（简称６－ＤＭＡＰ）诱发栉孔扇贝三倍体时,在荧光显微镜下观察受精卵的染色体行为以及核相组成,对照三倍体率和孵化率,探讨了不同减数分裂期６－ＤＭＡＰ处理对染色体倍数性的影响。结果表明,处理５￣１５ｍｉｎ可见３种类型图像,第１种图像可见第一极体和一个雌性原核及雄性原核;第２种图像可见第一极体和两雌性原核及一个雄性原核;第３种图像可见两个雌性原核和一个雄性原枋,随着处理时间  相似文献