叶酸对2型糖尿病大鼠胸主动脉内皮功能的影响     

李卫红 肖强左春霞  商战平吴同果 《勤云标准版测试》2007,15(10):747-750

目的探讨长期补充叶酸对2型糖尿病大鼠胸主动脉内皮功能的影响及其作用机制。方法将2型糖尿病大鼠37只分为模型组(12只)、小剂量叶酸组(12只)和大剂量叶酸组(13只),另11只正常大鼠为正常对照组,叶酸干预11周后,剪尾采血分别检测血清一氧化氮、超氧化物歧化酶及丙二醛水平,并取胸主动脉制备主动脉环进行离体血管环反应性测定。结果模型组大鼠血清一氧化氮和超氧化物歧化酶水平明显低于正常对照组(P<0.01),而血清丙二醛水平明显高于正常对照组(P<0.01);补充叶酸11周后,小剂量叶酸组及大剂量叶酸组血清一氧化氮及超氧化物歧化酶水平均较模型组有明显增高(P<0.05或P<0.01),而血清丙二醛水平较模型组明显降低(P<0.01)。在每一个乙酰胆碱累积浓度下,模型组大鼠胸主动脉环的舒张度均较正常对照组明显减低(P<0.05或P<0.01),而小剂量叶酸组和大剂量叶酸组大鼠胸主动脉环的舒张度则均较模型组明显增高(P<0.05或P<0.01),小剂量叶酸组大鼠胸主动脉环的舒张度与大剂量叶酸组相比差异无显著性。结论长期补充叶酸对2型糖尿病大鼠血管内皮功能损伤具有明显预防作用,叶酸可能通过增加机体一氧化氮合成从而提高血清一氧化氮活性和提高机体抗氧化能力来预防2型糖尿病大鼠血管内皮功能损伤的发生。  相似文献   

Efficacy of turmeric on blood sugar and polyol pathway in diabetic albino rats   总被引：9，自引：0，他引：9  

Arun N  Nalini N 《Plant foods for human nutrition (Dordrecht, Netherlands)》2002,57(1):41-52

In the traditional system ofmedicine, Ayurveda, several spices and herbsare thought to possess medicinal properties. Amongthe spices, turmeric rhizomes (Curcumalonga. Linn.) are used as flavoring and coloringagents in the Indian diet everyday. In this research,we studied the effect of turmeric and itsactive principle, curcumin, on diabetes mellitus in arat model. Alloxan was used to induce diabetes.Administration of turmeric or curcumin to diabeticrats reduced the blood sugar, Hb and glycosylatedhemoglobin levels significantly. Turmeric andcurcumin supplementation also reduced the oxidativestress encountered by the diabetic rats. This wasdemonstrated by the lower levels of TBARS (thiobarbituric acid reactive substances), which mayhave been due to the decreased influx of glucose intothe polyol pathway leading to an increased NADPH/NADPratio and elevated activity of the potent antioxdiantenzyme GPx. Moreover, the activity of SDH (soorbitol dehydrogenase), whichcatalyzes the conversion of sorbitol to fructose, waslowered significantly on treatment with turmeric orcurcumin. These results also appeared to reveal thatcurcumin was more effective in attenuating diabetesmellitus related changes than turmeric.  相似文献   

罗格列酮对2型糖尿病患者血脂水平和血浆PAI-1活性的影响     

胡桂芳  武革  杨露 《湛江医学院学报》2008,26(3)

目的观察罗格列酮对2型糖尿病患者血脂水平和血浆纤溶酶原激活物抑制剂1(PAI-1)活性的影响。方法60例2型糖尿病患者随机分为常规治疗组和罗格列酮治疗组,每组30例,另选择20例健康体检者作为正常对照组。常规治疗组和罗格列酮治疗组分别于治疗前和治疗后12周检测空腹血糖(FPG)、血脂水平,采用发光底物法测定全部观察对象的PAI-1的活性。结果治疗12周后罗格列酮治疗组的血清甘油三酯(TG)下降、高密度脂蛋白胆固醇(HDL-C)升高,血浆PAI-1活性明显降低,差异有统计学意义(P<0.05)。结论罗格列酮可改善糖尿病患者的代谢紊乱,减少血浆PAI-1的表达,有助于改善或延缓糖尿病合并动脉粥样硬化的发生。  相似文献   

