DHA制品脂肪酸组成分析   总被引：2，自引：0，他引：2  

曾晓雄  孙怡 《湖南农业大学学报(自然科学版)》1996,22(5):489-492

运用ＧＣ和ＧＣ／ＭＳ分析了鱼油、多烯脂肪酸（ＰＵＦＡ）和ＤＨＡ奶粉中脂肪酸组成。结果表明以鱿鱼油制备的ＰＵＦＡ中主要脂肪酸是十六碳烯酸、十六碳酸、十八碳三烯酸、十八碳一烯酸、二十碳五烯酸、二十碳四烯酸、二十二碳六烯酸和二十二碳五烯酸；采用ＳＮＳ法制备的ＰＵＦＡ，（ＤＨＡ＋ＥＰＡ）含量可达９７．８０％，采用不同试剂组合处理，可使ＰＵＦＡ中ＤＨＡ，ＥＰＡ含量分别达到７７．７６％，７６．７１％；ＤＨＡ奶  相似文献   

Influence of Dietary Lipids and Environmental Salinity on the n-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis Capacity of the Marine Teleost Solea senegalensis     

Manuel Marrero   scar Monroig  Mnica Betancor  Marcelino Herrera  Jos A. Prez  Diego Garrido  Ana Galindo  Inmaculada Girldez  Covadonga Rodríguez 《Marine drugs》2021,19(5)

Fish vary in their ability to biosynthesise long-chain polyunsaturated fatty acids (LC-PUFA) depending upon the complement and function of key enzymes commonly known as fatty acyl desaturases and elongases. It has been reported in Solea senegalensis the existence of a Δ4 desaturase, enabling the biosynthesis of docosahexaenoic acid (DHA) from eicosapentaenoic acid (EPA), which can be modulated by the diet. The present study aims to evaluate the combined effects of the partial replacement of fish oil (FO) with vegetable oils and reduced environmental salinity in the fatty acid composition of relevant body compartments (muscle, hepatocytes and enterocytes), the enzymatic activity over α-linolenic acid (ALA) to form n-3 LC-PUFA through the incubation of isolated hepatocytes and enterocytes with [1-¹⁴C] 18:3 n-3, and the regulation of the S. senegalensis fads2 and elovl5 in the liver and intestine. The presence of radiolabelled products, including 18:4n-3, 20:4n-3 and EPA, provided compelling evidence that a complete pathway enabling the biosynthesis of EPA from ALA, establishing S. senegalensis, has at least one Fads2 with ∆6 activity. Dietary composition prevailed over salinity in regulating the expression of fads2, while salinity did so over dietary composition for elovl5. FO replacement enhanced the proportion of DHA in S. senegalensis muscle and the combination with 20 ppt salinity increased the amount of n-3 LC-PUFA in hepatocytes.  相似文献   

正常和白化褐牙鲆皮肤和肌肉中DHA和EPA含量的比较     

朱杰 《水产学报》2004,28(1):74-78

利用气相色谱法对相同养殖条件下生长的正常和白化褐牙鲆(Paralichthys olivaceus)的皮肤和肌肉组织中的DHA和EPA含量进行了比较分析。结果显示，正常和白化褐牙鲆的皮肤和肌肉中EPA含量相近，但白化个体皮肤中DHA含量显著低于正常个体，约为后者的1／2。而两者肌肉中的DHA含量则没有显著差异。同时，白化个体皮肤和肌肉组织中的DHA／EPA比值略低于正常个体。这一结果证明白化褐牙鲆皮肤蓄积或利用DHA的能力要低于正常褐牙鲆。关于鲆鳔类白化现象同鱼体对饵料中脂肪酸类物质的利用率之间的关系有待深入研究。  相似文献   

牙鲆幼鱼对EPA和DHA的营养需求   总被引：5，自引：2，他引：5  

薛敏 《水产学报》2004,28(3):285-291

研究了EPA和DHA水平对牙鲆生长的影响,饲料中含0.5%EPA和1.0%～1.5%DHA能保证牙鲆幼鱼最适生长,鱼体水分最低,肝体指数最小,脂肪含量有较大幅度提高,肝脏极性脂中EPA和DHA达到最大积累;在肝脏和肌肉的非极性脂部分,各组间的脂肪酸组成没有显著变化,而极性脂部分能体现出饲料中n-3 HUFA含量对鱼体脂肪酸组成的影响,极性脂中的EPA和DHA含量远高于非极性脂;在肌肉和肝脏的极性脂和非极性脂中都含有较高的16:0和18:1n-9; 18:1n-9/n-3HUFA可以作为必需脂肪酸满足程度的一个判据,18:1n-9值的升高往往是缺乏必需脂肪酸的表现,在生长最佳时18:1n-9/n-3HUFA比值下降,为0.62和0.74.  相似文献   

The effect of dietary n-3 HUFA levels and DHA/EPA ratios on growth, survival and osmotic stress tolerance of Chinese mitten crab Eriocheir sinensis larvae     

Liying Sui  Mathieu Wille  Patrick Sorgeloos 《Aquaculture (Amsterdam, Netherlands)》2007,273(1):139-150

