水牛卵母细胞去核方法的比较     

陈自洪杨素芳  谢英李日聪  韦精卫石德顺 《中国兽医科技》2006,36(6):493-496

水牛卵母细胞体外成熟22h后，分别用盲吸法、点击法和纺锤体成像系统（Spindle—View System）去核。结果，三者的去核率分别为65．1％、81．8％和95．0％，差异极显著（P〈0．01）。以水牛胎儿成纤维细胞为供体，通过核移植构建的重构胚的融合率、分裂率和囊胚发育率，在上述3种方法之间均没有显著差异（P〉0．05）。认为点击法是一种较为简单、实用和有效的去核方法。  相似文献   

播种量对秋播苜蓿越年草产量的影响     

YU You-cheng ZHAO Yong-hong GUO Hai-jun LI Yu-yong 《家畜生态》2007,(5)

苜蓿播种量从10.0～45.0kg/hm~2设8个处理,研究不同播种量对越年草产量影响。结果表明:不同播种量对苜蓿越年草产量有显著影响,播种量10.0～35.0 kg/hm~2,苜蓿越年草产量随播种量增加而提高,播种量超过35.0 kg/hm~2,苜蓿越年草产量不再随播种量增加而提高;陕西关中农区苜蓿播种量控制在15.0～35.0 kg/hm~2,有利于提高其越年草产量。  相似文献   

卵母细胞去核及卵丘细胞移核方法对猪体细胞核移植的影响   总被引：1，自引：0，他引：1  

索永善  曹胜兰  李跃民 《黑龙江畜牧兽医》2006,(7):15-17

以猪体外成熟卵母细胞周围分离得到的卵丘细胞作为猪体细胞核移植的供核细胞,研究了卵母细胞不同去核方法(盲吸法、活性荧光染色去核法、盲吸法和活性荧光染色法联合去核法)的去核率差异,并比较了卵丘细胞透明带下注核和胞质内注核两种重建胚构建方法的胚胎裂解率、4 8细胞发育率和桑椹胚率的差异。结果表明:(1)以荧光染料染色法的去核率(87.18%)最高,与其他试验组相比差异显著(P<0.05);盲吸法去核所用时间最少(1.2 min/个),与其他试验组相比差异显著(P<0.05)。(2)透明带下注核比卵母细胞质内直接注核的卵裂率(40.11%/29.75%)和4 8细胞率(11.86%/6.81%)高,差异显著(P<0.05);桑椹胚率(1.75%/1.79%)无明显差异(P>0.05)。  相似文献   

Soybean sowing date effects on weed communities in untilled and tilled fields in north-eastern Japan     

HIROYUKI KOBAYASHI  ATSUSHI OYANAGI 《Weed Biology and Management》2006,6(3):177-181

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皖西南地区春播牧草的适应性研究     

陈程  谢俊龙  程学远  朱玉国  董召荣 《草原与草坪》2007,(2):5-9

对皖西南地区春播的9个牧草品种的适应性,草产量和营养成分进行分析,结果表明:墨西哥玉米、菊苣、杂交狼尾草、甜高粱产量高,干草产量和鲜草产量均在95 t/hm2和15 t/hm2以上,适应性强,品质优良,适合在皖西南地区大面积推广种植。苦荬菜和籽粒苋产量中等,蛋白质含量高达22%以上,青绿多汁,适口性好,可以做为优质牧草在皖西南地区大面积推广种植。饲用玉米、皖草2号、苏丹草产量较低,鲜草产量均在70 t/hm2以下,饲用玉米无再生性,皖草2号、苏丹草在高温条件下病虫害严重,种植时应该注意病虫害防治。  相似文献   

Expression of AFP and MXR7 mRNA in the peripheral blood and its relationship with prognosis of patients with hepatocellular carcinoma following liver transplantation     

YI Shu-hong  WANG Gen-shu  CAI Chang-jie  LU Min-qiang  YANG Yang  HU Bin  CHEN Gui-hua 《园艺学报》2007,23(11):2158-2163