Apoptosis and its modulation in type Ⅰ diabetes mellitus     

DENG Li-juan  CHEN Shen-ren 《园艺学报》2002,18(9):1165-1168

Apoptosis, also known as programmed cell death, is a normal process contributing to tissue tumor during development and in the adult.During development apoptosis is involved in tissue homeostasis and eliminating nonfunctional, superfluous or harmful cells. It is an active process controlled by some gene and factors such as caspases, bcl-2, Fas/FasL and NO. Type 1 diabetes mellitus is regarded as a chronic autoimmune disease resulting from progressive destruction of insulin-producing β-cells in the pancreas. The relationship between apoptosis with its modulation and type 1 diabetes mellitus was reviwed.  相似文献   

Antidiabetic and Antioxidant Effects of Polyphenols in Brown Alga <Emphasis Type="Italic">Ecklonia stolonifera</Emphasis> in Genetically Diabetic KK-A<Superscript>y</Superscript> Mice   总被引：1，自引：1，他引：0  

Iwai K 《Plant foods for human nutrition (Dordrecht, Netherlands)》2008,63(4):163-169

The dietary intake and control of blood glucose levels are very important in hyperglycemic patients and α-glucosidase inhibitors are a cost-effective means to preventing the progression of diabetes. In search of a natural inhibitor from food materials, α-glucosidase inhibitory activity and the anti-hyperglycemic effects of a brown alga, Ecklonia stolonifera, were investigated using non-insulin dependent diabetic mice. Methanolic extract of E. stolonifera (MEE), which contains a high content of polyphenols, showed strong inhibition of α-glucosidase in vitro. Male KK-A^y mice, a genetically non-insulin dependent diabetic model, showed hyperglycemia with aging, but the ingestion of MEE suppressed the increase in plasma glucose and lipid peroxidation levels in unfasted KK-A^y mice dose dependently. In KK-A^y mice, which were fed the MEE diet for 4 weeks, MEE moderated the elevation of plasma glucose levels after the oral administration of maltose. The polyphenols in MEE were estimated to be phlorotannins by HPLC-PDA and LC/MS analyses. These results demonstrate that E. stolonifera, seaweed typically used as a health food, has strong antidiabetic and antioxidant effects in vivo, thus, it may have beneficial properties in the prevention of diabetes and could be useful in the development of an antidiabetic pharmaceutical and functional food.  相似文献   

Aqueous extract of Anemarrhena rhizome increases cell proliferation and neuropeptide Y expression in the hippocampal dentate gyrus on streptozotocin-induced diabetic rats     

Shin MS  Kim SK  Kim YS  Kim SE  Ko IG  Kim YS  Kim CJ  Kim YM  Kim BK  Kim TS 《Fitoterapia》2008,79(5):323-327

In this study, the effects of the aqueous extract of Anemarrhena rhizome on cell proliferation and neuropeptide Y expression in the dentate gyrus of streptozotocin-induced diabetic rats were investigated via immunohistochemistry for 5-bromo-2'-deoxyuridine (BrdU) and neuropeptide Y. The results showed that the treatment with 50 to 200 mug/kg/day for 7 days of the aqueous extract of Anemarrhena rhizome increased new cell formation and neuropeptide Y expression in the dentate gyrus of diabetic rats reduced by the treatment with streptozotocin in rat.  相似文献   

Study on the variation of trace elements and oxidative stress in diaphragm of the diabetic rats     

