香蕉茎秆单宁酸提取纯化及其抗氧化性   总被引：1，自引：0，他引：1  

徐树英  谭蔚  张玉苍 《热带作物学报》2016,37(8):1567-1574

以香蕉茎秆为原料,采用溶剂提取法进行单宁酸提取,在单因素试验设计的基础上,通过正交试验优化香蕉茎秆单宁酸提取工艺,采用大孔树脂吸附技术对粗提物进行分离纯化。纯化物采用傅里叶红外光谱(FTIR)测定其官能团,通过凝胶渗透色谱(GPC)测定纯化物的分子量及分子量分布。通过测定其清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基的能力,考察香蕉茎秆单宁酸的抗氧化性。结果表明:香蕉茎秆单宁酸最佳提取工艺为:乙醇浓度50%,提取时间90 min,料液比1∶11,提取温度50℃,在此条件下单宁酸提取量为17.9 mg/g。纯化工艺为:5 mg/m L上样,流速为1.5 m L/min过AB-8型大孔树脂柱,最后用60%乙醇洗脱。FTIR结果表明,该纯化物为多羟基酚酯类化合物;GPC结果显示香蕉茎秆单宁酸分子质量在3.43×10~4~4.43×10~4之间。经过DPPH法测出样品浓度达到0.6 mg/m L时,其DPPH自由基清除率为86.59%。  相似文献   

Function of sodium and potassium in growth of sodium-loving Amaranthaceae species     

Mina Yamada  Chika Kuroda  Hideyasu Fujiyama 《Soil Science and Plant Nutrition》2016,62(1):20-26

We observed that the growth of three Amaranthaceae species was promoted by sodium (Na), in the order dwarf glasswort (Salicornia bigelovii Torr.) >> Swiss chard (Beta Burgaris L. spp. cicla cv. Seiyou Shirokuki) > table beet (Beta vulgaris L. spp. vulgaris cv. Detroit Dark Red). In the present study, these Na-loving plants were grown in solutions containing 4 mol m^?3 nitrate nitrogen (NO₃-N) and 100 mol m^?3 sodium chloride (NaCl) and potassium chloride (KCl) under six Na to potassium (K) ratios, 0:100, 20:80, 40:60, 60:40, 80:20 and 100:0, to elucidate the function of Na and K on specific characteristics of Na-loving plants. The growth of dwarf glasswort increased with increasing Na concentration of the shoot, and the shoot dry weight of plants grown in 100:0 Na:K was 214% that of plants grown at 0:100. In Swiss chard and table beet, growth was unchanged by the external ratio of Na to K. The water content was not changed in Swiss chard or table beet by the external Na to K ratio. These observations indicate that both Na and K have a function in osmotic regulation. However, dwarf glasswort could not maintain succulence at 0:100; therefore, Na has a specific function in dwarf glasswort for osmotic regulation to maintain a favorable water status, and the contribution of K to osmotic regulation is low. NO₃-N uptake was promoted by Na uptake in dwarf glasswort and Swiss chard. NO₃-N uptake and transport to shoots was optimal at 100:0 in dwarf glasswort and at 80:20 in Swiss chard. These functions are very important for the Na-loving mechanism, and the contribution of K was lower in dwarf glasswort than in Swiss chard.  相似文献   

香蕉和土壤中吡唑醚菌酯的残留分析     

赵方方  张月  吕岱竹  谢德芳 《热带作物学报》2015,36(9):1694-1700

建立吡唑醚菌酯在香蕉全蕉、蕉肉及土壤中的残留分析方法,测定吡唑醚菌酯在云南、海南两地香蕉及土壤中的残留动态及最终残留。结果表明：在0.01~0.1 mg/kg添加范围内,吡唑醚菌酯在香蕉果、肉、土中的平均回收率为82％~104％,变异系数为1.3％~3.6％;方法最小检出量为1×10-10 g,最低检出浓度为0.01 mg/kg。吡唑醚菌酯在云南和海南两地香蕉中的半衰期分别为17.2、16.7 d,在土壤中的半衰期分别为19.9、17.9 d。施药后35、42、49 d收获的香蕉中吡唑醚菌酯残留量均低于0.02 mg/kg。该方法准确度高,灵敏度高,线性良好。  相似文献   

香蕉分化芽BBTV DAS-ELISA检测方法的建立和应用     

周朋  余乃通  章绍延  王健华  胡加谊  王祥  梁洁  刘志昕 《植物保护》2014,40(5):117-120

为了解海南海口香蕉分化芽携带香蕉束顶病毒(Banana bunchy top virus, BBTV)的情况, 本研究建立了双抗夹心酶联免疫反应(DAS-ELISA)检测法, 对来自海南地区组培厂的香蕉分化芽进行检测。检测结果表明, 6个品种1 852个香蕉分化芽平均带毒率为2.1%, 其中‘皇帝蕉’为1.98%, ‘粤科1号’为2.07%, ‘广粉蕉’为1.98%, ‘大蕉’为6.25%, ‘218’为1.89%, ‘巴西蕉’为2.25%。为了进一步验证ELISA结果的可靠性, 随机选取12个阳性样品提取总DNA进行PCR鉴定。鉴定结果显示, 12个样品中11个检测出有BBTV, 表明DAS-ELISA方法的准确率较高, 与分子检测结果基本一致, 可以胜任日常香蕉组培苗BBTV的检测。  相似文献   

Molecular characterization of a cold-responsive RING-H2 finger gene from banana fruit and its interaction with MaMYC2a     

《Postharvest Biology and Technology》2014

  相似文献   

A novel technique using 1-MCP microbubbles for delaying postharvest ripening of banana fruit     