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燕麦与毛苕子混播试验初报   总被引：3，自引：0，他引：3  

尚永成  马福 《青海草业》2000,(2)

通过对比试验的方法，进行了燕麦与毛苕子混播试验，结果表明：B处理鲜草产量明显高于其它处理，其抗倒伏性能随毛苕子播量的增加而降低。B处理的燕麦与毛苕子植株增高幅度也最大。  相似文献   

Effect of Relative Sowing Time on the Growth and Yield of the Component Crops in Okra/Maize and Okra/Cowpea Intercropping Systems     

C. O. Muoneke  J. E. Asiegbu  A. C. C. Udeogalanya 《Journal of Agronomy and Crop Science》1997,179(3):179-185

The effects of variations in the relative sowing time of component crops in okra/maize and okra/cowpea intercropping systems were examined in 1990 and 1991 cropping seasons. Okra was either sown the same day as, or two weeks before or after maize or cowpea. Intercropping reduced the growth and yield of okra, maize and cowpea relative to their sole crops. However, okra yield was depressed more by maize than by cowpea, especially when okra was sown two weeks after maize. Comparative assessment of okra/maize and okra/cowpea mixtures suggests that it is better to grow okra and cowpea together than intercropping okra and maize because yield advantages were always higher in okra/cowpea (67% and 59% in 1990 and 1991 respectively) than in okra/maize (15% and 29%). The results were discussed in light of competitive abilities of the various components in the mixtures.  相似文献   

Pearl Millet Phenology and Growth in Relation to Thermal Time under Arid Environment     

R. S. Singh  N. L. Joshi  H. P. Singh 《Journal of Agronomy and Crop Science》1998,180(2):83-91

Pearl millet ( Pennisetum glaucum L.R.Br.) hybrid MH 179 was grown under two moisture regimes viz., optimal moisture and rainfed conditions. The field experiment was continued for three consecutive rainy seasons to quantify the pearl millet development with thermal time. The possible influence of variation in natural sowing date on the relationships between crop development and thermal time have been described. At cardinal temperatures of 10°C (base temperature below which pearl millet development ceases), 33°C (optimal temperature for development) and 45°C (maximum temperature at and above which no development takes place), the crop required 1490–1794°Cd thermal time to reach physiological maturity. The thermal time requirement for different developmental stages was influenced by the sowing time and moisture availability during the growing season of the crop.
The leaf tip appearance on the main shoot of pearl millet in relation to thermal time was almost linear under both moisture conditions requiring about 44–50°Cd ± 2.6 °Cd leaf⁻¹, till the appearance of the flag (last) leaf. However, leaf tip appearance on primary tillers was slightly slower and required 53-58°Cd ± 4.7°Cd for each new leaf. Appearance of first primary tiller was later (at 320°Cd after emergence) under the rainfed condition as compared to the crop under the optimal moisture (at 250°Cd).Thereafter, the tiller appearance in relation to thermal time under both moisture conditions was at a linear rate of about 53-56°Cd ± 9.5°Cd tiller⁻¹.
Effect of microclimatic variations, canopy temperature, radiation and photoperiod on the phenology-thermal time relationships have been discussed under both the moisture conditions.  相似文献   

内蒙古阿拉善左旗4年采用机械模拟飞播牧草效益调查     

吴雨俊  郎海峰 《草业科学》1998,15(6):47-50

阿拉善左旗４年机械模拟飞播牧草累计面积为２９８０ｈｍ＾２，实播面积为２９８０ｈｍ＾２，１９９７年１０月调查，保存面积为２９７０ｈｍ＾２，保存面积率为９９．７％，４年来播区植被盖度提高８．９６倍，有苗面积率提高３１．５３倍，牧草产量提高３５．９１倍，第一性生产产值和第二性生产产值分别是飞播总投资的０．６７倍和０．９７倍，机械模拟飞播牧草表现出明显的经济效益、生产效益和社会效益。  相似文献