SHEN Xing-ping  SHU Chang-da  HE Jun 《园艺学报》2000,16(7):655-657

AIM:To investigate the relationship between trace elements and oxidative stress in diabetic diaphragm. METHODS: Contents of copper(Cu), manganese(Mn), zinc(Zn), iron(Fe),cadmium(Cd),chromium(Cr),cobalt(Co),molybdenum(Mo) and lithium(Li) in the alloxan-induced diabetic rat diaphragm muscles were measured by atomic absorption spectrophotometer, andtheir superoxide dismutase(SOD) activities and malondiadehyde(MDA) contents were detected. RESULTS: The contents of Cu, Zn, Cr and Li were lower and the Fe and Cd were higher in diabetic rat diaphragm muscles than that of the control group, but there were no significant differences in Mn, Co and Mo, between control and diabetic group. SOD activities decreased significantly and MDA contents increased significantly in diaphragm of diabetic rats as compared with the control group.In addition,in diabetic rats diaphragm, there was a positive correlation between Zn and SOD, and negative correlation between Zn and MDA, and Cd and SOD. CONCLUSION: Changes of the contents of Cu, Zn, Fe, Cd, Cr, Li and the increase of oxidative stress in diaphragm were found in the early diabetes, and Zn, Cd play an importment role in oxidative stress of diabetic diaphragm.  相似文献   

栀子苷的降糖作用和对PPARγ受体的激活   总被引：1，自引：0，他引：1  

颜静恩  李晚忱  吕秋军  卞广兴  温利青  任建平 《四川农业大学学报》2007,25(4):415-418

葡萄糖消耗试验表明,栀子苷能显著促进前脂肪细胞对葡萄糖的吸收。小鼠荷糖试验和四氧嘧啶致糖尿病小鼠血糖试验证实,栀子苷具有剂量依赖性降低小鼠餐后血糖及糖尿病高血糖的作用。通过基于PPARγ受体信号通路的报告基因诱导表达试验,发现栀子苷在体内外的降糖功效,可能与过氧化物酶增殖体激活受体γ的激活有关。  相似文献   

Plasma leptin concentration in dogs: effects of body condition score, age, gender and breeds     

Ishioka K  Hosoya K  Kitagawa H  Shibata H  Honjoh T  Kimura K  Saito M 《Research in veterinary science》2007,82(1):11-15

Leptin is a cytokine produced by adipocytes, and plays a key role in the regulation of energy balance. In the present study, we measured plasma leptin concentrations of 166 normal and obese dogs visiting veterinary practices, and clarified the influence of age, gender and breed on plasma leptin levels in dogs. Leptin levels were higher in the dogs with higher body condition scores. There was no noticeable influence of age, gender and breed, but those in optimal puppies and obese Miniature Dachshund tended to be lower than those in corresponding groups. We conclude that plasma leptin is a reliable marker of adiposity in dogs regardless of age, gender and breed variations, and thereby useful as a blood biochemistry test for health examinations and treatment of obesity.  相似文献   

Type 2 diabetes mellitus with pancreatic β cell dysfunction in 3 horses confirmed with minimal model analysis     

A. E. DURHAM  K. J. HUGHES  H. J. COTTLE  Dr. D. I. RENDLE  R. C. BOSTON 《Equine veterinary journal》2009,41(9):924-929

Reasons for performing study: Type 2 diabetes mellitus (T2DM) is diagnosed rarely in equine practice although it may be under‐recognised. A greater awareness of the condition and therapeutic considerations would be to the benefit of such cases presenting in practice. More investigation into the pharmacological management of these cases is needed. Objectives: Three cases of diabetes mellitus were investigated using a specific test for insulin sensitivity and pancreatic β cell function in order to define accurately and characterise the existence of T2DM in all 3 subjects. Methods: The insulin‐modified frequently sampled i.v. glucose tolerance test was performed in each case and the data so obtained were subject to minimal model analysis of insulin‐glucose dynamics. Cases were then monitored following treatment using a combination of dietary modification, metformin, glibenclamide and pergolide. Results: Marked insulin resistance was identified in each case and, furthermore, severe pancreatic β cell dysfunction was present therefore classifying each case as end stage T2DM. Treatment was nevertheless associated with restoration of normoglycaemia in all cases. Conclusions: T2DM in horses may be more common than generally considered. In some cases individuals may respond to therapy aimed at restoring insulin sensitivity and pancreatic function. Drugs used in other species for the treatment of T2DM have not yet been adequately tested in horses. Potential relevance: T2DM should be considered as an important differential diagnosis in mature to elderly horses and ponies suffering from weight loss, polydipsia and polyuria. Clinicians should be encouraged to offer treatment and management advice when such cases are encountered.  相似文献