《Postharvest Biology and Technology》2014

This study aimed to investigate the application of microbubble technology for delaying banana ripening. A preparation of 1-MCP designed for use as a form of aqueous micro bubble (MBs) solutions was formulated. Banana fruit were immersed in 500 nL L⁻¹ of aqueous 1-MCP microbubbles (1-MCP-MBs) or fumigated with 500 nL L⁻¹ 1-MCP, then stored at 25 °C for 8 days. 1-MCP-MBs were more effective in delaying postharvest ripening than conventional 1-MCP fumigation. 1-MCP-MBs reduced the respiration rate and ethylene production compared to the control and 1-MCP fumigated fruit. Moreover, 1-MCP-MBs delayed yellowing and maintained firmness of banana fruit during storage. These results indicate that 1-MCP-MBs can be used as an alternative method for delaying the postharvest ripening of banana fruit, and its application for other commodities needs to be further elucidated.  相似文献   

Effects of sika deer on vegetation in Japan: A review     

Seiki Takatsuki 《Biological conservation》2009,142(9):1922-1929

The Japanese sika deer Cervus nippon has expanded its range by nearly 70% during the last two decades. Browsing by sika deer affects vegetation in both agricultural and forested habitats. Effects of sika deer on vegetation are conspicuous on deer-inhabited islands: forest structure and composition are altered by deer grazing and browsing, and consequently regeneration is prevented. By felling of old trees, forest gaps are formed, but since sapling recruitment is prevented, shade intolerant plants invade. Unpalatable forbs like ragwort Senecio cannabifolius and ferns like bracken Pteridium aquilinum (Dennstaedtiaceae) become dominants in open habitats. At the places of highest deer density, the Zoysia japonica community, a low growing grass mat, develops. Sika deer function as a seed dispersal agent of this grass. Indirect effects of sika deer are not well studied, but some studies have shown reduction of understory bamboo cover, which in turn improves the survival of tree seedlings and declines of wood mice Apodemus spp. Japanese forests in lower mountainous areas were widely logged during the 1940s and 1950s. These areas were not well planted during the war and until 1950, but thereafter intensively planted to alter to conifer plantation as a nationwide campaign. For several decades after the war, rodents and hares grazed planted trees. According to growth of the planted trees to form canopy, which is unfavorable for rodents and hares, their damage declined. After the 1960s, old-growth forests in high mountainous areas were logged, and conifers were planted. Animals causing forestry damage were replaced by sika deer during the 1980s. Sika deer eat a wide variety of foods, and are gregarious, which causes heavy impacts on vegetation. Effects of sika deer are expanding to natural forests, alpine vegetation, and marshes. To reduce damages on forestry and natural vegetation, as many as 100,000 sika deer are culled every year. However, the hunter population is rapidly declining, and it is expected population control by culling will be insufficient. Although sika deer are an important component of Japanese forests, current population densities exceed the capacity of many ecosystems to tolerate herbivory.  相似文献   

钾钙镁营养对香蕉产量、品质及贮藏性的影响   总被引：5，自引：0，他引：5  

杨苞梅  李进权  姚丽贤  李国良  何兆桓  周昌敏  涂仕华 《中国生态农业学报》2010,18(2):290-294

香蕉是嗜K作物,养分需求最大的4种元素为KNCaMg,而K、Ca、Mg三者间存在交互作用。本文研究了不同K、Ca、Mg营养条件下香蕉产量、品质及其贮藏性的变化,结果表明:适量配施K、Ca、Mg肥能有效促进香蕉果实的生长发育,提高香蕉果实品质及产量;有效降低香蕉果皮多酚氧化酶活性、果皮丙二醛含量和果肉淀粉酶活性,减少贮藏过程中的重量损失,延缓香蕉果实的衰老进程,提高香蕉果实的耐贮藏性。在广东省高州市香蕉产区,为实现香蕉高产优质耐贮藏,适宜施肥量为K2O990kg·hm-2,Mg37.5kg·hm-2,Ca90.0kg·hm-2。  相似文献   

香蕉化肥农药减量化模式和配套技术研究     

匡石滋  田世尧  李春雨  易干军  洪炎龙  万国富 《杂粮作物》2011,(3)

介绍了适宜珠三角地区的香蕉化肥农药减量化模式,并提出了香蕉平衡施肥、病虫草害综合治理、香蕉间作栽培、香蕉废弃茎叶循环利用等4方面的配套技术,模式与技术示范显示出良好的效果。  相似文献   

“天宝香蕉”产业化技术模式应用与成效分析   总被引：1，自引：0，他引：1  

黄达斌 《中国农学通报》2009,25(13):234-240

芗城区为打好“天宝香蕉”品牌,推动产业提质增效和可持续发展,通过项目实施,培育龙头企业作为项目实施载体,形成“龙头企业+农户+基地+标准”的技术模式,采用先进配套设备,搞好产业化经营,提高组织化程度;研究制定福建省地方标准《天宝香蕉》标准文本,应用利益驱动原则,引导农户加快标准化生产技术的应用推广,确保香蕉品质优化与食品安全,“天宝香蕉”获国家地理标志保护产品。项目实施采取加强组织领导,建立示范基地,健全科技推广网络,开展优质科技服务。两年来,示范面积152.7hm2,推广面积2066.7hm2,平均产量34932 kg/hm2,新增产量1.17万t,新增产值3529.1万元;节省成本373.6万元;产投比2.6。对推动福建省发展优质、丰产、安全、高效的香蕉产业具有重要意义。  相似文献