The effect of varying levels of dietary n-3 highly unsaturated fatty acid (HUFA) and docosahexaenoic acid/eicosapentaenoic acid (DHA/EPA) ratios on growth, survival and osmotic stress tolerance of Eriocheir sinensis zoea larvae was studied in two separate experiments. In experiment I, larvae were fed rotifers and Artemia enriched with ICES emulsions with 0, 30 and 50% total n-3 HUFA levels but with the same DHA/EPA ratio of 0.6. In experiment II, larvae were fed different combinations of enriched rotifers and Artemia, in which, rotifers were enriched with emulsions containing 30% total n-3 HUFA, but different DHA/EPA ratio of 0.6, 2 and 4; while Artemia were enriched with the same emulsions, but DHA/EPA ratio of 0.6 and 4. In both experiments, un-enriched rotifers cultured on baker's yeast and newly-hatched Artemia nauplii were used as control diets. Larvae were fed rotifers at zoea 1 and zoea 2 stages; upon reaching zoea 3 stage, Artemia was introduced.Experiment I revealed no significant effect of prey enrichment on the survival of megalopa among treatments, but higher total n-3 HUFA levels significantly enhanced larval development (larval stage index, LSI) and resulted in higher individual dry body weight of megalopa. Furthermore higher dietary n-3 HUFA levels also resulted in better tolerance to salinity stress. Experiment II indicated that at the same total n-3 HUFA level, larvae continuously receiving a low dietary DHA/EPA ratio had significantly lower survival at the megalopa stage and inferior individual body weight at the megalopa stage, but no negative effect was observed on larval development (LSI). The ability to endure salinity stress of zoea 3, zoea 5 and megalopa fed diets with higher DHA/EPA ratio was also improved.  相似文献   

Vitamin B₆ supplementation increases the docosahexaenoic acid concentration of muscle lipids of rainbow trout (Oncorhynchus mykiss)     

Magda Maranesi  Mario Marchetti  Davide Bochicchio  & Luciana Cabrini 《Aquaculture Research》2005,36(5):431-438

Fish fillet quality is influenced by feed quality. In particular, vitamin B₆ availability improves growth rate and nutritional value, because it is related to protein and lipid metabolism. The present study investigates the effect of increasing amounts of vitamin B₆ on growth, fatty acid composition and lipid peroxidation of muscle tissue of rainbow trout (Oncorhynchus mykiss). Quadruplicate groups of trout of a mean initial weight of 110 g were fed commercial diets supplemented with four different quantities of vitamin B₆ (0, 10, 25 and 50 mg kg^?1 diet). Over the experimental period, there were no significant differences in weight gain and feed intake, while vitamin B₆ concentration of muscle was affected by dietary intake, even though its increase was not proportional to the concentrations in the feed. The fatty acid composition of muscle lipid showed differences between the four groups. The percentage of long‐chain unsaturated fatty acids, in particular docosahexaenoic acid, increased significantly in vitamin B₆‐supplemented groups. However, despite the increased fatty acid unsaturation index, lipid peroxidation parameters such as vitamin E content, malondialdehyde production, glutathione reductase and glutathione peroxidase activities did not show significant differences, meaning that the higher level of long‐chain unsaturated fatty acid did not increase the muscle susceptibility to oxidative stress.  相似文献   

The effects of dietary EPA and DHA fortification on lipolysis activity and physiological function in juvenile black sea bream Acanthopagrus schlegeli (Bleeker)   总被引：2，自引：0，他引：2  

A D Om  T Umino  H Nakagawa  T Sasaki  K Okada  M Asano  & A Nakagawa 《Aquaculture Research》2001,32(S1):255-262

  相似文献   

海水仔稚鱼对脂类的需求   总被引：5，自引：0，他引：5  

刘镜恪  雷霁霖 《齐鲁渔业》1996,13(1):19-21

脂类是海水仔稚鱼的必需营养要素，ｎ－３高度不饱和脂肪酸是海水仔稚鱼的必需脂肪酸，能直接影响海水仔稚鱼的生长和存活，其中又以ＤＨＡ和ＥＰＡ尤为重要。在仔稚鱼饵料中添加磷脂，也可提高仔稚鱼的生长和存活率。  相似文献   

Lipid composition of malpigmented and normally pigmented newly settled yellowtail flounder, Limanda ferruginea (Storer)     

L A Copeman  & C C Parrish 《Aquaculture Research》2002,33(15):1209-1219

The lipid composition of malpigmented (MP) and normally pigmented (NP), newly settled yellowtail flounder (Limanda ferruginea, Storer) was compared in order to elucidate a possible connection between lipids and pigmentation development. Larvae were fed commercially enriched live food for 12 weeks post hatch and then differences in lipid composition and size were analysed. NP fish were found to be significantly larger (standard length 35 mm) than MP fish (32 mm) at 100% settlement. There were higher proportions of triacylglycerols in NP fish (P = 0.01), whereas MP fish had an increased percentage of phospholipids (P = 0.01). NP fish had a higher percentage of docosahexaenoic acid (DHA) in the polar lipids of their body (P = 0.03) and total lipids of their eyes (P = 0.04). These data support previously proposed theories for the importance of DHA in pigmentation development. Principal components analysis (PCA) described the majority of the variance (77%) within the data set using just two principal components axes. PCA demonstrated that differences between body zones were greater than those between NP and MP fish within a given zone.  相似文献   

饵料中高不饱和脂肪酸在海水鱼苗培育中的作用     

黄磊詹勇  许梓荣 《齐鲁渔业》2003,20(12):10-13

大量研究表明，海水鱼苗对饵料中的某些高不饱和脂肪酸(HUFA)有特殊的需要，如果饵料中缺乏这些HUFA，如DHA(docoaahexaenoic acid 22：6n-3)、EPA(eicosapentaenoic acid 20：5n-3)和AA(arachidonic acid 20：4n-6)，将严重影响其营养价值。主要介绍了HUFA对海水鱼苗的营养和生理作用以及饵料中的各种HUFA水平在海水鱼苗培育中的作用，并且给出了某些海水鱼苗对饵料中HUFA的需要量和各种HUFA之间的适宜比例。  相似文献